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Registros recuperados: 37
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Adsorption of norovirus and ostreid herpesvirus type 1 to polymer membranes for the development of passive samplers ArchiMer
Hubert, Francoise; Morga, Benjamin; Renault, Tristan; Le Guyader, Soizick.
Aims This study was performed to develop passive sampling methodology for the detection of two viruses in seawater in the area of shellfish production, The Norovirus (NoV), a human pathogen implicated in gastroenteritis outbreaks linked to oyster consumption and the ostreid herpesvirus type 1 (OsHV-1) a virus associated with mass mortalities of Pacific oysters. Methods and Results Commercially membranes were tested for their capacity to adsorb virus: Zetapor, gauze, nylon, low density polyethylene (LDPE) and Polyvinylidene difluoride (PVDF). Laboratory exposures of membranes to contaminated water samples (stool, sewage, seawater) were performed. Our data shown that the amount of NoV GII genome per membrane measured with qRT-PCR increased with the time of...
Tipo: Text Palavras-chave: Norovirus; OsHV-1; Pacific oysters; Passive samplers; Seawater; Sewage.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00365/47646/47670.pdf
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Approche moléculaire des effets de polluants sur l'infection à virus OsHV-1 (Ostreid Herpesvirus 1) chez l'huître creuse, Crassostrea gigas ArchiMer
Moreau, Kevin.
Des mortalités chez l'huître creuse, Crassostrea gigas, ont été associées au virus OsHV-1 (Oyster herpes virus 1) au stade larvaire et chez des juvéniles. Cependant, aucune mortalité liée à ce virus n'a été rapportée chez les animaux adultes. Cette observation laisse suspecter l'existence de mécanismes dirigés contre ce virus chez cette espèce. Il a peu de connaissances à l'heure actuelle sur les mécanismes de défense antivirale chez les bivalves, en particulier chez l'huître creuse, C. gigas, comme étant sur-exprimés en présence du virus. Ces gènes, s'ils ne peuvent être considérés comme des gènes de défense à proprement parlé, participent à la réponse de l'huître vis à vis du virus. Par ailleurs, des polluants comme les pesticides sont susceptibles de...
Tipo: Text Palavras-chave: Crassostrea gigas; OsHV-1; Pesticides; Expression génétique; RT-PCR semi-quantative.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/00032/14339/11622.pdf
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Associations between farming practices and disease mortality of Pacific oyster Crassostrea gigas in a Mediterranean lagoon ArchiMer
Pernet, Fabrice; Lagarde, Franck; Le Gall, Patrik; Roque D'Orbcastel, Emmanuelle.
We present the first large-scale, high-resolution spatial and temporal pattern of disease mortality caused by Ostreid herpesvirus 1 (OsHV-1) that has affected Pacific oyster Crassostrea gigas in France every year since 2008, in relation to local farm use. Mortality was monitored in healthy oyster spat deployed at 106 locations in the Thau Mediterranean lagoon during the epizootic that occurred in spring 2011. We conducted a field survey of farming practices in the areas where the sentinel oysters were placed before the epizootic. We found that the risk of disease mortality in sentinel oysters was higher within the bivalve farming area than outside it, suggesting that the infection pressure is much higher in areas with intensive farming activity. The risk...
Tipo: Text Palavras-chave: Aquaculture; Epidemiology; OsHV-1; Shellfish farming.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00201/31248/30662.pdf
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Autophagy in Pacific oyster, Crassostrea gigas ArchiMer
Moreau, Pierrick; Moreau, Kevin; Morga, Benjamin; Tourbiez, Delphine; Travers, Marie-agnes; Rubinsztein, David; Renault, Tristan.
Mass mortality outbreaks of Pacific oysters, Crassostrea gigas, are reported in different areas around the world affecting seriously the shellfish aquaculture sector. Two pathogens are associated with these mortality outbreaks, ostreid herpesvirus 1 (OsHV-1) and Vibrio aestuarianus. In this contexte, a better knowledge is needed in terms of oyster immunity. For this purpose, as an important degradation pathway autophagy was investigated in the Pacific oysters. An in silico research of genes involved in autophagy using the C. gigas genome allowed identification of homologs of ATG1 and ATG8/LC3(Microtubule-associated protein 1A/1B-light chain 3). Gene expression and protein detection were analysed using real-time PCR and western blot, respectively. ATG1 and...
Tipo: Text Palavras-chave: Autophagy; Pacific oyster; Crassostrea gigas; OsHV-1; Vibrio aestuarianus.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00218/32876/31345.pdf
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Autophagy plays an important role in protecting Pacific oysters from OsHV-1 and Vibrio aestuarianus infections ArchiMer
Moreau, Pierrick; Moreau, Kevin; Segarra, Amelie; Tourbiez, Delphine; Travers, Marie-agnes; Rubinsztein, David C.; Renault, Tristan.
Recent mass mortality outbreaks around the world in Pacific oysters, Crassostrea gigas, have seriously affected the aquaculture economy. Although the causes for these mortality outbreaks appear complex, infectious agents are involved. Two pathogens are associated with mass mortality outbreaks, the virus ostreid herpesvirus 1 (OsHV-1) and the bacterium Vibrio aestuarianus. Here we describe the interactions between these 2 pathogens and autophagy, a conserved intracellular pathway playing a key role in innate immunity. We show for the first time that autophagy pathway is present and functional in Pacific oysters and plays an important role to protect animals from infections. This study contributes to better understand the innate immune system of Pacific...
Tipo: Text Palavras-chave: Autophagy; Crassostrea gigas; OsHV-1; Pacific oyster; Vibrio aestuarianus; ATG; Autophagy-related; Atg8-PE; Atg8-phosphatidylethenolamine; DNA; Deoxyribonucleic acid; Hpi; Hours postinfection; LC3-II; Cleaved; Lipidated and autophagosome-associated form of LC3; MAP1LC3A; B (LC3A; B); Microtubule-associated proteins 1 light chain 3 alpha.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00269/37989/36072.pdf
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Complete genome sequence of acute viral necrosis virus associated with massive mortality outbreaks in the Chinese scallop, Chlamys farreri ArchiMer
Ren, Weicheng; Chen, Haixia; Renault, Tristan; Cai, Yuyong; Bai, Changming; Wang, Chongming; Huang, Jie.
Acute viral necrosis virus (AVNV) is the causative agent of a serious disease resulting in high mortality in cultured Chinese scallops, Chlamys farreri. We have sequenced and analyzed the complete genome of AVNV. The AVNV genome is a linear, double-stranded DNA molecule of 210,993 bp with a nucleotide composition of 38.5% G + C. A total of 123 open reading frames were predicted to encode functional proteins, ranging from 41 to 1,878 amino acid residues. The DNA sequence of AVNV is 97% identical to that of ostreid herpesvirus 1 (OsHV-1), and the amino acid sequences of the encoded proteins of these two viruses are 94-100% identical. The genomic organization of AVNV is similar to that of OsHV-1, and consists of two unique regions (170.4 kb and 3.4 kb,...
Tipo: Text Palavras-chave: Acute viral necrosis virus; Herpesvirus; OsHV-1; Genome; Scallop; Chlamys farreri; AVNV.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00135/24620/22650.pdf
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Désinfection de l’eau de mer – DESIMER Etude des sous-produits de désinfection ArchiMer
Stavrakakis, Christophe; Papin, Mathias; Dupuy, Beatrice; Riou, Karen; Penisson, Christian; Nourry, Max; Hatt, Philippe-jacques; Buchet, Vincent; Palvadeau, Hubert.
L'eau qui alimente des structures d’élevage ou encore des plateformes expérimentales aquacoles (et potentiellement l’eau qui en est issue) peut être contaminée par des agents pathogènes infectant les coquillages, et plus particulièrement les huîtres, ou encore par des agents pathogènes entériques représentant un risque pour les consommateurs. Par ailleurs, la production possible d'huîtres tétraploïdes dans les installations d’élevage implique l’étude de solutions de traitement adaptées pour éviter tout rejet de gamètes, larves ou naissain d’animaux polyploïdes dans l'environnement. Le projet DESIMER a donc pour objectif d’étudier et de comparer différentes techniques de désinfection de l’eau de mer : la chloration, l’ozonation et le rayonnement UV afin de...
