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5S rDNA characterization in twelve Sciaenidae fish species (Teleostei, Perciformes): depicting gene diversity and molecular markers Genet. Mol. Biol.
Alves-Costa,Fernanda A.; Martins,Cesar; Matos,Fernanda Del Campos de; Foresti,Fausto; Oliveira,Claudio; Wasko,Adriane P..
In order to extend the genetic data on the Sciaenidae fish family, the present study had the purpose to characterize PCR-generated 5S rDNA repeats of twelve species of this group through PAGE (Polyacrylamide Gel Electrophoresis) analysis. The results showed the occurrence of at least two different 5S rDNA size classes in all the species. Moreover, 5S rDNA repeats of one of the studied species - Isopisthus parvipinnis - were cloned and subjected to nucleotide sequencing and Southern blot membrane hybridization analyses, which permitted to confirm the existence of two major 5S rDNA classes. Phylogenetic analysis based on the nucleotide sequences of different 5S rDNA repeats of I. parvipinnis lead to their separation into two major clusters. These results may...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sciaenidae; Fish; Isopisthus parvipinnis; 5S rDNA; Molecular markers.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200025
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A molecular marker approach using intron flanking EST-PCR to map candidate genes in peach (Prunus persica) Electron. J. Biotechnol.
Diez-de-Medina,Sergio; Silva,Herman.
In Peach (Prunus persica) several physiological changes, such as woolliness, triggered by chilling injury are involved in major production losses due to cold storage of the fruits during shipping. Additionally, the low level of polymorphisms among peach varieties is an important limitation in the search for new molecular markers that could be associated with economically important traits. Therefore, a functional approach was employed to associate candidate genes with an informative marker in peach. The data was obtained from the results of an in silico analysis of four different cold peach treatments. Thirty two candidate genes were selected that were aligned against Arabidopsis thaliana genomic sequences to design intron-flanking EST-PCR markers. These...
Tipo: Journal article Palavras-chave: Bin mapping; Molecular markers; Prunus; Rosaceae.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500007
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A note on the distribution of genetic diversity of Anglo-Nubian goats in central-northern farms of Piauí, Brazil R. Bras. Zootec.
Silvestre,Ellida de Aguiar; Costa,Márcio da Silva; Silva,Pollyana Oliveira da; Bajay,Miklos Maximiliano; Pinheiro,José Baldin; Zucchi,Maria Imaculada; Campelo,José Elivalto Guimarães; Britto,Fábio Barros.
The objective of this study was to characterize the genetic variability of Anglo-Nubian goats using microsatellite markers. The study was conducted using herds from four municipalities of Central-Northern Piauí (Teresina, José de Freitas, Campo Maior, and Angical), where technical information is scarce. Seven markers suggested by FAO were used (ILSTS11, McM527, INRA23, ETH10, OarfCB304, OarfCB48, and MAF209). The samples were genotyped using a 7% polyacrylamide gel. The average number of alleles per locus was 4.0, with observed and expected heterozygosity of 0.38 and 0.55, respectively. Few deviations from the Hardy-Weinberg equilibrium were observed for each population. Only two loci deviated significantly in two localities. The coefficient of gene...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endogamy; Genetic structure; Microsatellite; Molecular markers.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982015000400155
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A standardized protocol for genomic DNA isolation from Terminalia arjuna for genetic diversity analysis Electron. J. Biotechnol.
Sarwat,Maryam; Singh Negi,Madan; Lakshmikumaran,Malathi; Kumar Tyagi,Akhilesh; Das,Sandip; Shankar Srivastava,Prem.
For studying genetic diversity in natural populations of Terminalia, a medicinal plant, our attempts to isolate high quality DNA using several previously reported protocols and even modifications were unsuccessful. We therefore combined CTAB based isolation, and column based purification step, to isolate DNA from Terminalia arjuna. The DNA isolated using this standardized protocol was high in quality and suitable for restriction digestion and generation of random amplification of polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP).
Tipo: Journal article Palavras-chave: DNA extraction; Medicinal plants; Molecular markers; Terminalia species.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100011
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Advances in research and development of mango industry Rev. Bras. Frutic.
