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A NEW WORLD MONKEY MICROSATELLITE (AP74) HIGHLY CONSERVED IN PRIMATES Acta biol.Colomb.
OKLANDER,LUCIANA INÉS; STEINBERG3,ELIANA RUTH; DOLORES MUDRY,MARTA.
Given their great variability, microsatellites or STRs became the most commonly used genetic markers over the last 15 years. The analysis of these markers requires minimum quantities of DNA, allowing the use of non invasive samples, such as feces or hair. We amplified the microsatellite Ap74 in blood and hair samples in order to analyze the levels of genomic conservation among a wide range of primates including: Lemur catta, Alouatta caraya, Ateles belzebuth, Ateles chamek, Pan troglodytes, Papio sp., and Homo sapiens. In all cases we obtained amplification products that exhibited similar size both in monkeys and human (oscillating between 126 and 176 bp), except in the lemur where the detected fragment presented a size of approximately 1000 bp. The...
Tipo: Journal article Palavras-chave: Primates; Microsatellite markers; PCR amplification; Non-invasive sampling; Genomic conservation; Genetic variability.
Ano: 2012 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2012000100007
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Assessment of est-ssr markers for genetic analysis on coffee. Repositório Alice
MISSIO, R. F.; CAIXETA, E. T.; ZAMBOIM, E. M.; PENA, G. F.; RIBEIRO, A. P.; ZAMBOLIM, L.; PEREIRA, A. A.; SAKIYAMA, N. S..
2009
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: DNA markers; Coffea sp; UPGMA; AMOVA; Microsatellite markers.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/handle/doc/880408
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Assessment of genetic diversity in Brazilian barley using SSR markers. Repositório Alice
FERREIRA, J. R.; PEREIRA, J. F.; TURCHETTO, C.; MINELLA, E.; CONSOLI, L.; DELATORRE, C. A..
2016
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Domesticated barley; Genetic diversity; Hordeum vulgare ssp vulgare; Hordeum vulgare ssp spontaneum; Microsatellite markers; Cevada; Marcador molecular.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1042255
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Bayesian analysis of the genetic structure of a Brazilian popcorn germplasm using data from simple sequence repeats (SSR) Chilean J. Agric. Res.
Saavedra,Javier; Silva,Tereza Aparecida; Mora,Freddy; Scapim,Carlos Alberto.
Several studies have confirmed that popcorn (Zea mays L. var. everta) has a narrow genetic basis, which affects the quality of breeding programs. In this study, we present a genetic characterization of 420 individuals representing 28 popcorn populations from Brazilian germplasm banks. All individuals were genotyped using 11 microsatellite markers from the Maize Genetics and Genomics Database. A Bayesian clustering approach via Monte Carlo Markov chains was performed to examine the genetic differentiation (Fst values) among different clusters. The results indicate the existence of three distinct and strongly differentiated genetic groups (K = 3). Moreover, the Fst values (calculated among clusters) were significantly different according to Bayesian credible...
Tipo: Journal article Palavras-chave: Genetic differentiation; Microsatellite markers; Bayesian clustering; Monte Carlo Markov chains.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392013000200003
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Comparison of multiallelic distances for the quantification of genetic diversity in the papaya - doi: 10.4025/actasciagron.v33i1.11698 Agronomy
Ramos, Helaine Christine Cancela; Universidade Estadual do Norte Fluminense; Pereira, Messias Gonzaga; Universidade Estadual do Norte Fluminense Darcy Ribeiro; Gonçalves, Leandro Simões Azeredo; Universidade Estadual do Norte Fluminense; Amaral Júnior, Antonio Teixeira do; Universidade Estadual do Norte Fluminense; Scapim, Carlos Alberto; Universidade Estadual de Maringá.