Tipo: Text Palavras-chave: Désinfection; Traitement de l’eau; Sous-produits; Vibrio aestuarianus; OsHV-1; Crassostrea gigas.; Disinfection; Water Treatment; By-products; Vibrio aestuarianus; OsHV-1; Crassostrea gigas..
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00376/48756/49141.pdf
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Diagnosis of oyster herpes-like virus: development and validation of molecular, immunological and cellular tools "VINO" - Final Report 4th January 1999 to 3rd January 2002 ArchiMer
Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
Little information is available on viral infections that affect bivalve molluscs. Such a lack of data is due ta a certain inadequacy of the diagnosis methods that are employed when massive mortality events occur. Most laboratories involved in mollusc pathology still analyse samples through light microscopy. World-wide, there is thus currently a lack of information concerning the occurrence of bivalve herpesvituses. This is probably due to the lack of suitable diagnostic tools. The basic method for identification and examination of suspect samples is predominantly histopathology. This enables the identification of any cellular changes, but is not conclusive identification of bivalve herpesviruses. This technique doesn't allow, by itself, to detect viruses...
Tipo: Text Palavras-chave: Pathology; Virology; Diagnosis; Herpes like virus; Validation molecular; Detection; Herpesviridae; OsHV-1; Diagnostic techniques; Oysters.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00044/15525/12912.pdf
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Diagnosis of oyster herpes-like virus: development and validation of molecular immunological and cellular tools "VINO". Second periodic progress report: 4th January 2000 at 3rd January 2001 ArchiMer
Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
The aim of the programme is to develop specific tools for diagnosing herpes-like virus infections in bivalves and to validate these reagents by using them in different European laboratories involved in shellfish epidemiological surveys. This will done using teclmiques developed by the patiners to characterise viruses by studying their genome and immunologically reactive proteins, to cultivate oyster cells and vertebrate cell lines and to perform epidemiological surveys among bivalves. The programme objectives are: 1 - Obtaining the complete oyster herpesvirus (OsHV-1) DNA sequence with determination of the genome structure. 2 - Comparing OsHV -1 with viruses belonging to the Herpesviridae family on the basis of sequence data and genome structure. 3 -...
Tipo: Text Palavras-chave: Pathology; Virology; Diagnosis; Herpes like virus; Validation molecular; Detection; Herpesviridae; OsHV-1; Diagnostic techniques; Oysters.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/00048/15947/13377.pdf
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Diagnosis of oyster herpes-like virus: development and validation of molecular, immunological and cellular tools "VINO" Third Periodic Progress Report : 4th January 2001 to 3rd January 2002 ArchiMer
Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
Herpesvirus infections have been reported in different bivalves species around the world. Specific diagnostic tools are needed in order ta assess the causative raIe of these agents in mortalities observed among bivalves There is currently a lack of information concerning the occurrence of bivalve herpesviruses. This is probably due ta the lack of suitable diagnostic tools. The basic method for examination of bivalve samples is predominantly histology. This enables the identification of any cellular changes, but is not conclusive for virus identification. Transmission electron microscopy is a necessity for visual confirmation of viral infections. However, histology and electron microscopy are time consuming and inadequate for epidemiological studies. The...
Tipo: Text Palavras-chave: Pathology; Virology; Diagnosis; Herpes like virus; Virus; Oyster; Detection; OsHV-1; Herpesviridae.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00044/15524/12911.pdf
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Double résistance aux infections dues aux organismes pathogènes Vibrio aestuarianus et OsHV-1 chez l’huître creuse Crassostrea gigas – DORE. Rapport scientifique final de l’étude DORE, convention DPMA-Ifremer 2016 ArchiMer
Degremont, Lionel; Maurouard, Elise; Godfrin, Yoann; Travers, Marie-agnes; Morga, Benjamin; Lamy, Jean-baptiste; Benabdelmouna, Abdellah.
