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A factorial design applied to the study of chromium toxicity on the glutathione levels of Brachiaria brizantha and Brachiaria ruziziensis seedlings. Repositório Alice
MARQUES, R.; OLIVEIRA, M. A. L. de; REIS, C. R. G. dos; KOPP, M. M.; PASSOS, L. P..
2015
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Brachiaria ruziziensis; Brachiaria brizantha; Glutathione; Chromium-toxicity; Oxidative stress.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1035418
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Active and passive biomonitoring suggest metabolic adaptation in blue mussels (Mytilus spp.) chronically exposed to a moderate contamination in Brest harbor (France) ArchiMer
Lacroix, Camille; Richard, Gaelle; Seguineau, Catherine; Guyomarch, Julien; Moraga, Dario; Auffret, Michel.
Brest harbor (Bay of Brest, Brittany, France) has a severe past of anthropogenic chemical contamination, but inputs tended to decrease, indicating a reassessment of its ecotoxicological status should be carried out. Here, native and caged mussels (Mytilus spp.) were used in combination to evaluate biological effects of chronic chemical contamination in Brest harbor. Polycyclic aromatic hydrocarbon (PAH) contamination was measured in mussel tissues as a proxy of harbor and urban pollution. Biochemical biomarkers of xenobiotic biotransformation, antioxidant defenses, generation of reducing equivalents, energy metabolism and oxidative damage were studied in both gills and digestive glands of native and caged mussels. In particular, activities of...
Tipo: Text Palavras-chave: Marine biomonitoring; Chronic pollution; Blue mussel Mytilus spp.; Caging; Oxidative stress; Energetic metabolism; Bay of Brest.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00255/36654/35795.pdf
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Acute toxicity of commercial atrazine in Piaractus mesopotamicus: histopathological, ultrastructural, molecular, and genotoxic evaluation. Repositório Alice
PAIVA, P. P. de; DELCORSO, M. C.; MATHEUS, V. A.; QUEIROZ, S. C. do N. de; COLLARES-BUZATO, C. B.; ARANA, S..
The aim of this work was to evaluate the sensitivity of Pacu fingerlings (Piaractus mesopotamicus) by measuring the effects of median lethal concentration (LC50) of atrazine (ATZ - 28.58 mg/L) after acute exposure (up to 96 h). The fish were exposed to the LC50 of ATZ for 96 h (28.58 mg/L) in a static system. During the experiment, the fingerlings were randomly distributed in four glass tanks (50 L) containing dechlorinated water. Four glass tanks were for the control group, and four were for the ATZ-exposed group (n=4 per glass tank), given a total number of 16 animals tested per group. The genotoxicity was evaluated by micronucleus (MN) test in erythrocytes from peripheral blood. Qualitative and semi-quantitative histopathological analyses, and also...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Brazilian ichthyofauna; Kidney lesions; Liver lesions; Micronucleus test; Pacu; Herbicida; Toxidez; Poluição da água; Piaractus mesopotamicus; Water pollution; Atrazine; Oxidative stress; Nephrotoxicity; Hepatotoxicity.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080546
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Analysis of Myenteric Neurons of the Cecum of Diabetic Rats After Supplementation with Ascorbic Acid International Journal of Morphology
Zanoni,Jacqueline Nelisis; Pereira,Priscila Goncalves Cardoso; dos Santos Pereira,Marli Aparecida.
The objective of this work was to investigate the neuroprotective action of the ascorbic acid over the myenteric neurons in the cecum of Wistar rats, four months after induction of the diabetes mellitus experimental with streptozotocin. Three groups with five rats each were used: C- controls, D- diabetic, DA- diabetic treated with ascorbic acid. For evidentiation of the myenteric neurons was carried out to Giemsa's technique. Were evaluated the areas of cell bodies of 500 neurons in each group studied. The quantitative analysis was carried out in an area of 16.6 mm2 in each cecum studied. In the animals diabetic observed elevation of the glycemia and glycated hemoglobin. The supplementation with ascorbic acid was effective under the myenteric neurons of...
Tipo: Journal article Palavras-chave: Ascorbic acid; Diabetes mellitus; Oxidative stress; Cecum; Streptozotocin.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000200013
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Antioxidant and anti hyperglycemic role of wine grape powder in rats fed with a high fructose diet Biol. Res.
Hernández-Salinas,Romina; Decap,Valerie; Leguina,Alberto; Cáceres,Patricio; Perez,Druso; Urquiaga,Ines; Iturriaga,Rodrigo; Velarde,Victoria.
BACKGROUND: Metabolic syndrome is a growing worldwide health problem. We evaluated the effects of wine grape powder (WGP), rich in antioxidants and fiber, in a rat model of metabolic syndrome induced by a high fructose diet. We tested whether WGP supplementation may prevent glucose intolerance and decrease oxidative stress in rats fed with a high fructose diet. METHODS: Male Sprague-Dawley rats weighing 180 g were divided into four groups according to their feeding protocols. Rats were fed with control diet (C), control plus 20 % WGP (C + WGP), 50 % high fructose (HF) or 50 % fructose plus 20 % WGP (HF + WGP) for 16 weeks. Blood glucose, insulin and triglycerides, weight, and arterial blood pressure were measured. Homeostasis model assessment (HOMA) index...
Tipo: Journal article Palavras-chave: Insulin resistance; Oxidative stress; Metabolic syndrome; Wine grape powder.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100053
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Antioxidant defenses of irrigated forage sorghum with saline aquaculture effluent. Repositório Alice
GUIMARÃES, M. J. M.; SIMOES, W. L.; CAMARA, T. de J. R.; SILVA, C. U. de C; WILLADINO, L. G..
The objective of this work was to evaluate the biomass production and antioxidant enzymatic system activity of irrigated forage sorghum with saline aquaculture effluent under different leaching fractions. The experiment was conducted in the Caatinga Experimental Field of the Embrapa Semiarido, in Petrolina, State of Pernambuco, Brazil. The experimental design was a complete randomized block in a split-plot arrangement with four replications, consisting of three forage sorghum varieties (Volumax, F305 and Sudan) and four leaching fractions (0, 5, 10 and 15%). The vegetal materials were collected when the plants were at the soft-dough stage. The biomass production and activity of the enzymes superoxide dismutase, catalase and ascorbate peroxidase were...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Água salina; Sorghum bicolor; Enzimas; Estresse oxidativo; Sorgo forrageiro; Irrigação; Enzymes; Oxidative stress; Sorghum.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1089388
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Behavior of coffee seeds to desiccation tolerance and storage. Repositório Alice
ABREU, L. A. de S.; VEIGA, A. D.; VON PINHO, E. V. de R.; MONTEIRO, F. F.; ROSA, S. D. V. F. da.
The technology developed by breeding programs is applied to coffee seeds; however, after processing and drying, they lose viability within a short period of time, thus making storage unsuitable. The objective of this research was to evaluate the quality of coffee seeds submitted to different drying methods and moisture contents during storage. The coffee seeds were submitted to conventional drying (slow shade drying) and fast drying in a static drier until they reached a moisture content of 40, 20, 1 2 and 5%. After this process, the seeds were stored in a cold chamber for 12 months, and seed quality was evaluated before and during storage by the germination test, electrophoretic patterns of heat resistant proteins, and the activity of isoenzyme systems....
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Desiccation tolerance; Estresse oxidativo; Tolerância à dessecação; Coffea arabica; Desitratação; Longevidade; Longevity; Coffea arabica; Oxidative stress.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1039780
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Behavioural and biochemical responses of two marine invertebrates Scrobicularia plana and Hediste diversicolor to copper oxide nanoparticles ArchiMer
Buffet, Pierre-emmanuel; Tankoua, Olivia Fossi; Pan, Jin-fen; Berhanu, Deborah; Herrenknecht, Christine; Poirier, Laurence; Amiard-triquet, Claude; Amiard, Jean-claude; Berard, Jean-baptiste; Risso, Christine; Guibbolini, Marielle; Romeo, Michele; Reip, Paul; Valsami-jones, Eugenia; Mouneyrac, Catherine.
Engineered nano-sized Cu oxide particles are extensively used in diverse applications. Because aquatic environments are the ultimate "sink" for all contaminants, it is expected that nanoparticles (NP) will follow the same fate. In this study, two marine invertebrates Scrobicularia plana and Hediste diversicolor were chosen as ecotoxicological models. The aim was to evaluate behavioural (burrowing kinetics, feeding rate) and biochemical (biomarkers) responses of S. plana and H. diversicolor exposed in the laboratory to Cu (10 mu g L(-1)) added in natural seawater either in the form of engineered nanoparticles (NPs) of CuO or as dissolved Cu in 2% HNO(3). Exposure was characterized by considering (i) the physico-chemical fate of NP (ii) the fraction of...
