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Development of transformation vectors for the production of potentially high oleate transgenic oil palm Electron. J. Biotechnol.
Masani,Mat Yunus Abdul; Parveez,Ghulam Kadir Ahmad.
The main target of Malaysian Palm Oil Board (MPOB) genetic engineering programme is to produce high oleate transgenic palms. The availability of effective transformation vector is one of the pre-requisites for genetic manipulation of oil palm through recombinant DNA technology. Here, we describe the construction of a series of transformation vectors that have a maize ubiquitin promoter (UbiPro)-driven bar gene for selection of transformants on herbicide (Basta or Bialaphos), and mesocarp-specific promoter (MSP1) for expression of the transgenes [antisense palmitoyl-ACP-thioesterase (PAT) and sense β-ketoacyl-ACP-synthase II (KASII) and sense Δ9-stearoyl-ACP-desaturase (SAD)] potentially responsible for high oleate content in oil palm...
Tipo: Journal article Palavras-chave: Oleate; Transformation vectors; Transgenic oil palm.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300003
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Sodium azide mutagenesis resulted in a peanut plant with elevated oleate content Electron. J. Biotechnol.
Wang,Chuan Tang; Tang,Yue Yi; Wang,Xiu Zhen; Zhang,Shu Wei; Li,Gui Jie; Zhang,Jian Cheng; Yu,Shan Lin.
Screening of peanut seeds resulting from 0.39% sodium azide treatment with NIRS calibration equation for bulk seed samples identified a plant with more than 60% oleate. Oleate content in individual seeds of the plant, as predicted by NIRS calibration equation for intact single peanut seeds, ranged from 50.05% ~ 68.69%. Three seeds with >60% oleate thus identified were further confirmed by gas chromatography. Multiple sequence alignments of the FAD2B gene from Huayu 22 (wild type) and peanut seeds with elevated oleate (mutant type) revealed a C281T transition in the coding region causing an I94T substitution in the oleoyl-PC desaturase, which may be responsible for reduction in the enzyme activity.
Tipo: Journal article Palavras-chave: Chemical mutant; FAD2B; NIRS; Oleate; Peanut.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200010
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