Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 389
Primeira ... 123456789 ... Última
Imagem não selecionada

Imprime registro no formato completo
16S rRNA based identification of _Aeromonas sp. kumar_ by constructing phylogenetic tree and identification of regulatory elements from the harmful Red Tide bloom, Gulf of Mannar Nature Precedings
P. Kumar; C. M. Ramakritinan; A. K. Kumaraguru.
A bacterial strain, designated _Aeromonas sp. kumar_, was isolated from a water sample collected from Red tide Bloom occurred in the region of Gulf of Mannar region, Puthumadam Coast, India and the strain was identified using 16S rRNA based identification. During the sample collection, microbiology analysis was done to study the morphology of the bacteria. Pure culture of strain was maintained through out the study. DNA was isolated and sequenced using 16S rRNA primers. A length of 1452 nucleotide was sequenced and was put in public data base for obtaining accession number. The sequence was studied using MEGA 4, to estimate the evolutionary distances and to construct the Phylogenetic tree. Along with that Regulatory elements and Transcription factors were...
Tipo: Manuscript Palavras-chave: Ecology; Microbiology; Bioinformatics.
Ano: 2009 URL: http://precedings.nature.com/documents/3898/version/1
Imagem não selecionada

Imprime registro no formato completo
2011 German Escherichia coli O104:H4 outbreak: Alignment-free whole-genome phylogeny by feature frequency profiles Nature Precedings
Man Kit Cheung; Lei Li; Wenyan Nong; Hoi Shan Kwan.
Accuracy of SNP-based whole-genome phylogeny reconstruction relies heavily on quality of sequence alignment which is particularly hindered by poorly assembled genomes. Alignment-free methods might provide additional insights. Here, we constructed a whole-genome phylogeny of 10 isolates from the current German E. coli outbreak against 30 existing E. coli genomes as well as that of a historical EHEC isolate using the alignment-free feature frequency profile method. Our results revealed a high similarity among E. coli isolates from the current outbreak and the historical EHEC being the most closely related isolate sequenced thus far.
Tipo: Manuscript Palavras-chave: Genetics & Genomics; Microbiology; Bioinformatics; Evolutionary Biology.
Ano: 2011 URL: http://precedings.nature.com/documents/6109/version/2
Imagem não selecionada

Imprime registro no formato completo
2011 German Escherichia coli outbreak: Alignment-free whole-genome phylogeny by feature frequency profiles Nature Precedings
Man Kit Cheung; Lei LI; Wenyan Nong; Hoi Shan Kwan.
Accuracy of SNP-based whole-genome phylogeny reconstruction relies heavily on quality of sequence alignment which is particularly hindered by poorly assembled genomes. Alignment-free methods might provide additional insights. Here, we constructed a whole-genome phylogeny of 9 E.coli isolates from the 2011 German outbreak against existing E. coli genomes using the alignment-free feature frequency profile method. In addition, we looked for gene elements that distinguish the outbreak group from the other E. coli strains and possibly accounted for the emergence of the outbreak isolates using the distinguishing feature analysis.
Tipo: Manuscript Palavras-chave: Genetics & Genomics; Microbiology; Bioinformatics; Evolutionary Biology.
Ano: 2011 URL: http://precedings.nature.com/documents/6109/version/1
Imagem não selecionada

Imprime registro no formato completo
2011 German Escherichia coli outbreak: Prophage analysis of close-assembled TY2482 against 55989 using PHAST Nature Precedings
Man Kit Cheung; Lei LI; Wenyan Nong; Hoi Shan Kwan.
Using the Hiseq data of the German E. coli outbreak isolate TY2482, preliminary prophage analyses have been performed by some researchers previously. With the closed assembly of the same isolate being available, another round of analysis might help in resolving questions that remain unclear due to the incompleteness of the dataset.
Tipo: Manuscript Palavras-chave: Genetics & Genomics; Microbiology; Bioinformatics.
Ano: 2011 URL: http://precedings.nature.com/documents/6110/version/1
Imagem não selecionada

