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| Registros recuperados: 32 | |
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| Barrozo, R.B.; Couton, L.; Lazzari, C.R.; Insauti, T.C.; Minoli, S.A.; Fresquet, N.; Rospars, J.P.; Anton, S.. |
| The haematophagous bug Rhodnius prolixus has been a model system in insect physiology for a long time. Recently, several studies have been devoted to its sensory systems, including olfaction. However, few data are available on the basic organisation of the nervous system in this species. By means of neuronal backfills, histology, confocal microscopy and three-dimensional reconstruction methods, we have characterized the projection patterns of antennal sensory neurons within the central nervous system of this disease-vector insect. We established the first partial three-dimensional map of the antennal lobe (AL) of a hemipteran insect. The ALs of this species are relatively diffuse structures, which nevertheless show a glomerular organisation. Based on... |
| Tipo: Journal Article |
Palavras-chave: RHODNIUS PROLIXUS; ARTHROPODA; HEXAPODA; INSECTE; HEMIPTERA; SYSTEME NERVEUX CENTRAL; NEURONE; OLFACTION; HISTOLOGIE; MICROSCOPIE CONFOCALE; CARTE TRIDIMENSIONNELLE; LOBE ANTENNAIRE; GLOMERULE; HETEROPTERA; REDUVIIDAE ANTENNAL LOBE; THREE-DIMENSIONAL RECONSTRUCTION; QUANTITATIVE ANALYSIS; DISEASE-VECTOR INSECT; ANTENNAL PROJECTIONS. |
| Ano: 2009 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010f9cf078d&uri=/notices/prodinra1/2010/11/ |
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| Durand, N.; Carot-Sans, G.; Chertemps, T.; Bozzolan, F.; Party, V.; Renou, M.; Debernard, S.; Rosell, G.; Maïbèche-Coisne, M.. |
| Background: Carboxyl/cholinesterases (CCEs) are highly diversified in insects. These enzymes have a broad range of proposed functions, in neuro/developmental processes, dietary detoxification, insecticide resistance or hormone/ pheromone degradation. As few functional data are available on purified or recombinant CCEs, the physiological role of most of these enzymes is unknown. Concerning their role in olfaction, only two CCEs able to metabolize sex pheromones have been functionally characterized in insects. These enzymes are only expressed in the male antennae, and secreted into the lumen of the pheromone-sensitive sensilla. CCEs able to hydrolyze other odorants than sex pheromones, such as plant volatiles, have not been identified. Methodology: In... |
| Tipo: Journal Article |
Palavras-chave: LEPIDOPTERA; NOCTUIDAE; HEXAPODA; PCR; ADNC; SDS PAGE; EXPRESSION DES GENES; ELECTROPHORESE; CARBOXYLESTERASE; SPODOPTERA LITTORALIS; OLFACTION; ODEEUR; PLANTE HOTE; INSECTE; ENZYME; BIOLOGIE MOLECULAIRE; BIOCHIMIE; ELECTROPHYSIOLOGIE; PHEROMONE; PHEROMONE SEXUELLE; RECOMBINAISON; PROTEINE. |
| Ano: 2010 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010229c7cd1&uri=/notices/prodinra1/2011/05/ |
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| Duportets, L.; Barrozo, R.B.; Bozzolan, F.; Gaertner, C.; Anton, S.; Gadenne, C.; Debernard, S.. |
| In the male moth Agrotis ipsilon behavioural response and antennal lobe (AL) neuron sensitivity to the female-produced sex pheromone increase with age and juvenile hormone (JH) level. We recently showed that the neuromodulator, octopamine (OA), interacts with JH in this age-dependent olfactory plasticity. To further elucidate its role, we cloned a full cDNA encoding a protein that presents biochemical features essential to OA/tyramine receptor (AipsOAR/TAR) function. The AipsOAR/TAR transcript was detected predominantly in the antennae, the brain and, more specifically, in ALs where its expression level varied concomitantly with age. This expression plasticity indicates that AipsOAR/TAR might be involved in central processing of the pheromone signal during... |
| Tipo: Journal Article |
Palavras-chave: LOBE ANTENNAIRE; LEPIDOPTERA; NOCTUIDAE; AGROPIS IPSILON; RECEPTEUR; OLFACTION; MITE; INSECTE; MATURATION SEXUELLE; ARTHROPODA; HEXAPODA; NEURONE; PHEROMONE; HORMONE; ADNC; CLONAGE; COMPORTEMENT SEXUEL; ATTRACTIF SEXUEL ANTENNAL LOBE; OCTOPAMINE/TYRAMINE RECEPTOR; OLFACTORY PROCESSING; MATURATION; EXPRESSION PLASTICITY. |
| Ano: 2010 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20102d18e75d&uri=/notices/prodinra1/2011/02/ |
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| Rospars, J.P.; Lansky, P.; Chaput, M.; Duchamp-Viret, P.. |
| Most olfactory receptor neurons (ORNs) express a single type of olfactory receptor that is differentially sensitive to a wide variety of odorant molecules. The diversity of possible odorant-receptor interactions raises challenging problems for the coding of complex mixtures of many odorants, which make up the vast majority of real world odors. Pure competition, the simplest kind of interaction, arises when two or more agonists can bind to the main receptor site, which triggers receptor activation, although only one can be bound at a time. Noncompetitive effects may result from various mechanisms, including agonist binding to another site, which modifies the receptor properties at the main binding site. Here, we investigated the electrophysiological... |
| Tipo: Journal Article |
Palavras-chave: CODAGE; MODELE; OLFACTION; NEURONE; RECEPTEUR; ODEUR; INTERACTION MOLECULAIRE; CONCENTRATION; ANALYSE STATISTIQUE SENSORY CODING; OLFACTORY RECEPTOR NEURON; IN VIVO; SIGNAL TRANSDUCTION; ALLOSTERY; MIXTURE INTERACTION. |
| Ano: 2008 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20105031bac0&uri=/notices/prodinra1/2010/10/ |
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| Coureaud, G.; Hamdani, Y.; Schaal, B.; Thomas-Danguin, T.. |
| The processing of odour mixtures by young organisms is poorly understood. Recently, the perception of an AB mixture, known to engage configural perception in adult humans, was suggested also to be partially configural in newborn rabbits. In particular, pups did not respond to AB after they had learned A or B. However, two alternative hypotheses might be suggested to explain this result: the presence in the mixture of a novel odorant that inhibits the response to the learned stimulus, and the unevenness of the sensory and cognitive processes engaged during the conditioning and the behavioural testing. We conducted four experiments to explore these alternative hypotheses. In experiment 1, the learning of A or B ended in responses to mixtures including a... |
| Tipo: Journal Article |
Palavras-chave: NEONATAL BEHAVIOUR; APPETITIVE LEARNING; CONFIGURAL PERCEPTION; OLFACTION; BLENDING MIXTURE; MAMMARY PHEROMONE; RABBIT. |
| Ano: 2009 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010fe95eab7&uri=/notices/prodinra1/2011/06/ |
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| Gabel, B.; Thiery, D.; Suchy, V.; Marion-Poll, F.; Hradsky, P.; Farkas, P.. |
| The European grapevine moth (EGVM), Lobesia botrana, is a major pest of grapes in Europe. Females are attracted to a nonhost plant: tansy (Tanacetum vulgare L.), which is a common weed in Slovakian vineyards. A steam distillate extract of tansy flowers was analyzed by means of a GC-EAG technique to screen constituents detected by the olfactory receptors of EGVM females. From more than 200 GC peaks, nine peaks corresponding to monoterpenoids released an EAG response in more than 70% of the females (N=15):p-cymene,d-limonene,α-thujene,α-thujone,β-thujone, thujyl alcohol, terpinene-4-ol, (Z)-verbenol, and piperitone. The steam distillate of tansy as well as a synthetic blend of identified compounds released consistent attraction in a field cage. The use of... |
| Tipo: Journal Article |
Palavras-chave: LOBESIA BOTRANA; TANACETUM VULGARE; TANAISIE; LEPIDOPTERA; TORTRICIDAE; ATTRACTION; DEPREDATEUR; CHROMATOGRAPHIE EN PHASE GAZEUSE; SPECTROMETRIE DE MASSE; ELECTROANTENNOGRAPHIE; MEDIATEUR CHIMIQUE; TERPENOIDE LOBESIA BOTRANA; VITIS VINIFERA; TANACETUM VULGARE; TANSY; GRAPEVINE; LEPIDOPTERA; TORTRICIDAE; COMPOSITAE; PLANT-INSECT RELATIONSHIPS; OLFACTION; ALLELOCHEMICALS; TERPENOIDS; COUPLING GC-EAG. |
| Ano: 1992 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB920002500600028663&uri=/notices/prodinra1/2010/11/ |
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| Laloi, D.; Roger, B.; Blight, M.M.; Wadhams, L.J.; Pham-Delègue, M.H.. |
| Individually restrained worker bees were trained to recognize complex odors in a conditioned proboscis extension assay. Three groups of bees were considered, based on the responses recorded during the experimental procedure: selective learners, nonselective learners, and nonlearners. For conditioning, three concentrations of two synthetic mixtures were used. The distribution of bees between groups was not significantly affected by the nature or by the concentration of the conditioning mixture. After conditioning, bees were tested with the individual compounds, and the responses were analyzed with respect to the three groups. Selective learners showed discriminative responses to a few key compounds, while nonselective learners responded to all the... |
| Tipo: Journal Article |
Palavras-chave: PROBOSCIS; ABEILLE DOMESTIQUE; APIS MELLIFERA; INSECTE UTILE; ODEUR; DIFFERENCIATION; APTITUDE HONEYBEE; LEARNING; PROBOSCIS EXTENSION; OLFACTION; ODOR MIXTURE. |
| Ano: 1999 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0400002758104388&uri=/notices/prodinra1/2010/11/ |
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| Le Bon, A.M.; Tromelin, A.; Thomas-Danguin, T.; Briand, L.. |
| La première étape de la détection olfactive implique l’activation de récepteurs olfactifs par les molécules odorantes. Ces récepteurs sont des protéines membranaires contenues dans la membrane des cils portés par les neurones sensoriels olfactifs localisés dans l’épithélium olfactif. Le codage de l’odeur résulte de l’activation combinatoire d’un ensemble de récepteurs et repose sur leur expression clonale. La connectique neuronale aboutit à la formation, dans le cortex, d’une image sensorielle olfactive qui est perçue consciemment en tant qu’odeur. Ce système combinatoire permet, avec seulement environ 340 récepteurs différents, de discriminer des myriades de molécules odorantes naturelles ou non (nouvelles flaveurs culinaires, molécules de synthèse,... |
| Tipo: Journal Article |
Palavras-chave: CODAGE OLFACTIF; ÉVOLUTION; ODEUR; OLFACTION; RECEPTEURS OLFACTIFS; MELANGES D’ODORANTS EVOLUTION; OLFACTION; ODOUR; ODOUR CODING; OLFACTORY RECEPTORS; ODORANT MIXTURES. |
| Ano: 2008 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20106fc5e9e8&uri=/notices/prodinra1/2011/04/ |
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| Barrozo, R.; Jarriault, D.; Simeone, X.; Gaertner, C.; Gadenne, C.; Anton, S.. |
| In the male moth, Agrotis ipsilon, mating induces a transient inhibition of behavioural and central nervous responses to sex pheromone. Newly mated males are not attracted to sex pheromone, and the sensitivity of their antennal lobe (AL) neurons is lower than in virgin males. This rapid transient olfactory inhibition prevents them from re-mating unsuccessfully until they have refilled their sex glands. We hypothesized that this olfactory ‘switch off’ might be controlled by neuromodulators such as biogenic amines. To test our hypothesis, we studied the effects of octopamine (OA) and serotonin (5-hydroxytryptamine, 5-HT) on the coding properties of pheromone-sensitive AL neurons in virgin and newly mated males. We show that AL neuron sensitivity increased in... |
| Tipo: Journal Article |
Palavras-chave: AGROTIS IPSILON; INSECTE; MITE; OLFACTION; ODEUR; LOBE ANTENNALE; PHEROMONE SEXUELLE; ATTRACTIF SEXUEL; SEROTONINE; PLASTICITE; SYSTEME NERVEUX CENTRAL; NEURONE; RECEPTEUR; OCTOPAMINE; NOCTUIDAE; LEPIDOPTERA OLFACTORY CODING; MOTH; ANTENNAL LOBE; SEX PHEROMONE; PROJECTION NEURON; MATING; OCTOPAMINE; SEROTONIN; PLASTICITY. |
| Ano: 2010 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010e7ad9fc3&uri=/notices/prodinra1/2010/09/ |
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| Anderson, A.R.; Wanner, K.W.; Trowell, S.C.; Warr, C.G.; Jacquin-Joly, E.; Zagatti, P.; Robertson, H.; Newcomb, R.D.. |
| Males and females of many moth species exhibit important differences in sexual behaviours. Much research in this field has focused on the male-specific behaviour, electrophysiology and molecular biology of sex pheromone reception. Female-specific behaviours have been less well studied although, like male-specific behaviours, they could provide opportunities for intervention and management of lepidopteran pests. Previously, we identified genes encoding putative odorant receptors (ORs) from the genome of the silkworm, Bombyx mori, some of which have higher levels of steady-state transcript in the antennae of adult females compared with males. We have identified the full-length cDNA sequences of some of these ORs and described a novel OR that is part of a... |
| Tipo: Journal Article |
Palavras-chave: TERPENE; ACIDE BENZOIQUE; PHENYLETHANOL; BENZALDEHYDE; ELECTROPHYSIOLOGIE; SEQUENCE NUCLEOTIDIQUE; ODEUR; HYPOTHENEMUS HAMPEI; ARTHROPODA; HEXAPODA; INSECTE; RECEPTEUR; PLANTE; COMPOSE VOLATILE; COMPORTEMENT SEXUEL; ATTRACTIF SEXUEL; ADNC; BIOLOGIE MOLECULAIRE; LEPIDOPTERA BOMBYX MORI; OLFACTION; ODORANT RECEPTORS; PLANT VOLATILES; PHEROMONE. |
| Ano: 2009 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20101ffb559b&uri=/notices/prodinra1/2010/11/ |
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| Chouquet, B.; Lucas, P.; Bozzolan, F.; Solvar, M.; Maïbèche-Coisné, M.; Durand, N.; Debernard, S.. |
| To clarify the role of phospholipase C (PLC) in insect olfactory transduction, we have undertaken its molecular identification in the moth Spodoptera littoralis. From the analysis of a male antennal expressed sequence tag library, we succeeded in cloning a full-length cDNA encoding a PLC that belongs to the cluster of PLC-b subtypes. In adult males, the PLC-b transcript was located predominantly in brain and antennae where its presence was detected in the olfactory sensilla trichodea. Moreover, PLC-b was expressed in antennae at the beginning of the pupal stage, then reached a maximum at the end of this stage and was maintained at this level during the adult period. Taken together, these results provided molecular evidence for the putative participation of... |
| Tipo: Journal Article |
Palavras-chave: INSECTE; PHOSPHOLIPASE; OLFACTION; ODEUR; MITE; TECHNIQUE ANALYTIQUE; SEQUENCE NUCLEOTIDIQUE; EXPRESSION DES GENES; ADNC; TRANSCRIPTION; NORTHERN BLOT; ELECTROPHORESE; PCR; CLONAGE MOLECULAIRE; ANALYSE PHYLOGENETIQUE; LEPIDOPTERA; NOCTUELLE MEDITERRANEENNE EXPRESSION PATTERNS; OLFACTORY SYSTEM; PHOSPHOLIPASE C; SPODOPTERA LITTORALIS; SIGNAL TRANSDUCTION. |
| Ano: 2010 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20108d7c7c75&uri=/notices/prodinra1/2010/09/ |
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| Le Danvic, C.; Guiraudie-Capraz, G.; Abderrahmani, D.; Zanetta, J.P.; Nagnan-Le Meillour, P.. |
| Knowledge of endogenous ligands of olfactory binding proteins is a prerequisite for studying their role in odor and pheromone transduction. Here, we report the extraction, derivatization, and characterization by gas chromatography-mass spectrometry of the natural ligands of pig, Sus scrofa (L.), Von Ebner’s Gland protein (VEG) and odorant binding protein (OBP). We identified two isoforms (VEG1 and VEG2), which differed only by the linkage of an O-N-acetylglucosamine (O-GlcNac) group on VEG1. The natural ligands of VEG1 were characterized as two isomers of testosterone, whereas ligands of VEG2 and OBP were fatty acids or their derivatives. Our findings suggest that the binding specificity of VEG1 for steroids is governed by the presence of an O-GlcNac... |
| Tipo: Journal Article |
Palavras-chave: MOLÉCULE ODORANTE ENDOGENOUS LIGAND; FATTY ACID; MALDI-TOF MASS SPECTROMETRY; ODORANT BINDING PROTEIN; OLFACTION; O-N-ACETYLGLUCOSAMINE; TESTOSTERONE; TRYTIC DIGESTION; VON EBNER'S GLAND PROTEIN. |
| Ano: 2009 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20099a3bdd2f&uri=/notices/prodinra1/2010/11/ |
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| Wright, G.A.; Mustard, J.A.; Simcock, N.K.; Ross-Taylor, A.A.R.; McNicholas, L.D.; Popescu, A.; Marion-Poll, F.. |
| Avoiding toxins in food is as important as obtaining nutrition. Conditioned food aversions have been studied in animals as diverse as nematodes and humans [1, 2], but the neural signaling mechanisms underlying this form of learning have been difficult to pinpoint. Honeybees quickly learn to associate floral cues with food [3], a trait that makes them an excellent model organism for studying the neural mechanisms of learning and memory. Here we show that honeybees not only detect toxins but can also learn to associate odors with both the taste of toxins and the postingestive consequences of consuming them. We found that two distinct monoaminergic pathways mediate learned food aversions in the honeybee. As for other insect species conditioned with salt or... |
| Tipo: Journal Article |
Palavras-chave: INSECTE; INSECTE UTILE; ALIMENTATION; TOXINE; APPRENTISSAGE; MEMOIRE; ODEUR; OLFACTION; DOPAMINE; SEROTONINE; SYSTME NERVEUX; ABEILLE DOMESTIQUE . |
| Ano: 2010 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201034c88ffd&uri=/notices/prodinra1/2011/05/ |
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| Registros recuperados: 32 | |
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