Tipo: Text Palavras-chave: Copper oxide nanoparticles; Behaviour; Biomarkers; Oxidative stress; Scrobicularia plana; Hediste diversicolor.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00040/15140/15856.pdf
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Biochemical-molecular markers in unilateral ureteral obstruction Biocell
Manucha,Walter.
Congenital obstructive nephropathy is the primary cause of end-stagerenal disease in children. Rapid diagnosis and initiation of the treatment are vital to preserve function and/or to slow down renal injury. Obstr uctive uropathy effects-decline in the plasmatic renal flow and glomerular filtrationrate, interstitial infiltrate of leukocytes, significant decrease of the urine concentration, loss of the capacity to concentrate urine as well as fibrosis and apoptosisare a consequence of a variety of factors that work in complex ways and are still not fully understood. Mediators as angiotensin II, transforming growth factor-b (TGF-b) and nitric oxide (NO) have been implicated in congenital obstructive nephropathy. The renin-angiotensin system is regulated in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Unilateral ureteral obstruction; Interstitial fibrosis; Nitric oxide; Angiotensin II; Oxidative stress.
Ano: 2007 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000100001
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Characterization of the oxidative system on Castanea sativa Mill. in association with the ectomycorrhizal fungi Pisolithus tinctorius and Amanita muscaria during the early stages of contact IPB - Escola Superior Agrária
Baptista, Paula; Martins, Anabela; Tavares, Rui Manuel; Lino-Neto, Teresa.
Mycorrhizae are symbiotic associations between soil borne fungi and the root system of higher plants having a beneficial role on plant growth. The fungus receives carbon from plant, while the plant increased nutrient uptake mediated via the fungus. In agroforestry systems, most of the mycorrhizae belong to ectomycorrhizal (ECM) group being the mechanisms controlling its development poorly defined. During arbuscular mycorrhizal (AM) establishment, some evidences suggest that a temporal and spatial activation of different defence mechanisms by plants are activated. The present work pretends to assess the influence of ECM inoculation on the activity of antioxidant enzymes from roots of Castanea sativa Mill., during the early stages of contact. The...
Tipo: ConferenceObject Palavras-chave: Ectomycorrhizal; Oxidative stress; Pisolithus tinctorius; Amanita muscaria; Castanea sativa Mill..
Ano: 2004 URL: http://hdl.handle.net/10198/5720
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Cobalt oxide nanoparticles aggravate DNA damage and cell death in eggplant via mitochondrial swelling and NO signaling pathway Biol. Res.
Faisal,Mohammad; Saquib,Quaiser; Alatar,Abdulrahman A.; Al-Khedhairy,Abdulaziz A.; Ahmed,Mukhtar; Ansari,Sabiha M.; Alwathnani,Hend A.; Dwivedi,Sourabh; Musarrat,Javed; Praveen,Shelly.
BACKGROUND: Despite manifold benefits of nanoparticles (NPs), less information on the risks of NPs to human health and environment has been studied. Cobalt oxide nanoparticles (Co3O4-NPs) have been reported to cause toxicity in several organisms. In this study, we have investigated the role of Co3O4-NPs in inducing phytotoxicity, cellular DNA damage and apoptosis in eggplant (Solanum melongena L. cv. Violetta lunga 2). To the best of our knowledge, this is the first report on Co3O4-NPs showing phytotoxicity in eggplant. RESULTS: The data revealed that eggplant seeds treated with Co3O4-NPs for 2 h at a concentration of 1.0 mg/ml retarded root length by 81.5 % upon 7 days incubation in a moist chamber. Ultrastructural analysis by transmission electron...
Tipo: Journal article Palavras-chave: Cobalt oxide nanoparticles; Nanotoxicity; DNA damage; Apoptosis; Oxidative stress.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100020
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Comparative embryotoxicity and genotoxicity of the herbicide diuron and its metabolites in early life stages of Crassostrea gigas: implication of reactive oxygen species production ArchiMer
Behrens, Daphne; Rouxel, Julien; Burgeot, Thierry; Akcha, Farida.
Herbicides are one of the major classes of pollutants contaminating coastal waters over the world. Among them, diuron (3-(3,4-dichlorophenyl)-1,1-dimethylurea) is a phenylurea herbicide frequently detected in oyster-producing area, known to be toxic for this important exploited non-target species. With the aim to investigate the mechanisms by which diuron displays its toxicity in oyster, the implication of both biotransformation and oxygen reactive species (ROS) production was studied considering embryotoxicity and genotoxicity as endpoints. Comparative embryotoxicity and genotoxicity of diuron and its main metabolites (DCPMU, DCPU and 3,4-DCA) were thus studied on oyster larvae by the embryo-larval bioassay on D larvae and the comet assay on trochophore...