Imprime registro no formato completo
A Bacterial Enzyme Catalyzing Double Reduction of a β,δ-Diketo Ester with Unprecedented Stereoselectivity Nature Precedings
Xuri Wu; Nan Liu; Yunmian He; Yijun Chen.
Various ketoreductases exclusively participate in all common biological events, and they are a class of important biocatalysts for the production of chiral alcohols. While many types of ketoreductase have been extensively studied and their functions, properties and utilities have been well known, the capability of stereoselectively reducing two carbonyl groups in the same diketohexanoate ester molecule to form a dihydroxy product by a single ketoreductase has not been evidently characterized. Here we show that a unique and novel enzyme, diketoreductase, was cloned from Acinetobacter baylyi, heterogeneously expressed in _Escherichia coli_ and purified to homogeneity. The diketoreductase is up to 78% homologous to bacterial 3-hydroxyacyl coenzyme-A...
Tipo: Manuscript Palavras-chave: Biotechnology; Chemistry; Microbiology.
Ano: 2008 URL: http://precedings.nature.com/documents/1697/version/1
Imagem não selecionada

Imprime registro no formato completo
A Bacterial Enzyme Catalyzing Double Reduction of a β,δ-Diketo Ester with Unprecedented Stereoselectivity Nature Precedings
Xuri Wu; Nan Liu; Yunmian He; Yijun Chen.
Various ketoreductases exclusively participate in all common biological events, and they are a class of important biocatalysts for the production of chiral alcohols. While many types of ketoreductase have been extensively studied and their functions, properties and utilities have been well known, the capability of stereoselectively reducing two carbonyl groups in the same diketohexanoate ester molecule to form a dihydroxy product by a single ketoreductase has not been evidently characterized. Here we show that a unique and novel enzyme, diketoreductase, was cloned from Acinetobacter baylyi, heterogeneously expressed in _Escherichia coli_ and purified to homogeneity. The diketoreductase is up to 78% homologous to bacterial 3-hydroxyacyl coenzyme-A...
Tipo: Manuscript Palavras-chave: Biotechnology; Chemistry; Microbiology.
Ano: 2010 URL: http://precedings.nature.com/documents/1697/version/2
Imagem não selecionada

Imprime registro no formato completo
A laboratory model system for analysing microbial interactions occurring in anoxic estuarine sediments. ArchiMer
Herbert, R; Keith, S.
Defined mixed populations of Clostridium butyricum, Desulfovibrio desulfuricans and Chromatium vinosum co-existed for long periods when grown in a single stage chemostat with glucose as sole carbon source. The nature and availability of the inorganic nitrogen source exerted a major effect on carbon flow in the experimental system. The data show that both the C. butyricum and D. desulfuricans isolates can utilise NO sub(3) as an e super(-)acceptor with an increase in cell yield. Under these growth conditions the free S super(2-) levels were lower resulting in more stable mixed populations. In addition the data show that the C. butyricum produces more oxidised fermentation end-products (acetate) when NO sub(3) super(-) was available and more reduced products...
Tipo: Text Palavras-chave: Chromatium vinosum; Desulfovibrio desulfuricans; Clostridium butyricum; Biogeochemistry; Models; Bacteria; Detritus; Mineralization; Anoxic sediments; Estuarine sedimentation; Analytical techniques; Carbon cycle; Interspecific relationships; Microbiology.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-947.pdf
Imagem não selecionada

Imprime registro no formato completo
A new multicompartmental reaction-diffusion modeling method links transient membrane attachment of E. coli MinE to E-ring formation Nature Precedings
Satya N. V. Arjunan; Masaru Tomita.
Many important cellular processes are regulated by reaction-diffusion (RD) of molecules that takes place both in the cytoplasm and on the membrane. To model and analyze such multicompartmental processes, we developed a lattice-based Monte Carlo method, Spatiocyte that supports RD in volume and surface compartments at single molecule resolution. Stochasticity in RD and the excluded volume effect brought by intracellular molecular crowding, both of which can significantly affect RD and thus, cellular processes, are also supported. We verified the method by comparing simulation results of diffusion, irreversible and reversible reactions with the predicted analytical and best available numerical solutions. Moreover, to directly compare the localization...
Tipo: Manuscript Palavras-chave: Developmental Biology; Microbiology; Bioinformatics.
Ano: 2009 URL: http://precedings.nature.com/documents/3845/version/1
Imagem não selecionada

