Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 12
Primeira ... 1 ... Última
Imagem não selecionada

Imprime registro no formato completo
A literature review as an aid to identify strategies for mitigating ostreid herpesvirus 1 in Crassostrea gigas hatchery and nursery systems ArchiMer
Rodgers, Chris; Arzul, Isabelle; Carrasco, Noèlia; Furones Nozal, Dolores.
An understanding of husbandry strategies and any associated risk factors is important for designing management control measures that can reduce mortality in Pacific oysters, Crassostrea gigas, caused by ostreid  herpesvirus 1 (OsHV-1). The type of culture facility can be considered in relation to the potential pathways that could lead to the entry of a pathogen and its survival. In addition, the animal host (e.g. age, physiological state,  selective breeding programmes), husbandry procedures (e.g. stocking density), the pathogen itself (e.g. pathogenicity, virulence) and environmental effects (e.g. temperature) represent other relevant interconnected factors. However, all these factors provide valuable background information for outlining the mitigation...
Tipo: Text Palavras-chave: Crassostrea gigas; Husbandry; Management strategies; Ostreid herpesvirus 1; Oyster herpesvirus; Risk factors.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00474/58581/61146.pdf
Imagem não selecionada

Imprime registro no formato completo
Detection and distribution of ostreid herpesvirus 1 in experimentally infected Pacific oyster spat ArchiMer
Segarra, Amelie; Baillon, Laury; Faury, Nicole; Tourbiez, Delphine; Renault, Tristan.
High mortality rates are reported in spat and larvae of Pacific oyster Crassostrea gigas and associated with ostreid herpesvirus 1 (OsHV-1) detection in France. Although the viral infection has been experimentally reproduced in oyster larvae and spat, little knowledge is currently available concerning the viral entry and its distribution in organs and tissues. This study compares OsHV-1 DNA and RNA detection and localization in experimentally infected oysters using two virus doses: a low dose that did not induce any mortality and a high dose inducing high mortality. Real time PCR demonstrated significant differences in terms of viral DNA amounts between the two virus doses. RNA transcripts were detected in oysters receiving the highest dose of viral...
Tipo: Text Palavras-chave: Crassostrea gigas; In situ hybridization; Ostreid herpesvirus 1; Viral DNA; Viral RNA.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00300/41099/40272.pdf
Imagem não selecionada

Imprime registro no formato completo
Dual transcriptomics of virus-host interactions: comparing two Pacific oyster families presenting contrasted susceptibility to ostreid herpesvirus 1 ArchiMer
Segarra, Amelie; Mauduit, Florian; Faury, Nicole; Trancart, Suzanne; Degremont, Lionel; Tourbiez, Delphine; Haffner, Philippe; Barbosa-solomieu, Valerie; Pepin, Jean-francois; Travers, Marie-agnes; Renault, Tristan.
Background: Massive mortality outbreaks affecting Pacific oyster (Crassostrea gigas) spat in various countries have been associated with the detection of a herpesvirus called ostreid herpesvirus type 1 (OsHV-1). However, few studies have been performed to understand and follow viral gene expression, as it has been done in vertebrate herpesviruses. In this work, experimental infection trials of C. gigas spat with OsHV-1 were conducted in order to test the susceptibility of several bi-parental oyster families to this virus and to analyze host-pathogen interactions using in vivo transcriptomic approaches. Results: The divergent response of these oyster families in terms of mortality confirmed that susceptibility to OsHV-1 infection has a significant...
Tipo: Text Palavras-chave: Interactions; Ostreid herpesvirus 1; Gene expression; Susceptibility; Crassostrea gigas.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00199/31029/29431.pdf
Imagem não selecionada

Imprime registro no formato completo
Experimental ostreid herpesvirus 1 infection of the Pacific oyster Crassostrea gigas: Kinetics of virus DNA detection by q-PCR in seawater and in oyster samples ArchiMer
Schikorski, David; Faury, Nicole; Pepin, Jean-francois; Saulnier, Denis; Tourbiez, Delphine; Renault, Tristan.
Herpes- and herpes-like viruses are known to infect a wide range of bivalve mollusc species throughout the world. Abnormal summer mortalities associated to the detection of ostreid herpesvirus 1 (OsHV-1) have been currently reported in France among larvae and spat of the Pacific cupped oyster Crassostrea gigas. In the present work, we have developed an experimental protocol of horizontal transmission based on the cohabitation between healthy and experimentally infected oysters. Through a cohabitation trial, the kinetics of OsHV-1 detection in different oyster organs and seawater samples were investigated and characterized for the first time using real time quantitative PCR.
Tipo: Text Palavras-chave: Ostreid herpesvirus 1; Cupped Pacific oyster; Crassostrea gigas; Experimental infection; Kinetic viral DNA quantification.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00025/13607/10688.pdf
Imagem não selecionada

