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Registros recuperados: 22
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Adult somatic progenitor cells and hematopoiesis in oysters ArchiMer
Jemaa, Mohamed; Morin, Nathalie; Cavelier, Patricia; Cau, Julien; Strub, Jean Marc; Delsert, Claude.
Long-lived animals show a non-observable age-related decline in immune defense, which is provided by blood cells that derive from self-renewing stem cells. The oldest living animals are bivalves. Yet, the origin of hemocytes, the cells involved in innate immunity, is unknown in bivalves and current knowledge about mollusk adult somatic stem cells is scarce. Here we identify a population of adult somatic precursor cells and show their differentiation into hemocytes. Oyster gill contains an as yet unreported irregularly folded structure (IFS) with stem-like cells bathing into the hemolymph. BrdU labeling revealed that the stem-like cells in the gill epithelium and in the nearby hemolymph replicate DNA. Proliferation of this cell population was further...
Tipo: Text Palavras-chave: Hematopoiesis; Adult somatic progenitor cells; Hemocytes; Mollusk; Marine invertebrates.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00197/30802/29172.pdf
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Antimicrobial histones and DNA traps in invertebrate immunity: evidences in Crassostrea gigas. ArchiMer
Poirier, Aurore C.; Schmitt, Paulina; Rosa, Rafael D.; Vanhove, Audrey S.; Kieffer-jaquinod, Sylvie; Rubio, Tristan P.; Charriere, Guillaume M.; Destoumieux-garzon, Delphine.
Although antimicrobial histones have been isolated from multiple metazoan species, their role in host defense has long remained unanswered. We found here that the hemocytes of the oyster Crassostrea gigas release antimicrobial H1-like and H5-like histones in response to tissue damage and infection. These antimicrobial histones were shown to be associated with extracellular DNA networks released by hemocytes, the circulating immune cells of invertebrates, in response to immune challenge. The hemocyte-released DNA was found to surround and entangle vibrios. This defense mechanism is reminiscent of the neutrophil extracellular traps (ETs) recently described in vertebrates. Importantly, oyster ETs were evidenced in vivo in hemocyte-infiltrated interstitial...
Tipo: Text Palavras-chave: Antimicrobial Peptide (AMP); DNA; Innate Immunity; Invertebrate; Reactive Oxygen Species (ROS); NET; Mollusk.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00217/32866/31784.pdf
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Changes in the behavioral and immunological parameters of the mollusk Biomphalaria tenagophila induced by disruption of the circadian cycle as a consequence of continuous illumination BJMBR
Waissel,I.; Mineo,J.R.; Natal,C.L..
In the present investigation we studied some behavioral and immunological parameters of adult gastropod mollusk, Biomphalaria tenagophila, which have been reproducing for several generations under laboratory conditions. One group of gastropods was kept on a 14-h light/10-h dark cycle, corresponding to a regular circadian cycle, and another group was exposed to continuous light for 48 h. Animals were studied along (behavioral groups) or immediately after (immunological groups) 48 h of regular circadian cycle or continuous light conditions. Stopping/floating, dragging and sliding were the behavioral aspects considered (N = 20 for regular cycle; N = 20 for continuous illumination) and number of hemocytes/µl hemolymph was the immunological parameter studied (N...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Circadian cycle; Mollusk; Neuroimmunology; Stress; Behavior.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200014
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Characterization of GnRH-related peptides from the Pacific oyster Crassostrea gigas ArchiMer
Bigot, Laetitia; Zatylny-gaudin, Celine; Rodet, Franck; Bernay, Benoit; Boudry, Pierre; Favrel, Pascal.
Gonadotropin-releasing hormone (GnRH), a key neuropeptide regulating reproduction in vertebrates has now been characterized in a number of non-vertebrate species. Despite the demonstration of its ancestral origin, the structure and the function of this family of peptides remain poorly known in species as distant as lophotrochozoans. In this study, two GnRH-related peptides (Cg-GnRH-a and CgGnRH-G) were characterized by mass spectrometry from extracts of the visceral ganglia of the Pacific oyster Crassostrea gigas. These peptides showed a high degree of sequence identity with GnRHs of other mollusks and annelids and to a lesser extent with those of vertebrates or with AKH and corazonins of insects. Both the mature peptides and the transcript encoding the...
