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Registros recuperados: 19
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A segment and epithelium specific messenger ribonucleic acid fragment up-regulated by estradiol in the rat oviduct Biol. Res.
RIOS,MARIANA; OJEDA,SUANY; VELASQUEZ,LUIS A; MAISEY,KEVIN; CROXATTO,HORACIO B.
Estradiol accelerates oviductal embryo transport in the rat through changes of genomic expression in oviductal cells. However, the genes involved are unknown. We used a differential display by reverse transcription-polymerase chain reaction to detect estradiol (E2)-dependent genes in the rat oviduct. Rats on day 2 of pregnancy were untreated or treated with 10 µg of E2 and the oviducts were extracted at 30, 180 and 360 min later and used to isolate RNA. Products of reverse transcriptase-PCR, made with pairs of arbitrary and oligo-deoxythymidine primers, were separated on denaturing polyacrylamide gels and candidate bands were excised and reamplified. Truly positive cDNA fragments determined by a single strand conformation polymorphism assay were cloned and...
Tipo: Journal article Palavras-chave: Differential display; Estradiol; Gene; MRNA; Oviduct.
Ano: 2001 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100011
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Accumulation of Eosinophils in the Infundibulum of the Bovine Oviduct Just after Ovulation OAK
KOWSAR, Rasoul; JIEMTAWEEBOON, Sineenard; SHIRASUNA, Koumei; SHIMIZU, Takashi; SASAKI, Motoki; KITAMURA, Nobuo; MIYAMOTO, Akio; 清水, 隆; 佐々木, 基樹; 宮本, 明夫.
This study was to investigate the localization and distribution of eosinophils (EOS) in the bovine oviduct throughout the estrous cycle. Histological studies revealed more abundant EOS in the infundibula of the oviducts ipsilateral to the preovulatory dominant follicle and the ovulated ovary. The number of EOS was higher in the infundibula of the oviducts ipsilateral to the ovulated ovary than those of the oviducts contralateral to the ovulated ovary. The infundibula of the oviducts ipsilateral to the preovulatory dominant follicle had higher number of EOS than those of the oviducts ipsilateral to the mid-cycle corpus luteum. The number of EOS in the isthmus, but not in the ampulla, was higher in the outer layers (tunica muscularis and tunica serosa) than...
Palavras-chave: Bovine; Eosinophil; Oviduct; Ovulation.
Ano: 2014 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3947
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An Acute-phase Protein as a Regulator of Sperm Survival in the Bovine Oviduct: Alpha 1-acid-glycoprotein Impairs Neutrophil Phagocytosis of Sperm In Vitro OAK
LIU, Jinghui; A. MAREY, Mohamed; KOWSAR, Rasoul; HAMBRUCH, Nina; SHIMIZU, Takashi; HANEDA, Shingo; MATSUI, Motozumi; SASAKI, Motoki; HAYAKAWA, Hiroyuki; PFARRER, Christiane; MIYAMOTO, Akio; 羽田, 真悟; 松井, 基純; 佐々木, 基樹; 宮本, 明夫.
We have previously shown that polymorphonuclear neutrophils (PMNs) are present in bovine oviduct fluid under physiological conditions, and that the oviduct provides a microenvironment that protects sperm from phagocytosis by PMNs. Alpha 1-acid glycoprotein (AGP) is a major acute-phase protein produced mainly in the liver that has immunomodulatory functions. AGP mRNA is expressed in extrahepatic organs, such as the lung, kidney, spleen, lymph node, uterus, and ovary. Therefore, in this study, we investigated, 1) the local production of AGP in the bovine oviduct, 2) the effect of AGP on the phagocytic activity of PMNs for sperm and superoxide production and 3) the impact of AGP desialylation on the PMN phagocytosis of sperm. The AGP gene was expressed in...
Palavras-chave: Alpha 1-acid glycoprotein (AGP); Bovine; Oviduct; Phagocytosis; Sperm.
Ano: 2014 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3946
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Effects of Laser Beak Trimming on the Development of Brown Layer Pullets Rev. Bras. Ciênc. Avic.
