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Registros recuperados: 10
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Determination of taxonomic relationships among brazilian taxa of Stylosanthes sw., leguminosae, using rapd markers. Repositório Alice
VIEIRA, M.L.C.; FUNGARO, M.H.P.; JUBIER, M.-F.; LEJEUNE, B..
A potencialidade dos RAPDs para estudos taxonomicos foi investigada em seis taxons de Stylosanthes Sw. Baseando-se na presenca/ausencia das bandas de RAPD, similaridades geneticas foram calculadas e um dendrograma construido de acordo com o metodo UPGMA. Dois grupos foram claramente diferenciados: um, representado por uma especie anual ( S. humilis), e outro, pelos taxons pertencentes ao complexo S. guianensis. Entre os taxons deste ultimo grupo, os RAPDs foram capazes de discriminar S. gracilis, a qual estaria sob processo de divergencia evolutiva.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Taxonomia molecular; Molecular taxonomy.
Ano: 1997 URL: http://www.alice.cnptia.embrapa.br/handle/doc/88835
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ENZYMATIC AND ANTAGONIST ACTIVITY OF ENDOPHYTIC FUNGI FROM Sapindus saponaria L. (SAPINDACEAE) Acta biol.Colomb.
SANTOS,Caroline Menicoze dos; RIBEIRO,Amanda da Silva; GARCIA,Adriana; POLLI,Andressa Domingos; POLONIO,Julio Cesar; AZEVEDO,João Lúcio; PAMPHILE,João Alencar.
ABSTRACT Endophyte microorganisms have great biotechnological interest, with features applicable to different areas and are potentially useful in agriculture. The current study determines the biotechnological potential of endophytic fungi, isolated from leaves of Sapindus saponaria, to control phytopathogenic fungi and evaluate their enzyme production. Molecular taxonomy was performed by sequencing of the ITS1-5.8S-ITS2 ribosomal DNA region, identifying the genera Phomopsis, Sordariomycetes, Diaporthe, and Colletotrichum. In vitro antagonism against phytopathogens showed better results against Fusarium solani and provided inhibition indices between 41.8 % and 67.5 %. The endophytic strain SS81 (Diaporthe citri) presented the highest antagonism index...
Tipo: Journal article Palavras-chave: Biological control; Endophytes; Enzymes; Microbial antagonisms; Molecular taxonomy.
Ano: 2019 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2019000200322
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Identification and taxonomy of some entomopathogenic Paecilomyces spp. (Ascomycota) isolates using rDNA-ITS Sequences Genet. Mol. Biol.
Inglis,Peter W.; Tigano,Myrian S..
A phylogenetic analysis of the 5.8S rDNA and internal transcribed spacer (ITS1 and ITS2) sequences from some entomogenous Paecilomyces species supports the polyphyly of the genus and showed the existence of cryptic species. In the Eurotiales, anamorphs Paecilomyces variotii and Paecilomyces leycettanus were related to the teleomorphs Talaromyces and Thermoascus. In the Hypocreales, three major ITS subgroups were found, one of which included Paecilomyces viridis, Paecilomyces penicillatus, Paecilomyces carneus and isolates identified as Paecilomyces lilacinus and Paecilomyces marquandii. However, the majority of the P. lilacinus and P. marquandii isolates formed a distinct and distantly related subgroup, while the other major subgroup contained Paecilomyces...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Paecilomyces spp.; Phylogenetic analysis; RDNA; Molecular taxonomy.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100025
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Meiofaunal cryptic species challenge species delimitation: the case of the Monocelis lineata (Platyhelminthes: Proseriata) species complex Naturalis
Scarpa, F.; Cossu, P.; Lai, T.; Sanna, D.; Curini-Galletti, M.; Casu, M..
Given the pending biodiversity crisis, species delimitation is a critically important task in conservation biology, but its efficacy based on single lines of evidence has been questioned as it may not accurately reflect species limits and relationships. Hence, the use of multiple lines of evidence has been portrayed as a means to overcome identification issues arising from gene/ species tree discordance, morphological convergence or recent adaptive radiations. Here, the integrative taxonomic approach has been used to address the study of the Monocelis lineata species complex. The taxonomic resolution of the complex is challenging, as the species lacks sclerotised copulatory structures, which as a rule of thumb aid identification in Proseriata. Eighteen...
