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Assisted reproductive techniques in small animals MV&Z
Lopes, Maria Denise.
The objective of this discussion is to bring information about some of the assisted reproductive techniques used in domestic animals, and the impact of this technology on reproduction of dogs and cats.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spermatozoa; Oocytes; Fertilization; Reproductive techniques espermatozóide; Oócito; Fertilização; Técnicas reprodutivas; Cão; Gato.
Ano: 2001 URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3341
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Biologia reproductiva de la saraquita Ramnogaster arcuata (Jenyns, 1842) del estuario del Rio de la Plata. OceanDocs
Rodrigues, K.A..
The reproductive biology of Ramnogaster arcuata inhabiting waters of the Rio de la Plata estuary (Argentina - Uruguay) was studied by using macroscopic and histological analysis of ovaries. R. Arcuata specimens were collected from two research cruises of the National Institute of Fisheries Research and Development (INIDEP) carried out in June 1998 and June 1999. During these surveys, bottom salinity and temperature data were also registered. In addition, information about annual catches of this species in the Rio de la Plata, obtained from different INIDEP cruises performed during the last ten years, was analyzed. During winter, a prevalence of R. arcuata individuals was observed in the Rio de la Plata estuary. In summer, this species apparently moves...
Tipo: Theses and Dissertations Palavras-chave: Sexual reproduction; Ovaries; Fecundity; Spawning seasons; Histology; Fish eggs; Oocytes; Environmental conditions; Ovaries; Sexual reproduction; Fecundity; Oocytes; Environmental conditions; Http://aims.fao.org/aos/agrovoc/c_5456; Http://aims.fao.org/aos/agrovoc/c_26876; Http://aims.fao.org/aos/agrovoc/c_15725; Http://aims.fao.org/aos/agrovoc/c_29343; Http://aims.fao.org/aos/agrovoc/c_32980.
Ano: 2005 URL: http://hdl.handle.net/1834/1485
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Comparative Studies on lipid Analysis and Ultrastructure in Porcine and Southern Minke Whale (Balaenoptera Bonaerensis) Oocytes OAK
FUJlHIRA, Takuma; KINOSHITA, Mikio; SASAKI, Motoki; OHNISHI, Masao; ISHIKAWA, Hajime; OHSUMI, Seiji; FUKUI, Yutaka; 木下, 幹朗; 佐々木, 基樹; 大西, 正男.
The present study was conducted to clarify the difference in the color of the cytoplasm in immature follicular oocytes from prepubertal and adult minke whales. The four lipid contents (triglyceride,total cholesterol phospholipids and non-esterified fatty acids) in vitrified immature oocytes from prepubertal and adult minke whales,and also in fresh and vitrified immature porcine oocytes,were measured. The lipid contents in vitrified-warmed minke whale oocytes were similarly high compared with those in vitrified-warmedp orcine oocytes. In particular,the total cholesterol and phospholipid contents in the vitrified immature oocytes from prepubertal and adult minke whales were significantly( P<0.05) higher than those from prepubertal pigs. Furthermore,the...
Palavras-chave: Minke whale; Porcine; Oocytes; Lipid; Sexual maturity.
Ano: 2004 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2731
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Contrasting effects of heat shock during in vitro maturation on development of in vitro-fertilized and parthenogenetic bovine embryos. Repositório Alice
CAMARGO, L. S. A.; COSTA, F. Q.; MUNK, M.; WOHLRES-VIANA, S.; SERAPIÃO, R. V.; CARVALHO, B. C.; CAMPOS JÚNIOR, P. H.; VIEIRA, A. C.; NOGUEIRA, L. A. G.; VIANA, J. H. M..
bitstream/item/203452/1/Camargo-et-al-2019-Reproduction-in-Domestic-Animals.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Activation; Embryos; Maturation; Aquaporin 3; Hsp70 Protein; Ionomycin; Heat Shock; Cleavage; Blastocyst; In vitro fertilization; Parthenogenesis; Apoptosis; Gene expression; Gametocytes; Oocytes.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113387
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Contrasting effects of heat shock during in vitro maturation on development of in vitro-fertilized and parthenogenetic bovine embryos. Repositório Alice
CAMARGO, L. S. A.; COSTA, F. Q.; MUNK, M.; WOHLRES-VIANA, S.; SERAPIÃO, R. V.; CARVALHO, B. C.; CAMPOS JÚNIOR, P. H.; VIEIRA, A. C.; NOGUEIRA, L. A. G.; VIANA, J. H. M..
