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Fox grape cv. Bordô (Vitis labrusca L.) and grapevine cv. Chardonnay (Vitis vinifera L.) cultivated in vitro under different carbohydrates, amino acids and 6-Benzylaminopurine levels BABT
Carvalho,Dayse Cristina de; Silva,André Luís Lopes da; Schuck,Mariane Ruzza; Purcino,Marivel; Tanno,Guilherme Nakao; Biasi,Luiz Antonio.
The aim of this work was to study the influence of sucrose and glucose, amino acids and BAP (6-Benzylaminopurine) levels on in vitro shoot regeneration of fox grape cv. Bordô and grapevine cv. Chardonnay. The nodal segments from micropropagated material were used as explants and half-strength MS medium as the basal medium. Sucrose and glucose at 15, 30 and 45 g.L-1 were tested as a carbon source and the supplementation of adenine, asparagine, alanine, glycine, cysteine, glutamine, arginine was tested at 40 g.L-1. The BAP levels (1 and 5 μM) in solid and double-phase media were evaluated and compared with a control medium without BAP. Bordô had best in vitro growth than Chardonnay. Sucrose was a better carbohydrate source than glucose for both the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arginine; Nodal segment; Glucose; Adenine; Glycine.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200004
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Micropropagation of Glandularia perakii Cov. et Schn. (Verbenaceae): a native species with ornamental potential Biocell
Marino,Concepción; Ponce,María T.; Videla,María E.; Fioretti,Sonia; Cirrincione,Miguel.
Glandularia perakii is a perennial species with beautiful violet flowers that grows in the stony soil of Mendocine pedemont. A plentiful and prolonged flowering confers it an important ornamental potential. In this paper, a method of propagation of G. perakii from nodal segments is reported. Proliferating microshoot cultures were obtained by placing nodal segment on Murashige and Skoog medium (MS) supplemented with 20 g.L-1 of sucrose without growth regulators. In this medium multiplication rate after 20 days was 7.9. Rooted plants were acclimatized successfully.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Tissue culture; In vitro propagation; Nodal segment; Glandularia perakii.
Ano: 2003 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000100007
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