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Registros recuperados: 10
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A novel proteomic approach identifies new interaction partners for proliferating cell nuclear antigen ArchiMer
Mesiet Cladiere, L; Norais, Cédric; Kuhn, Joelle; Briffotaux, Julien; Sloostra, Jerry W.; Ferrari, Elena; Hubscher, Ulrich; Flament, Didier; Myllykallio, Hannu.
During DNA replication and repair, many proteins bind to and dissociate in a highly specific and ordered manner from proliferating cell nuclear antigen (PCNA). We describe a combined approach of in silico searches at the genome level and combinatorial peptide synthesis to investigate the binding properties of hundreds of short PCNA-interacting peptides (PIP-peptides) to archaeal and eukaryal PCNAs. Biological relevance of our combined approach was demonstrated by identification an inactive complex of Pyrococcus abyssi ribonuclease HII with PCNA. Furthermore we show that PIP-peptides interact with PCNA largely in a sequence independent manner. Our experimental approach also identified many so far unidentified PCNA interacting peptides in a number of human...
Tipo: Text Palavras-chave: Ribonuclease HII; Combinatorial peptide synthesis; PCNA.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3297.pdf
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alpha-Smooth muscle actin and proliferating cell nuclear antigen expression in focal segmental glomerulosclerosis: functional and structural parameters of renal disease progression BJMBR
Geleilete,T.J.M.; Costa,R.S.; Dantas,M.; Coimbra,T.M..
The aim of the present study was to investigate the expression of alpha-smooth muscle actin (alpha-SM-actin) and proliferating cell nuclear antigen (PCNA) in renal cortex from patients with focal segmental glomerulosclerosis (FSGS) and their correlations with parameters of renal disease progression. We analyzed renal biopsies from 41 patients with idiopathic FSGS and from 14 control individuals. The alpha-SM-actin immunoreaction was evaluated using a score that reflected the changes in the extent and intensity of staining in the glomerular or cortical area. The PCNA reaction was quantified by counting the labeled cells of the glomeruli or renal cortex. The results, reported as median ± percentile (25th; 75th), showed that the alpha-SM-actin scores in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alpha-smooth muscle actin; Proliferating cell nuclear antigen; PCNA; Focal segmental glomerulosclerosis; Proteinuria; Renal failure; Renal disease progression; Renal fibrosis.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800003
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Analysis of p53 expression and proliferative assessment using PCNA in localized prostate carcinoma BJMBR
Leite,K.R.M.; Srougi,M.; Nesralhah,L.J.; Camara-Lopes,L.H..
The surgical specimens from 51 men submitted to radical prostatectomy for localized prostate cancer were examined by immunohistochemistry using proliferation cell nuclear antigen (PCNA) monoclonal antibody to evaluate the proliferative index (PI). The relationship between PI, biological variables and p53 protein expression was evaluated by immunohistochemistry. PI was low in invasive localized prostate carcinoma (mean, 12.4%) and the incidence of PCNA-positive cells was significantly higher in tumors with p53 expression (P = 0.0226). There was no statistical difference in PCNA values when biological parameters such as Gleason score, tumor volume, extraprostatic involvement, seminal vesicle infiltration or lymph node metastasis were considered. We conclude...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Prostate carcinoma; P53; Proliferating cell nuclear antigen; PCNA.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300006
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Avaliação histomorfométrica e da proliferação celular em úlceras de córnea superficiais, induzidas em coelhos, após o uso de colírio de Citrus lemon Arq. Bras. Med. Vet. Zootec.
Perches,C.S.; Pellizzon,C.H.; Ranzani,J.J.T.; Padovani,C.R.; Merlini,N.B.; Sereno,M.G.; Fonzar,J.F.; Brandão,C.V.S..
