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AISLAMIENTO Y CARACTERIZACIÓN DE UNA POLIFENOLOXIDASA RELACIONADA CON LA TOLERANCIA DEL CLAVEL (Dianthus caryophyllus) A Fusarium oxysporum f. sp. dianthi raza 2 Acta biol.Colomb.
ROQUESA MAYORGA,VIANA; HIGUERA M,BLANCA LIGIA.
Se purificó y caracterizó bioquímicamente la enzima polifenoloxidasa (PFO) inducida en tallos de clavel (Dianthus caryophyllus L) de variedad tolerante por inoculación con el patógeno Fusarium oxysporum f. sp. dianthi raza 2 (FOD2), causante del marchitamiento vascular. La purificación se logró a través de procesos sucesivos de cromatografía en columna de intercambio iónico, de interacción hidrofóbica y de exclusión molecular. A través de éstos se logró un factor de purificación de 314 veces con respecto al extracto inicial. La proteína purificada mostró actividad PFO y una única banda en SDSPAGE correspondiente a 40 kDa. Usando catecol como sustrato, se determinó su temperatura óptima en 45 ºC y su pH óptimo en 7,5. La enzima presentó una cinética tipo...
Tipo: Journal article Palavras-chave: Polifenoloxidasa; PPO; Clavel; Proteínas purificación.
Ano: 2007 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2007000200007
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Aloe vera extract as a promising treatment for the quality maintenance of minimally-processed table grapes Ciênc. Tecnol. Aliment.
Alberio,Giuseppina Rosaria Antonella; Muratore,Giuseppe; Licciardello,Fabio; Giardina,Giovanni; Spagna,Giovanni.
The effect of an edible film obtained from a commercial Aloe vera extract, on the quality maintenance of minimally processed grapes belonging to three different cultivars (Sugar One, Victoria and Black Magic) was evaluated by enzymatic (PPO, PME, β-GAL), physicochemical (pH, acidity, °Brix), and sensorial methods. All the analyzed parameters were measured in extracts obtained from minimally processed grapes packaged in ordinary atmosphere and stored at 4 °C for 15 days. Samples dipped into Aloe vera showed significant differences (p≤0.05) compared to untreated ones. The determination of such parameters and the evaluation of consumer acceptability were helpful to determine the effectiveness of the post-harvest treatment with Aloe vera for a storage period...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aloe vera; Packaging; PPO; Shelf life; Table grape.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612015000200299
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Effect of pH and substrates on polyphenol oxidase activity of Japanese processing potatoes OAK
Hironaka, Kazunori; Itaya, Toshihiko; Koaze, Hiroshi; Yamamoto, Kazuo; Sato, Tadatoshi; Kojima, Michiyuki; Mori, Motoyuki; Tsuda, Shogo; 弘中, 和憲; 板谷, 俊彦; 小疇, 浩; 山本, 和夫; 佐藤, 禎稔; 小嶋, 道之; 森, 元幸; 津田, 昌吾.
Palavras-chave: ジャガイモ; ポリフェノールオキシダーゼ; PH; 基質; Potato tuber; PPO; Substrate.
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2270
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Effect of polyphenol oxidase (PPO) and air treatments on total phenol and tannin content of cocoa nibs Ciênc. Tecnol. Aliment.
Brito,Edy Sousa de; García,Nelson Horacio Pezoa; Amâncio,Allan César.
Cocoa flavour is greatly influenced by polyphenols. These compounds undergo a series of transformations during cocoa processing leading to the characteristic cocoa flavour. The use of exogenous polyphenol oxidase (PPO) proved to be useful to reduce polyphenol content in cocoa nibs. The effect of a PPO associated or not with air over total phenol and tannin content was evaluated. Cocoa nibs were autoclaved and treated with a PPO or water in the absence or presence of an air flow for 0.5, 1, 2 and 3 hours. Total phenol content was reduced in PPO or water treatments, but when associated with air there was an increase in phenol content. Tannin content was reduced only by the treatment with water and air.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cocoa; Processing; PPO; Enzyme; Air; Theobroma cacao.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612002000100008
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