Imprime registro no formato completo
A new outlook towards kidney injuries Nature Precedings
Mahesh Satwekar; Abhijeet Satwekar.
Acute and chronic progression of injury to the kidney leads to the failure of the renal system and has become an increasingly important cause of morbidity and mortality. Present diagnosis detects the condition only after irreversible loss of 70 percent of kidney function. Current research is focused only on the clinical manifestations after the kidney injuries and not towards the exact cause of the condition. Here we propose a new outlook- that there is an involvement of a pathogen in the pathogenesis of kidney injuries. Basis for our proposal is given by the similarity of the pathogenesis events occurring between a classical example of hepatitis and kidney injuries. Furthermore, literature regarding the role of early kidney injury biomarkers in innate...
Tipo: Manuscript Palavras-chave: Biotechnology; Immunology; Microbiology.
Ano: 2012 URL: http://precedings.nature.com/documents/7021/version/1
Imagem não selecionada

Imprime registro no formato completo
A potential cyanobacterial ancestor of Viridiplantae chloroplasts Nature Precedings
Wriddhiman Ghosh; Prabir Haldar; Sabyasachi Bhattacharya; Jaideb Chatterjee; Prosenjit Pyne; Masrure Alam.
The theory envisaging the origin of plastids from endosymbiotic cyanobacteria is well-established but it is difficult to explain the evolution (spread) of plastids in phylogenetically diverse plant groups. It is widely believed that primordial endosymbiosis occurred in the last common ancestor of all algae^1^, which then diverged into the three primary photosynthetic eukaryotic lineages, viz. the Rhodophyta (red algae), Glaucocystophyta (cyanelle-containing algae) and Viridiplantae (green algae plus all land plants)^2^. Members of these three groups invariably have double membrane-bound plastids^3^, a property that endorses the primary endosymbiotic origin of the organelles. On the other hand, the three or four membrane-bound plastids of the evolutionary...
Tipo: Manuscript Palavras-chave: Microbiology; Evolutionary Biology.
Ano: 2011 URL: http://precedings.nature.com/documents/6607/version/1
Imagem não selecionada

Imprime registro no formato completo
A Proposal of Genomic Analytical Workflow in a Bacterial Pathogen Outbreak Investigation Nature Precedings
Hoi Shan Kwan; Chun Hang Au; Chi Keung Cheng; Man Kit Cheung; Qianli Huang; Lei Li; Wenyan Nong; Man Chun Wong.
The German Escherichia coli O104:H4 outbreak causing Hemolytic Uremic Syndrome (HUS) started in early May 2011. Conventional typing methods including serotyping, multi-locus sequence typing (MLST) and pulse-field gel electrophoresis (PFGE) seemed to yield only limited information about the causative agent. The advantages of the Genome sequencing approach are apparent compared to conventional methods. Genome sequencing and analyses with an appropriate workflow would be the new paradigm in bacterial outbreak investigation.
Tipo: Manuscript Palavras-chave: Biotechnology; Genetics & Genomics; Microbiology.
Ano: 2011 URL: http://precedings.nature.com/documents/6663/version/1
Imagem não selecionada

Imprime registro no formato completo
A Quantitative Approach to Investigating the Hypothesis of Prokaryotic Intron Loss Nature Precedings
Robert M. Sinclair.
Using a novel method, we show that ordered triplets of motifs usually associated with spliceosomal intron recognition are underrepresented in the protein coding sequence of complete Thermotogae, archaeal and bacterial genomes. The underrepresentation observed does not extend to the noncoding strand, suggesting that the cause of the asymmetry is related to mRNA rather than DNA. Our data do not suggest that the underrepresentation is due to gene transfer from eukaryotes. We speculate that one possible explanation for these observations is that the protein coding sequence of Thermotogae, Archaea and Bacteria was at some time in the past subjected to selection against certain motifs appearing in an order which might initiate splicing in environments harboring...
Tipo: Manuscript Palavras-chave: Genetics & Genomics; Microbiology; Bioinformatics; Evolutionary Biology.
Ano: 2011 URL: http://precedings.nature.com/documents/5770/version/1
Imagem não selecionada

Imprime registro no formato completo
A Report of the Mosquito Host Range of Pythium guiyangense Su Nature Precedings
Xiaoqing Su.
Mosquito causes problems in both disease transmission and disturbance to people's daily life. The controlling methods used now are mainly application of chemical pesticides which result in serious harm to environment. Biological control of the pest is one of the ideal ways. But, lack of suitable agent makes it difficult to extend this technique in wider scope. _Pythium guiyangense_ Su is a newly found fungal pathogen of mosquitoes and is promising for practical use. Here we summarize a list of mosquitoes that can be infected by the fungus so far, which shows the potential significance of the application of the fungus in the future.
Tipo: Manuscript Palavras-chave: Microbiology; Earth & Environment.
Ano: 2008 URL: http://precedings.nature.com/documents/1875/version/1
Imagem não selecionada

