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Registros recuperados: 28
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Comparative phylogeography of the western Indian Ocean reef fauna ArchiMer
Borsa, Philippe; Durand, Jean-dominique; Chen, Wei-jen; Hubert, Nicolas; Muths, Delphine; Mou-tham, Gerard; Kulbicki, Michel.
Assessing patterns of connectivity at the community and population levels is relevant to marine resource management and conservation. The present study reviews this issue with a focus on the western Indian Ocean (WIO) biogeographic province. This part of the Indian Ocean holds more species than expected from current models of global reef fish species richness. In this study, checklists of reef fish species were examined to determine levels of endemism in each of 10 biogeographic provinces of the Indian Ocean. Results showed that the number of endemic species was higher in the WIO than in any other region of the Indian Ocean. Endemic species from the WIO on the average had a larger body size than elsewhere in the tropical Indian Ocean. This suggests an...
Tipo: Text Palavras-chave: Indo-West Pacific; Biogeographic province; Endemism; Reef fish; Mitochondrial DNA; Parsimony network.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00288/39919/44195.pdf
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Diversité et différentiation génétiques des populations de tortues vertes (Chelonia mydas) dans les sites de ponte et d'alimentation du sud-ouest de l'océan Indien : application aux stratégies de conservation de l'espèce ArchiMer
Taquet, Coralie.
The green turtle (Chelonia mydas) is an emblematic species of marine life. However, nowadays it is subject to many threats (poaching, by-catch). Even if there is deep growing measures for its protection, the green turtle still is an endangered species and it is listed in Appendix I of Washington Convention (CITES). In order to elaborate efficient conservation and management plans, perfect knowledge of green turtle biology, but also of its population structure and their characteristics, are needed. In this thesis, we have assessed genetic structure of green turtle populations in the South-Western Indian Ocean by using genetic tools. In all, 1551 tissue samples have been collected from our study zone and from our control site French Polynesia (37 samples)....
Tipo: Text Palavras-chave: Management units; Microsatellites; Mitochondrial DNA; Foraging sites; Nesting sites; South Western Indian Ocean; Population genetics; Chelonia mydas; Green turtle; Unités de gestion (MUs); Microsatellites; ADN mitochondrial; Sites d'alimentation; Sites de ponte; Génétique des populations; Chelonia mydas; Tortue verte.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/these-3532.pdf
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D-loop haplotype diversity in Brazilian horse breeds. Repositório Alice
IANELLA, P.; ALBUQUERQUE, M. do S. M.; PAIVA, S. R.; EGITO, A. A. do; ALMEIDA, L. D.; SERENO, F. T. P. S.; CARVALHO, L. F. R.; MARIANTE, A. da S.; MCMANUS, C. M..
bitstream/item/165902/1/1415-4757-gmb-1678-4685-GMB-2016-01661.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Genetic characterization; Locally adapted breeds; Mitochondrial DNA; Equus caballus; Animal genetic resources.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1078580
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Genealogy of the nuclear β-fibrinogen intron 7 in Lissotriton boscai (Caudata, Salamandridae): concordance with mtDNA and implications for phylogeography and speciation Naturalis
Teixeira, J.; Martínez-Solano, I.; Buckley, D.; Tarroso, P.; García-París, M.; Ferrand, N..
The power of phylogeographic inference resides in its ability to integrate information from multiple sources in an iterative hypothesis-testing framework. In this paper, we build upon previous mtDNA-based hypotheses about the evolutionary history of the Iberian newt Lissotriton boscai using sequences of the highly variable nuclear β-fibrinogen intron 7. In addition to the nuclear sequences, we produced new mtDNA data across the species range to delineate contact zones and test the congruence between nuclear and mitochondrial datasets at the same level of spatial organization. Through a combination of phylogenetic, phylogeographic continuous diffusion, and genetic landscape modelling analyses, we infer the evolutionary history of the species. We found...
Tipo: Article / Letter to the editor Palavras-chave: Β-fibint7; Genetic landscape shape; Iberian Peninsula; Mitochondrial DNA; Nuclear DNA; 42.82; 42.64.
Ano: 2015 URL: http://www.repository.naturalis.nl/record/578951
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Genetic origin of Brazilian local adapted sheep (Ovis aries) breeds by complete mitochondrial genome data analysis. Repositório Alice
LOBO, F. P.; YAMAGISHI, M. E. B.; CAETANO, A. R.; MCMANUS, C. M.; CARNEIRO, P. L.; FACO, O.; SOUZA, C. J. H.; PAIVA, S. R..
The origin and domestication footprints present in contemporary domestic sheep breeds are of fundamental importance to provide insights regarding the identification of major genetic pools and breeds of importance for conservation. To infer the origin of locally adapted Brazilian sheep, the complete mitochondrial sequence of six animals from three main breeds (Morada Nova, Santa Ines and Brazilian Creole) were assembled from data generated by the International Sheep Genome Consortium. Paired-end DNA sequences (100bp) were generated using a HiSeq2000 platform from shotgun libraries to a depth of genome coverage averaging 12X. mtDNA sequences were identified, aligned and assembled with the Burrows-Wheeler Alignment Tool, using NC_001941 as reference (coverage...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Ovelha; Genômica; Sequência de DNA; Sheep; Genomics; Mitochondrial DNA.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/974765
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Genetic Relationships of Common Bean (Phaseolus vulgaris L.) Race Chile with Wild Andean and Mesoamerican Germplasm Chilean J. Agric. Res.
Becerra V,Viviana; Paredes C,Mario; Debouck,Daniel.
The Chilean common bean (Phaseolus vulgaris L.) belongs to the cultivated race Chile and its origin is presumably Andean. The objective of this study was to identify the origin of a group of Chilean accessions based on their genetic relationship with wild material from the Mesoamerican and Andean common bean gene pool. To achieve this objective, universal primers of chloroplast DNA (cpDNA) and mitochondrial DNA (mtDNA) were used to detect polymorphism using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP). Thirty-two genotypes were analyzed, including wild material from Mexico, Ecuador, Peru, Bolivia, and Argentina, as well as Chilean cultivated genotypes belonging to endemic Chilean accession types (Tórtola, Coscorrón, and...
Tipo: Journal article Palavras-chave: Chilean common bean germplasm; Chloroplast DNA; Mitochondrial DNA; PCR-RFLP.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000100001
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GENETIC STATUS, SOURCE AND ESTABLISHMENT RISK OF THE GIANT TIGER SHRIMP (PENAEIDAE: Penaeus monodon), AN INVASIVE SPECIES IN COLOMBIAN CARIBBEAN WATERS Acta biol.Colomb.
AGUIRRE-PABÓN,J.C; OROZCO BERDUGO,G. Jr; NARVÁEZ BARANDICA,J.C.
The tiger shrimp (Penaeus monodon) is an Indo-Pacific species. Its global production between 1970 and 1980 exceeded all other shrimp species, which favored its introduction and cultivation outside its natural range in several countries of Africa, Europe, USA and South America. It is currently found in the coast of the Atlantic Ocean (Mexico, United States, Puerto Rico, Brazil, Guyana, Venezuela and Colombia). Despite the risk involved, no studies have been conducted to evaluate their impact as a possible invasive species and their genetic condition. This study evaluated the genetic status and population origin of P. monodon in the northernmost Colombian Caribbean, analyzing the mitochondrial DNA control region (mtDNA-CR). 16 individuals were randomly...
Tipo: Journal article Palavras-chave: Biological invasion; Caribbean Sea; Founder effect; Genetic relationships; Mitochondrial DNA.