Tipo: Text Palavras-chave: Mollusk; Bivalve; Oyster; Neuroendocrinology; Reproduction; GnRH.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00079/19003/17477.pdf
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Development of quality index method for eviscerated and non-eviscerated octopus (Octopus insularis) Rev. Ciênc. Agron.
Aragão,Márcia Facundo; Garruti,Deborah dos Santos; Ogawa,Norma Barreto Perdigão; Bezerra,Viviane Costa; Silva,Elisabeth Mary Cunha da.
ABSTRACT This work aimed to develop a Quality Index Method (QIM) for octopus species Octopus insularis and apply it to samples of eviscerated and non-eviscerated specimens in order to establish stability in cold storage. Initially we carried out the recruitment of judges, who were trained to verify the changes undergone by the octopus during storage under refrigeration. The development of QIM Table took place in three experiments. At first, a QIM Table for Octopus vulgaris from literature was presented to judges together with specimens of O. insularis, so they could assess whether the parameters were suited to this specie. In the second experiment the objective was to validate the table, verifying if changes were needed in the predefined parameters or if...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mollusk; Sensorial analysis; Shelf life.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902019000200242
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Effect of stock density of cultured Amazon Apple Snail Pomacea dolioides (Gastropoda: Ampullariidae) in Brazil R. Bras. Zootec.
Pires-Júnior,Amilcar Nogueira; Hattori,Gustavo Yomar; Sant'Anna,Bruno Sampaio.
ABSTRACT This study evaluated the biomass gain, specific growth rate, absolute weight gain, and survival rate of the gastropod Pomacea dolioides cultivated in different stocking densities. To perform the experiment, samples were collected in March and June 2015. A total of 40 adult specimens were collected in the Jauary neighborhood (03°08′19.9"S, 58°27′32.5"W), municipality of Itacoatiara, AM, Brazil. They were kept in the laboratory for breeding to obtain the young P. dolioides, which were distributed into tanks containing 4 L of water and an aeration system, in three densities: 2.5 animals/L, 5 animals/L, and 10 animals/L, and were fed fish ration (34% gross protein). The specimens were measured for shell length and weight every fifteen days. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aquaculture; Growth; Mollusk; Stock enhancement.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100153
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Effects of a parental exposure to diuron on Pacific oyster spat methylome ArchiMer
Rondon Sallan, Rodolfo; Grunau, Christoph; Fallet, Manon; Charlemagne, Nicolas; Sussarellu, Rossana; Chaparro, Cristian; Montagnani, Caroline; Mitta, Guillaume; Bachere, Evelyne; Akcha, Farida; Cosseau, Celine.
Environmental epigenetic is an emerging field that studies the cause-effect relationship between environmental factors and heritable trait via an alteration in epigenetic marks. This field has received much attentions since the impact of environmental factors on different epigenetic marks have been shown to be associated with a broad range of phenotypic disorders in natural ecosystems. Chemical pollutants have been shown to affect immediate epigenetic information carriers of several aquatic species but the heritability of the chromatin marks and the consequences for long term adaptation remain open questions. In this work, we investigated the impact of the diuron herbicide on the DNA methylation pattern of spat from exposed Crassotrea gigas genitors. This...
Tipo: Text Palavras-chave: Crassostrea gigas; Mollusk; DNA methylation; Herbicide; Epigenetic.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00602/71413/69895.pdf
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Evidence of a bactericidal permeability increasing protein in an invertebrate, the Crassostrea gigas Cg-BPI ArchiMer
Gonzalez, Marcelo; Gueguen, Yannick; Destoumieux Garzon, Delphine; Romestand, Bernard; Fievet, Julie; Pugniere, M; Roquet, F; Escoubas, Jean-michel; Vandenbulcke, F; Levy, O; Saune, Laure; Bulet, P; Bachere, Evelyne.
A cDNA sequence with homologies to members of the LPS-binding protein and bactericidal/permeability-increasing protein (BPI) family was identified in the oyster Crassostrea gigas. The recombinant protein was found to bind LIPS, to display bactericidal activity against Escherichia coli, and to increase the permeability of the bacterial cytoplasmic membrane. This indicated that it is a BPI rather than an LPS-binding protein. By in situ hybridization, the expression of the C gigas BPI (Cg-bpi) was found to be induced in hemocytes after oyster bacterial challenge and to be constitutive in various epithelia of unchallenged oysters. Thus, Cg-bpi transcripts were detected in the epithelial cells of tissues/organs in contact with the external environment (mantle,...