Abstract This study investigated the influence of heat shock during in vitro maturation on embryo development following in vitro fertilization (IVF) or parthenogenesis (Part). Immature bovine cumulus?oocyte complexes were exposed to heat shock (41.0°C) during the first 12 hr of in vitro maturation (IVM), followed by 12 hr at 38.5°C. Control group consisted of in vitro maturation for 24 hr at 38.5°C. Oocytes were in vitro&#8208;fertilized or activated with ionomycin and cultured in vitro for 192 hr post&#8208;in vitro insemination or parthenogenetic activation (hpia). There was an interaction (p < .01) between temperature of IVM and method of oocyte activation (IVF or Part) for cleav&#8208;age at 48 hpia. Heat shock had a negative impact (p...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Activation; Embryos; Maturation; Aquaporin 3; Hsp70 Protein; Ionomycin; Heat Shock; Cleavage; Blastocyst; In vitro fertilization; Parthenogenesis; Apoptosis; Gene expression; Gametocytes; Oocytes.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1118469
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Different progestagens sources do not affect the follicular population and the morphological quality of oocytes during ovarian stimulation in Santa Inês ewes. Repositório Alice
BRAGANÇA, G. M.; SOUZA-FABJAN, J. M. G.; BATISTA, R. I. T. P.; RIBEIRO, L. S.; BRAIR, V. L.; CÔRTES, L. R.; SOUZA, C. V.; TRAIRA, A. R.; FONSECA, J. F. da; MENCHACA, A.; BRANDÃO, F. Z..
In attempt to develop a hormonal protocol more suitable to produce good quality oocytes for use in biotechnologies, an earlier study demonstrated that FSH applied in multiple decreasing doses produced better quality oocytes. However, different progestagens implants used during ovarian stimulation have not yet been tested. The aim of this study was to investigate the effect of different progestagens during ovarian stimulation on follicular population and oocyte morphological quality in Santa Inês ewes. In conclusion, the source of progestagens used during the ovarian stimulation protocol does not affect the follicular population, nor the oocyte quality. Exogenous progestagens may not be necessary when postsynchronization ovulation is confirmed by...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Animal reproduction; Sheep; Ewes; Oocytes.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1095105
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Donnees cytologiques, electrophysiologiques et biochimiques relatives au phenomene de fecondation chez le homard (Homarus gammarus ). Implication du processus d'activation sur le mecanisme d'attache des oeufs pondus ArchiMer
Goudeau, M; Lachaise, F; Kubisz, P; Goudeau, H.
Electrophysiological measurements and related cytological controls were done on immature and submature oocytes of Homarus gammarus . By chromatography (HPLC) and radio-immunoassays (RIA) it was established that submature oocytes displayed different ecdysteroids. Furthermore, the structure of the stalk (funiculus) for the laid eggs attachment was analyzed. it consisted of the two superimposed vitelline envelopes which were likely modified by the cortical reaction exudate during the fertilization process. In addition, the successive secretory phases, regarding to the embryonic envelopes were evidenced. Finally, the ecdysteroid titers of embryonic stages were measured.
Tipo: Text Palavras-chave: Homarus gammarus; Malacostraca; Biological attachment; Hormones; Electrophysiology; Biochemistry; Cytology; Eggs; Embryonic development; Cell membranes; Oocytes; Fecundity; Homard; Decapode; Crustacé; Funicule; Mues embryonnaires; Enveloppe de fécondation; Ecdystéroîdes; Potentiel membranaire; Ovocyte.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1190.pdf
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Efeitos da redução ou substituição do soro fetal bovino por outros compostos na maturação in vitro de oócitos bovinos. Repositório Alice
DEL COLLADO, M.; SARAIVA, N. Z.; LOPES, F. L.; CRUZ, M. H.; GASPAR, R. C.; OLIVEIRA, C. S.; PERECIN, F.; GARCIA, J. M..
A utilização do soro fetal bovino (SFB), embora bastante disseminada na produção in vitro (PIV) de embriões bovinos, apresenta limitações por ser um meio indefinido e por causar efeitos que prejudicam a qualidade desses embriões. Por esse motivo, nos últimos anos, grande parte das pesquisas relacionadas à PIV está voltada para a substituição do SFB por outros compostos nos meios de cultura. No presente estudo, foram utilizados como compostos protéicos a albumina sérica bovina livre de ácidos graxos (BSA-FAF) e um produto comercial denominado fluido embriônico (FE) de maneira isolada ou em diferentes combinações e concentrações, com objetivo de substituir ou diminuir a concentração do SFB durante a maturação in vitro (MIV). Para isso, oócitos bovinos foram...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Oócitos bovinos; Maturação in vitro; Soro fetal bovino; Oocytes; In vitro maturation; Fetal calf serum; Cattle.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1003939
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Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro. Repositório Alice
CRUZ, M. H. C.; SARAIVA, N. Z.; CRUZ, J. F. da; OLIVEIRA, C. S.; COLLADO, M. del; FERNANDES, H.; CASTRO, F. C. de; GARCIA, J. M..
2014
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Embryo development; Inner cell mass; In vitro fertilization; Oocytes.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1010500
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Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro R. Bras. Zootec.
Cruz,Maria Helena Coelho; Saraiva,Naiara Zoccal; Cruz,Jurandir Ferreira da; Oliveira,Clara Slade; Del Collado,Maite; Fernandes,Hugo; Castro,Fernanda Cavallari de; Garcia,Joaquim Mansano.
This study evaluated the influence of follicular fluid (FF) added to the maturation medium on the quality of bovine embryos produced in vitro. In the first experiment, oocytes were matured in media containing five different FF concentrations with different maturation times and classified according to meiotic progression and migration of cortical granules. In the second experiment, oocytes matured in the same media were fertilized at three different maturation times; thereafter, cleavage and blastocyst rates were evaluated. In the third experiment, oocytes were matured in media containing three different FF concentrations at two different maturation times, and embryo quality, inferred by the ratio of inner cell mass and trophectoderm cells compared with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Embryo development; Inner cell mass; In vitro fertilization; Oocytes.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000300003
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Effect of two ram sperm capacitating media on acrosome reaction and zona-free hamster oocyte penetration test Braz. J. Vet. Res. Anim. Sci.
FERRARI,Silvia; BARNABE,Valquíria Hyppolito; ZÜGE,Roberta Mara; ZOGNO,Maria Amélia.
An in vitro zona-free hamster oocyte penetration test was utilized in 24 trials in order to evaluate the capacitation media used for ram sperm. A pool of fresh semen was collected from three crossed breed rams. Two semen drops were washed by centrifugation and incubated in high ionic strength treatment (HIS) or in a defined medium with HEPES, on heat ewe serum and heparin. After the incubation to promote capacitation, simplified triple-stain technique was used to evaluate the spontaneous acrosome reaction of the capacitated sperm. Superovulation in 96 golden hamsters was induced by PMSG and hCG. The oocytes were treated with hyaluronidase and trypsin to remove, respectively, the cumulus cells and the zona pellucida. Oocytes and capacitated sperm were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Capacitation; Spermatozoa; Rams; Oocytes; Hamsters.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000300010
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Follicular fluid concentration and oocyte quality from Toggenburg goats fed with urea. Repositório Alice
AMORIM, E. A. M.; AMORIM, L. S.; TORRES, C. A. A.; GUIMARÃES, J. D.; FONSECA, J. F. da; SEIDEL JÚNIOR, G. E..
2009
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Fluido folicular; Caprino; Raça Toggenburg; Reprodução animal; Endocrinologia; Ovário; Cabra; Oocytes; Goats.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/handle/doc/749006
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Histological characterization of cellular types during Scinax fuscovarius oogenesis (Lutz) (Anura, Hylidae) Zoologia
Oliveira,Classius de; Santos,Lia Raquel de Souza.