Ano: 2015 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2015000100013
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Genetic structure of the ornamental tetra fish species Piabucus melanostomus Holmberg, 1891 (CHARACIDAE, IGUANODECTINAE) in the Brazilian Pantanal wetlands inferred by mitochondrial DNA sequences. Repositório Alice
BORBA, R. S. de; SILVA, E. L. da; PONZETTO, J. M.; POZZOBON, A. P. B.; CENTOFANTE, L.; ALVES, A. L.; PARISE-MALTEMPI, P. P..
The subfamily Iguanodectinae comprises a group of small Neotropical fishes composed by two genera and 11 nominal species widely distributed in the Atlantic drainages of South America. Piabucus is the only genus of Iguanodectinae found in the Paraguay River basin, especially in the Pantanal of Mato Grosso State, where it is represented by Piabucus melanostomus. Given the wide distribution and the low dispersion capacity of this species, due the ecological constraints, it is possible that many interesting genetic features could be found in different populations. In this way, the aim of his work was to perform the phylogeographic pattern of P. melanostomus populations using mitochondrial DNA sequences. A total of 13 individuals from three rivers belonging the...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Pantanal; Peixe ornamental; Genética; Mitocondria; Ornamental fish; Genetics; Mitochondrial DNA.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/948641
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Genetic variability of the Amazon River prawn Macrobrachium amazonicum (Decapoda, Caridea, Palaemonidae) Naturalis
Vergamini, F.G.; Pileggi, L.G.; Mantelatto, F.L..
The freshwater prawn Macrobrachium amazonicum is widely distributed in South America, and occupies habitats with a wide range of salinities. Several investigations have revealed the existence of wide intraspecific variability among different populations, although the understanding of this variability is still fragmentary and incomplete. We compared and characterized inland and coastal populations of M. amazonicum from Brazil, using molecular data (16S and COI mtDNA) to describe the degree of variability, structure, and relationships among them. Genetic divergence rates among populations showed variability at the intraspecific level. All the analyses evidenced significant genetic divergence among populations, structuring them in three groups: I- inland...
Tipo: Article / Letter to the editor Palavras-chave: Brazil; Genetic distance; Inland versus coastal populations; Phylogeny; Mitochondrial DNA; Population; 42.74.
Ano: 2011 URL: http://www.repository.naturalis.nl/record/369711
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Hierarchical analysis of mtDNA variation and the use of mtDNA for isopod (Crustacea: Peracarida: Isopoda) systematics Naturalis
Wetzer, R..
Carefully collected molecular data and rigorous analyses are revolutionizing today’s phylogenetic studies. Although molecular data have been used to estimate various invertebrate phylogenies lor more than a decade, this study is the first survey of different regions of mitochondrial DNA in isopod crustaceans assessing sequence divergence and hence the usefulness of these regions to infer phylogeny at different hierarchical levels. 1 evaluate three loci from the mitochondrial genome (two ribosomal RNAs (12S, 16S) and one protein-coding (COI)) for their appropriateness in inferring isopod phylogeny at the suborder level and below. The patterns are similar for all three loci with the most speciose suborders of isopods also having the most divergent...
Tipo: Article / Letter to the editor Palavras-chave: Mitochondrial DNA; Isopod; Crustacea; Molecular; 12S rRNA; 16S rRNA; COI.
Ano: 2001 URL: http://www.repository.naturalis.nl/record/534397
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High passage numbers induce resistance to apoptosis in C2C12 muscle cells Biocell
Pronsato,Lucia; La Colla,Anabela; Ronda,Ana Carolina; Milanesi,Lorena; Boland,Ricardo; Vasconsuelo,Andrea.
Cell lines with high passage numbers exhibit alterations in cell morphology and functions. In the present work, C2C12 skeletal muscle cells with either low (<20) or high (&gt;60) passage numbers (identified as l-C2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2O2 as a function of culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA (mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential, we observed that cells in the h-C2C12 group were resistant to H2O2 induction of apoptosis. We propose serially cultured C2C12 cells as a refractory model to H2O2-induced apoptosis. In addition, the data obtained in this work suggest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Over-subculturing changes; Aged muscle cultures; Mitochondrial DNA.
Ano: 2013 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452013000100001
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Honey bees (Hymenoptera: Apidae) of african origin exist in non-africanized areas of the Southern United States: evidence from mitochondrial DNA IPB - Escola Superior Agrária
Pinto, M. Alice; Sheppard, Walter S.; Johnston, J. Spencer; Rubink, William L.; Coulson, Robert N.; Schiff, Nathan M.; Kandemir, Irfan; Patton, John C..
Descendents of Apis mellifera scutellata Lepeletier (Hymenoptera: Apidae) (the Africanized honey bee) arrived in the United States in 1990. Whether this was the Þrst introduction is uncertain. A survey of feral honey bees from non-Africanized areas of the southern United States revealed three colonies (from Georgia, Texas, and New Mexico) with a diagnostic African mitochondrial DNA cytochrome b/BglII fragment pattern. To assess maternal origin of these colonies, we developed a primer pair for ampliÞcation of a cytochrome b fragment and sequenced using internal sequencing primers. Samples of the three reported honey bee colonies plus another 42 representing the 10 subspecies known to have been introduced in the United States were sequenced. Of the three...
Tipo: Article Palavras-chave: Africanized honey bee; Apis mellifera; Mitochondrial DNA; Cytochrome b; Mitotype.
Ano: 2007 URL: http://hdl.handle.net/10198/2891
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Identificação molecular de Cichla (Perciformes: Cichlidae) introduzidas nos reservatórios do Sul do Brasil. Molecular identification of Cichla (Perciformes: Cichlidae) introduced in the reservoirs of the South Region in Brazil Biological Sciences
Briñez, Boris; Universidade Estadual de Maringá; Júlio Júnior, Horacio Ferreira; Universidade Estadual de Maringá; Prioli, Sonia Maria Alves Pinto; Universidade Estadual de Maringá; Maniglia, Thiago Cintra; Núcleo de Pesquisas em Limnologia, Ictiologia e Aqüicultura (Nupelia), Universidade Estadual de Maringá; Prioli, Alberto José; Universidade Estadual de Maringá.
Espécies de tucunaré foramn introduzidas em inúmeras bacias hidrográficas da América do Sul e em outras regiões do planeta, principalmente devido as suas características esportivas, de peixe lutador. Revisão recente das espécies do gênero Cichla mostraram que as espécies que foram introduzidas nos reservatórios das regiões Sul, Sudeste e Nordeste, são duas espécies novas, descritas como Cichla kelberi (tucunaré amarelo) e Cichla piquiti (tucunaré azul) identificadas erroneamente como C. monoculus e C. ocellaris. Com o objetivo de identificar as populações de Cichla presentes no Rio Paranapanema e Paraná, foram sequenciadas um total de 323 pares de bases (pb) da região controle (MtDNA) obtidas de oitenta e quatro indivíduos de Cichla em cinco localidades...
Palavras-chave: 2.02.02.00-8 species introduction; C. monoculus; Mitochondrial DNA; Peacock bass; Paraná river Genética Species introduction C.monoculus MtDNA control region Peacock bass Upper Paraná River Tucunaré. Introdução de espécies C.monoculus região controle (MtDNA) Tucunaré Genética.
Ano: 2013 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/10684
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Identificación molecular de heces y análisis de hábitos alimenticios de carnívoros en la reserva de la biósfera "Sierra del Abra Tanchipa", San Luis Potosí, México. Colegio de Postgraduados
Benítez Alemán, Héctor Eduardo.
La identificación precisa de heces de carnívoros silvestres es importante para describir sus hábitos alimenticios, sobre todo en aquellas especies elusivas o en riesgo. Sin embargo Identificar las heces de especies de carnívoros simpátricos no es una tarea sencilla. Por ello, las técnicas moleculares, son herramientas que brindan mayor precisión en la identificación de heces. El análisis de hábitos alimenticios aporta información valiosa para entender la estructura poblacional de los depredadores y sus presas, así como su importancia ecológica para generar estrategias de manejo y conservación de carnívoros y sus presas. Los objetivos de este estudio fueron identificar las heces de carnívoros extrayendo ADN mitocondrial de estas y amplificando un fragmento...
Palavras-chave: ADN mitocrondrial; Citocromo b; PCR-RFLP; Hábitos alimenticios; Carnívoros; Mitochondrial DNA; Cytochrome b; Food habits; Carnivores; Ganadería; Maestría.
Ano: 2014 URL: http://hdl.handle.net/10521/2291
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Identification of africanized honey bee (Hymenoptera: apidae) mitochondrial DNA: validation of a rapid polymerase chain reaction-based assay IPB - Escola Superior Agrária
Pinto, M. Alice; Johnston, J. Spencer; Rubink, William L.; Coulson, Robert N.; Patton, John C.; Sheppard, Walter S..
Polymerase chain reaction (PCR)-ampliÞed mitochondrialDNA(mtDNA)assays have been used in studies of the Africanization process in neotropical feral and managed honey bee populations. The approach has been adopted, in conjunction with morphometric analysis, to identify Africanized bees for regulatory purposes in the United States such as in California. In this study, 211 Old World colonies, representing all known introduced subspecies in the United States, and 451 colonies from non-Africanized areas of the southern United States were screened to validate a rapid PCR-based assay for identiÞcation of Africanized honey bee mtDNA. This PCR-based assay requires a single enzyme digestion (BglII) of a single PCR-ampliÞed segment of the cytochrome b gene. The BglII...
Tipo: Article Palavras-chave: Africanized honey bee; A. m. scutellata; Mitochondrial DNA; MtDNA haplotype; Mitotype.
Ano: 2003 URL: http://hdl.handle.net/10198/2902
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Lineage Classification of Canine Inheritable Disorders Using Mitochondrial DNA Haplotypes OAK
TAKAHASI, Shoko; MIYAHARA, Kazuro; ISHIKAWA, Hirosi; ISHIGURO, Naotaka; SUZUKI, Mitsuyoshi; 宮原, 和郎; 鈴木, 三義.
To estimate the maternal effects of dog breeds using mitochondrial DNA(mtDNA) haplotypes in the dogs with several clinical disorders, 600 base pairs of mtDNA D-loop region were amplified from 365 dogs and were determined for mtDNA sequences. The diversity of the 600-bp sequences was classified into 64 haplotypes, including 46 newly discovered haplotypes, and the haplotypes were grouped into four clusters I to IV. Lineage analysis using the mtDNA haplotype indicated that each dog breed genetically comprises one or a few mtDNA haplotypes. When the relationship between genetic background and occurrences of clinical diseases was estimated, canine lineage analysis using mtDNA haplotype revealed that the disorders distributed in the dominant mtDNA haplotypes of...
Palavras-chave: D-loop; Haplotype lineage analysis; Mitochondrial DNA.
Ano: 2002 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4066
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Mitochondrial and nuclear DNA phylogeography of two cupped oysters Crassostrea gigas and Crassostrea angulata ArchiMer
Boudry, Pierre; Huvet, Arnaud.
The taxonomic status of Crassostrea angulata and Crassostrea gigas has long been a matter of controversy. Morphological and physiological similarities, as well as homogeneity in allelic frequencies on allozymes between the populations of the two taxa, lead most authors to suggest to regroup of the two within the same species. European and Asian populations of C. gigas and C. angulata have been studied using microsatellite and mitochondrial DNA markers. The analysis of genetic distances and the distribution of allelic and haplotype frequencies revealed a differentiation between the populations of C. gigas and C. angulata. The data allowed the construction of Neighbor-joining trees for each of the two types of markers. Similar topologies appeared with data...
