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Registros recuperados: 6
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Analysis of drug resistance mutations in pulmonary Mycobacterium tuberculosis isolates in the Southern coastal region of Andhra Pradesh, India BJID
Prasad,Polu Giri; Jasmine,Mohammad Shaik; kanta,Kota Neela Mani; Deepthi,Karumanchi; Allam,Uday Sankar.
ABSTRACT Purpose and objectives Detection of drug resistance plays a crucial role in tuberculosis (TB) treatment and prevention of Mycobacterium tuberculosis (MTB) transmission. The aim of this study was to determine the levels and patterns of resistance of MTB isolates to two key anti-TB drugs (rifampicin, RIF and isoniazid, INH) and the type of mutations in drug resistance genes (rpoB, katG and inhA) of the isolates at the southern coastal region of Andhra Pradesh, India, using commercially available GenoType MTBDRplus assay under the Revised National TB Control Program. Methods GenoType MTBDRplus assay was performed on 2859 sputum smear-positive samples and the mutations in the genes responsible for resistance (rpoB, katG and inhA) were analyzed....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Multidrug resistance; Mycobacterium tuberculosis; MTBDRplus assay; Mutations; Molecular detection.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000500281
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Genotyping of South American clinical isolates of Pythium insidiosum based on single nucleotide polymorphism-based multiplex PCR Ciência Rural
Weiblen,Carla; Azevedo,Maria Isabel de; Ianiski,Lara Baccarin; Stibbe,Paula Cristina; Pereira,Daniela Isabel Brayer; Zanette,Régis Adriel; Sangioni,Luís Antônio; Rivero,Rodolfo; Santurio,Janio Morais; Botton,Sônia de Avila.
ABSTRACT: We aimed to genotype the South American clinical isolates of Pythium insidiosum using the single nucleotide polymorphisms (SNP) of the ribosomal DNA sequences (rDNA). Previously, an SNP-based multiplex-PCR was able to distinguish three different clades of P. insidiosum isolates. Thus, we used this assay to evaluate South American clinical isolates of P. insidiosum (n=32), standard strains from Costa Rica (n=4), Thailand (n=3), Japan (n=1), and India (n=1), a standard strain of Pythium aphanidermatum, and Brazilian environmental isolates of Pythium torulosum, Pythium rhizo-oryzae and Pythium pachycaule voucher (n=3). It was possible to allocate each American P. insidiosum isolate to clade I, the isolates of India, Japan, and Thailand to clade II,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pythium insidiosum; Pythiosis; Molecular detection; Genotype; Single nucleotide polimorphisms.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000100450
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Molecular detection of Anaplasma platys in a naturally-infected cat in Brazil BJM
Lima,M.L.F; Soares,P.T; Ramos,C.A.N; Araújo,F.R; Ramos,R.A.N; Souza,I.I.F; Faustino,M.A.G; Alves,L.C.A.
Following the accidental finding of inclusion bodies similar to Anaplasma platys in a stained blood smear from a cat, DNA analysis of the 16S rRNA gene was performed and 100% identity was found with different strains of A. platys. These data confirm that cats are susceptible to parasitism by A. platys.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Feline; Anaplasma platys; Molecular detection.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200019
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Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds Rev. bras. sementes
Barrocas,Ellen Noly; Machado,José da Cruz; Almeida,Mirella Figueiró de; Botelho,Luana Silva; Pinho,Édila Vilela de Resende Von.
Maize seeds, infected by Stenocarpella species, are important sources of inoculum for the introduction and dissemination of stalk and ear rot and macrospore leaf spot diseases. The use of healthy seeds is an important strategy for the preventive control of these diseases. However, one of the difficulties in the health quality control programs for maize seeds is the availability of a reliable and quick method for detecting these fungi during routine seed analyses. Therefore, the objective of the present study was to investigate the possibility of using the PCR technique as an alternative method for accurately detecting these pathogens in maize seed samples. Maize seeds were kept in contact with S. maydis colonie developed in PDA media containing mannitol at...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungus; Molecular detection; Stalk and ear rot; Macrospore leaf spot.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222012000200005
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Use of FTA elute card impregnated with cervicovaginal sample directly into the amplification reaction increases the detection of human papillomavirus DNA BJM
Santos,Carla R.; Franciscatto,Laura G.; Barcellos,Regina B.; Almeida,Sabrina E. M; Rossetti,Maria Lucia R..
This study aimed to evaluate the use of the FTA elute cardTM impregnated with cervicovaginal sample directly in the PCR amplification for detection of HPV-DNA. The results were compared to a reference technique. This method was more efficient than the protocol indicated by the manufacturer, identifying 91.7% against 54.2% of the positive samples.
Tipo: Info:eu-repo/semantics/article Palavras-chave: HPV; Cervical cancer; FTA elute; Molecular detection.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100047
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Use of the polymerase chain reaction for detection of Fusarium graminearum in bulgur wheat Ciênc. Tecnol. Aliment.
Faria,Carla Bertechini; Almeida-Ferreira,Giovana Caputo; Gagliardi,Karina Bertechine; Alves,Tatiane Cristina Albuquerque; Tessmann,Dauri José; Machinski Junior,Miguel; Barbosa-Tessmann,Ione Parra.
The detection of mycotoxigenic fungi in foodstuff is important because their presence may indicate the possible associated mycotoxin contamination. Fusarium graminearum is a wheat pathogen and a producer of micotoxins. The polymerase chain reaction (PCR) has been employed for the specific identification of F. graminearum. However, this methodology has not been commonly used for detection of F. graminearum in food. Thus, the objective of the present study was to develop a molecular methodology to detect F. graminearum in commercial samples of bulgur wheat. Two methods were tested. In the first method, a sample of this cereal was contaminated with F. graminearum mycelia. The genomic DNA was extracted from this mixture and used in a F. graminearum specific...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bulgur wheat; Fusarium graminearum; PCR; Molecular detection; Food protection.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000100030
Registros recuperados: 6
Primeira ... 1 ... Última
 

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