This paper describes morphological characteristics of the ovarian germinative cells of the Scinax fuscovarius (Lutz, 1925). The ovary is organized in primordial germinative cells (oogonia) and follicular structures (ovarian follicle) - oocytes surrounded by follicular cells. Oogonia: their nests are peripherically localized, containing cells with large and oval nucleus. Oocytes I: basophilic cytoplasm; the spherical nucleus presents few nucleoli, one or two; the follicular cells (one tenuous layer) surround these previtellogenic cells. Oocytes II: the cell is larger and the cytoplasm becomes more basophilic; the nucleus presents few nucleoli and contains many chromosomes in the periphery (beginning the perinucleolar stage). Oocytes III: the cytoplasm...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oocytes; Oogonia; Ovary; Pigment; Vitellogenesis.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752004000400029
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In vitro embryos production in bovine after metaphase plate nuclear transfer Repositório Alice
CAIXETA, F. M. C.; SOUSA, R. V. de; SPRÍCIGO, J. F. W.; RODRIGUES, S. de A. D.; FIDELIS, A. A.; DODE, M. A. N..
bitstream/item/171187/1/SBTExAETE-v14n320171-439-439.pdf
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Bovine; Oocytes.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1085574
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Influence of climatic conditions on in vitro production of bovine embryos Arq. Bras. Med. Vet. Zootec.
Pessoa,G.A; Navarro,R.B; Rauber,L.P; Brass,K.E; Silva,C.A.M; Rubin,M.I.B.
Temperature and rainfall were analyzed daily during six years to evaluate their influence on in vitro production of bovine embryos. Weekly replications (n=480) were performed on 14,778 ovaries collected at slaughterhouses. Cumulus oocyte complexes (n=19,180) were fertilized with a pool of Bos taurus taurus semen in one incubator with 5% CO2. Presumable zygotes were cultured in gasified plastic bags with 5% CO2, 5% O2, and 90% N2. In the first year, cleavage and embryo yield were 60.3% and 15.6%, respectively, being lower (P<0.05) than in the following years. Average cleavage rates were always lower in winter (P<0.0001), thus producing less embryos. Winter climatic conditions had a negative influence on in vitro production, when cleavage and embryo...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cow; Oocytes; Embryo; Climate; In vitro prodution.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600013
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Inseminating dose and water volume applied to the artificial fertilization of Steindachneridion parahybae (Steindachner, 1877) (Siluriformes: Pimelodidae): Brazilian endangered fish Neotropical Ichthyology
Sanches,Eduardo Antônio; Caneppele,Danilo; Okawara,Renan Yoshiharu; Damasceno,Danielle Zanerato; Bombardelli,Robie Allan; Romagosa,Elizabeth.
Abstract The Steindachneridion parahybae is an endangered catfish from Brazil and strategies applied for gametes optimization are necessary. The aim of this study was to assess inseminating doses and water volume upon the fertilization, hatching rates and percentage of normal larvae in S. parahybae . Was used a randomized design in factorial scheme (4×4) with four inseminating doses: 1.0×104, 1.0×105, 1.0×106, 1.0×107spermatozoa oocyte-1 and four volumes of water: 1, 35, 65 and 95mL of water g-1 of oocytes. The combination of doses and volumes were performed in triplicates (n=48). Each incubator (1.5L of useful volume) with 1g of oocytes was considered as an experimental unit. Significant interaction between inseminating doses and volumes of water to the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Artificial reproduction; Freshwater fish; Oocytes; Sperm; Surubim-do-Paraíba.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000100204
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Main aspeets of the manipulation of ooeytes enelosed in preantral ovarian follieles MV&Z
Max, Marilu Constantino; Andrade, Evelyn Rabelo de; Basso, Andrea Cristina; de Figueiredo, José Ricardo; Seneda, Marcelo Marcondes.