This study aimed to compare multiallelic distances to quantify genetic diversity in papaya. We evaluated forty-three individuals in the S2 generation, from the backcross between F1 (Cariflora x SS783) and Cariflora, and four accessions from the UENF/Caliman Germplasm Bank. Genetic distances used were Smouse and Peakall (1999), Kosman and Leonard (2005) and weighted index. Clustering among genotypes was performed using the hierarchical unweighted pair-group method with arithmetic mean analysis (UPGMA) and projection of the distance on the bidimensional plan. A high correlation between genetic distances was observed; however, through UPGMA group analysis, the distance determined by the weighted index provided the complete separation of 52BC1S2-08, 52BC1S2-29...
Palavras-chave: Carica papaya L.; Microsatellite markers; Cultivar characterization; Cluster analysis Carica papaya L.; Microsatellite markers; Cultivar characterization; Cluster analysis.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/11698
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Determination of genetic variability of traditional varieties of Brazilian rice using microsatellite markers. Repositório Alice
BRONDANI, C.; BORBA, T. C. O.; RANGEL, P. H. N.; BRONDANI, R. P. V..
The rice (Oryza sativa) breeding program of the Rice and Bean research center of the Brazilian agricultural company Empresa Brasileira de Pesquisa Agropecuária (Embrapa) is well established and provides new cultivars every year to attend the demand for improved high yielding varieties with tolerance to biotic and abiotic stresses. However, the elite genitors used to compose new populations for selection are closely related, contributing to the yield plateau reached in the last 20 years. To overcome this limit, it is necessary to broaden the genetic basis of the cultivars using diverse germplasm such as wild relatives or traditional varieties, with the latter being more practical because they are more easily crossed with elite germplasm to accelerate the...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Arroz; Oryza sativa; Rice; Recurso genético; Microsatellite markers; Brasil.
Ano: 2006 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/214434
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Development, characterization and use of genomic SSR markers for assessment of genetic diversity in some Saudi date palm (Phoenix dactylifera L.) cultivars Electron. J. Biotechnol.
Al-Faifi,Sulieman A; Migdadi,Hussein M; Algamdi,Salem S; Altaf Khan,Mohammad; Ammar,Megahed H; Al-Obeed,Rashid S; I. Al-Thamra,Mohammad; EL-Harty,Ehab H; Jakse,Jerenj.
Background: The present study was undertaken towards the development of SSR markers and assessing genetic relationships among 32 date palm (Phoenix dactylifera L.) representing common cultivars grown in different geographical regions in Saudi Arabia. Results: Ninety-three novel simple sequence repeat markers were developed and screened for their ability to detect polymorphism in date palm. Around 71% of genomic SSRs were dinucleotide, 25% tri, 3% tetra and 1% penta nucleotide motives. Twenty-two primers generated a total of 91 alleles with a mean of 4.14 alleles per locus and 100% polymorphism percentage. A 0.595 average polymorphic information content and 0.662 primer discrimination power values were recorded. The expected and observed heterozygosities...
Tipo: Journal article Palavras-chave: Expected and observed heterozygosity; Microsatellite markers; Polymorphic information content; Polymorphism; Primer discrimination power.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300002
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Development of rice lines with gene introgression from the wild Oryza glumaepatula by the AB-QTL methodology. Repositório Alice
RANGEL, P. H. N.; BRONDANI, C.; RANGEL, P. N.; BRONDANI, R. P. V.; ZIMMERMANN, F. J. P..
Wild rice of the species Oryza glumaepatula is found Brazil and has been used to broaden the genetic basis of irrigated rice populations in Embrapa breeding programmes. The objective of this study was to demonstrate and discuss approaches used in the development of Oryza sativa lines containing genes transferred from Oryza glumaepatula, resulting in introgression lines with a broader genetic basis and high yield. First of all, genes were transferred from the wild species to cultivated rice by the AB-QTL methodology. Eighteen families were selected using QTL analysis and agronomical performance data. After the heterosis test, the families CNAi 9020 and CNAi 9024 were selected and submitted to microsatellite marker-assisted selection. Thirty-five lines were...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Arroz irrigado; Marcadores microssatélites; Linhagens; Produtividade de grãos; Irrigated rice; Microsatellite markers; Lines; Grain yield; Oryza sativa.
Ano: 2005 URL: http://www.alice.cnptia.embrapa.br/handle/doc/213573
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Diversidade genética de populações de andiroba no Baixo Acre Repositório Alice
RAPOSO, A.; MARTINS, K.; CIAMPI, A.Y.; WADT, L.H. de O.; VEASEY, E.A..
O objetivo deste trabalho foi avaliar a diversidade genética em duas populações de Carapa guianensis Aubl. (andiroba), no Estado do Acre, e comparar os parâmetros de diversidade com os observados em outras populações da espécie (no Brasil: Flona Tapajós, PA, Porto Acre, AC; e na Costa Rica). Foram avaliados 77 indivíduos adultos com sete locos polimórficos de microssatélites. Observaram-se 51 alelos nas duas populações, em que o número efetivo de alelos por loco (A^ e = 3,2) foi inferior ao número médio de alelos por loco (A^ = 7,3), o que indica elevado número de alelos com baixa freqüência. Os valores estimados de f^ não diferiram de zero, o que mostra que não ocorre endogamia nas populações. A taxa de cruzamento aparente foi alta (t^a = 1,11 na...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Carapa guianensis; Marcadores microssatélites; Tamanho efetivo populacional; Conservação de recursos genéticos; Alelos raros; Fluxo gênico; Microsatellite markers; Effective population size; Genetic resources conservation; Rare alleles; Gene flow.
Ano: 2007 URL: http://www.alice.cnptia.embrapa.br/handle/doc/122809
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Diversity and genetic structure of mangaba remnants in states of northeastern Brazil. Repositório Alice
AMORIM, J. A. E.; MATA, L. R.; LEDO, A. S.; AZEVEDO, V. C. R.; SILVA, A. V. C..
2015
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Genetic variability; Hancornia speciosa; Microsatellite markers; Species preservation.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1033213
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Evolutionary dynamics of Cucurbita argyrosperma from the Mesoamerican domestication center using SSR molecular markers. Repositório Alice
PRIORI, D.; VILLARREAL, D. Z.; CANCHÉ EK, V. M. de J.; LIMONES-BRIONES, V.; BARBIERI, R. L..
bitstream/item/177015/1/Rosa-Lia-evolutionary-dynamics-of-Cucurbita-argyrosperma.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Genetic diversity; Microsatellite markers; Squash; Cucurbitaceae; Plant genetic resources.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1091513
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Genetic charecterization of cotton landraces found in the Paraíba and Rio Grande do Norte states. Repositório Alice
MENEZES, I. P. P. DE; HOFFMANN, L. V.; BARROSO, P. A. V..
The objective of this study was to estimate genetic diversity of cotton mocó planted in Paraíba and Rio Grande do Norte using microsatellite markers, since mocó landraces are a valuable source of genetic diversity. A set of 38 accessions - 21 plants from Rio Grande do Norte and 17 from Paraiba - was analyzed using 24 pairs of cotton microsatellite primers, which amplified 20 polymorphic loci. The average inbreeding was 0.432, and was slightly higher in individuals from Paraíba than from Rio Grande do Norte. Genetic diversity (Nei´s unbiased estimator) between individuals from each state?s populations had similar values (HT = 0.327 and 0.302 in Paraíba and Rio Grande do Norte, respectively), indicating that comparable variability has been maintained....
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Cotton; Genetic structure; Microsatellite markers; Gossypium hirsutum.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1041495
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Genetic diversity and population structure of upland cotton Brazilian cultivars (Gossypium hirsutum L. raça latifolium H.) using SSR markers. Repositório Alice
MOIAMA, L. D.; VIDIGAL FILHO, P. S.; GONÇALVES-VIDIGAL, M. C.; CARVALHO, L. P. de.
