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Registros recuperados: 6
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A comparison between enzyme immunoassay and HPLC for ochratoxin A detection in green, roasted and instant coffee BABT
Fujii,Simone; Ono,Elisabete Yurie Sataque; Ribeiro,Ricardo Marcelo Reche; Assunção,Fernanda Garcia Algarte; Takabayashi,Cássia Reika; Oliveira,Tereza Cristina Rocha Moreira de; Itano,Eiko Nakagawa; Ueno,Yoshio; Kawamura,Osamu; Hirooka,Elisa Yoko.
An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for ochratoxin A (OTA) detection in green, roasted and instant coffees was developed using anti-OTA monoclonal antibody. Immunological reagents prepared were OTA-BSA (4.76 mg/mL), anti-OTA.7 MAb (2x10³-fold dilution) and HRP-anti IgG (10³-fold dilution). The detection limit was 3.73 ng OTA/g and correlation coefficients (r) between this immunoassay and high performance liquid chromatography were 0.98 for green coffee, 0.98 for roasted and 0.86 for instant. OTA levels detected by ic-ELISA were higher than by HPLC, with ELISA/HPLC ratio of 0.66 - 1.46 (green coffee), 0.96 - 1.11 (roasted) and 0.93 - 1.82 (instant). ELISA recoveries for OTA added to coffee (5 - 70 ng/g) were 81.53 % for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ochratoxin; Immunoassay; HPLC; Monoclonal antibody; Coffee.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200020
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Evaluation of yeast cell wall on the performance of broiles fed diets with or without mycotoxins Rev. Bras. Ciênc. Avic.
Santin,E; Paulillo,AC; Nakagui,LSO; Alessi,AC; Maiorka,A.
This experiment aimed at evaluating the effects of the interactions between aflatoxin (500 or 250 ppb) and ochratoxin (500 or 250 ppb), and the possible benefits of adding yeast cell wall to prevent the effects of these mycotoxins in broiler chickens. Relative organ weight gain and live performance were evaluated at 21 and 42 days of age. Results indicated that at the levels of mycotoxins included in the experimental diets, ochratoxin reduced feed intake and body weight gain, and aflatoxin only affect feed intake of 21-day-old birds. No interaction was observed between aflatoxin and ochratoxin at the levels used in experimental study. Yeast cell wall did not significantly reduced the deleterious effects of ochratoxins. No significant differences were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Ochratoxin; Yeast cell wall; Broilers; Performance.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000400004
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Fungos associados a Hypothenemus hampei Ferrari (Coleoptera, Scolytidae) e aos frutos de Coffea canephora em Machadinho D'Oeste, RO. Repositório Alice
GAMA, F. de C.; TEIXEIRA, C. A. D.; VIEIRA, R. X.; COSTA, J. N. M.; GARCIA, A..
2004
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Interações entre espécies; Dispersão de espécies; Ocratoxina; Species interactions; Species dispersion; Ochratoxin.
Ano: 2004 URL: http://www.alice.cnptia.embrapa.br/handle/doc/710613
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Molecular identification of Aspergillus spp. isolated from coffee beans Scientia Agricola
Magnani,Marciane; Fernandes,Thiago; Prete,Cássio Egidio Cavenaghi; Homechim,Martin; Ono,Elisabete Yurie Sataque; Vilas-Boas,Laurival Antonio; Sartori,Daniele; Furlaneto,Márcia Cristina; Fungaro,Maria Helena Pelegrinelli.
Some species belonging to the genus Aspergillus are potential producers of ochratoxin A (OA), a mycotoxin with nephrotoxic, immunosuppressive, teratogenic and carcinogenic effects. The aim of the present study was to identify the species of Aspergillus that contaminate the inside of coffee beans collected in the stage of maturation and drying, from 16 producing areas located in the northern region of the State of Paraná, in the South of Brazil. A total of 108 isolates of Aspergillus spp. was identified at the species level, by sequencing the internal transcribed spacer (ITS1-5.8S-ITS2) of ribosomal DNA (rDNA). The results revealed the presence of potentially ochratoxigenic species in 82% of the geographic regions studied, among which Aspergillus niger was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: ITS; Ribosomal DNA; Ochratoxin; Mycotoxin.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000100009
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Parboiled rice: chemical composition and the occurrence of mycotoxins Ciênc. Tecnol. Aliment.
Dors,Giniani Carla; Bierhals,Vânia da Silva; Badiale-Furlong,Eliana.
The objective of this work was to evaluate the incidence of aflatoxin B1 (AFB B1), deoxynivalenol (DON), ochratoxin A (OTA), and zearalenone (ZEA) in parboiled rice with respect to its chemical composition. Eight lots from five different brands of parboiled rice were collected in four samplings, at different seasons, until the amount of 32 lots. It was observed that: DON was present in 22% of the samples (from 180 to 400 ppb); ZEA in 19% (from 317 to 396 ppb); OTA in 12.5% (from 13 and 26 ppb); and AFB B1 in 9% (from 11 to 74 ppb). The results of the chemical composition were not different from those previously mentioned in the literature concerning parboiled rice. The ash and phenol levels in the contaminated parboiled rice samples suggested that those...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Deoxynivalenol; Ochratoxin; Physical-chemical characterization; Zearalenone.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000100025
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Survey of mycotoxins in Southern Brazilian wheat and evaluation of immunoassay methods Scientia Agricola
Duffeck,Maíra Rodrigues; Tibola,Casiane Salete; Guarienti,Eliana Maria; Del Ponte,Emerson Medeiros.
ABSTRACT One hundred commercial wheat grain samples were collected during the 2015 sea-son across 78 municipalities in the states of Paraná (PR), Rio Grande do Sul (RS), and São Paulo (SP), Brazil. Separate subsamples were analyzed for the concentration of deoxynivalenol (DON), zearalenona (ZEA) and ochratoxin A (OTA) mycotoxins using two methods: UHPLC-MS/MS (reference method) and a commercial enzyme-linked immunosorbent assay (ELISA) (AgraQuant®). The OTA mycotoxin was not found in the samples by both methods. DON and ZEA were detected in 55 % and 39 % of the samples by the reference method, with overall mean levels of 795.2 μg kg−1 and 79.78 μg kg−1, respectively. There was a significant and positive correlation (Spearman rank) between DON and ZEA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum; UHPLC; ELISA; Trichothecenes; Ochratoxin.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162017000400343
Registros recuperados: 6
Primeira ... 1 ... Última
 

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