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| Registros recuperados: 41 | |
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| A recessive mutant cell line, B7, which is partially responsive to both interferon (IFN)-<FONT FACE="Symbol"> a</font> and IFN-<FONT FACE="Symbol">g</font> is described. B7 was FACS sorted from a cellular pool, which was obtained from the parental cell line 2C4, after several rounds of mutagenesis. The partial responsiveness to IFN was observed both in terms of expression of cell surface markers (CD2, class I and II HLAs) and mRNA expression of IFN-stimulated genes (9-27; 6-16; 2'-5' OAS; GBP and HLA-DRa). A genetic cross with the U4 mutant (JAK1-, a member of the Janus family of nonreceptor tyrosine kinase) did not restore full IFN-responsiveness to B7, and JAK1 cDNA transfection into B7 restored the wild phenotype of the cell... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Interferons; Transcriptional activation; Molecular genetics; JAK1; FACS analysis. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100007 |
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| DIAS, V. S.; SILVA, J. G.; LIMA, K. M.; PETITINGA, C. S. C. D.; HERNÁNDEZ-ORTIZ, V.; LAUMANN, R. A.; PARANHOS, B. A. J.; URAMOTO, K.; ZUCCHI, R. A.; JOACHIM-BRAVO, I. S.. |
| An integrative multidisciplinary approach was used to delimit boundaries among cryptic species within the Anastrepha fraterculus complex in Brazil. Sexual compatibility, courtship and sexual acoustic behaviour, female morphometric variability, variation for the mitochondrial gene COI, and the presence of Wolbachia were compared among A. fraterculus populations from the Southern (Vacaria, Pelotas, Bento Gonçalves, S~ao Joaquim) and Southeastern (Piracicaba) regions of Brazil. Our results suggest full mating compatibility among A. fraterculus populations from the Southern region and partial pre-zygotic reproductive isolation of these populations when compared with the population from the Southeastern region. A. fraterculus populations from both regions... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Comportamento sexual; Seleção sexual; Inseto; Controle biológico; Praga; Cryptic species; Molecular genetics; Morphometrics; Reproductive isolation; Sexual behaviour. |
| Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/1053364 |
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| SANTOS, C.A.F.; SIMON, P.W.. |
| Linkage grouping and ordering AFLP markers analyses were performed for two different F2 populations of carrot (Daucus carota L.). Included were 277 and 242 dominant AFLP markers and 10 and 8 co-dominant markers assigned to the nine linkage groups of Brasilia x HCM and B493 x QAL F2 populations, respectively. The overall marker datasets, includjng repulsion and coupling phases, were consistently assigned to the nine linkage groups at LOD scores ranging from 3.5 to 5.0 in both F2 populations. Linkage grouping performed with only markers linked in coupling phase showed the nine linkage groups at LOD scores ranging from 3.0 to 7.5, 3.0 to 5.5, 3.5 to 5.0 and 2.5 to 8.5 in the Brasilia-, HCM-, B493- and QAL- coupling F2 population, respectively. The average... |
| Tipo: Artigo em anais de congresso (ALICE) |
Palavras-chave: Cenoura; Genoma; Marcador genético; Genética molecular; Daucus carota; AFLP; Genome; Genetic markers; Molecular genetics. |
| Ano: 2002 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/128418 |
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| SILVA JÚNIOR, J. V. J.; ARENHART, S.; SANTOS, H. F. dos.; ALMEIDA-QUEIROZ, S. R.; SILVA, A. N. M. R.; TREVISOL, I. M.; BERTANI, G. R.; GIL, L. H. V. G.. |
| The Infectious Bursal Disease Virus (IBDV) causes immunosuppression in young chickens. Advances in molecular virology and vaccines for IBDV have been achieved by viral reverse genetics (VRG). VRG for IBDV has undergone changes over time, however all strategies used to generate particles of IBDV involves multiple rounds of amplification and need of in vitro ligation and restriction sites. The aim of this research was to build the world?s first VRG for IBDV by yeast-based homologous recombination; a more efficient, robust and simple process than cloning by in vitro ligation. The wild type IBDV (Wt-IBDV-Br) was isolated in Brazil and had its genome cloned in pJG-CMV-HDR vector by yeast-based homologous recombination. The clones were transfected into chicken... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: IBVD; Virologia molecular; Frango; Doença de Gumboro; Genética molecular; Chickens; Infectious bursal disease virus; Avibirnavirus; Vertebrate viruses; Molecular genetics. |
| Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1026478 |
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| RAGOGNETTI, B. do N. N; STAFUZZA, N. B.; SILVA, T. B. R. da; CHUD, T. C. S.; GRUPIONI, V. A. R.; CRUZ, V. A. R.; DANTAS, J. de O.; NONES, K.; LEDUR, M. C.; MUNARI, D. P.. |
| Abstract: Selection among broilers for performance traits is resulting in locomotion problems and bone disorders, once skeletal structure is not strong enough to support body weight in broilers with high growth rates. In this study, genetic parameters were estimated for body weight at 42 days of age (BW42), and tibia traits (length, width, and weight) in a population of broiler chickens. Quantitative trait loci (QTL) were identified for tibia traits to expand our knowledge of the genetic architecture of the broiler population. Genetic correlations ranged from 0.56 +/- 0.18 (between tibia length and BW42) to 0.89 +/- 0.06 (between tibia width and weight), suggesting that these traits are either controlled by pleiotropic genes or by genes that are in linkage... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Mapeamento genético; Genética animal; Frango de corte; Parâmetro genético; Animal genetics; Molecular genetics; Broiler chicken. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/1045310 |
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| ANDREOTE, A. P. D.; ROSÁRIO, M. F. do; LEDUR, M. C.; JORGE, E. C.; SONSTEGARD, T. S.; MATUKUMALLI, L.; COUTINHO, L. L.. |
| MicroRNAs (miRNAs, miRs) encompass a class of small non-coding RNAs that often negatively regulate gene expression. miRNAs play an essential role in skeletal muscle, determining the proper development and maintenance of this tissue. In comparison to other organs and tissues, the full set of muscle miRNAs and its expression patterns are still poorly understood. In this report, a chicken skeletal muscle miRNA library was constructed, and the expression of selected miRNAs was further characterized during muscle development in chicken lines with distinct muscling phenotypes. Clone library sequence analysis revealed 40 small RNAs with similarities to previously described chicken miRNAs, seven miRNAs that were never identified before in chicken, and some... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Genetica animal; Galinha; Chickens; Molecular genetics; Cell proliferation. |
| Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1012924 |
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| Registros recuperados: 41 | |
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