Imprime registro no formato completo
A Simple, Rapid Method for Extracting Large Plasmid DNA from Bacteria Nature Precedings
Spencer D. Heringa; Jonathan D. Monroe; James B. Herrick.
We are studying the lateral transfer of transmissible antibiotic resistance plasmids among stream bacteria impacted by fecal runoff from poultry and cattle. Such plasmids are typically large (ca. 40 – 100 kb) and occur in low copy numbers in the cell and have therefore typically been difficult to isolate and therefore to study. Traditional protocols, based upon variations of the standard alkaline-lysis method, are long (ca. 1 1/2 to 2 days) and difficult. Commercial kits designed for the isolation of Baterial Artificial Chromosomes (BACs) can be used and are an improvement; however, these are expensive and still require hours of sustained effort. We have adapted a method published by Rondon et al. (1999), originally designed for the isolation of...
Tipo: Poster Palavras-chave: Biotechnology; Genetics & Genomics; Microbiology.
Ano: 2007 URL: http://precedings.nature.com/documents/1249/version/1
Imagem não selecionada

Imprime registro no formato completo
A Unifying Scenario on the Origin and Evolution of Cellular and Viral Domains Nature Precedings
Claudiu I. Bandea.
The cellular theory on the nature of life has been one of the first major advancements in biology. Viruses, however, are the most abundant life forms, and their exclusion from mainstream biology and the Tree of Life (TOL) is a major paradox in biology. This article presents a broad, unifying scenario on the origin and evolution of cellular and viral domains that challenges the conventional views about the history of life and supports a TOL that includes viruses. Co-evolution of viruses and their host cells has led to some of the most remarkable developments and transitions in the evolution of life, including the origin of non-coding DNA as a genomic protective device against viral insertion damage. However, one of the major fundamental evolutionary...
Tipo: Manuscript Palavras-chave: Genetics & Genomics; Immunology; Microbiology; Evolutionary Biology.
Ano: 2009 URL: http://precedings.nature.com/documents/3888/version/1
Imagem não selecionada

Imprime registro no formato completo
Accurate Reconstruction of Molecular Phylogenies for Proteins Using Codon and Amino Acid Unified Sequence Alignments (CAUSA) Nature Precedings
Xiaolong Wang; Yu Fu; Yue Zhao; Qi Wang; Chandra Sekhar Pedamallu; Shuang-yong Xu; Yingbo Niu; Jingjie Hu.
Based on molecular clock hypothesis, and neutral theory of molecular evolution, molecular phylogenies have been widely used for inferring evolutionary history of organisms and individual genes. Traditionally, alignments and phylogeny trees of proteins and their coding DNA sequences are constructed separately, thus often different conclusions were drawn. Here we present a new strategy for sequence alignment and phylogenetic tree reconstruction, codon and amino acid unified sequence alignment (CAUSA), which aligns DNA and protein sequences and draw phylogenetic trees in a unified manner. We demonstrated that CAUSA improves both the accuracy of multiple sequence alignments and phylogenetic trees by solving a variety of molecular evolutionary problems in...
Tipo: Manuscript Palavras-chave: Genetics & Genomics; Microbiology; Bioinformatics; Evolutionary Biology.
Ano: 2011 URL: http://precedings.nature.com/documents/6730/version/1
Imagem não selecionada

Imprime registro no formato completo
Activity of Exoenzymes in Treated Wastewater Irrigated Soils Nature Precedings
Elisabeth Jüschke; Bernd Marschner.
The reuse of reclaimed wastewater for irrigation of agricultural fields greatly influences the activity of soil microorganisms through the input of organic compounds. Due to the production of exoenzymes by microorganisms for the decomposition of substrates it can be assumed that the irrigation with treated wastewater (TWW) has a strong influence on the soil enzyme pool. In this study the activity of ten exoenzymes, which catalyses processes in C, N and P nutrient cycles, were determined in 3 different soils in 0-10, 10-20, 20-30, 30-50, 50-70 and 70-100 cm soil depth. The soils were used for agriculture and irrigated with reclaimed wastewater reused after a secondary treatment step. Additionally a control after freshwater irrigation was studied. Due to the...
Tipo: Presentation Palavras-chave: Ecology; Microbiology; Earth & Environment.
Ano: 2011 URL: http://precedings.nature.com/documents/6278/version/1
Imagem não selecionada

Imprime registro no formato completo
Aeromonas no processamento de queijos tipos Minas Frescal e Colonial. Repositório Alice
CERESER, N. D.; ROSSI JÚNIOR, O. D.; MARTINELI, T. M.; SOUZA, V. de; RODRIGUES, L. B.; CARDOZO, M. V..
Resumo: Com o objetivo de estabelecer, durante o processamento do queijo Minas Frescal e Colonial os possíveis pontos de contaminação e a forma de disseminação de bactérias do gênero Aeromonas, foram analisados, quanto à presença do micro-organismo, diferentes produtos e pontos do fluxograma de produção. Para o Queijo Minas Frescal, Aeromonas spp. foram isoladas no leite cru, leite pasteurizado, ambiente de produção e nas mãos dos manipuladores. A. caviae foi a espécie mais frequentemente identificada, sendo também isoladas A. sobria e A. schubertii. Durante o processamento do queijo Colonial, as espécies A. hydrophila, A. caviae, A. sobria, A. veronii e A. jandaei foram isoladas a partir da água, mãos dos manipuladores, utensílios, leite cru e após...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Queijo Minas Frescal; Queijo Colonial; Produção artesanal; Bacteria; Microrganismo; Contaminação bacteriana; Higiene de alimento; Microbiologia de alimento; Consumidor; Análise de risco; Aeromonas; Microbiology; Food contamination; Cheese; Risk analysis.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/973437
Imagem não selecionada

Imprime registro no formato completo
Aggregation of Single Nucleotide Polymorphisms in a Human H5N1 Clade 2.2 Hemagglutinin Nature Precedings
Henry L. Niman; Magdi D. Saad; Jeffery Tjaden; Kenneth C. Earhart; Marshall R. Monteville; Mona M. Aly; Moustafa M. Mansour; Nasr El-Sayed; Ahmed E. Nayel; Ahmed S. Abdelghani; Hala M. Esmat; Emad M. Labib; Ehab A. Ayoub; Abdelattar Arafa; Gregory A. Raczniak; Mensah Agyen-Frempong; William K. Ampofo; Bruce R. Boynton.
The evolution of H5N1 has attracted significant interest 1-4 due to linkages with avian 5,6 and human infections 7,8. The basic tenets of influenza genetics 9 attribute genetic drift to replication errors caused by a polymerase complex that lacks a proof reading function. However, recent analysis 10 of swine influenza genes identifies regions copied with absolute fidelity for more than 25 years. In addition, polymorphism tracing of clade 2.2 H5N1 single nucleotide polymorphisms identify concurrent acquisition 11 of the same polymorphism onto multiple genetic backgrounds in widely dispersed geographical locations. Here we show the aggregation of regional clade 2.2 polymorphisms from Germany, Egypt, and sub-Sahara Africa onto a human Nigerian H5N1...
Tipo: Manuscript Palavras-chave: Biotechnology; Ecology; Genetics & Genomics; Immunology; Microbiology; Bioinformatics.
Ano: 2007 URL: http://precedings.nature.com/documents/743/version/1
Imagem não selecionada

Imprime registro no formato completo
Allergies, Asthma, and Eczema: Response to Disturbance of the Microbiota of the Newborn Gut Nature Precedings
Debra L. Wohl; William J. Curry.
*Background/Question/Methods*
The birth of a child may include routine use of antibiotics or a delivery by cesarean section. We applaud these medical advances for their direct benefits. However, birthing practices have been shown to disrupt the colonization of the microbial gut in newborns, which may have long term consequences on the development of a child’s immune system resulting in atopies (i.e., asthma, allergies, and eczema). Animal models and other research have been used to demonstrate that not only the presence, but also the community composition of the gut microbiota, is essential for normal immune development. Furthermore, it has been established that there are differences in the intestinal microbiota of children...
Tipo: Presentation Palavras-chave: Ecology; Microbiology.
Ano: 2009 URL: http://precedings.nature.com/documents/3685/version/1
Registros recuperados: 389
Primeira ... 123456789 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional