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| A recessive mutant cell line, B7, which is partially responsive to both interferon (IFN)-<FONT FACE="Symbol"> a</font> and IFN-<FONT FACE="Symbol">g</font> is described. B7 was FACS sorted from a cellular pool, which was obtained from the parental cell line 2C4, after several rounds of mutagenesis. The partial responsiveness to IFN was observed both in terms of expression of cell surface markers (CD2, class I and II HLAs) and mRNA expression of IFN-stimulated genes (9-27; 6-16; 2'-5' OAS; GBP and HLA-DRa). A genetic cross with the U4 mutant (JAK1-, a member of the Janus family of nonreceptor tyrosine kinase) did not restore full IFN-responsiveness to B7, and JAK1 cDNA transfection into B7 restored the wild phenotype of the cell... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Interferons; Transcriptional activation; Molecular genetics; JAK1; FACS analysis. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100007 |
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| Silva,Paula Viana Correa da; Lui,Jeffrey Frederico; Band,Guilherme de Oliveira; Regitano,Luciana Correia de Almeida; Grossi,Selma de Fátima; Sollero,Bruna Pena; Nunes,Cleujosí da Silva. |
| The aim of this work was to study the genetic variability among the wild boars, crossbred animals and pigs using microsatellite markers. Five genetic groups were studied. The fragments of three microsatellites developed for Sus scrofa domestica - IGF1, ACTG2 and TNFB - were amplified through PCR technique to evaluate the expected intra populacion variability (He) and observed (Ho) heterozygosity, and endogamy coefficient (F IS ) within each population and inter population variability F IS , testing relationship among five genetic groups to establish the genetic distance among them. The high level of observed heterozygosity values varied between 0.537 and 0.7871. Generally, F IS was low, suggesting that the endogamy did not exist between the tested animals. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular genetics; Wild boars; Microsatellites; Fragments; Amplification. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200011 |
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| Bonjardim,C.A.. |
| Mutant cell lines B3 and B10, which are unresponsive to both interferon (IFN)-alpha and IFN-gamma, and line B9, which does not respond to IFN-gamma stimulation, are described. The mutants were submitted to fluorescence-activated cell sorting (FACS) from a cellular pool, which was obtained from the parental cell line 2C4 after several rounds of mutagenesis. The unresponsiveness to IFN stimulation was observed both in terms of expression of cell surface markers (CD2, class I and II HLAs) and mRNA expression of IFN-stimulated genes (2'-5' oligoadenylate synthetase (OAS), 9-27, and guanylate binding protein (GBP)). Genetic crossing of B3, B9 and B10 with U3 (STAT1-), gamma2a (JAK2-) and U4 (JAK1-) mutants, respectively, did not restore IFN responsiveness to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Interferons; JAK/STAT; Molecular genetics; FACS analysis; RNase protection; Transcriptional activation. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100004 |
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| MAGALHAES, J. V. de; GUIMARAES, C. T.; SCHAFFERT, R. E.. |
| A toxidez causada pelo alumínio (Al) danifica o sistema radicular, restringindo a absorção de água e de nutrientes, resultando em perdas de produção por culturas cultivadas em solos ácidos. A toxidez de Al restringe o aprofundamento do sistema radicular, potencializando ainda os efeitos deletérios do estresse causado pela deficiência hídrica na produção vegetal. Em sorgo, a tolerância ao Al é conferida em grande parte pelo loco AltSB, mapeado na porção terminal do cromossomo 3. O gene que condiciona o loco AltSB, denominado SbMATE, é um transportador de citrato induzido e ativado pelo Al, que detoxifica o Al pela exclusão do metal de sítios sensíveis nos ápices radiculares. Vários marcadores moleculares foram desenvolvidos para o loco AltSB para permitir o... |
| Tipo: Folhetos |
Palavras-chave: Tolerância ao alumínio; SbMATE; Seleção assistida; Sorghum bicolor; Marcador genético; Genética molecular; Genetic markers; Molecular genetics. |
| Ano: 2013 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/982968 |
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| Oliveira,Eder Jorge; Pádua,Juliano Gomes; Zucchi,Maria Imaculada; Vencovsky,Roland; Vieira,Maria Lúcia Carneiro. |
| Microsatellites, or simple sequence repeats (SSRs), have been the most widely applied class of molecular markers used in genetic studies, with applications in many fields of genetics including genetic conservation, population genetics, molecular breeding, and paternity testing. This range of applications is due to the fact that microsatellite markers are co-dominant and multi-allelic, are highly reproducible, have high-resolution and are based on the polymerase chain reaction (PCR). When first introduced, the development of microsatellite markers was expensive but now new and efficient methods of repetitive sequence isolation have been reported, which have led to reduced costs and microsatellite-technology has been increasingly applied to several species,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microsatellites; Molecular genetics; Genetic structure of populations. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200018 |
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| Registros recuperados: 10 | |
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