Bally,Ian S. E..
World mango production is spread over 100 countries that produce over 34.3 million tons of fruit annually. Eighty percent of this production is based in the top nine producing nations that also consume upward of 90% of their production domestically. One to 2 percent of fruit is traded internationally in to markets in the European Community, USA, Arabian Peninsula and Asia. This paper outlines some of the recent research and development advances in mango breeding and genomics, rootstock development, disease management and harvest technologies that are influencing the production and quality of mango fruit traded domestically and internationally.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Australia; Breeding; Molecular markers; Rootstocks; Anthracnose; Harvesting; Sap-burn.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452011000500008
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AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions Agronomy
Andrade, Felipe Aranha de; Gonçalves, Leandro Simões Azeredo; Miglioranza, Édison; Ruas, Claudete de Fátima; Ruas, Paulo Mauricio; Takahashi, Lúcia Sadayo Assari.
The present study aimed to estimate and characterize the genetic divergence between determinate and indeterminate snap bean accessions from the Universidade Estadual de Londrina (UEL) germplasm bank based on amplified fragment length polymorphism (AFLP) markers. A total of 40 and 32 accessions with determinate and indeterminate growth habits, respectively, were characterized for this purpose. Seven combinations of primers corresponding to EcoR1 and Mse1 were tested for the AFLP analysis, and the combinations E-AAG/M-CTC, E-ACT/M-CTT and E-ACC/M-CTT were selected. The resulting products were denatured and subjected to capillary electrophoresis. The Jaccard distance was used to estimate the genetic distances between accessions, and the Unweighted Pair...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genética e Melhoramento Phaseolus vulgaris L.; Molecular markers; Amplified Fragment Length Polymorphism; Gene bank; Genetic diversity Recursos Genéticos Phaseolus vulgaris L.; Molecular markers; Amplified fragment length polymorphism; Gene bank.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/25577
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AFLP markers differentiate isolates of Colletotrichum gossypii from C. gossypii var. cephalosporioides Trop. Plant Pathol.
Silva-Mann,Renata; Vieira,Maria G. G. Carvalho; Machado,José C.; Bernardino Filho,José Roberto; Salgado,Kalinka C. C.; Stevens,Mikel R..
Fungal diseases in cotton (Gossypium hirsutum), such as anthracnose caused by Colletotrichum gossypii and ramulose caused by C. gossypii var. cephalosporioides, are responsible for large yield losses. These pathogens are seed borne and morphologically similar although they induce different symptoms, which can lead to misdiagnosis using the blotter testing method. The present study was carried out to assess the viability of using Amplified Fragment Length Polymorphism (AFLP) markers to differentiate these pathogens. Five isolates, for each pathogen, were classified according to pathogenicity on cotton plants, and mycelial growth morphology. Conidial suspensions were sprayed on 30-day-old cotton plants and the symptoms assessed ten and 40 days after...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular markers; Anthracnose; Ramulose; Cotton diseases; Seed borne pathogens.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000200011
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Agronomic and molecular characterization of wild germplasm Saccharum spontaneum for sugarcane and energycane breeding purposes Scientia Agricola
Silva,Jorge Alberto Gonçalves da; Costa,Paulo Mafra de Almeida; Marconi,Thiago Gibbin; Barreto,Everton Jean da Silva; Solís-Gracia,Nora; Park,Jon-Won; Glynn,Neil Charles.
ABSTRACT: Among the species related to sugarcane, Saccharum spontaneum (L.) is a wild species with the greatest potential as a source of genetic variation to cope with biomass production in harsh environments. Due to its high yield, early vigor, ratooning ability, low input requirements and tolerance to various biotic and abiotic stresses, sugarcane breeders have shown interest in its contribution, as a donor of genes, to the development of high biomass energy canes. The conservation, evaluation and utilization of the genetic variability of S. spontaneum available in germplasm collections are critical for breeding, but, given the aggressive rhizomatous growth habit and the ability to propagate via seed dispersal, S. spontaneum is classified as a noxious...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular markers; Yield; Bioenergy; Abiotic stress; Core collection.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000400329
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Agrupamento de acessos e cultivares de três espécies de Brachiaria por RAPD - DOI: 10.4025/actasciagron.v30i4.5298 Agronomy
Ambiel, Ana Claudia; UNOESTE; Guaberto, Luciana Machado; UNOESTE; Vanderlei, Talita Marques; UNOESTE; Machado Neto, Nelson Barbosa; UNOESTE.