Objective: To disclose a new biotechnique, listing some of its aspects and addressing its main stages, isolation, cryopreservation and culture of preantral follicles. Data Sources: The information was obtained from CAB, MEDLINE, PUBMED, Science Direct and CAPES Portal. Data Synthesis: The preantral follicles store about 90% of the oocytes within the ovary. The biotechnique described is based on recovering immature oocytes retained inside preantral follicles before atresia takes place. The cryopreservation of these cells enables the creation of germoplasm banks, preserving the genetic material of women submitted to chemotherapy and/or radiotherapy, as well as that endangered animal species. Conclusions: Being a recent biotechnique, its application is...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotechonology; Reproductive techniques; Oocytes; Transplantation; Ovarianfollicles; Cryopreservation Biotecnologia; Técnicas reproductivas; Oocitos; Transplantación; Foliculo ovárico; Criopreservación biotecnologia; Técnicas reprodutivas; Oócitos; Transplante; Folículo ovariano; Criopreservação.
Ano: 2004 URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3239
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Molecular Characterization, Electrophysiological and Contraceptive Effect of Chilean Latrodectus Venom International Journal of Morphology
Navarrete Gómez,Patricia; Ormeño,David; Miranda,Antonio; Sánchez Gutiérrez,Raúl; Romero Mejía,Fernando; Parodi Rivera,Jorge.
Since the 1970s, There have been studies of the venom of Latrodectus sp. spiders, in particular the latrotoxin (LTX) of Latrodectus mactans. Many of the studies were aimed at understanding the action of the venom on the muscular system. Now accepted that LTX is able to generate a calcium-permeable membrane pore and modulate the release of synaptic vesicles that activate a receptor and induce cellular changes. Interestingly, when work began with venom obtained from the Latrodectus sp present in Chile, it generated clinical indications similar to the bite of this spider in another country, with some differences in intensity. The purpose of the first studies was to understand the systemic mechanisms of this venom, and other active compounds were studied for...
Tipo: Journal article Palavras-chave: Latrodectus venom; Oocytes; Neurons; Spermatozoa; Contraceptive.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300012
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Ovarian Grafts 10 days after Xenotransplantation: folliculogenesis and recovery of viable oocytes. Repositório Alice
CAMPOS-JUNIOR, P. H. A.; ALVES, T. J. M.; DIAS, M. T.; ASSUNÇAO, C. M.; MUNK, M.; MATTOS, M. S.; KRAEMER, L. R.; ALMEIDA, B. G.; RUSSO, R. C.; BARCELOS, L.; CAMARGO, L. S. de A.; VIANA, J. H. M..
Abstracts Ovarian xenotransplantation is a promising alternative to preserve fertility of oncologic patients. However, several functional aspects of this procedure remained to be addressed. The aim of this study was evaluate the feasibility of xenotransplantation as a strategy to maintain bovine ovarian grafts and produce oocytes. Adult ovarian cortical pieces were xenotransplanted to the dorsal subcutaneous of female NOD-SCID mice (n = 62). Grafts were recovered ten days after xenotransplantation. Host and graft weights; folliculogenesis progression; blood perfusion, relative gene expression and number of macrophage and neutrophil of xenografts; in vitro developmental competence of graft-derived oocytes were evaluated. Folliculogenesis was supported in...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Bovine ovarian grafts; Oocytes; Ovarian xenografts.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1062986
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Ovarian histology and fecundity in the evaluation of the reproduction of the invasive species Serrasalmus marginatus (Characidae) on a neotropical floodplain Biological Sciences
Melo, Gabriele Sauthier Romano de; Santana, Herick Soares de; Tos, Claudenice Dei.
 The construction of the Itaipu Hydroelectric Power Plant in 1982 led to the formation of a reservoir, which, in turn, leveled the waters of the Paraná River by flooding the geographic barrier Salto de Sete Quedas. This allowed the piranha Serrasalmus marginatus to invade and colonize the upper Paraná River. This study aimed to: i) confirm, through light microscopy, the reproductive phases of S. marginatus females; ii) estimate fecundity and iii) evaluate the reproduction areas of the population. A total of 764 females were collected from nine sampling sites on the upper Paraná River floodplain. Microscopic analysis of the ovaries showed the following phases: early developing subphase, developing phase, spawning capable phase, actively spawning subphase,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.05.02.00-1 Indeterminate fecundity; Type of spawning; Oogenesis; Oocytes; Piranha. Ecologia de Ecossistemas.
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/33021
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