O objetivo do presente estudo foi avaliar e comparar, por meio de histomorfometria e imuno-histoquímica para PCNA (Antígeno Nuclear de Proliferação Celular), o processo de reparação corneal de úlceras superficiais induzidas em coelhos e tratadas com colírios de óleo essencial de Citrus lemon (CL). Foram utilizadas 40 fêmeas da espécie leporina, constituindo-se quatro grupos experimentais de 10 animais cada. Todos os animais foram submetidos à indução da úlcera superficial experimental por meio da aplicação tópica de n-heptanol. Em dois grupos foram instilados colírios à base de óleo essencial de Citrus lemon, em diferentes concentrações, sendo 3% (GL3) e 5% (GL5). Outro grupo foi tratado com Tween 80 8% (GT), que é o diluente utilizado na produção dos...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Limão-siciliano; Córnea; Úlcera; PCNA; Histologia.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000601660
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Inmunohistochemical Expression of PCNA, p53 and bcl-2 in Pleomorphic Adenomas International Journal of Morphology
Gordón-Núñez,Manuel Antonio; Godoy,Gustavo Pina; Soares,Rosilene Calazans; Souza,Lélia Batista de; Freitas,Roseana de Almeida; Queiroz,Lélia María Guedes.
The aim of the study was to determine the immunohistochemical expression of the PCNA, p53 and bcl-2 proteins in pleomorphic adenomas.onineteen specimens of pleomorphic adenomas were selected for analysis by the streptavidin-biotin-peroxidase method with antibodies againstp53, PCNA and bcl-2 proteins. It was observedweak labeling for p53 in 12 cases (63.1%) andforPCNA in 8 (42.1%). With respect to the bcl-2 labeling index,ono expression of this protein was detected in 12 cases, corresponding to 63.1% of the sample. Based on these findings, it was concluded that p53 and PCNA can favor the proliferative activity of pleomorphic adenomas, whereas bcl-2 probably doesonot effectively particípate in the pathogenesis of this tumor.
Tipo: Journal article Palavras-chave: Pleomorphic adenoma; Salivary gland tumour; P53; PCNA; Bcl-2.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000300010
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La réplication de l'ADN chez l'euryarchaea Pyrococcus Abyssi : mise en place et dynamique du complexe ArchiMer
Rouillon, Christophe.
DNA replication occurs through a large protein complex assembly called replisome. An understanding of its structure and function requires its in vitro reassembly from individual subunits. In all living organisms, the elongation of a primed DNA template by DNA polymerase, with high processivity, requires the accessory protein DNA sliding clamp (PCNA) that is loaded onto DNA by the clamp loader (RF-C). Generally, the archaeal proteins involved in DNA replication are more similar to those of the higher organisms (Eukarya) rather than to those from Bacteria. This study have focussed on the PCNA loading mechanism and on the DNA synthesis in the hyperthermophilic Euryarchaea Pyrococcus abyssi. To investigate the importance of some protein-protein and...
Tipo: Text Palavras-chave: Pyrococcus; Archaea; DNA synthesis; Interaction; RP A; RF C; PCNA; DNA polymerase; DNA replication; Pyrococcus; Archée; Archaea; Synthese d'ADN; Interaction; RP A; RF C; PCNA; ADN polymerase; Replication de l'ADN.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/these-2461.pdf
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Les ADN polymérases B et D de l'Archaea hyperthermophile, Pyrococcus abyssi : contribution à l'étude des relations structure-fonction ArchiMer
Castrec, Benoit.
DNA replication is a functionally conserved mechanism among the three domains of life. This processus is performed by DNA polymerases with two accessories factors, PCNA and RF-C, sliding clamp and clamp loader, respectively. Our model of study, the Euryarchaeota Pyrococcus abyssi, possesses one monomeric DNA polymerase, belonging to the Family B, and one heterodimeric DNA polymerase, belonging to the Family D. Usually, proteins interact with PCNA at a hydrophobic pocket which is formed by the IDCL (interdomain connecting loop) and the C-terminus. These interactions are mediated through common motifs like the PIP box (motif PCNA interacting Protein). We have demonstrated that Pol B has just one PIP box motif at the C-terminus, while Pol D has two PIP-type...
Tipo: Text Palavras-chave: Thermostability; Interactions; Motifs; DNA replication; RF C; PCNA; DNA polymerases; Archaea; Thermostabilité; Interactions; Motifs; Réplication de l'ADN; RF C; PCNA; ADN polymérases; Archaea.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/these-6940.pdf
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Marcadores de proliferação celular na avaliação do crescimento do tumor sólido e ascítico de Ehrlich Arq. Bras. Med. Vet. Zootec.
Silva,A.E.; Santos,F.G.A.; Cassali,G.D..
Twenty BALB/c mice were inoculated with cell suspension of Ehrlich tumor. Ten mice were inoculated in the cushion plant (solid form) and the other 10 in the peritoneum (ascitic form). Animals were euthanized on different times (7 and 14 days). Cytological and histological slides, immunohistochemical (PCNA) analysis and NORs silver impregnation technique were performed. The results showed more proliferation on the 7th day in the ascitic form and on 14th day in the solid form, using both analyses (PCNA and AgNORs). The alterations observed in the Ehrlich tumor’s proliferation activity suggested that the growth curve is different between ascitic and solid forms. In the first one, the proliferation peak occurs on the seventh day and in the solid tumor the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Camundongo; Tumor de Ehrlich; AgNOR; PCNA.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000400030
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Phytohemagglutinin improves the development and ultrastructure of in vitro-cultured goat (Capra hircus) preantral follicles BJMBR
Cunha,E.V.; Costa,J.J.N.; Rossi,R.O.D.S.; Silva,A.W.B.; Passos,J.R.S.; Portela,A.M.L.R.; Pereira,D.C.S.T.; Donato,M.A.M.; Campello,C.C.; Saraiva,M.V.A.; Peixoto,C.A.; Silva,J.R.V.; Santos,R.P..
The objective this study was to determine the effect of phytohemagglutinin (PHA) on survival, growth and gene expression in caprine secondary follicles culturedin vitro. Secondary follicles (∼0.2 mm) were isolated from the cortex of caprine ovaries and cultured individually for 6 days in α-MEM+ supplemented with PHA (0, 1, 10, 50, 100, or 200 µg/mL). After 6 days of culture, follicle diameter and survival, antrum formation, ultrastructure and expression of mRNA for FSH receptors (FSH-R), proliferating cell nuclear antigen (PCNA), and neuronal nitric oxide synthase were determined. All treatments maintained follicular survival [α-MEM+ (94.59%); 1 µg/mL PHA (96.43%); 10 µg/mL PHA (84.85%); 50 µg/mL PHA (85.29%); 100 µg/mL PHA (88.57%), and 200 µg/mL PHA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Caprine; Antrum formation; FSH-R; PCNA; Ultrastructure.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300245
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The PCNA from Thermococcus fumicolans functionally interacts with DNA polymerase delta ArchiMer
Henneke, Ghislaine; Raffin, Jean-paul; Ferrari, Elena; Jonsson, Zophonías; Dietrich, Jacques; Hubscher, Ulrich.
We have cloned the gene encoding proliferating cell nuclear antigen (PCNA) from the hyperthermophilic euryarchaeote Thermococcus fumicolans (Tfu). Tfu PCNA contains 250 amino acids with a calculated M-r of 28,000 and is 26% identical to human PCNA. Next, Tfu PCNA was overexpressed in Escherichia coli and it showed an apparent molecular mass of 33.5 kDa. The purified Tfu PCNA was tested first with recombinant Tfu DNA polymerase I (Tfu pol) and second with calf thymus DNA polymerase delta (pol delta). When tested with the homologous Tfu pol on bacteriophage lambda DNA, large amounts of Tfu PCNA were required to obtain two- to threefold stimulation. Surprisingly, however, Tfu PCNA was much more efficient than human PCNA in stimulating calf thymus pol delta....
Tipo: Text Palavras-chave: DNA polymerase delta; Conservation; Thermococcus fumicolans; Archaea; Hyperthermophile; PCNA.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-659.pdf
Registros recuperados: 10
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