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Registros recuperados: 11
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Cadmium induced mitochondrial redox changes in germinating pea seed Inra
Smiri, M.; Chaoui, A.; Rouhier, N.; Chibani, K.; Gelhaye, É.; Jacquot, J.P.; El Ferjani, E.
Mitochondria play an essential role in producing the energy required for seedling growth following imbibition. Heavy metals, such as cadmium impair mitochondrial functioning in part by altering redox regulation. The activities of two protein redox systems present in mitochondria, thioredoxin (Trx) and glutaredoxin (Grx), were analysed in the cotyledons and embryo of pea (Pisum sativum L.) germinating seeds exposed to toxic Cd concentration. Compared to controls, Cd-treated germinating seeds showed a decrease in total soluble protein content, but an increase in –SH content. Under Cd stress conditions, Grx and glutathione reductase (GR) activities as well as glutathione (GSH) concentrations decreased both in cotyledons and the embryo. Similar results were...
Tipo: Journal Article Palavras-chave: PEA; COTYLEDONS; EMBRYO; HEAVY METALS; CADMIUM; GERMINATING SEEDS; MITOCHONDRIA; REDOX; THIOREDOXIN; GLUTAREDOXIN; GLUTATHIONE REDUCTASE; NADPH-DEPENDENT THIOREDOXIN REDUCTASE; NAD(P)H OXIDASE; OXIDATIVE STRESS.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201014da3708&uri=/notices/prodinra1/2010/11/
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Contribution of a PerR-like regulator to the oxidative-stress response and virulence of Enterococcus faecalis Inra
Verneuil, N.; Rincé, A.; Sanguinetti, M.; Posteraro, B.; Fadda, G.; Auffray, Y.; Hartke, A.; Giard, J.C..
PerR is one of the most important transcriptional regulators involved in the oxidative-stress response in Bacillus subtilis. Here, the homologous gene in Enterococcus faecalis, ranked among the leading causes of nosocomial infection, was characterized and analysed. Phenotype analysis showed that the perR mutant was significantly more resistant to H2O2 challenge (P<0·05). Expression of eight genes with potential roles in the oxidative-stress response was determined in the wild-type and perR-mutant strains by real-time quantitative PCR. Surprisingly, low quantitative differences in the transcriptional activity of these genes in the mutant versus wild-type were observed. Likewise, this locus was not involved in survival within murine macrophages, but in...
Tipo: Journal Article Palavras-chave: VIRULENCE; OXIDATIVE STRESS; GENE EXPRESSION REGULATION; TRANSCRIPTIONAL ACTIVITY; PERR GENE.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20076d61a643&uri=/notices/prodinra1/2009/11/
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Ferritins and iron storage in plants Inra
Briat, J.F.; Duc, C.; Ravet, K.; Gaymard, F..
Iron is essential for both plant productivity and nutritional quality. Improving plant iron content was attempted through genetic engineering of plants overexpressing ferritins. However, both the roles of these proteins in the plant physiology, and the mechanisms involved in the regulation of their expression are largely unknown. Although the structure of ferritins is highly conserved between plants and animals, their cellular localization differ. Furthermore, regulation of ferritin gene expression in response to iron excess occurs at the transcriptional level in plants, in contrast to animals which regulate ferritin expression at the translational level. In this review, our knowledge of the specific features of plant ferritins is presented, at the level...
Tipo: Journal Article Palavras-chave: IRON HOMEOSTASIS; OXIDATIVE STRESS; CHLOROPLAST; VACUOLE.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010daf77ff1&uri=/notices/prodinra1/2010/12/
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Ferritins control interaction between iron homeostasis and oxidative stress in Arabidopsis Inra
Ravet, K.; Touraine, B.; Boucherez, J.; Briat, J.F.; Gaymard, F.; Cellier, F..
Ferritin protein nanocages are the main iron store in mammals. They have been predicted to fulfil the same function in plants but direct evidence was lacking. To address this, a loss-of-function approach was developed in Arabidopsis. We present evidence that ferritins do not constitute the major iron pool either in seeds for seedling development or in leaves for proper functioning of the photosynthetic apparatus. Loss of ferritins in vegetative and reproductive organs resulted in sensitivity to excess iron, as shown by reduced growth and strong defects in flower development. Furthermore, the absence of ferritin led to a strong deregulation of expression of several metal transporters genes in the stalk, over-accumulation of iron in reproductive organs, and...
Tipo: Journal Article Palavras-chave: FERRITIN; IRON HOMEOSTASIS; OXIDATIVE STRESS; IRON STORAGE; IRON TRANSPORTER.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009dbcc10b7&uri=/notices/prodinra1/2010/12/
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Influence des traitements à base d'huiles essentielles sur les capacités de reproduction de Callosobruchus subinnotatus Pic. et de Callosobruchus maculatus F. (Coleoptera : Bruchidae) : mécanisme d'action de l'huile essentielle de Cymbopogon giganteus Chiov Inra
Nyamador, S..