Depuis 2012, le nombre de cas de mortalités d’huîtres creuses adultes (Crassostrea gigas) rapportés dans le cadre des réseaux de surveillance de l’Ifremer, et dans lesquels la bactérie Vibrio aestuarianus a été isolée, a fortement augmenté. Les objectifs de cette étude étaient (1) d’étudier la sensibilité des huîtres C. gigas à une infection à V. aestuarianus pour des lots diploïdes et triploïdes et (2) déterminer la réponse à la sélection pour une meilleure résistance à V. aestuarianus pour des huîtres C. gigas. Neuf stocks ont été utilisés, répartis en trois niveaux de sélection, chacun étant donc répliqué par trois pontes. Pour chaque stock, un lot diploïde (2n), un lot triploïde (3n_chim) produit par la rétention d’un globule polaire, et trois lots...
Tipo: Text Palavras-chave: Vibrio aestuarianus; OsHV-1; Sélection; Résistance; Triploïdes; Huître creuse; Crassostrea gigas; Vibrio aestuarianus; OsHV-1; Selective breeding; Disease resistance Pacific oyster; Crassostrea gigas; Triploids.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00404/51530/52140.pdf
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Étude des interactions entre infection à ostreid herpesvirus 1, immunité, autophagie et pesticides chez l’Huître creuse, Crassostrea gigas ArchiMer
Moreau, Pierrick.
The thesis work is part of the very current issue on mass mortality outbreaks affecting Pacific oyster, C. gigas, spat and juveniles and questions about the involvement of pesticides in this phenomenon. The first part of this thesis was devoted to study the effects of pesticides on hemocyte parameters in the Pacific oyster (in vitro and in vivo). The immunomodulator effect of selected pesticides (alone or in mixture) has been explored principally through flow cytometry. The second part concerned the study of the effects of a pesticides mixture on OsHV-1 itself. No direct effects have been reported on the viral particles in presence of the pollutants. Moreover, pesticide effects on Pacific oysters were also explored through experimental pathology assays...
Tipo: Text Palavras-chave: Pacific oyster; Pesticides; OsHV-1; Autophagy; Immunity; Hemocyte; Pathology.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00226/33740/32149.pdf
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Etude des interactions hôte/virus chez l’huître creuse Crassostrea gigas et son virus Ostreid herpesvirus 1 ArchiMer
Segarra, Amelie.
Ostreid herpesvirus type 1 (OsHV-1) can be considered one of the major infectious agents in Pacific oysters, Crassostrea gigas, in France and all around the world. Differences in terms of mortality among infected animals were reported suggesting a genetic basis of the susceptibility to the OsHV-1 infection. In this context, the main objective of this thesis was to understand the interactions between Pacific oysters and OsHV-1, in particular, the molecular basis of the viral cycle. Results showed that the virus is able to replicate in the host regardless of its stage of development or its susceptibility. However, multiplication kinetics is faster in the most susceptible individuals compared to less susceptible ones. After an active replication phase, it...
Tipo: Text Palavras-chave: Bivalves; Huître creuse; Crassostrea gigas; Mortalités; OsHV-1; Gènes viruax; Interaction hôte-virus; Transcriptome; Sensibilité; PCR en temps réel; Bivalve; Pacific oyster; Crassostrea ggias; Mortality; OsHV-1; Viral gene; Interaction host-virus; Transcriptome; Susceptibility; Real-time PCR.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00252/36297/34826.pdf
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Exploring First Interactions Between Ostreid Herpesvirus 1 (OsHV-1) and Its Host, Crassostrea gigas: Effects of Specific Antiviral Antibodies and Dextran Sulfate ArchiMer
Martenot, Claire; Faury, Nicole; Morga, Benjamin; Degremont, Lionel; Lamy, Jean-baptiste; Houssin, Maryline; Renault, Tristan.
Viral entry mechanisms of herpesviruses constitute a highly complex process which implicates several viral glycoproteins and different receptors on the host cell surfaces. This initial infection stage was currently undescribed for Ostreid herpes virus 1 (OsHV-1), a herpesvirus infecting bivalves including the Pacific oyster, Crassostrea gigas. To identify OsHV-1 glyproteins implicated in the attachment of the virus to oyster cells, three viral putative membrane proteins, encoded by ORF 25, 41, and 72, were selected and polyclonal antibodies against these targets were used to explore first interactions between the virus and host cells. In addition, effects of dextran sulfate, a negative charged sulfated polysaccharide, were investigated on OsHV-1 infection....
Tipo: Text Palavras-chave: OsHV-1; Antiviral antibodies; Dextran sulfate; Interaction; Crassostrea gigas; Infection.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00501/61273/64847.pdf
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Herpèsvirus infectant les bivalves marins : détection, génome et transmission ArchiMer
Arzul, Isabelle.
Since 1991, high mortality rates of larvae and juveniles of different bivalve mollusc species associated with herpesvirus infections have been reported in different countries. The aim of this study was to determine if more than one helpesvirus species was responsible for these infections or just one species exhibiting polymorphism. The investigations concentrated on the analysis of the virus genome, studies of virus transmission in different mollusc species and on the potential role of bivalve adults in the occurrence of infections. The analysis of various target sequences within the virus genome present in infected larvae samples demonstrated the presence of polymorphism. More notably a variant of the virus was identified and the variation appears...
Tipo: Text Palavras-chave: Herpèsvirus; Bivalves; Transmission; Polymorphisme; OsHV-1; Diagnostic; Herpesviruses; Bivalvia; Transmission; Polymorphism; OsHV-1; Diagnostic.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/00105/21675/19253.pdf
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In situ localization and tissue distribution of ostreid herpesvirus 1 proteins in infected Pacific oyster, Crassostrea gigas ArchiMer
Martenot, Claire; Segarra, Amelie; Baillon, Laury; Faury, Nicole; Houssin, Maryline; Renault, Tristan.
Immunohistochemistry (IHC) assays were conducted on paraffin sections from experimentally infected spat and unchallenged spat produced in hatchery to determine the tissue distribution of three viral proteins within the Pacific oyster, Crassostrea gigas. Polyclonal antibodies were produced from recombinant proteins corresponding to two putative membrane proteins and one putative apoptosis inhibitor encoded by ORF 25, 72, and 87, respectively. Results were then compared to those obtained by in situ hybridization performed on the same individuals, and showed a substantial agreement according to Landis and Koch numeric scale. Positive signals were mainly observed in connective tissue of gills, mantle, adductor muscle, heart, digestive gland, labial palps, and...
Tipo: Text Palavras-chave: OsHV-1; Viral proteins; Crassostrea gigas.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00326/43697/43124.pdf
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Influence of low temperatures on the survival of the Pacific oyster (Crassostrea gigas) infected with Ostreid herpesvirus type 1 ArchiMer
Pernet, Fabrice; Tamayo, David; Petton, Bruno.
Mortalities of oyster seed of Crassostrea gigas associated with ostreid herpes virus OsHV-1 μVar have been observed in many oyster producing countries since 2008. The objective of this study was to further investigate whether low temperature treatments can offer a viable option to mitigate oyster mortalities. An experiment was set-up to further evaluate the effect of low temperature treatments (10 and 13°C vs. 21°C) and their duration (6 d to 83 d) on the survival of oysters previously infected with OsHV-1 μVar by means of exposure to field conditions in areas where mortalities were occurring. Average survival of oysters infected with OsHV-1μVar was 71% after 83 d at low temperatures compared to only 23% in controls maintained at 21°C. During...
Tipo: Text Palavras-chave: Bivalve; Disease; OsHV-1; Oyster; Temperature; Virus.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00259/37047/35555.pdf
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Les pratiques culturales peuvent-elles permettre de réduire la surmortalité du naissain d'huîtres creuses? Récapitulatif des essais d'élevage et expérimentations zootechniques menés sur le territoire français entre 2008 et 2010 ArchiMer
Soletchnik, Patrick; Mazurie, Joseph; Allain, Gwenhael; Bedier, Edouard; Benabdelmouna, Abdellah; Blin, Jean-louis; Bouquet, Anne-lise; Cochet, Hélène; Degremont, Lionel; Gaussem, Florian; Gervasoni, Erika; Glize, Philippe; Petton, Bruno; Roussel, Pierre-yves; Pernet, Fabrice.