O propósito deste trabalho foi determinar a similaridade genética entre acessos de germoplasma e cultivares comerciais de três espécies de Brachiaria (inter e intraespecífica), por meio de marcadores RAPD. Sementes foram utilizadas como fonte de DNA. 10 “primers” decâmeros foram selecionados de 120 “primers” avaliados, produzindo 107 bandas polimórficas, as quais foram utilizadas para a análise de variância molecular (Amova), o coeficiente de similaridade de Jaccard e índices de fixação gênica. 24,40% da variabilidade genética total estão contidas entre as espécies e 75,60% dentro destas, com índice de fixação gênica (FST) 0,24. No dendrograma, houve a formação de dois ramos, um formado por P. maximum e B. arrecta e o outro subdividido em três: todas...
Palavras-chave: 5.02.01.03-4 Genética e Melhoramento Florestal Brachiaria; Marcadores moleculares; Variabilidade genética; Dendrograma; Índice de fixação gênica. Brachiaria; Molecular markers; Genetic variability; Dendrogram; Genetic fixation index.
Ano: 2008 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5298
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Analise da diversidade genetica do arroz silvestre americano (Oryza spp) atraves de marcadores moleculares. Repositório Alice
BUSO, G.S.C.; BERTOZO, M.R.; RANGEL, P.H.; FERREIRA, M.E..
1996
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Arroz silvestre; Diversidade genetica; Marcadores moleculares %% Wild rice; Genetic diversity; Molecular markers.
Ano: 1996 URL: http://www.alice.cnptia.embrapa.br/handle/doc/171468
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Análise da variabilidade genética de arnica (Lychnophora ericoides Less. - Asteraceae) usando marcadores RAPDS. Repositório Alice
MELO, L. Q.; CIAMPI, A. Y; VIEIRA, R. F..
bitstream/item/178594/1/SP-19821-ID-32083.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Marcadores moleculares; Plantas medicinais; Molecular markers; Cerrado.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/631377
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Analysis of genetic diversity among Indian niger [Guizotia abyssinica (L. f.) Cass.] cultivars based on randomly amplified polymorphic DNA markers Electron. J. Biotechnol.
Nagella,Praveen; Hosakatte,Niranjana Murthy; Ravishankar,K.V.; Hahn,Eun-Joo; Paek,Kee-Yoeup.
Randomly amplified polymorphic DNA (RAPD) markers were used to estimate genetic diversity among 18 cultivars of niger from India. Total genomic DNA was extracted and subjected to RAPD analysis using 80 arbitrary 10-mer primers; 17 primers were selected, which yielded a total of 124 bands, 41.20% of them polymorphic. None of the primers produced unique banding pattern for each cultivar. RAPD data were used to calculate a Squared-Euclidean Distance matrix which revealed a minimum genetic distance between cultivars JNC-6 and N-48 and a maximum distance between IGP-76 and JN-30. Based on the distance matrix, a cluster analysis was done using a minimum variance algorithm. The dendrogram generated, based on Ward’s method, grouped 18 niger cultivars...
Tipo: Journal article Palavras-chave: Genetic diversity; Guizotia abyssinica; Molecular markers; RAPD.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100014
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Analysis of genetic diversity and population structure in Argentine and Bolivian Creole cattle using five loci related to milk production Genet. Mol. Biol.
Lirón,J.P.; Ripoli,M.V.; De Luca,J.C.; Peral-García,P.; Giovambattista,G..
Data from five protein-coding loci related to dairy production were used to study the genetic diversity and population structure of Argentine and Bolivian Creole cattle breeds. Genomic DNA was extracted from blood samples of six Creole cattle breeds: Argentine (n = 230), Patagonian (n = 25); "Saavedreño" (n = 140), "Chaqueño Boliviano" (n = 30), "Yacumeño" (n = 27), and "Chusco" (n = 11). kappa-casein, beta-lactoglobulin, growth hormone and prolactin were measured by PCR-RFLP, while alphaS1-casein was typed by PCR-ASO. The results are discussed, focusing on: historical origin, recent differentiation and selection events, Zebu gene introgression, and population structure. This work shows that: (i) For the studied genes, the observed gene frequency profiles...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Creole cattle; Polymorphism; Genetic diversity; Molecular markers.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400010
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Analysis of genetic variability by ISSR markers in Calibrachoa caesia Electron. J. Biotechnol.
Pérez de la Torre,Mariana; García,Martín; Heinz,Ruth; Escandón,Alejandro.
Background: Calibrachoa Cerv. (ex La Llave & Lexarza) is a genus of the Solanaceae family (La Llave and Lexarza, 1825). This genus has a high ornamental and economic value due to its intrinsic variability and multiplicity of flower colours. In Argentina there are eight native species, and one of them is Calibrachoa caesia. The genetic diversity among 35 accessions of C. caesia, from five departments in the province of Misiones, was analyzed using ISSR markers. Results: Thirteen ISSR primers yielded a reproducible banding pattern, with 701 amplified loci and 98% of polymorphism. The ISSR primers 5’CT, 5’CA, 5’GA, 5’GACA, 3’CAC, 3’TG and 3’TC generated 100% polymorphic patterns. The Rp values ranged from 23.20 to 10.29 for 5’GACA and 3’AG primers,...
Tipo: Journal article Palavras-chave: Anchored microsatellites; Molecular markers; Ornamental plants.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500008
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Analysis of genetic variability of commercial melon cultivars using SSR molecular markers. Repositório Alice
CARVALHO, N.; CANELA, F. M.; LEITE, P. H. S.; FERREIRA, M. A.; OLIVEIRA, V. R.; SANTOS, M. F.; SOUZA, N. O. S.; BUSO, G. S. C..
bitstream/item/166721/1/Genetics-and-Molecular-Research-v.-16-n.-3-2017-CARVALHO-et-al.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Commercial cultivars; SSR; Melão; Marcador molecular; Cucumis melo; Genetic variability; Molecular markers.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1079736
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Artificial neural networks compared with Bayesian generalized linear regression for leaf rust resistance prediction in Arabica coffee PAB
Silva,Gabi Nunes; Nascimento,Moysés; Sant’Anna,Isabela de Castro; Cruz,Cosme Damião; Caixeta,Eveline Teixeira; Carneiro,Pedro Crescêncio Souza; Rosado,Renato Domiciano Silva; Pestana,Kátia Nogueira; Almeida,Dênia Pires de; Oliveira,Marciane da Silva.
Abstract: The objective of this work was to evaluate the use of artificial neural networks in comparison with Bayesian generalized linear regression to predict leaf rust resistance in Arabica coffee (Coffea arabica). This study used 245 individuals of a F2 population derived from the self-fertilization of the F1 H511-1 hybrid, resulting from a crossing between the susceptible cultivar Catuaí Amarelo IAC 64 (UFV 2148-57) and the resistant parent Híbrido de Timor (UFV 443-03). The 245 individuals were genotyped with 137 markers. Artificial neural networks and Bayesian generalized linear regression analyses were performed. The artificial neural networks were able to identify four important markers belonging to linkage groups that have been recently mapped,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffea arabica; Hemileia vastatrix; Artificial intelligence; Molecular markers; Prediction.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000300186
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Assessment of genetic diversity among Pakistani wheat (Triticum aestivum L.) advanced breeding lines using RAPD and SDS-PAGE Electron. J. Biotechnol.
Ahmed,Muhammad Farooq; Iqbal,Muhammad; Masood,Muhammad Shahid; Rabbani,Malik Ashiq; Munir,Muhammad.
Genetic diversity was assessed among 32 advanced wheat breeding lines included in the National Uniform Wheat Yield Trials (2006-07) of Pakistan using molecular (DNA) and biochemical (SDS-PAGE) markers. Of the 72 RAPD primers used for initial screening, 15 were found polymorphic. A total of 140 bands (61.4% polymorphic) were generated by the 15 random decamer primers. Genetic similarity coefficients ranged from 0.81 to 0.94 for rainfed and from 0.70 to 0.93 for the normal seeding date group. Cluster analysis using the unweighted pair group method of arithmetic averages (UPGMA) clustered the 32 advanced wheat breeding lines into one major and three small groups. Maximum level of polymorphism (90%) was observed for the primer OPA-05. Lines N9 and N11 showed...
Tipo: Journal article Palavras-chave: Genetic diversity; Molecular markers; Polymorphism; RAPD; SDS-PAGE; Wheat.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300001
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Assessment of genetic diversity on a sample of cocoa accessions resistant to witches' broom disease based on RAPD and pedigree data Bragantia
Santos,Ronaldo Carvalho dos; Pires,José Luís; Lopes,Uilson Vanderley; Gramacho,Karina Peres G.; Flores,Acassi Batista; Bahia,Rita de Cássia S.; Ramos,Helaine C. Cristine; Corrêa,Ronan Xavier; Ahnert,Dario.
Genetic diversity in cocoa (Theobroma cacao L.) has been assessed based on morphological and molecular markers for germplasm management and breeding purposes. Pedigree data is available in cocoa but it has not been used for assessing genetic relatedness. The geneitic diversity of 30 clonal cocoa accessions resistant to witche´ broom disease, from the CEPEC series, were studied on the basis of RAPD data and pedigree information. Twenty of these accessions descend from the TSA-644 clone, originated from a cross between the Upper Amazon germplasm called Scavina-6, the main source of resistance to witches' broom disease, and IMC-67. The ten remaining clones come from different sources including Amazon and Trinitario germplasm. RAPD data was collected using 16...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coefficient of parentage; Molecular markers.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052005000300005
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Assessment of silver-stained AFLP markers for studying DNA polymorphism in proso millet (Panicum miliaceum L.) Rev. Bras. Bot.
Karam,Décio; Westra,Philip; Niessen,Scott J.; Ward,Sarah M.; Figueiredo,José Edson Fontes.
Proso millet (Panicum miliaceum L.) is a serious weed in North America. A high number of wild proso millet biotypes are known but the genetic basis of its phenotypic variation is poorly understood. In the present study, a non-radioactive silver staining method for PCR-Amplified Fragment Length Polymorphism (AFLP) was evaluated for studying genetic polymorphism in American proso millet biotypes. Twelve biotypes and eight primer combinations with two/three and three/three selective nucleotides were used. Pair of primers with two/three selective nucleotides produced the highest number of amplified DNA fragments, while pair of primers with three/three selective nucleotides were more effective for revealing more polymorphic DNA fragments. The two better primer...
Tipo: Info:eu-repo/semantics/article Palavras-chave: AFLP; Genetic diversity; Molecular markers; Proso-millet.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042006000400011
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Assessment of somaclonal variation in somatic embryo-derived plants of yacon [Smallanthus sonchifolius (Poepp. and Endl.) H. Robinson] using inter simple sequence repeat analysis and flow cytometry Electron. J. Biotechnol.
Viehmannova,Iva; Bortlova,Zuzana; Vitamvas,Jan; Cepkova,Petra Hlasna; Eliasova,Katerina; Svobodova,Eva; Travnickova,Martina.
Background Yacon (Smallanthus sonchifolius) is a root crop native to the Andean region. Low sexual reproductive capacity is a major constraint facing the genetic breeding of this crop. Biotechnological techniques offer alternative ways to widen genetic variability. We investigated somaclonal variation in regenerants of yacon derived from in vitro somatic embryogenesis using simple sequence repeat (ISSR) analysis and flow cytometry. Results Twenty tested ISSR primers provided a total of 7848 bands in 60 in vitro regenerants and control plant. The number of bands for each primer varied from 3 to 10, and an average of 6.95 bands was obtained per ISSR primer. Eight primers were polymorphic and generated 10 polymorphic bands with 7.19% mean polymorphism. ISSR...
Tipo: Journal article Palavras-chave: DNA polymorphism; Molecular markers; Ploidy level; Proembryogenic callus.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000200008
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