Tipo: Article / Letter to the editor Palavras-chave: Integrative taxonomic approach; Meiofauna conservation; Molecular dating; Molecular phylogeny; Molecular taxonomy; RDNA 18S-28S.
Ano: 2016 URL: http://www.repository.naturalis.nl/record/607690
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One fungus, which genes? Development and assessment of universal primers for potential secondary fungal DNA barcodes Naturalis
Stielow, J.B.; Lévesque, C.A.; Seifert, K.A.; Meyer, W.; Irinyi, L.; Smits, D.; Renfurm, R.; Verkley, G.J.M.; Groenewald, M.; Chaduli, D.; Lomascolo, A.; Welti, S.; Lesage-Meessen, L.; Favel, A.; Al-Hatmi, A.M.S.; Damm, U.; Yilmaz, N.; Houbraken, J.; Lombard, L.; Quaedvlieg, W.; Binder, M.; Vaas, L.A.I.; Vu, D.; Yurkov, A.; Begerow, D.; Roehl, O.; Guerreiro, M.; Fonseca, A.; Samerpitak, K.; Diepeningen, A.D. van; Dolatabadi, S.; Moreno, L.F.; Casaregola, S.; Mallet, S.; Jacques, N.; Roscini, L.; Egidi, E.; Bizet, C.; Garcia-Hermoso, D.; Martin, M.P.; Deng, S.; Groenewald, J.Z.; Boekhout, T.; Beer, Z.W. de; Barnes, I.; Duong, T.A.; Wingfield, M.J.; Hoog, G.S. de; Crous, P.W.; Lewis, C.T.; Hambleton, S.; Moussa, T.A.A.; Al-Zahrani, H.S.; Almaghrabi, O.A.; Louis-Seize, G.; Assabgui, R.; McCormick, W.; Omer, G.; Dukik, K.; Cardinali, G.; Eberhardt, U.; Vries, M. de; Robert, V..
The aim of this study was to assess potential candidate gene regions and corresponding universal primer pairs as secondary DNA barcodes for the fungal kingdom, additional to ITS rDNA as primary barcode. Amplification efficiencies of 14 (partially) universal primer pairs targeting eight genetic markers were tested across > 1 500 species (1 931 strains or specimens) and the outcomes of almost twenty thousand (19 577) polymerase chain reactions were evaluated. We tested several well-known primer pairs that amplify: i) sections of the nuclear ribosomal RNA gene large subunit (D1–D2 domains of 26/28S); ii) the complete internal transcribed spacer region (ITS1/2); iii) partial β-tubulin II (TUB2); iv) γ-actin (ACT); v) translation elongation factor 1-α (TEF1α);...
Tipo: Article / Letter to the editor Palavras-chave: DNA barcoding; ITS supplement; Molecular taxonomy; Phylogeny; Species identification; Universal primers.
Ano: 2015 URL: http://www.repository.naturalis.nl/record/588731
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Preliminary notes on dual relevance of ITS sequences and pigments in Hygrocybe taxonomy . Naturalis
Babos, M.; Halász, K.; Zagyva, T.; Zöld-Balogh, Á.; Szegö, D.; Bratek, Z..
The relationships based on ITS sequences of 48 Hygrocybe s.l. specimens were studied and compared with previously described taxonomic groups. Our specimens formed two well separated genetic groups. The first one includes the species characterized by vivid yellow and red colours, while species belonging to other clades were pallid or pale brown, and in most cases with pink or olive tones. This separation is supported by the presence of muscaflavin pigments among some species referred to Hygrocybe (Bresinsky & Kronawitter 1986). The subgenera distinguished by morphological features can be relatively well recognized on phylogenetic trees, however, the majority of sections were not supported. Variability in the ITS region of Hygrocybe species is unusually...
Tipo: Article / Letter to the editor Palavras-chave: Hygrocybe; ITS region; Molecular taxonomy; Muscaflavin pigments.
Ano: 2011 URL: http://www.repository.naturalis.nl/record/532184
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Relaciones Evolutivas de las Especies del Genéro Lucifuga (Ophidiiformes: Bythitidae) en Cuba OceanDocs
Centro de Investigaciones Marinas Universidad de La Habana; Hernández Martínez, D..
En el presente trabajo se realizó un estudio de las relaciones evolutivas de las especies representativas de peces del género Lucifuga en Cuba mediante el análisis de sus variaciones genéticas y moleculares. Se estudiaron 11 sistemas proteicos y la secuencia parcial del gen mitocondrial citocromo b (cytb). Los animales fueron colectados en diferentes localidades de la zona occidental de Cuba. La filogenia reconstruida a partir de los caracteres moleculares indica la existencia de dos linajes evolutivos fundamentales cuya divergencia está sustentada por 11 loci proteicos, 73 sinapomorfías mitocondriales y un uso diferencial de los codones que codifican para el aminoácido valina. Uno de los linajes está constituido por las especies L. dentatus, L....
Tipo: Theses and Dissertations Palavras-chave: Genetics; Evolution (organisms); Molecular taxonomy.
Ano: 2005 URL: http://hdl.handle.net/1834/5530
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Searching for cryptic species in Erpobdella octoculata (L.) (Hirudinea: Clitellata): discordance between the results of genetic analysis and cross-breeding experiments Naturalis
Koperski, P.; Milanowski, R.; Krzyk, A..
The main aim of this study was to reveal reproduction barriers and potentially cryptic radiation within the very common and morphologically variable leech species - Erpobdella octoculata (L., 1758). The differences in reproductive success of the morphological forms were compared experimentally. The data based on the results of field sampling and analysis of ITS and COI sequences is also presented. The results of the analysis of DNA sequences clearly show lack of reproduction barriers between the analysed morphological forms. Subtle differences in DNA sequences between individuals, found with the use of the arbitrary primers method, seem to be related mainly to geographical distance between sub-populations. On the basis of these results, E. octoculata...
Tipo: Article / Letter to the editor Palavras-chave: COI; Cryptic species; Discordance; Hirudinea; ITS; Molecular taxonomy; 42.76.
Ano: 2011 URL: http://www.repository.naturalis.nl/record/369712
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Two Vibrio splendidus related strains collaborate to kill Crassostrea gigas: taxonomy and host alterations ArchiMer
Gay, Melanie; Renault, Tristan; Pons, Anne-marie; Le Roux, Frédérique.
For several years, strains phenotypically related to Vibrio splendidus have been associated with mortality outbreaks of molluscs. A former study on Crassostrea gigas demonstrated the genetic diversity of V splendidus strains associated with diseased animals. Another study suggested that different strains may act in an additive/synergistic way leading to higher C. gigas mortality rates. Here, a strain pair (31+32) was characterised at taxonomic and virulence levels. Using a polyphasic approach, these strains were confirmed to be V splendidus-related, without a clear discrimination between V. kanaloae and V. pomeroyi since hybridisation rates with both these strains were above 70%. Following experimental infection of C. gigas by injection in the adductor...
Tipo: Text Palavras-chave: Histopathology; Molecular taxonomy; Agonism; Vibriosis.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-2912.pdf
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Zoantharians (Hexacorallia: Zoantharia) associated with cold-water corals in the Azores Region: new species and associations in the deep sea ArchiMer
Carreiro-silva, Marina; Ocana, Oscar; Stankovic, David; Sampaio, Iris; Porteiro, Filipe M; Fabri, Marie-claire; Stefanni, Sergio.
Zoantharians are a group of cnidarians that are often found in association with marine invertebrates, including corals, in shallow and deep-sea environments. However, little is known about deep-sea zoantharian taxonomy, specificity and nature of their associations with their coral hosts. In this study, analyses of molecular data (mtDNA COI, 16S and 12S rDNA) coupled with ecological and morphological characteristics were used to examine zoantharian specimens associated with cold-water corals at depths between 110 and 800 m from seamounts and island slopes in the Azores region. The zoantharians examined were found living in association with stylasterids, antipatharians and octocorals. From the collected specimens, four new species were identified: (1)...
Tipo: Text Palavras-chave: Antipatharians; Gorgonians; Molecular taxonomy; Parasitic; Phylogeny; Stylasterids; Zoantharia.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00377/48818/49216.pdf
Registros recuperados: 10
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