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Genetic stability in rice micropropagation Biocell
Medina,R; Faloci,M; Marassi,M.A.; Mroginski,L.A..
An efficient clonal propagation procedure for six rice varieties cultivated in Argentina was developed by using shoot tip cultures, and the genetic stability of the micropropagated plants was verified by isozyme analysis. One week old seedlings obtained on MS medium were sectioned and subcultured on MS medium (0.75% agar) supplemented with different combination and concentrations of cytokinins (BAP and KIN) and auxins (2,4-D and NAA). After four weeks of culture, multiple shoots were obtained. The best response was observed on MS supplemented with BAP 5 mg l-1. Shoot clumps were multiplied in MS liquid medium containing BAP 5 mg l-1. Profuse rooting was obtained after transfer to MS medium lacking growth regulators and with sucrose 8% (w/v). Complete...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Micropropagation; Multiple shoots; Oryza sativa L.; Isozyme; Genetic stability.
Ano: 2004 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000100002
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