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Glutenin analysis for the safe screening of self-pollinated wheat seeds when developing doubled-haploid populations PAB
Torres,Gisele Abigail Montan; Scagliusi,Sandra Maria Mansur; Chaves,Márcia Soares; Consoli,Luciano.
Abstract: The objective of this work was to apply glutenin analysis, as a simple and reliable method, to identify self-pollinated wheat (Triticum aestivum) seeds in F1 crosses, when developing mapping populations using doubled-haploid (DH) technology. F1 seeds were subjected to glutenin analyses using gel electrophoresis, to verify and confirm their heterozygotic profiles. Glutenin analyses were carried out using one half of the endosperm of each of the 87 F1 seeds. The other half of the endosperm was used to develop the mapping population through DHs. Plants with band patterns different from the expected ones were discarded. Although the incidence of self-pollinated seeds was relatively low (only 8%), the screening of F1 heterozygotic profiles is...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Puccinia recondita f. sp. tritici; Triticum aestivum; Adult plant resistance; Leaf rust; Microspore culture; Toropi wheat cultivar.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000500646
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Induction of symmetrical nucleus division and multi-nucleate structures in microspores of eggplant (Solanum melongena L.) cultured in vitro Scientia Agricola
Bal,Ugur; Ellialtioglu,Sebnem; Abak,Kazim.
A modification of a protocol used to induce tobacco microspore embryogenesis was tested in eggplant (Solanum melongena L.). In tobacco, uninucleate microspores are subjected to stress treatment by culturing in mannitol containing "B" medium at 33ºC for six days. The microspores are then transferred to maltose containing AT3 medium for further development. In the experiment presented here late uninucleate and bi-nucleate microspores of the eggplant cultivar Bambino were pre-cultured in B medium and then incubated at +4ºC, 25ºC and 33ºC, respectively, for two days. After the pre-treatments, microspore cultures were transferred to AT3 medium containing 0.25 M maltose and maintained at 25ºC in the dark. Presence of symmetrical division and multinucleate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Haploid; Microspore culture; Symmetrical division; Mannitol starvation.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000400016
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Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains BABT
Rodrigues,Lia Rosane; Forte,Bianca de Camargo; Bodanese-Zanettini,Maria Helena.
In the last three decades, research on soybean microspore embryogenesis was restricted to anther culture, which presents limitations such as the small number of responsive microspores and the high embryogenic potential of sporophytic tissues. Therefore, a sequence of studies was performed to establish appropriate conditions for the isolation and culture of soybean microspores and pollen grains as an alternative to anther culture. First, a pollen and microspore isolation technique was developed using floral buds from four soybean cultivars (Bragg, IAS 5, MG/BR-46 Conquista and BRSMT Uirapuru). This technique allowed the establishment of cultures with satisfactory density and characteristics. Subsequently, different culture conditions were tested. Although...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Androgenesis; Glycine max; Microspore culture; Microspore embryogenesis; Microspore isolation; Soybean.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500002
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