Un inventaire de l’entomofaune lié aux stocks de voandzou a été réalisé à partir d’échantillons collectés dans six localités du Sud du Togo. L’identification des espèces a permis de noter la présence de Callosobruchus subinnotatus, le déprédateur habituel des stocks de voandzou et de Callosobruchus maculatus, qui est habituellement considéré comme le ravageur majeur des stocks de niébé. Selon les localités, les effectifs de C. subinnotatus représentent 0,24 à 4% seulement des effectifs d’insectes ravageurs sur le voandzou. Parmi les insectes identifiés, on note également la présence d’un parasitoïde solitaire nympholarvophage de bruches dont l’effectif est faible.Une évaluation de l’activité reproductrice de C. subinnotatus a été réalisée en faisant varier...
Tipo: Thesis Palavras-chave: PLANTE AROMATIQUE; HUILE ESSENTIELLE; TOXICITE; ENZYME; DETOXIFICATION; STRESS OXIDATIF; PRO OXYDANT; ACETYLCHOLINESTERASE; CATALASE; GLUTATHION REDUCTASE; GLUITATHION S-TRANSFERASE; SUPEROXYDE DISMUTASE; DEPREDATEUR; VOANDZOU; DENREE ALIMENTAIRE; LUTTE AROMATIC PLANT; ESSENTIAL OIL; TOXICITY; OXIDATIVE STRESS; INSECT PEST; FOOD; CONTROL.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20119f1fd6ee&uri=/notices/prodinra1/2011/01/
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Leptin downregulates heat shock protein-70 (HSP-70) gene expression in chicken liver and hypothalamus Inra
Figueiredo, D.; Gertler, A.; Cabello, G.; Decuypere, E.; Buyse, J.; Dridi, S..
Heat shock protein (HSP)-70 is expressed in normal and stressed cells but is highly stress-inducible. Although leptin has long been suggested to be involved in the regulation of stress response, its interaction with the HSP-70 gene is still unknown, under both unstressed and stressed conditions. The present study has aimed to investigate the effect of leptin on HSP-70 gene expression in normal chicken liver, hypothalamus, and muscle. Continuous infusion of recombinant chicken leptin (8 mu g/kg per hour) at a constant rate of 3 ml/h for 6 h in 3-week-old broiler chickens significantly (P < 0.05) decreased food intake and HSP-70 mRNA levels in liver and hypothalamus, but not in muscle. In an attempt to discriminate between the effect of leptin and of...
Tipo: Journal Article Palavras-chave: STRESS OXYDATIF; EXPRESSION GENIQUE; LEPTINE LEPTIN; HEAT SHOCK PROTEIN; FASTING; GENDER; OXIDATIVE STRESS; CHICKEN.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20077125ba1&uri=/notices/prodinra1/2008/01/
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New insights into ferritin synthesis and function highlight a link between iron homeostasis and oxidative stress in plants Inra
Briat, J.F.; Ravet, K.; Arnaud, N.; Duc, C.; Boucherez, J.; Touraine, B.; Cellier, F.; Gaymard, F..
Background: Iron is an essential element for both plant productivity and nutritional quality. Improving plant iron content was attempted through genetic engineering of plants overexpressing ferritins. However, both the roles of these proteins in plant physiology, and the mechanisms involved in the regulation of their expression are largely unknown. Although the structure of ferritins is highly conserved between plants and animals, their cellular localization differs. Furthermore, regulation of ferritin gene expression in response to iron excess occurs at the transcriptional level in plants, in contrast to animals which regulate ferritin expression at the translational level.Scope: In this review, an overview of our knowledge of bacterial and mammalian...
Tipo: Journal Article Palavras-chave: IRON; BACTERIOFERRITINS; FERRITINS; OXIDATIVE STRESS; IRON STORAGE; SEEDS; PATHOGENS; NUTRITION.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201022698fee&uri=/notices/prodinra1/2010/12/
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Pleiotropic role of quorum-sensing autoinducer 2 in Photorhabdus luminescens Inra
Krin, E.; Chakroun, N.; Turlin, E.; Givaudan, A.; Gaboriau, F.; Bonne, I.; Rousselle, J.C.; Frangeul, L.; Lacroix, C.; Hullo, M.F.; Marisa, L.; Danchin, A.; Derzelle, S..
Bacterial virulence is an integrative process that may involve quorum sensing. In this work, we compared by global expression profiling the wild-type entomopathogenic Photorhabdus luminescens subsp. laumondii TT01 to a luxS-deficient mutant unable to synthesize the type 2 quorum-sensing inducer AI-2. AI-2 was shown to regulate more than 300 targets involved in most compartments and metabolic pathways of the cell. AI-2 is located high in the hierarchy, as it controls the expression of several transcriptional regulators. The regulatory effect of AI-2 appeared to be dose dependent. The luxS-deficient strain exhibited decreased biofilm formation and increased type IV/V pilus-dependent twitching motility. AI-2 activated its own synthesis and transport. It also...