Tipo: Text Palavras-chave: Oyster innate immunity; Mollusk; Hemocyte; Epithelia; Antimicrobial.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3564.pdf
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Giant african snail, Achatina fulica (Férussac, 1821): an environmental and public health problem in the northwestern of Paraná State, Brazil Biological Sciences
Andreazzi, Márcia Aparecida; Gasparotto, Francielli; Paccola, Edneia Aparecida de Souza; Silva, Cleiltan Novais da; Rodrigues, Aparecida de Fatima Cracco; Lizama, Maria de los Angeles Perez.
 Achatina fulica, known as Giant African Snail (GAS), was introduced in Brazil as a substitute for the European escargot, Helix aspersa maxima. However, its cultivation was abandoned and the mollusk became an invasive species, found throughout Brazil and causing damage to health and the environment. In this context, this study analyzed the knowledge of the population about the damage caused by GAS, seeking to reveal a regional scenario on this issue. This exploratory and cross-sectional study was conducted by means of a questionnaire applied to 150 people, which obtained sociodemographic characteristics of respondents and specific responses about the knowledge and handling of GAS. The results showed that most of the respondents know the snail and are...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mollusk; Introduced species; Environment; Social study..
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/35569
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Guide de la mensuration des espèces en halieutique, poissons, mollusques, crustacés, reptiles marins, mammifères marins ArchiMer
Badts, Vincent; Bertrand, Jacques.
This guide presents the rules for measuring fi shes, mollusks, shellfi shes, reptiles and marine mammals applicable to the fisheries surveys coordinated by Ifremer, including tasks carried out at the international level, in particular within the European regulation related to the fishery data collection.
Tipo: Text Palavras-chave: Mensuration; Taille; Poisson; Mollusque; Crustacé; Reptile marin; Mammifère marin; Halieutique; Protocole; Measurement; Size; Fishe; Mollusk; Shellfishe; Marine reptile; Marine mammal; Fishery; Guideline.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00001/6237/7655.pdf
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Inefficient immune response is associated with microbial permissiveness in juvenile oysters affected by mass mortalities on field ArchiMer
De Lorgeril, Julien; Escoubas, Jean Michel; Loubiere, Vincent; Pernet, Fabrice; Le Gall, Patrik; Vergnes, Agnes; Aujoulat, Fabien; Jeannot, Jean-luc; Jumas-bilak, Estelle; Got, Patrice; Gueguen, Yannick; Destoumieux-garzon, Delphine; Bachere, Evelyne.
Since 2008, juvenile Crassostrea gigas oysters have suffered from massive mortalities in European farming areas. This disease of complex etiology is still incompletely understood. Triggered by an elevated seawater temperature, it has been associated to infections by a herpes virus named OsHV-1 as well as pathogenic vibrios of the Splendidus clade. Ruling out the complexity of the disease, most of our current knowledge has been acquired in controlled experiments. Among the many unsolved questions, it is still ignored what role immunity plays in the capacity oysters have to survive an infectious episode. Here we show that juvenile oysters susceptible to the disease mount an inefficient immune response associated with microbial permissiveness and death. We...
Tipo: Text Palavras-chave: Host pathogen interaction; Innate immunity; Invertebrate; Mollusk; In situ mortality; Total bacteria; Crassostrea gigas.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00433/54470/55845.pdf
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Microscopic Anatomy of the Reproductive System in Two Sympatric Species of Fissurella Bruguiére, 1789 (Mollusca: Vetigastropoda) International Journal of Morphology
Collado,Gonzalo A; Brown,Donald I.
This study describes the microscopic anatomy of the reproductive systems of the keyhole limpets Fissurella latimarginata and Fissurella cumingi to test the existence of a possible isolating reproductive mechanism of morphophysiological type between species, and consequently to determine if the findings provide a valid taxonomic characteristic to distinguish these sympatric species. The results show that in both species the reproductive system is intimately associated with the excretory system. The gonad has a septa organization, and is connected with the distal portion of the right kidney by means of a renopericardial duct which functions as a genital duct. The gametes are evacuated to the mantle cavity through the right kidney opening. Besides transport...
Tipo: Journal article Palavras-chave: Mollusk; Fissurellidae; Reproductive system; Microscopic anatomy; Taxonomy.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000200012
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One Case of Triphallia in the Marine Snail Echinolittorina peruviana (Caenogastropoda: Littorinidae) International Journal of Morphology
Castillo,Viviana M; Brown,Donald I.