Imprime registro no formato completo
First evaluation of resistance to both a California OsHV-1 variant and a French OsHV-1 microvariant in Pacific oysters ArchiMer
Divilov, Konstantin; Schoolfield, Blaine; Morga, Benjamin; Dégremont, Lionel; Burge, Colleen A.; Mancilla Cortez, Daniel; Friedman, Carolyn S.; Fleener, Gary B.; Dumbauld, Brett R.; Langdon, Chris.
Background Variants of the Ostreid herpesvirus 1 (OsHV-1) cause high losses of Pacific oysters globally, including in Tomales Bay, California, USA. A suite of new variants, the OsHV-1 microvariants (μvars), cause very high mortalities of Pacific oysters in major oyster-growing regions outside of the United States. There are currently no known Pacific oysters in the United States that are resistant to OsHV-1 as resistance has yet to be evaluated in these oysters. As part of an effort to begin genetic selection for resistance to OsHV-1, 71 families from the Molluscan Broodstock Program, a US West Coast Pacific oyster breeding program, were screened for survival after exposure to OsHV-1 in Tomales Bay. They were also tested in a quarantine laboratory in...
Tipo: Text Palavras-chave: Ostreid herpesvirus 1; Crassostrea gigas; Heritability; Breeding.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00598/70974/69206.pdf
Imagem não selecionada

Imprime registro no formato completo
Influence of OSHV-1 oyster mortality episode on dissolved inorganic fluxes: An ex situ experiment at the individual scale ArchiMer
Richard, Marion; Bourreau, Juliette; Montagnani, Caroline; Ouisse, Vincent; Le Gall, Patrik; Fortune, Martine; Munaron, Dominique; Messiaen, Gregory; Callier, Myriam; Roque D'Orbcastel, Emmanuelle.
Ostreid herpesvirus 1 (OsHV-1 μvar) infection has caused significant mortalities in juvenile oysters (Crassostrea gigas). In contrast to the practices of other animal production industries, sick and dead oysters are not separated from live ones and are left to decay in the surrounding environment, with unknown consequences on fluxes of dissolved materials. A laboratory approach was used in this study to test the influence of oyster mortality episode on dissolved inorganic fluxes at the oyster interface, dissociating (i) the effect of viral infection on metabolism of juvenile oysters and (ii) the effect of flesh decomposition on oxygen consumption and nutrient releases at the individual scale. Nine batches of juvenile oysters (Individual Total wet weight...
Tipo: Text Palavras-chave: Crassostrea gigas; Mortality; Ostreid herpesvirus 1; Juvenile; Spat; Mineralisation; Oxygen consumption; Nutrient fluxes.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00378/48973/49380.pdf
Imagem não selecionada

Imprime registro no formato completo
Insights on the association between somatic aneuploidy and ostreid herpesvirus 1 detection in the oysters Crassostrea gigas, C. angulata and their F1 hybrids ArchiMer
Batista, Frederico M.; Lopez-sanmartin, Monserrat; Boudry, Pierre; Navas, Jose I.; Ruano, Francisco; Renault, Tristan; Fonseca, Vera G.; Leitao, Alexandra.
Cytogenetic abnormalities associated with viral infections, including from viruses of the Herpesvirales order, have been reported in vertebrate species. Ostreid herpesvirus 1 (OsHV-1) has been detected worldwide during mortality outbreaks of the Pacific oyster Crassostrea gigas. On the other hand, a high proportion of aneuploid cells in somatic tissues have been observed in C. gigas. In this study, we analysed the putative association between aneuploidy levels and the detection of OsHV-1 in gills of C. gigas, the Portuguese oyster C. angulata and their F1 hybrids cultured in Ria Formosa (Portugal). OsHV-1 was detected by PCR in 5.4% of the total of oysters analysed (n = 111) namely in 11.1%, 8.0% and 1.7% of C. gigas, C. angulata and F1 hybrid...
Tipo: Text Palavras-chave: Aneuploidy; Ostreid herpesvirus 1; Oyster; Crassostrea.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00215/32581/35759.pdf
Imagem não selecionada

Imprime registro no formato completo
Les virus infectant les mollusques marins : un exemple d'actualité, les herpèsvirus ArchiMer
Renault, Tristan.
Although viruses have been associated with mortality outbreaks in different mollusc species, little information is available on their exact affiliation and taxonomic position. Herpes viruses have attracted particular attention because of their economic and ecological impact on both farmed and wild marine molluscs over the past 20 years. Investigations based on molecular approaches have been carried out on the herpes virus infecting Pacific oysters in France. Molecular and ultra-structural data were used to classify the virus under the name ostreid herpesvirus 1 (OsHV-1), as the first known species in the family Malacoherpesviridae. Since 2008, massive mortality outbreaks were reported in several Crassostrea gigas oyster farms in France, Ireland, and the...
Tipo: Text Palavras-chave: Molluscs; Pacific cupped oyster; Herpes virus; Ostreid herpesvirus 1; Excess mortality.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00069/18000/15562.pdf
Imagem não selecionada