This paper summarizes knowledge about relations between husbandry practices and oyster spat mortalities since 2008 in France. These relations are strictly analyzed from a panel of experiments set on the shore (in outdoor experiments) by Ifremer teams and technical institutes all over French coasts. Results show some practices are able to reduce spat mortality with some influencing factors relevant of spat quality (sanitary status, natural or genetic selection) or husbandry practices. Spat “free” of virus is able to survive up to 90% when it is isolated and confined in ponds or nursery, during an epidemic crisis. When outbreaks occurred in oyster beds, natural or nursery spat died in almost same proportions. In 2010, some studies conducted in Mediterranean...
Tipo: Text Palavras-chave: Surmortalité; Naissain; Huître creuse; OsHV-1; Pratiques culturales; Mortality; Spat; Cupped oyster; Crassostrea gigas; OsHV-1; Cultural practices.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00032/14280/25847.pdf
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Les risques conchylicoles en Baie de Quiberon. Première partie : le risque de mortalité virale du naissain d’huître creuse Crassostra gigas. Rapport final du projet Risco 2010-2013 ArchiMer
Mazurie, Joseph; Stanisiere, Jean-yves; Langlade, Aime; Bouget, Jean-francois; Dumas, Franck; Treguier, Cathy; Leclerc, Emilie; Ravaud, Emilie; Quinsat, Kevin; Gabellec, Raoul; Retho, Michael; Cochet, Helene; Dreano, Alain.
This study (“Risco”), implicating both industry, socio-economic experts and biologists, was funded by the Regional Council of Brittany, for 3 years (2010-2013), to investigate upon the origin of oysters (Crassostrea gigas) mortalities in the bay of Quiberon (South Brittany, France). Specific mortalities of adult oysters were recorded in this bay in 2006, whereas mortalities of one year old spat were observed since in 2008 in this bay as at a national scale. The experimental protocol in 2010, including a monthly survey of oyster batches at 15 experimental stations, allowed to assess the risk for one-year oysters, due to viral disease. A clear spatial distribution of this risk was evidenced, with a western area spared from contamination and mortality, and a...
Tipo: Text Palavras-chave: Baie de Quiberon; Crassostrea gigas; Mortalité; Herpès-virus; OsHV-1; Épidémiologie; Modèle.; Quiberon bay; Crassostrea gigas; Mortality; Herpes-virus; OsHV-1; Epidemiology; Model.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00179/29043/27528.pdf
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Long-range PCR and high-throughput sequencing of Ostreid herpesvirus 1 indicate high genetic diversity and complex evolution process ArchiMer
Bai, Chang-ming; Morga, Benjamin; Rosani, Umberto; Shi, Jie; Li, Chen; Xin, Lu-sheng; Wang, Chong-ming.
Ostreid herpesvirus 1 (OsHV-1) is an important pathogen associated with mass mortalities of cultivated marine mollusks worldwide. Since no cell line allows OsHV-1 replication in vitro, it is difficult to isolate enough high-purity viral DNA for High-Throughput Sequencing (HTS). We developed an efficient approach for the enrichment of OsHV-1 DNA for HTS with long-range PCR. Twenty-three primer pairs were designed to cover 99.3% of the reference genome, and their performances were examined on ten OsHV-1 infected samples. Amplicon mixtures from six successfully amplified samples were sequenced with Illumina platform, and one of them (ZK0118) was also sequenced with the PacBio platform. PacBio reads were assembled into 2 scaffolds compared to 9−68 scaffolds...
Tipo: Text Palavras-chave: Long-range PCR; OsHV-1; High-throughput sequencing; Evolution; Diversity.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00464/57566/64184.pdf
Registros recuperados: 37
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