To better understand the genetic diversity of the cultivated upland cotton (Gossypium hirsutum L.) and its structure at the molecular level, microsatellite markers were used. The objective of this study was to evaluate genetic diversity and population structure in tetraploid cotton (Gossypium hirsutum L. race latifolium H.). Twenty cultivars and inbred lines from Embrapa Cotton Breeding Program, Brazil, were analyzed. From a total of 33 microsatellite (SSR) markers, twenty seven markers revealed 91 polymorphic SSR alleles. Two sub-populations were identified applying different methods (The Bayesian analysis, Principal Coordinates Analysis and Neighbor Joining Tree). Most of the cultivars belongs to Embrapa Cotton Breeding Program were allocated in...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Microsatellite markers; Upland cotton; Polymorfic SSR alleles; Gossypium hirsutum; Algodão; Genótipo.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1041531
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Genetic Diversity and Structure of Ruzigrass Germplasm Collected in Africa and Brazil. Repositório Alice
PESSOA FILHO, M. A. C. de P.; AZEVEDO, A. L. S.; SOUZA SOBRINHO, F. de; GOUVEA, E. G.; MARTINS, A. M.; FERREIRA, M. E..
Results of this study indicated that the introduction of ruzigrass in Brazil did not cause a major decrease in genetic diversity. Brazilian local populations might harbor favorable alleles that will be useful for ongoing and future breeding programs.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Tropical forage; Brachiaria species; Microsatellite markers.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1035303
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Genetic diversity associated with agronomic traits using microsatellite markers in Pakistani rice landraces Electron. J. Biotechnol.
Pervaiz,Zahida Hassan; Rabbani,M. Ashiq; Khaliq,Ishtiaq; Pearce,Stephen R; Malik,Salman A.
Genetic diversity underlies the improvement of crops by plant breeding. Land races of rice (Oryza sativa L.) can contain some valuable alleles not common in modern germplasm. The aim here was to measure genetic diversity and its effect on agronomic traits among rice land-race genotypes grown in Pakistan. Diversity was measured using thirty-five microsatellite markers and seventy-five genotypes. Among the markers used a total of 142 alleles were detected at 32 polymorphic SSR loci, while three loci were monomorphic in Pakistani rice landraces. The number of alleles identified by each marker ranged from 2 to 13 with a mean of 4.4. Size differences between the smallest and largest alleles varied from 11bp to 71bp. Polymorphism information content ranged from...
Tipo: Journal article Palavras-chave: Genetic diversity; Landraces; Microsatellite markers; Oryza sativa L.; Pakistan; Rice.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300004
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Genetic diversity of arboreal cotton populations of the Brazilian semiarid: a remnant primary gene pool for cotton cultivars. Repositório Alice
MENEZES, I. P. P. DE; HOFFMANN, L. V.; SILVA, A. R. DA; BARROSO, P. A. V..
Mocó cotton belong to the same species as the cultivated species, Gossypium hirsutum, and cultivated forms were mainly landraces but also developed as cultivars, bearing good fiber quality and drought tolerant when cropped as a perennial species. The northeast Brazil crop system based on this cotton type is finished, with a few small area planted in the three main States, where it was previously cultivated (Ceará, Paraíba, and Rio Grande do Norte), but in others, maintenance is accomplished by single dooryard plants. Plants were found in all visited Northeast Brazil municipalities, sometimes in the North of the country, and were collected for ex situ preservation and evaluation. Most of seeds had no fuzz (62.2%) and 94.6% of the genotypes presented spot in...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Mocó cotton; Microsatellite markers; Genetic diversity; Germplasm conservation; Genetic structure.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1074553
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Genetic impact of the reproduction dynamics in the European flat oyster Ostrea edulis ArchiMer
Lapegue, Sylvie; Taris, Nicolas; Lallias, Delphine; Bonhomme, Francois; Boudry, Pierre.
The European flat oyster (Ostrea edulis L.) is a marine bivalve whose natural geographical distribution ranges along the European Atlantic coast from Norway to Morocco, in addition to the Mediterranean and Black Sea. The latest results obtained on the genetic differentiation between these populations have led us to persue studies at a finer scale, in order to estimate the effective number of breeders and the temporal dynamics of reproduction and, more specially, recruitment. Several experiments were performed to document (1) the variance in allele frequencies during a natural settlement period, (2) the paternal contribution to fertilization by analyzing larvae sampled at the brooding stage within individual females, (3) the variance of individual...
Tipo: Text Palavras-chave: Recruitment; Microsatellite markers; Ostrea edulis; European flat oyster; Genetic.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/acte-3485.pdf
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Genetic improvement of hatchery propagated bivalve stocks : prospects and constraints ArchiMer
Boudry, Pierre.
To date, the most significant genetic improvement for the production of Pacific oyster (Crassostrea gigas) has been obtained through the production of triploids, especially since the development of tetraploids. Quantitative genetics studies suggest that significant gains, for disease resistance or other traits, could be obtained in diploids. However, the limited extent of hatchery-propagation (versus natural recruitment) and/or various technical difficulties and biological characteristics of the species have retarded the development of selective breeding programs. In the U.S.A., Australia and New Zealand, family-based selective breeding programs have recently been initiated to improve growth, disease tolerance and yield. In Europe, where both natural and...
Tipo: Text Palavras-chave: Microsatellite markers; Crassostrea gigas; Selective breeding; Triploid; Pacific oyster; Genetic.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3451.pdf
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Genetic relationships between Cuban and Brazilian buffaloes (Bubalus bubalis) by microsatellite markers Rev Salud Anim.
Martínez Marrero,Nadia; da Silva,Elizabete Cristina; Mitat Valdés,Alina; Ponce,Giselle; Benício da Silva,Regina C.; Paes Barbosa,Severino Benone; Uffo Reinosa,Odalys; Gomes Filho,Manoel Adrião.
Researches on genetic relationships among water buffalo populations are useful in supporting conservation decisions and designing of breeding schemes. The aim of this study was to assess the genetic relationships among Cuban Buffalypso/Carabao hybrids and Murrah and Jaffarabadi Brazilian buffaloes using nine cattle microsatellite markers. During the analysis, four loci were excluded due to the presence of null alleles, stuttering and gametic disequilibrium. Mean numbers of alleles and the heterozygosities observed and expected in the three populations were not significantly different. Heterozygosities found were above 60%. All five loci were in Hardy-Weinberg equilibrium. Wright´s index for estimating inbreeding were negative in the three populations. A...
Tipo: Journal article Palavras-chave: Bubalus bubalis; Buffalypso; Microsatellite markers; Null allele; Genetic relationships.
Ano: 2015 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2015000300003
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Genetic variability analysis of elite upland rice genotypes with SSR markers. Repositório Alice
BRONDANI, C.; CALDEIRA, K. da S.; BORBA, T. C. O.; RANGEL. P. N.; MORAIS, O. P. de; CASTRO, E. da M. de; RANGEL, P. H. N.; MENDONÇA, J. A.; BRONDANI, R. V..
Rice is one of the staple foods consumed worldwide, and rice breeding programmes have become important to warrant high yield levels and grain quality in upland rice. Laboratory experiments were conducted to study the genetic variation of 30 elite genotypes from a VCU trial, of the upland rice breeding programme of Embrapa, Brazil, using 25 SSR markers. A total of 131 alleles were obtained, with an average of 5.2 alleles per locus and a mean PIC equal to 0.61. Results indicated that genetically different elite parents from the breeding programme and selection in segregating families have given rise to broad-based rice genotypes. Based on the analysis of different combinations of 10 SSR markers, it was observed that the use of more informative markers is...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Arroz; Oryza sativa; Rice; Recurso genético; Marcador microssatélite; Distância genética; Genetic Resources; Microsatellite markers; Genetic distance.
Ano: 2006 URL: http://www.alice.cnptia.embrapa.br/handle/doc/214369
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