Tipo: Journal Article Palavras-chave: BACTERIAL VIRULENCE; OXIDATIVE STRESS; PROTEOME ANALYSIS; OXIDATIVE STRESS.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20085c5590af&uri=/notices/prodinra1/2009/03/
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Response to an ozone gradient of growth and enzymes implicated in tolerance to oxidative stress in Acer saccharum (Marsh.) seedlings Inra
Gaucher, C.; Costanzo, N.; Widden, P.; Renaud, J.P.; Dizengremel, P.; Mauffette, Y.; Chevrier, N..
Des semis d'érable à sucre (Acer saccharum Marsh.) de deux ans sont exposés en chambre à ciel ouvert à un large gradient d'O3 (0 to 300 nL.L-1) pendant 85 jours sous deux environnements lumineux (20 ou 80 % de plein soleil, journée ensoleillée à midi). Avec l'augmentation des concentrations d'O3, on observe une réduction de la croissance des semis ayant un ou deux flushs de feuilles. La réduction de croissance est particulièrement importante pour le deuxième flush de feuilles qui se développe pendant le traitement. Des dommages foliaires apparaissent durant la saison et sous fortes concentrations d'O3. La stratégie de croissance de survie de l'érable à sucre, montré par le rapport racine/tige, de même que les stimulations enzymatiques de la glucose...
Tipo: Journal Article Palavras-chave: STRESS OXYDATIF; RESISTANCE AU STRESS; OZONE; CROISSANCE CARBOXYLATION; DETOXIFICATION; GROWTH; OXIDATIVE STRESS.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20072af6c869&uri=/notices/prodinra1/2009/11/
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The chloroplastic thiol reducing systems: dual functions in the regulation of carbohydrate metabolism and regeneration of antioxidant enzymes, emphasis on the poplar redoxin equipment Inra
Chibani, K.; Couturier, J.; Selles, B.; Jacquot, J.P.; Rouhier, N..
The post-translational modification consisting in the formation/reduction of disulfide bonds has been the subject of intense research in plants since the discovery in the 1970s that many chloroplastic enzymes are regulated by light through dithiol–disulfide exchange reactions catalyzed by oxidoreductases called thioredoxins (Trxs). Further biochemical and proteomic studies have considerably increased the number of target enzymes and processes regulated by these mechanisms in many sub-cellular compartments. Recently, glutathionylation, a modification consisting in the reversible formation of a glutathione adduct on cysteine residues, was proposed as an alternative redox regulation mechanism. Glutaredoxins (Grxs), proteins related to Trxs, are efficient...
Tipo: Journal Article Palavras-chave: NADP-MALATE DEHYDROGENASE; METHIONINE SULFOXIDE REDUCTASE; FERREDOXIN-THIOREDOXIN REDUCTASE; GENOME-WIDE ANALYSIS; IRON-SULFUR PROTEIN; ARABIDOPSIS-THALIANA; CHLAMYDOMONAS-REINHARDTII; OXIDATIVE STRESS; GLUTATHIONE-PEROXIDASE; PHOTOOXIDATIVE STRESS; CHLOROPLAST; GLUTAREDOXIN; PHOTOSYNTHESIS; STRESS; THIOREDOXIN.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010751aa923&uri=/notices/prodinra1/2010/10/
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The fungal glutathione S-transferase system. Evidence of new classes in the wood-degrading basidiomycete Phanerochaete chrysosporium Inra
Morel, M.; Ngadin, A.A.; Droux, M.; Jacquot, J.P.; Gelhaye, É..
The recent release of several basidiomycete genome sequences allows an improvement of the classification of fungal glutathione S-transferases (GSTs). GSTs are well-known detoxification enzymes which can catalyze the conjugation of glutathione to non-polar compounds that contain an electrophilic carbon, nitrogen, or sulfur atom. Following this mechanism, they are able to metabolize drugs, pesticides, and many other xenobiotics and peroxides. A genomic and phylogenetic analysis of GST classes in various sequenced fungi—zygomycetes, ascomycetes, and basidiomycetes—revealed some particularities in GST distribution, in comparison with previous analyses with ascomycetes only. By focusing essentially on the wooddegrading basidiomycete Phanerochaete chrysosporium,...
Tipo: Journal Article Palavras-chave: BETA-ARYL ETHER; POLYCYCLIC AROMATIC-HYDROCARBONS; PRION PROTEIN URE2; SACCHAROMYCES-CEREVISIAE; OMEGA-CLASS; OXIDATIVE STRESS; SCHIZOSACCHAROMYCES-POMBE; PSEUDOMONAS-PAUCIMOBILIS; METABOLIZING ENZYMES; MOLECULAR-CLONING; PHANEROCHAETE CHRYSOSPORIUM; GLUTATHIONE S-TRANSFERASE; ETHERASE; OMEGA CLASS GST; URE2P; GTT.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20107588cb2a&uri=/notices/prodinra1/2010/10/
Registros recuperados: 11
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