Petrolli,TG; Petrolli,OJ; Girardini,LK; Zotti,MLAN; Baggio,RA; Junqueira,OM.
ABSTRACT Laser beak trimming is a promising alternative to conventional hot-blade beak trimming as it is less painful and provides better beak uniformity and better animal welfare. In this study, laser vs. hot-blade beak trimming were compared in 400 Hy-line Brown pullets between 2-16 weeks of age. At 7 days of age, birds were distributed according to a completely randomized design into two treatments, with 10 replicates (pens) of 20 birds each. Birds and feed offer and residues were weekly weighed to determine weight gain, feed intake, and feed conversion ratio. In weeks 4, 8, 12, and 16 weeks, one bird per experimental unit was sacrificed to measure the development of the heart development, liver, proventriculus, gizzard, small intestine, and bursa. And...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Animal welfare; Layers; Organs; Oviduct.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000100123
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Endothelin-1 downregulates sperm phagocytosis by neutrophils in vitro: a physiological implication in bovine oviduct immunity OAK
MAREY, Mohamed Ali; YOUSEF, Mohamed Samy; LIU, Jinghui; MORITA, Kazuhiro; SASAKI, Motoki; HAYAKAWA, Hiroyuki; SHIMIZU, Takashi; ELSHAHAWY, Ibrahim I.; MIYAMOTO, Akio; 佐々木, 基樹; 清水, 隆; 宮本, 明夫.
The oviduct is an active contractile tube that provides the proper environment for sperm transport, capacitation and survival. Oviductal contractions are regulated by autocrine/paracrine secretion of several factors, such as prostaglandins (PGs) and endothelin-1 (EDN-1). We have previously shown that during the preovulatory stage, sperm are exposed to polymorphonuclear neutrophils (PMNs) in the bovine oviduct, and the bovine oviduct epithelial cells (BOECs) secrete molecules including PGE2 that suppress sperm phagocytosis by PMNs in vitro. In this study, we investigated the possible effects of EDN-1 on the phagocytic activity of PMNs toward sperm. The local concentrations of EDN-1 in oviduct fluid and BOEC culture medium ranged from 10–10 to 10–11 M as...
Palavras-chave: Endothelin-1; Oviduct; Phagocytosis; Polymorphonuclear neutrophils (PMN); Sperm.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4310
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Evaluation of induced molting methods on the livability and reproductive system regression of Japanese quails (Coturnix japonica) Rev. Bras. Ciênc. Avic.
Teixeira,RSC; Cardoso,WM; Nogueira,GC; Câmara,SR; Romao,JM; Siqueira,AA; Sampaio,FAC; Moraes,TGV; Campello,CC; Buxade,CC.
This study aimed at evaluating the alternative method of zinc oxide and fasting to induce molt in Japanese quails. A total number of 190 48-week-old quails was used. They were at end of laying cycle, and presented low egg production. Quails molted by zinc oxide (Z) were fed a diet containing 25,000 ppm of zinc oxide, and received water ad libitum. Quails treated by fasting (F) received no feed and a day of water restriction. The treatment period was determined by the experimental level of body weight loss (BWL). Birds were submitted to different levels of BWL in order to analyze reproductive system regression (ovary + oviduct), and livability. The following groups were established according to their BWL: Control (untreated quails); F25 (25% BWL by F); F35...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Forced molting; Ovary; Oviduct; Quail; Zinc oxide.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2007000200002
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Expression of Nerve Growth Factor (NGF) and Its Receptors TrkA and p75 in the Reproductive Organs of Laying Hens Rev. Bras. Ciênc. Avic.
Shaoxia,PU; Changwei,QU; Zhi,LI; Yansen,LI; ChunMei,LI.
Abstract In order to investigate the expression levels of nerve growth factor (NGF) and its receptors (TrkA and p75) in prehierarchical follicles and oviducts of hens, five 130-day-old laying hens were examined by immunohistochemistry and RT-PCR analysis. NGF and its receptors were expressed in theca cells and granulosa cells of prehierarchical follicles, and they were also expressed in the epithelial cells of oviducts. The expression of the genes NGF, TrkA and p75 were significantly different in prehierarchical follicles (p<0.05 or p<0.01), and NGF and TrkA gene expression was significantly different in different parts of oviduct (p<0.05 or p<0.01). The expression of NGF and p75 mRNA levels was highest in large white follicle (LWF), as well as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: NGF; TrkA; P75; Prehierarchical follicle; Oviduct; Laying hen.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000100187
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Genital Sensory Stimulation Shifts Estradiol Intraoviductal Signaling from Nongenomic to Genomic Pathways, Independently from Prolactin Surges Biol. Res.
PEÑARROJA-MATUTAN0,C; PARADA-BUSTAMANTE,A; ORIHUELA,P A; RÍOS,M; VELASQUEZ,LUIS A; CROXATTO,H B.
Estradiol (E2) accelerates oviductal egg transport through nongenomic pathways involving oviductal protein phosphorylation in non-mated rats, and through genomic pathways in mated rats. Here we investigated the ability of cervico-vaginal stimulation (CVS) to switch the mode of action of E2 in the absence of other male-associated components. Pro-estrous rats were subjected to CVS with a glass rod and 12 hours later were injected subcutaneously with E2 and intrabursally with the RNA synthesis inhibitor Actinomycin D or the protein phosphorylation inhibitor H-89. The number of eggs in the oviduct, assessed 24 h later, showed that Actinomycin D, but not H-89 blocked the E2-induced egg transport acceleration. This clearly indicates that CVS alone, without other...
Tipo: Journal article Palavras-chave: Mating; Oviduct; Estradiol; Signaling pathways; Prolactin receptor; Egg transport; Rat.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200012
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Histologia e histoquímica do magno, um dos segmentos do oviduto de Numida meleagris (Linné) (Numididae, Galliformes) Zoologia
Ribeiro,Maria das Graças; Teles,Maria Eloiza de Oliveira; Maruch,Sandra Maria das Graças.
Numida meleagris (Linné, 1758) is an african bird that was brought to Brazil. As the bird adapted to the climate of this new habitat and spread ali around the country, it is nowadays part of our avifauna. The present study continues topographic and morphofunctional researches on the female genital apparatus of this species, since it describes histological and histochemical aspects of magnum, a segment of oviduct. Magnum was dissected and processed according to routine and histochemical staining procedures to detect glycogen and mucous substances in the epithelial tissue and mucous glands. Besides focusing morphological aspects, the study compares the data obtained with those of other species aiming to contribute to the enlargement of the knowledge on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Galliformes; Numididae; Female genital; Oviduct; Magnum.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751997000100020
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HISTOLOGICAL DESCRIPTION OF THE REPRODUCTIVE SYSTEM OF MALE AND FEMALE HATCHLINGS OF THE MAGDALENA RIVER TURTLE (Podocnemis lewyana) Acta biol.Colomb.
SÁNCHEZ-OSPINA,ANDRÉS C; RODRIGUEZ,BERARDO; CEBALLOS,CLAUDIA P..
In this study we described the macroscopic and microscopic histology of the reproductive system of male and female Podocnemis lewyana neonates (3.5 months old). We found macroscopic differences in the morphology of the gonads between the sexes, with ovaries being twice as long as testes, and testes being twice as wide as ovaries. Microscopically, we identified several immature elements, such as the lack of a muscle layer in the oviduct of females, and simple epithelia instead of pseudostratified epithelia in the oviduct and epididymis described in reptile adults. We also found a black pigment observed macroscopically in the mesovarium, and macroscopically and histologically in the epididymis. This pigment is consistent with the center of melano-macrophages...
Tipo: Journal article Palavras-chave: Epididymis; Histology; Ovary; Oviduct; Pigment; Testis.
Ano: 2014 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2014000300009
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In vivo expression of ß-galactosidase by rat oviduct exposed to naked DNA or messenger RNA Biol. Res.
RIOS,MARIANA; VENEGAS,ALEJANDRO; CROXATTO,HORACIO B.
Intra-oviductal administration of RNA obtained from oviducts of estradiol-treated rats resulted in accelerated egg transport (<A HREF="#rios97">Ríos et al., 1997</A>). It is probable that estradiol-induced messenger RNA (mRNA) entered oviductal cells and was translated into the proteins involved in accelerated egg transport. In order to test this interpretation we deposited in vivo 50 µg of pure ß-galactosidase (ß-gal) mRNA, 50 µg of pure DNA from the reporter gene ß-gal under SV40 promoter or the vehicle (control oviducts) into the oviductal lumen of rats. Twenty four hours later the ß-gal activity was assayed in oviductal tissue homogenates using o-nitrophenyl-ß-D-galactopyranoside as a substrate. The administration of ß-gal mRNA and pSVBgal...
Tipo: Journal article Palavras-chave: SS-galactosidase; Gene transfer; Oviduct.
Ano: 2002 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300007
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Morphogenesis of a Tunica Mucosa of Oviduct of the Hens International Journal of Morphology
Khokhlov,R. YU.; Kuznetcov,S. I..
The authors have used non-laying and laying hens (Lohmann Brown), in order to study morphological aspects development of tunica mucosa of the oviduct. The results showed that the oviduct of the hen is well-developed at the left side, atrophied at the right side and consists of five different regions: infundibulum, magnum, isthmus, shell-gland and excretory section (vagina). During 1-120 days in oviduct is the most advanced caudal part, where height and width folds of a tunica mucosa is more than in cranial part, that probably, is caused with the future function of these departments. In the period oviposition (150-540 days), the tunica mucosa is most advanced in such departments, as: magnum, isthmus, shell-gland
Tipo: Journal article Palavras-chave: Oviduct; Anatomy; Histology; Morphology.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000200014
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Morphology and Histological Annual Changes of the Oviduct of Chaetophractus villosus (Mammalia, Xenarthra, Dasypodidae) International Journal of Morphology
Codón,Stella Maris; Casanave,Emma Beatriz.
The morphological features of the oviduct in the armadillo Chaetophractus villosus (Desmarest, 1804) were studied. In the simple columnar epithelium, ciliated cells, secretory cells, and cells of the bottom of the folds are described. The most remarkable findings are the increase in number of ciliated cells, when present, from the fimbriae to the uterus, being the isthmus practically lined by ciliated cells, and that secretory cells are more abundant in the apical zone of the ampulla and fimbriae, than in the isthmus. Morphological changes along the year in the different portions are described.
Tipo: Journal article Palavras-chave: Xenarthra; Armadillo; Chaetophractus villosus; Morphology; Oviduct.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000200009
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Morphology of an Infundibulum of the Oviduct of the Sexually Mature Hens International Journal of Morphology
Khokhlov,R.Y..
Studied functional morphology of an infundibulum oviduct of the hens in an age interval 150-540 days. As indicators of development the following parameters served: length, weight, diameter of a infundibulum, sizes epithelial cells, height and width folds of a túnica mucosa, histological structure of a wall of a infundibulum. The results show dynamical increase of investigated parameters with 150- till 360-day's age, with their subsequent decrease to 540-day's age.
Tipo: Journal article Palavras-chave: Oviduct; Reproductive system; Morphology; Layer.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000400016
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Morphology of the urogenital papilla and its component ducts in Astyanax altiparanae Garutti &amp; Britski, 2000 (Characiformes: Characidae) Neotropical Ichthyology
Siqueira-Silva,Diógenes Henrique de; Silva,Amanda Pereira dos Santos; Ninhaus-Silveira,Alexandre; Veríssimo-Silveira,Rosicleire.
The histological description of the urogenital papilla is an important tool to comprehension of the reproductive mechanisms in fish, as well as a pre-requisite to germ cell transplantation in adult fish, besides to be a good biological indicator to environmental changes. Was performed the histological description of the urogenital papilla and its component ducts in the tetra Astyanax altiparanae. The genital and urinay ducts pass separately throughout most part of its extension, joining in a single duct before opening. In males this opening is asymmetric and seems to have double origin, being completely surrounded by striated muscle fibers, while in females it is symmetric and the muscle fibers does not surround it totally. Spermatic duct and oviduct...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish reproduction; Histological analysis; Oviduct; Sperm duct; Urinary duct.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000200309
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N-acetylglucosaminyltransferase I Activity of Bovine Oviduct Epithelial Cells: Stimulation by Luteinizing Hormone, Vascular Endothelial Growth Factor and Tumor Necrosis Factor α OAK
NOZAKI, Hirofumi; WIJAYAGUNAWARDANE, Missaka P.B.; KODITUWAKKU, Suranga P.; YOSHIDA, Takashi; NAKAMURA, Tadashi; ARAI, Ikichi; URASHIMA, Tadasu; MIYAMOTO, Akio; 中村, 正; 浦島, 匡; 宮本, 明夫.
N-acetylglucosaminyltransferase I (GnT I; EC 2.4.1.101), which catalyzes the first step in the conversion of oligomannose to complex or hybrid N-glycans of glycoproteins, was found in media cultured with bovine oviduct epithelial cells (BOEC) obtained from non-pregnant cows during the follicular phase. Combined treatment with specific hormones increased GnT I release from BOEC. Luteinizing hormone (LH; 10 ng/ml) alone slightly, but together with 17β-estradiol (E2; 1 ng/ml), synergistically increased GnT I activity. Vascular endothelial growth factor (VEGF) and tumor necrosis factor (TNF) α, which have been shown to have their highest activities in the bovine oviduct during the periovulatory period, also increased in GnT I activity. This study provides the...
Palavras-chave: Bovine; Luteinizing hormone (LH); N-acetylglucosaminyltransferase I; Oviduct; Tumor necrosis factor (TNF) α; Vascular endothelial growth factor (VEGF).
Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2978
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RNA fingerprinting using RAP-PCR identifies an EBAF homologue mRNA differentially expressed in rat oviduct Biocell
Valdecantos,Pablo A.; Argañaraz,Martín E.; Abate,Carlos M.; Miceli,Dora C..
As a step towards the identification of genes preferentially expressed in the oviduct during early rat embryo development, we isolated a cDNA fragment (Pr14) by using RNA arbitrarily primed PCR (RAP-PCR), being its expression restricted to oviduct and uterus; its mRNA is mainly expressed in oviduct during late luteal phase and early pregnancy. This fragment is 100% identical to a rat DNA sequence (Accession No. NW_047400) downstream the terminal exon of a Rattus norvegicus gene (Locus Link Accession No. LOC289316) similar to ebaf (endometrial bleeding-associated factor), a novel member of the Transforming Growth Factor superfamily. Northern analyses showed that this sequence hybridizes with 2.9 kb and 4.1 kb mRNAs in early pregnant rat oviducts. However,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Differential gene expression; Oviduct; RNA fingerprint; CDNA.
Ano: 2004 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000300005
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Segment­Specific Expression of MMP/TIMP in the Oviduct of Llama (Lama glama) and Gelatinolytic Activity in the Oviductal Fluid International Journal of Morphology
Zampini,Renato; Apichela,Silvana; Sari,Luciana; Angiono,Georgina; Lombardo,Daniel; Miceli,Dora; Argañaraz,Martin.
Oviductal molecules have the potential to improve the reproductive biotechnologies. In camelids, knowledge and assessment of the oviductal environment are necessary to successfully develop species-specific reproductive technologies, especially because of the camelids reproductive particularities. Among the oviductal factors, the matrix metalloproteinases/tissue inhibitor of matrix metalloproteinases system (MMPs/TIMPs) should be investigated more thoroughly due to their participation in reproductive processes. Consequently, the current study assayed gene and protein expression of MMPs throughout the llama oviduct. MMPs zymogen and active forms in the oviductal fluid were also characterized. MMP2 and MMP9 transcripts were detected in ampulla, isthmus,...
Tipo: Journal article Palavras-chave: Oviduct; Llama; MMPs; TIMPs; Gene expression levels.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200038
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The Differences Among Lines on Oviduct and Cumulative Follicle Weight in Laying Hens OAK
三好, 俊三; 高橋, 洋明; ルック, キィウ ミン; 口田, 圭吾; 光本, 孝次.
Palavras-chave: 卵管; 膨大部; 卵巣; 黄色卵胞; 採卵鶏; Oviduct; Magnum; Ovary; Follicle; Laying hen.
Ano: 1996 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/444
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