Gonochoric representatives of Littorinidae have a reproductive system organized with transit organs that connects the testicle with a penis specialized for internal fertilization. However, malformations at this level can produce, like triphallia (presence of triple penis). This description corresponds to one case of triphallia found in Echinolittorina peruviana (Lamarck, 1822) inhabitant of the South Pacific coasts. The macro and microscopic analysis reveals that every penis is an independent appendix whose individual morphology is similar to that in normal individuals, characterized by a non pigmented conic penis associated with a mammilliform gland located at the base of the right ocular tentacle. The cervical sperm groove lined by a simple ciliated and...
Tipo: Journal article Palavras-chave: Mollusk; Reproduction; Reproductive system; Penial morphology; Malformation; Littorinidae.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300003
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Oyster hemocytes express a proline-rich peptide displaying synergistic antimicrobial activity with a defensin ArchiMer
Gueguen, Yannick; Romestand, Bernard; Fievet, Julie; Schmitt, Paulina; Destoumieux Garzon, Delphine; Franck, Vandenbulcke; Philippe, Bulet; Bachere, Evelyne.
A cDNA sequence that encodes a 61-amino acid polypeptide precursor with homologies to proline-rich antimicrobial peptides (AMPs) was identified in the oyster Crassostrea gigas. After release of a hydrophobic signal peptide. the resulting 37-amino acid peptide, Cg-Prp, is composed of an acidic region and a cationic proline-rich region. To evaluate the biological properties of Cg-Prp, multiple proline-rich peptides corresponding to putative processing of the full-length Cg-Prp were synthesized. A limited antimicrobial activity was observed for two of them, which also showed strong synergistic antimicrobial activity with Cg-Def, a defensin from C gigas. To our knowledge, this is the first evidence of synergy between a defensin and another AMP in an...
Tipo: Text Palavras-chave: Antimicrobial peptide; Mollusk; Synergy; Invertebrate; Innate immunity; Pacific oyster.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6232.pdf
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Relaxing effect of eugenol and essential oils in Pomacea canaliculata Ciência Rural
Bianchini,Adriane Erbice; Cunha,Jessyka Arruda da; Brusque,Isabel Cristina Markowski; Pinheiro,Carlos Garrido; Schindler,Bianca; Heinzmann,Berta Maria; Baldisserotto,Bernardo.
ABSTRACT: This study evaluated the potential relaxing and/or molluscicidal effects of eugenol and essential oils of Origanum majorana, Ocimum americanum, Hesperozygis ringens, and Piper gaudichaudianum in the gastropod Pomacea canaliculata. Compounds were tested at concentrations of 100, 250, 500, and 750µL L-1 to evaluate the relaxing effects. In the second experiment, animals were exposed to 10, 25, and 50µL L-1 of essential oils of H. ringens and P. gaudichaudianum for a period of 24h for the evaluation of molluscicidal effects. Eugenol and essential oils of O. majorana and O. americanum showed relaxing effects at ≥250µL L-1, but the essential oils of H. ringens and P. gaudichaudianum did not promote relaxing or molluscicidal effects within the times...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anesthesia; Golden apple snail; Mollusk; Ocimum americanum; Origanum majorana.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001000403
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Spatial distribution of mollusks in the intertidal zone of sheltered beaches in southeastern of Brazil Zoologia
Arruda,Eliane P. de; Amaral,A. Cecilia Z..
The spatial distribution of mollusks in the intertidal zone was examined monthly from August 1995 through July 1997, in Enseada, Barra Velha and Araçá beaches in southeastern of Brazil. One study sector was selected in Enseada and Barra Velha, and two sectors in Araçá (Araçá I and Araçá II). The sectors were 10 m wide and equivalent in length to the width of the intertidal zone. Each sector was divided into three horizontal levels: lower, middle and upper, where the samples were taken with a cylinder corer with a base area of 0.16 m². In order to characterize the intertidal environment in these areas, some environmental variables were analyzed. In general, the mollusks were distributed in the sectors as follows: Enseada - Olivella minuta (Link, 1807) in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Caraguatatuba Bay; Intertidal zone; Mollusk; São Sebastião Channel; Sheltered beach; Spatial distribution.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752003000200020
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Temporal and spatial variation on heavy metal concentrations in the bivalve Perna perna (LINNAEUS, 1758) on the northern coast of Rio de Janeiro State, Brazil BABT
Ferreira,Alexandre Gomes; Machado,André Luís dos Santos; Zalmon,Ilana Rosental.