Tipo: Text Palavras-chave: Oyster; Herbicide; Embryotoxicity; Genotoxicity; Oxidative stress; Biotransformation.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00324/43521/43053.pdf
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Compensatory responses induced by oxidative stress in Alzheimer disease Biol. Res.
MOREIRA,PAULA I; ZHU,XIONGWEI; LIU,QUAN; HONDA,KAZUHIRO; SIEDLAK,SANDRA L; HARRIS,PEGGY L; SMITH,MARK A.; PERRY,GEORGE.
Oxidative stress occurs early in the progression of Alzheimer disease, significantly before the development of the pathologic hallmarks, neurofibrillary tangles and senile plaques. In the first stage of development of the disease, amyloid-β deposition and hyperphosphorylated tau function as compensatory responses and downstream adaptations to ensure that neuronal cells do not succumb to oxidative damage. These findings suggest that Alzheimer disease is associated with a novel balance in oxidant homeostasis.
Tipo: Journal article Palavras-chave: Alzheimer disease; Antioxidant therapy; Mitochondria; Oxidative stress; Redox metals.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100002
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Co-operative effect of glutathione depletion and selenium induced oxidative stress on API and NFkB expression in testicular cells in vitro: insights to regulation of spermatogenesis Biol. Res.
SHALINI,SONIA; BANSAL,MOHINDER P.
Previous studies have shown that transcription factors, API and NFkB exert important roles in the process by which selenium regulates spermatogenesis. Glutathione, an intracellular thiol, acts as a source of reducing power and aids in maintenance of the cellular redox status. The activities of selenium are closely related to the availability of glutathione. Presently, mouse testicular cells were cultured in the presence of BSO, a known glutathione depletor, to generate oxidative stress. Selenium (Se) was added as sodium selenite to these cells at concentrations of 0.5 µM and 1.5 µM. It was observed that at 1.5 µM, Se acted as a pro-oxidant and significantly decreased the redox ratio. RT PCR analysis revealed that cjun, cfos expression increased in...
Tipo: Journal article Palavras-chave: API; BSO; GSH; NFkB; Oxidative stress; Selenium; Testicular cells.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400005
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Cytoprotective and suicidal signaling in oxidative stress Biol. Res.
Videla,Luis A.
Oxidative stress is an imbalance between pro-oxidants and antioxidants in favor of the pro-oxidants, leading to different responses depending on the level of pro-oxidants achieved and the duration of exposure. In this article, we discuss the cytoprotective or suicidal signaling mechanisms associated with oxidative stress by addressing: (i) the development of an acute and mild pro-oxidant state by thyroid hormone administration eliciting the redox upregulation of the expression of proteins affording cell protection as a preconditioning strategy against ischemia-reperfusion liver injury; and (ii) the role of prolonged and severe oxidative stress and insulin resistance as determinant factors in the pathogenesis of non-alcoholic fatty liver disease associated...
Tipo: Journal article Palavras-chave: Oxidative stress; Thyroid hormone; Liver preconditioning; Obesity; Insulin resistance; Non-alcoholic fatty liver disease.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300014
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Density and Morphometry of Overall Myenteric Neurons Population from the Duodenum of Diabetic Rats (Rattus norvegicus) under Ascorbic Acid Supplementation International Journal of Morphology
Pereira,Marli Aparecida dos Santos; Liberati,Ana Paula; Souza,Ivan Domicio da Silva; Zanoni,Jacqueline Nelisis.
The objective of this work was to evaluate the overall myenteric neurons population from the duodenum of adult streptozotocin-induced diabetic rats supplemented with ascorbic acid (AA), a potent antioxidant. Fifteen 90-day-old rats were divided in groups: control (C), diabetic (D), diabetic treated with ascorbic acid (DA). After 120 days of experimental period duodenums were resected and processed as whole-mount preparations according to Giemsa's technique, which allowed us to evaluate neuronal density in an area of 8.96 mm² and measure the area of 500 neuronal cell bodies per group. It was observed a 32.55% reduction in neuronal density of group D when compared to group C (p<0.05). The density of spared neurons in group DA, in relation to group D, was...