Imprime registro no formato completo
Long dsRNAs promote an anti-viral response in Pacific oyster hampering ostreid herpesvirus 1 replication ArchiMer
Pauletto, Marianna; Segarra, Amelie; Montagnani, Caroline; Quillien, Virgile; Faury, Nicole; Le Grand, Jacqueline; Miner, Philippe; Petton, Bruno; Labreuche, Yannick; Fleury, Elodie; Fabioux, Caroline; Bargelloni, Luca; Renault, Tristan; Huvet, Arnaud.
Double-stranded RNA (dsRNA)-mediated genetic interference (RNAi) is a widely used reverse genetic tool for determining the loss-of-function phenotype of a gene. Here, the possible induction of an immune response by long dsRNA was tested in a marine bivalve (Crassostrea gigas), as well as the specific role of the subunit 2 of the nuclear factor kappa B inhibitor (I kappa B2). This gene is a candidate of particular interest for functional investigations in the context of oyster mass mortality events, as Cg-I kappa B2 mRNA levels exhibited significant variation depending on the amount of ostreid herpesvirus 1 (OsHV-1) DNA detected. In the present study, dsRNAs targeting Cg-I kappa B2 and green fluorescent protein genes were injected in vivo into oysters...
Tipo: Text Palavras-chave: Anti-viral response; RNA interference; Inhibitor of NF-kappa B; Marine bivalve; Ostreid herpesvirus 1.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00409/52011/52698.pdf
Imagem não selecionada

Imprime registro no formato completo
Ostreid herpesvirus 1 infection in farmed Pacific oyster larvae Crassostrea gigas (Thunberg) in Korea ArchiMer
Hwang, J. Y.; Park, J. J.; Yu, H. J.; Hur, Y. B.; Arzul, Isabelle; Couraleau, Yann; Park, M. A..
Tipo: Text Palavras-chave: Crassostrea gigas; Korea; Ostreid herpesvirus 1; Pacific oyster.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00162/27276/26231.pdf
Imagem não selecionada

Imprime registro no formato completo
Rapid and sensitive detection of ostreid herpesvirus 1 in oyster samples by real-time PCR ArchiMer
Pepin, Jean-francois; Riou, Antoine; Renault, Tristan.
Herpes and herpes-like virus infections have been reported in various marine mollusc species associated with high mortality rates. Following the characterisation and genome sequencing of ostreid herpesvirus 1 (OsHV-1), specific diagnostic tools have been developed based on conventional PCR techniques or in situ hybridisation. We have now developed a real-time PCR assay for rapid, sensitive and quantitative detection of OsHV-1, and compared it with a conventional PCR technique described previously. The new assay utilised SYBR® Green chemistry with specific primers C9/C10 targeting the C region. The melt curve analysis of OsHV-1 DNA or DNA extracted from infected material showed only one melting temperature peak (75.75 ± 0.1 °C). The assay had a detection...
Tipo: Text Palavras-chave: SYBR® Green; Real time PCR; Crassostrea gigas; Ostreid herpesvirus 1; Herpesvirus.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3951.pdf
Imagem não selecionada

Imprime registro no formato completo
Temperature influence on pathogen transmission and subsequent mortalities in juvenile Pacific oysters Crassostrea gigas ArchiMer
Petton, Bruno; Pernet, Fabrice; Robert, Rene; Boudry, Pierre.
Since 2008, mass mortalities of 1-yr-old Crassostrea gigas associated with the ostreid herpesvirus OsHV-1 μVar have occurred along all the coasts of France when seawater temperature reaches 16 to 17°C. The present study aimed to characterize the effect of temperature on oyster survival in combination with OsHV-1 DNA quantification by standard real-time PCR and total vibrio population levels in oyster tissues. To examine the effect of seawater temperature on disease transmission and related mortality of oysters, cohabitation experiments were conducted between healthy naïve oysters and oysters previously exposed to field conditions in areas where mortalities were occurring. Oysters initially maintained in controlled conditions (free of mortality and negative...
Tipo: Text Palavras-chave: Juvenile oyster; Pathology; Temperature; Survival; Vibrios; Ostreid herpesvirus 1.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00148/25891/23912.pdf
Registros recuperados: 12
Primeira ... 1 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional