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A biologia avançada e o impacto da genômica na produção de caprinos e ovinos. Infoteca-e
SIDER, L. H.; ZAROS, L. G..
bitstream/CNPC-2010/21844/1/doc78.pdf
Tipo: Documentos (INFOTECA-E) Palavras-chave: Genômica; Caprino; Ovino; Biologia molecular; Goats; Sheep; Molecular biology; Genomics.
Ano: 2008 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/534121
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An overview of a diagnostic and epidemiologic reappraisal of cutaneous leishmaniasis in Iran BJID
Farahmand,Mahin; Nahrevanian,Hossein; Shirazi,Hasti Atashi; Naeimi,Sabah; Farzanehnejad,Zahra.
Cutaneous leishmaniasis (CL) is a widespread tropical infection which has a high incidence rate in Iran. Leishmania tropica, the causative agent of anthroponotic cutaneous leishmaniasis (ACL), and Leishmania major, which causes zoonotic cutaneous leishmaniasis (ZCL), are endemic in various parts of Iran with a high incidence rate. The aim of this study was to evaluate the reappraisal of the diagnosis and epidemiology of CL in Iran, by different clinical, parasitological and molecular assays among patients suspected of CL referred to the Department of Parasitology, at the Pasteur Institute of Iran during 2006-2009. Two hundred samples from patients with ulcerative skin lesions were collected, clinical analyses were applied, data questionnaire was completed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Parasitological analysis; Molecular biology; Leishmaniasis; Cutaneous; Iran; Epidemiology.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000100004
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Aplicação da biologia molecular em programas de conservação de recursos pesqueiros. Infoteca-e
MARQUES, D. K. S..
Estudos genéticos em peixes; Marcadores moleculares; Eletroforese de proteínas; Seqüências de DNA como marcadores; Polimorfismo de comprimento de fragmentos de restrição (Restriction Fragment Length Polymorphism - RFLP); Polimorfismo de DNA amplificado ao acaso (Randomly Aplified polymorphic DNA - RAPD); DNA com seqüências repetitivas: micro-satélites e mini-satélites; DNA mitocondrial.
Tipo: Documentos (INFOTECA-E) Palavras-chave: Marcadores moleculares; Recursos pesqueiros; Fisheries resources; Conservation.; Biologia Molecular; Conservação; Peixe; População.; Fish; Molecular biology; Population..
Ano: 2002 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/810724
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Applications of nucleoside-based molecular probes for the in vivo assessment of tumour biochemistry using positron emission tomography (PET) BABT
Wiebe,Leonard I..
Positron emission tomography (PET) is a non-invasive nuclear imaging technique. In PET, radiolabelled molecules decay by positron emission. The gamma rays resulting from positron annihilation are detected in coincidence and mapped to produce three dimensional images of radiotracer distribution in the body. Molecular imaging with PET refers to the use of positron-emitting biomolecules that are highly specific substrates for target enzymes, transport proteins or receptor proteins. Molecular imaging with PET produces spatial and temporal maps of the target-related processes. Molecular imaging is an important analytical tool in diagnostic medical imaging, therapy monitoring and the development of new drugs. Molecular imaging has its roots in molecular biology....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular biology; Molecular imaging; PET; Nucleosides; Nucleotides; Nucleic acids; Oligonucleotides; Antisense; Aptamer; Spiegelmer.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300011
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Assessment of the scientific-technological production in molecular biology in Brazil (1996-2007): the contribution of genomics programs Anais da ABC (AABC)
Meneghini,Rogério; Gamba,Estêvão C..
Several genome sequencing programs were launched in Brazil by the end of the nineties and the early 2000s.The most important initiatives were supported by the ONSA program (http://watson.fapesp.br/onsa/Genoma3.htm) and aimed at gaining domain in genomic technology and bringing molecular biology to the state of art. Two mainsets of data were collected in the 1996-2007 period to evaluate the results of these genome programs: the scientific production (Scopus and Web of Science databases) and the register of patents (US Patent and Trademark Office), both related to the progress of molecular biology along this period. In regard to the former, Brazil took a great leap in comparison to 17 other developed and developing countries, being only surpassed by China....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Science assessment; Genomics; Scientometrics; Molecular biology.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652011000200031
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Biologia molecular aplicada à diagnose de doenças de plantas. Infoteca-e
GONCALVES, R. C..
As doenças das plantas influenciam negativamente e de modo significativo as atividades agropecuárias e florestais em todo o mundo. Muitas epidemias tiveram como conseqüência grandes perdas econômicas, alterações sociais e ambientais.
Tipo: Capítulo em livro técnico (INFOTECA-E) Palavras-chave: Marcadores genéticos.; Enfermedades y desórdenes de las plantas; Doença de planta; Marcador genético; Biologia Molecular.; Plant diseases and disorders; Molecular biology; Genetic markers..
Ano: 2009 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/661798
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Biologia molecular aplicada à sanidade. Infoteca-e
SIDER, L. H..
bitstream/item/48454/1/Midia-Biologia-molecular.pdf
Tipo: Artigo de divulgação na mídia (INFOTECA-E) Palavras-chave: Biologia molecuar; Sanidade; Molecular biology.
Ano: 2009 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/577884
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Carbapenem and cefoxitin resistance of Klebsiella pneumoniae strains associated with porin OmpK36 loss and DHA-1 β-lactamase production BJM
Shi,Weifeng; Li,Kun; Ji,Yun; Jiang,Qinbo; Wang,Yuyue; Shi,Mei; Mi,Zuhuang.
Clinical isolates of carbapenem-resistant Klebsiella pneumoniae (K. pneumoniae) strains are being increased worldwide. Five pan-resistant K. pneumoniae strains have been isolated from respiratory and ICU wards in a Chinese hospital, and reveal strong resistance to all β-lactams, fluoroquinolones and aminoglycosides. Totally 27 β-lactamase genes and 2 membrane pore protein (porin) genes in 5 K. pneumoniae strains were screened by polymerase chain reaction (PCR). The results indicated that all of 5 K. pneumoniae strains carried blaTEM-1 and blaDHA-1 genes, as well as base deletion and mutation of OmpK35 or OmpK36 genes. Compared with carbapenem-sensitive isolates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), the resistant isolates...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Klebsiella pneumoniae; AmpC β-Lactamase; Porin; Molecular biology.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200015
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Caryocar brasiliense camb protects against genomic and oxidative damage in urethane-induced lung carcinogenesis BJMBR
Colombo,N.B.R.; Rangel,M.P.; Martins,V.; Hage,M.; Gelain,D.P.; Barbeiro,D.F.; Grisolia,C.K.; Parra,E.R.; Capelozzi,V.L..
The antioxidant effects of Caryocar brasiliense Camb, commonly known as the pequi fruit, have not been evaluated to determine their protective effects against oxidative damage in lung carcinogenesis. In the present study, we evaluated the role of pequi fruit against urethane-induced DNA damage and oxidative stress in forty 8-12 week old male BALB/C mice. An in vivo comet assay was performed to assess DNA damage in lung tissues and changes in lipid peroxidation and redox cycle antioxidants were monitored for oxidative stress. Prior supplementation with pequi oil or its extract (15 µL, 60 days) significantly reduced urethane-induced oxidative stress. A protective effect against DNA damage was associated with the modulation of lipid peroxidation and low...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pequi; Caryocar brasiliense Camb; Oxidative stress; Lung cancer; Urethane; Molecular biology.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900852
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Characterization of Tomato yellow spot virus, a novel tomato-infecting begomovirus in Brazil PAB
Calegario,Renata Faier; Ferreira,Sávio de Siqueira; Andrade,Eduardo Chumbinho de; Zerbini,Francisco Murilo.
The objective of this work was the biological and molecular characterization of a begomovirus detected in São Joaquim de Bicas, Minas Gerais, Brazil, named TGV-[Bi2], by determining its host range, complete nucleotide sequence and phylogenetic relationships with other begomoviruses. Biological characterization consisted of a host range study using either sap inoculation or particle bombardment as inoculation methods. The yellow spot virus can infect plants in Solanaceae and Amaranthaceae, including economically importat crops as sweet pepper, and weeds as Datura stramonium and Nicotiana silvestris. For the molecular characterization, the full-length genome (DNA-A and DNA-B) was amplified, cloned and completely sequenced. Sequence comparisons and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lycopersicon esculentum; Bemisia tabaci; ToYSV; Molecular biology; Geminivirus; Whitefly.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000900016
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Cytogenetic and molecular analyses of Oryza sativa L. x O. australiensis Domin. and O. sativa L. x O. brachyantha A. Chev. et Roehr derivatives International Rice Research Institute
Abbasi, Fida M..
xv, 119 leaves : ill. Thesis (Ph.D.) -- University of the Philippines at Los Baños
Tipo: Thesis Palavras-chave: Rice; Cytogenetics; Hybrids; Wild rice; Plant breeding; Molecular biology.
Ano: 1999 URL: http://hdl.handle.net/123456789/536
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Detection of mutations within exons 4 to 8 of the p53 tumor suppressor gene in canine mammary glands Arq. Bras. Med. Vet. Zootec.
Souza,D.M.B.; Barros,M.G.O.; Silva,J.S.C.; Silva,M.B.; Coleto,Z.F.; Jimenez,G.C.; Adrião,M.; Wischral,A..
Fifteen female canines with mammary tumors and 6 normal females were used to study mutations in exons 4 to 8 of the p53 gene. DNA samples from the tumors, respective adjacent normal mammary tissue and mammary glands from healthy animals were sequenced and analyzed for the presence of mutations. Mutations were found in 71.8% of the samples and the most frequent were missense mutations. The most attacked exons in the mammary tumor were 5, 7 and 8, with 23.4, 31.6 and 23.4% mutations, respectively. Canine mammary tumors are related to mutations in gene p53 and mutations mostly occur in the region of the protein that is linked to the DNA in the cell nucleus, which can change the functionality of the cell and propitiate tumor growth. Despite being...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bitch; DNA; Mamma; Molecular biology; Neoplasm; PCR.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000200013
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Detection of Mycobacterium avium subsp. paratuberculosis in bovine milk from the state of Pernambuco, Brazil BJM
Albuquerque,Pedro Paulo Feitosa de; Santos,André de Souza; Souza Neto,Orestes Luiz de; Kim,Pomy de Cássia Peixoto; Cavalcanti,Erika Fernanda Torres Samico Fernandes; Oliveira,Júnior Mário Baltazar de; Mota,Rinaldo Aparecido; Pinheiro Júnior,José Wilton.
Abstract The aim of this study was to detect the IS900 region of Mycobacterium avium subsp. paratuberculosis (MAP) in bovine milk samples using real-time polymerase chain reaction (qPCR) and conventional PCR, and to study the agreement between these tests. A total of 121 bovine milk samples were collected from herds considered positive for MAP, from the State of Pernambuco, Brazil. MAP DNA was detected in 20 samples (16.5%) using conventional PCR and in 34 samples (28.1%) using qPCR. MAP DNA was detected in all of the 6 animal farms studied. Moderate agreement was found between qPCR and conventional PCR results, where the sensitivity and specificity of conventional PCR in relation to qPCR were 50% and 96.6%, respectively. Thus, the IS900 region of MAP was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular biology; Diagnosis; Paratuberculosis; Milk.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100113
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Déterminismes génétiques et écophysiologiques du fonctionnement des légumineuses : Conséquences sur la dynamique d’élaboration du rendement et de la qualité National Institute of Agronomic Research
Munier-Jolain, Nathalie; Salon, Christophe.
L'unité de recherche Génétique et Amélioration des plantes (URGAP DIJ) a comme axe prioritaire de recherche la qualité de la graine de légumineuse. Ses études portent sur le pois protéagineux (valeur nutritionnelle de la graine en alimentation animale, nutrition azotée de la plante) et s'appuient sur des espèces modèles dont notamment Medicago truncatula. Elles font appel aux méthodologies de la biologie moléculaire, cellulaire, de la culture in vitro, de l'expérimentation en serre et au champ.
Tipo: Working Paper Palavras-chave: Corn; Molecular biology; Céréale; Biologie moléculaire; Génétique; Valeur nutritive.
Ano: 2004 URL: http://hdl.handle.net/2174/86
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Developing a rapid, efficient and low cost method for rapid DNA extraction from arthropods Ciência Rural
Mega,Nicolás Oliveira; Revers,Luís Fernando.
Here, it is presented a rapid and efficient method to obtain good quality DNA from small samples of arthropod tissues generating low quantities of hazardous wastes. This new method was compared with another homemade protocol using phenol and other two commercial kits. The quality of DNA obtained was checked by spectrophotometer and evaluated by an AFLP assay. Low shearing DNA was obtained from all samples and the best readings were observed to DNA recollected with the new method. The AFLP assay indicated that DNA obtained with all methods were suitable for use in molecular biology techniques sensitive to contaminants. However, homemade protocols were more efficient in recollect DNA than commercial kits, without lose any quality of samples. Also, they were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arthropod DNA; High quality DNA; Molecular biology; AFLP.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000900012
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Development of a Rapid and Sensitive Method for Detection of African Swine Fever Virus Using Loop-Mediated Isothermal Amplification BABT
Wu,Xulong; Xiao,Lu; Wang,Yin; Yang,Zexiao; Yao,Xueping; Peng,Bin.
ABSTRACT A loop-mediated isothermal amplification (LAMP) assay was developed for rapid, sensitive and specific detection of African swine fever virus (ASFV). A set of LAMP primers was designed based on the sequence of the ASFV gene K205R. Reaction temperature and time were optimized to 64 oC and 60 min, respectively. LAMP products were detected by agarose gel electrophoresis or visually with the addition of fluorescent dye. The detection limit of the LAMP assay was approximately 6 copies of the target gene per microliter, 100 times more sensitive than conventional PCR. LAMP is a simple and inexpensive molecular assay format for ASFV detection. To date, African swine fever has not been reported in China. LAMP can be used to monitor ASFV spread into China,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: African swine fever virus; LAMP; K205R gene; Molecular biology.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000200400
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Diagnostic methods for identification of root-knot nematodes species from Brazil Ciência Rural
Cunha,Tiago Garcia da; Visôtto,Liliane Evangelista; Lopes,Everaldo Antônio; Oliveira,Claúdio Marcelo Gonçalves; God,Pedro Ivo Vieira Good.
ABSTRACT: The accurate identification of root-knot nematode (RKN) species (Meloidogyne spp.) is essential for implementing management strategies. Methods based on the morphology of adults, isozymes phenotypes and DNA analysis can be used for the diagnosis of RKN. Traditionally, RKN species are identified by the analysis of the perineal patterns and esterase phenotypes. For both procedures, mature females are required. Over the last few decades, accurate and rapid molecular techniques have been validated for RKN diagnosis, including eggs, juveniles and adults as DNA sources. Here, we emphasized the methods used for diagnosis of RKN, including emerging molecular techniques, focusing on the major species reported in Brazil.
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA; Esterase; Meloidogyne; Molecular biology; Morphological pattern.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200153
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Diagnóstico molecular para o nematóide Rotylenchulus reniformis. Infoteca-e
CORDEIRO, M. C. R.; GOULART, A. M. C.; COSTA, A. M.; SHARMA, R. D..
bitstream/CPAC-2010/30255/1/bolpd-214.pdf
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Biologia molecular; Nematóide; Rotylenchulus reniformis; Molecular biology; Nematoda.
Ano: 2008 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/571879
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Efecto de la levadura Saccharomyces cereviciaie desintegrada y tres fracciones en una línea celular y como aditivo alimentario en el cultivo de Artemia OceanDocs
Comabella Soto, Y.; Centro de Investigaciones Marinas Universidad de La Habana.
En el presente estudio se evalúa el efecto que la levadura Saccharomyces cerevisiae de forma desintegrada (LD) y tres fracciones obtenidas de ella (complejo pared-membrana (FP), núcleo- proteínas (FN) y citoplasma (FC)) produce en dos modelos biológicos experimentales: la línea celular MB16-F10 y la especie Artemia franciscana. Para los estudios celulares se empleó la parte soluble de cada una de estas fracciones probándose las concentraciones de 0.5, 1, 2, 5 y 10 µg/mL adicionándose el valor de 25 µg/mL para LD y FC. Se determinó el efecto en la proliferación, vitalidad y fases del ciclo celular a las 48 y 72 horas. Como resultado se obtuvo una acción estimulante de la proliferación celular con LD 5 µg/mL a las 48 horas y con FN 0.5 y 1 µg/mL a...
Tipo: Theses and Dissertations Palavras-chave: Molecular biology; Cell biology.
Ano: 2003 URL: http://hdl.handle.net/1834/5666
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Etude des facteurs de transcription impliqués dans l’accumulation lipidique en condition de stress azoté chez la microalgue haptophyte Isochrysis affinis galbana ArchiMer
Thiriet-rupert, Stanislas.
In every organism, evolution and acclimation to environmental changes are orchestrated by numerous molecular players. Among them, transcription factors (TFs) play a crucial role by regulating gene expression. Therefore, identify TFs involved in the production of high value products is a significant step in a biotechnological context. The laboratory has at its disposal a mutant strain of the haptophyte microalga Tisochrysis lutea producing twice more storage lipids than the wild type strain when exposed to nitrogen deprivation. Given the key role of TFs in phenotype establishment, this PhD aim at identify the TFs involved in that of the mutant phenotype of T. lutea. A TFs identification and classification pipeline was elaborated and applied to T. lutea’s...
Tipo: Text Palavras-chave: Bioinformatique; Biologie moléculaire; Évolution; Facteur de transcription; Micro-algue; Réseau de gènes; RNA-seq; Bioinformatic; Evolution; Gene network; Molecular biology; Microalgae; RNAseq; Transcription factor.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00371/48233/48368.pdf
Registros recuperados: 38
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