Tipo: Journal article Palavras-chave: Ascorbic acid; Diabetes mellitus; Duodenum; Oxidative stress.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000200017
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Diabetes-Induced Prefrontal Nissl Substance Deficit and the Effects of Neem-Bitter Leaf Extract Treatment International Journal of Morphology
Akinola,Oluwole B; Omotoso,Olaiya G; Dosumu,Olufunke O; Akinola,Oluwafunmike S; Olotufore,Favour.
Cognitive dysfunction is reportedly associated with poorly-managed diabetes mellitus. In this study, we report the effect of oral treatment with combined leaf extract (CLE) of neem and bitter leaf on the prefrontal cortex of diabetic Wistar rats. Adult male Wistar rats were randomized to one of the following groups: control, diabetic (STZ-induced), STZ + CLE, STZ + metformin and CLE only. At euthanasia, paraffin sections of the prefrontal cortex were stained with cresyl fast violet; while malondialdehyde (MDA) and glutathione peroxidase (GPx) were assayed in prefrontal homogenates. Oral CLE produced normoglycemia in the treated hyperglycaemic rats. Besides, Nissl-stained prefrontal sections showed no morphologic deficits in all the groups except the...
Tipo: Journal article Palavras-chave: Pyramidal cells; Hyperglycaemia; Oxidative stress; Cognition.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300031
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Discrimination of population of recombinant inbred lines of rye (Secale cereale L.) for different responses to nitrogen-potassium stress assessed at the seedling stage under in vitro conditions Electron. J. Biotechnol.
Smolik,Mi&#322;osz.
Background: Plants differ in the methods used to acquire nutrients from environments with low nutrient availability, and may change the morphology of their ‘root architecture’ to be able to take up nutrients. Results: In the present study rye response to stress caused by high and low nitrogen-potassium treatments in mature embryos cultures was described within a population consisting of one hundred and thirty eight recombinant inbred lines of rye. Characterization of the response of recombinant inbred lines (RILs) to nutrient stress was presented as the results of analyses of morphological traits, and physiological and biochemical parameters of the seedlings grown in both treatments. A wide range of variability of individual RILs to induced stress was...
Tipo: Journal article Palavras-chave: Mature embryos; Nutrient stress; Oxidative stress; RILs; Rye.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000100005
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Drought tolerance and antioxidant enzymatic activity in transgenic ?Swingle? citrumelo plants over-accumulating proline. Repositório Alice
CAMPOS, M. K. F. de; CARVALHO, K. de; SOUZA, F. S. de; MARUR, C. J.; PEREIRA, L. F. P.; BESPALHOK FILHO, J. C.; VIEIRA, L. G. E..
In this study we investigated the effects of the high endogenous proline level on water relations, gas exchange and antioxidant enzymatic activity in leaves of transgenic ?Swingle? citrumelo rootstocks transformed with the P5CSF129A gene coding for the key-enzyme for proline synthesis, under water deficit. Leaf total water, osmotic and pressure potentials, stomatal conductance, photosynthetic rates and xylem sapflowwere evaluated in non-transformed control and transgenic plants during water deficit treatment. Malondialdehyde (MDA) content, catalase (CAT; EC 1.11.1.6), superoxide dismutase (SOD; EC 1.15.1.1) and ascorbate peroxidase (APX; EC 1.11.1.11) activities were quantified in leaves collected based on their total water potential, representing the...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Citrus rootstock; Drought tolerance; Osmotic adjustment; Oxidative stress; Proline.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/902435
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Effect of dietary probiotic Pediococcus acidilactici on antioxidant defences and oxidative stress status of shrimp Litopenaeus stylirostris ArchiMer
Castex, Mathieu; Lemaire, Pierrette; Wabete, Nelly; Chim, Liet.
in this study we evaluated the effects of the probiotic Pediococcus acidilactici MA18/5M on shrimp, Litopenaeus stylirostris (also called Penaeus stylirostris), first on antioxidant defences and secondly on the oxidative stress status in the shrimps' haemolymph and digestive gland. We conducted two experiments with the same protocol in which shrimps were fed two diets for three weeks: a control diet and a probiotic diet containing 1 g of live P. acidilactici MA18/5M kg(-1). In the first experiment, the shrimps were found to be healthy over the trial period; no mortalities and non-detectable signs of infection were recorded. These resulted in high final survival rates (above 90% in both treatments). On the other hand, during the second trial, carried out at...
Tipo: Text Palavras-chave: Vibriosis; Antioxidant defences; Oxidative stress; Probiotic; Shrimps.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6780.pdf
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