Heavy metal (Cd, Cr, Cu, Fe, Mn, Ni, Pb and Zn) concentrations were studied in Perna perna from three beaches (Barra do Furado, Buena and Ponta do Retiro) on the northern coast of Rio de Janeiro State by ICP-AES. The average concentration was 0.3 ± 0.04 (Cd); 1.9 ± 0.6 (Cr); 6.1 ± 0.7 (Cu); 1,130 ± 113 (Fe); 22 ± 3.2 (Mn); 9.3 ± 4.6 (Ni); 0.4 ± 0.2 (Pb); 44 ± 5.8 (Zn) µg.g-1 dry weight. There were no significant difference for almost all the studied metals in relation to sex. Although, significant spatial variations (p < 0.05) occurred for Cr, Pb and Ni with higher values for Barra do Furado. Temporal variations were significant (p < 0.05) at the three beaches, however, no trends of accumulation were observed. The metal concentrations were similar to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heavy metals; Mollusk; Spatial variation; Temporal variation.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200020
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The effect of stocking density on the growth of apple snails native Pomacea bridgesii and exotic Pomacea lineata (Mollusca, Gastropoda) Anais da ABC (AABC)
JUNIOR,ESMAR SOUZA; BARROS,JOSE CARLOS N. DE; PARESQUE,KARLA; FREITAS,RODRIGO R. DE.
The demand for alternative food sources is currently in evidence in the world and, therefore, the culture of animal species considered not conventional makes this theme relevant and appropriate. In the present study, the species Pomacea lineata and Pomacea bridgesii, each with three stowage densities (0.5 [T1], 1 [T2], and 1.5 [T3] animal/L), were tested. They were analyzed regarding growth rate, weight gain, final biomass, feed conversion and percentage of survival. There was not any statistically significant difference between the different densities for both species. The final average weight in the three waterworks did not differ significantly in P. bridgesii. In P. lineata, T1 (22.3 ± 1.80g) was significantly higher than T2 and T3. On the other hand,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mollusk; Aquaculture; Alternative food source; Feed conversion; Grow rate; Weight gain; Molusco; Aquicultura; Fonte alternativa de alimento; Conversao alimentar; Taxa de crescimento; Ganho de peso.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000200753
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The new insights into the oyster antimicrobial defense: Cellular, molecular and genetic view ArchiMer
Bachere, Evelyne; Rosa, Rafael Diego; Schmitt, Paulina; Poirier, Aurore; Merou, Nicolas; Charriere, Guillaume; Destoumieux-garzon, Delphine.
Oysters are sessile filter feeders that live in close association with abundant and diverse communities of microorganisms that form the oyster microbiota. In such an association, cellular and molecular mechanisms have evolved to maintain oyster homeostasis upon stressful conditions including infection and changing environments. We give here cellular and molecular insights into the Crassostrea gigas antimicrobial defense system with focus on antimicrobial peptides and proteins (AMPs). This review highlights the central role of the hemocytes in the modulation and control of oyster antimicrobial response. As vehicles for AMPs and other antimicrobial effectors, including reactive oxygen species (ROS), and together with epithelia, hemocytes provide the oyster...
Tipo: Text Palavras-chave: Mollusk; Immunity; Antimicrobial peptide; Hemocyte; Defensin.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00254/36552/35097.pdf
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Transcriptional changes in Crassostrea gigas oyster spat following a parental exposure to the herbicide diuron ArchiMer
Rondon, R.; Akcha, Farida; Alonso, P.; Menard, Dominique; Rouxel, Julien; Montagnani, Caroline; Mitta, Guillaume; Cosseau, C.; Grunau, C..
The Pacific oyster Crassostrea gigas is the main oyster species produced in the world, and a key coastal economic resource in France. High mortalities affect Pacific oysters since 2008 in France and Europe. Their origins have been attributed to a combination of biotic and abiotic factors, underlining the importance of environment quality. The impact of water pollution has been pointed out and one of the pollutants, the genotoxic herbicide diuron, occurs at high concentrations all along the French coasts. Previous work has revealed that a parental exposure to diuron had a strong impact on hatching rates and offspring development even if spats were not exposed to diuron themselves. In this study, we explored for the first time the transcriptional changes...
Tipo: Text Palavras-chave: Mollusk; Oyster; Diuron; Transcriptomic; Ecotoxicology.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00318/42938/42438.pdf
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