Tipo: Text Palavras-chave: Mitochondrial DNA; Nuclear DNA; Phylogeography; Crassostrea angulata; Crassostrea gigas; Genetic; Oyster.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/acte-3434.pdf
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Mitochondrial Genetic Characterization Of Bluefin Tuna (Thunnus Thynnus) From Three Mediterranean (Libya, Malta, Tunisia); And One Atlantic Locations (Gulf Of Cadiz). OceanDocs
Farrugia, A.F.; Pla, C.; Viñas, J.; Tawil, M.Y.; Hattour, A.; de la Serna, J.M..
None of the previous genetic analysis found significant differences within the Mediterranean. However, a more exhaustive analysis is needed inside this sea. In this sense, we have analyzed the mitochondrial control region sequence variability of three Mediterranean locations: Libya (n = 22), Tunisia (n = 23) and Malta (n = 12). Additionally a sample from the Gulf of Cadiz (n = 24) in the East Atlantic Ocean was also included in the study. The AMOVA analysis revealed slightly (Fst = 0.013) but significative genetic differences (P < 0.000) for the Malta location, probably due to its small sample size (n = 12). In the other hand, when this location is removed from the analysis, no differences where found among the three remaining location (Fst = 0,005; P =...
Tipo: Journal Contribution Palavras-chave: Atlantic ocean; Mitochondrial DNA; Bluefin tuna; Sequencing; Genetic characterization; Mediterranean sea; Tuna fisheries.
Ano: 2003 URL: http://hdl.handle.net/1834/792
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Molecular phylogenetics and comparative anatomy of Kimberleytrachia Köhler, 2011 - a genus of land snail endemic to the coastal Kimberley, Western Australia with description of new taxa (Gastropoda, Camaenidae) Naturalis
Criscione, F.; Köhler, F..
Kimberleytrachia Köhler, 2011 is a genus of camaenid land snail endemic to the Western Australian Kimberley region. It comprises twelve previously recognised species, all of which occur within the high precipitation zone along the north-western coast between the Admiralty Gulf and King Sound and within less than about 50 km distance from the coast. By evaluating the variation in shell and genital anatomy as well as the differentiation in the mitochondrial markers 16S and COI, we assess the monophyly of Kimberleytrachia with respect to other camaenid genera from north-western Australia. In addition, we newly describe six species (K. jacksonensis n. sp., K. leopardus n. sp., K. nelsonensis n. sp., K. serrata n. sp., K. setosa n. sp. and K. silvaepluvialis n....
Tipo: Article / Letter to the editor Palavras-chave: Genetic distances; Helicoidea; Mitochondrial DNA; New species; Stylommatophora; 42.73; 42.64.
Ano: 2014 URL: http://www.repository.naturalis.nl/record/506810
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Morphological and genetic differentiation of Bufo toads: two cryptic species in Western Europe (Anura, Bufonidae) Naturalis
Arntzen, J.W.; McAtear, J.; Recuero, E.; Ziermann, J.M.; Ohler, A.; Alphen, J. van; Martínez-Solano, I..
The Common toad Bufo bufo sensu lato is a widespread, morphologically conserved taxon. Recent studies have uncovered deep genetic differentiation between population groups, highlighting the need to revise the current taxonomy of the group and recognize additional species. Here we investigate patterns of variation in molecular (a mitochondrial DNA restriction enzyme assay and sequence data for two nuclear DNA fragments totalling 979 bp) and 17 morphological variables in Northern France where two of these groups meet (B. bufo sensu stricto and B. spinosus), in order to delineate their contact zone and uncover characters that would allow discrimination of the two taxa. Mitochondrial DNA data show an abrupt transition from areas where B. bufo is present to...
Tipo: Article / Letter to the editor Palavras-chave: Bufo bufo; Bufo spinosus; Common toad; Contact zone; France; Mitochondrial DNA; Morphometrics; Nuclear DNA; 42.82.
Ano: 2013 URL: http://www.repository.naturalis.nl/record/475530
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