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Registros recuperados: 14
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Anopheles gambiae PRS1 modulates plasmodium development at both midgut and salivary gland steps Inra
Chertemps, T.; Mitri, C.; Perrot, S.; Sautereau, J.; Jacques, J.-C.; Thiery, I.; Bourgouin, C.; Rosinski-Chupin, I..
Background: Invasion of the mosquito salivary glands by Plasmodium is a critical step for malaria transmission. From a SAGE analysis, we previously identified several genes whose expression in salivary glands was regulated coincident with sporozoite invasion of salivary glands. To get insights into the consequences of these salivary gland responses, here we have studied one of the genes, PRS1 (Plasmodium responsive salivary 1), whose expression was upregulated in infected glands, using immunolocalization and functional inactivation approaches. Methodology/Principal Findings: PRS1 belongs to a novel insect superfamily of genes encoding proteins with DM9 repeat motifs of uncharacterized function. We show that PRS1 is induced in response to Plasmodium, not...
Tipo: Journal Article Palavras-chave: PLASMODIUM; MOUSTIQUE; GLANDE SALIVAIRE; SALIVE; MALADIE INFECTIEUSE; PROTEINE; TRANSMISSION DE LA MALADIE; VECTEUR DE LA MALADIE; INTERACTION MOLECULAIRE; RT-PCR; SDS-PAGE; ARTHROPODA; HEXAPODA; EXPRESSION DES GENES; MICROSCOPIE CONFOCALE; INSECTE; PARASITOSE MALARIA PARASITE; MOLECULAR-INTERACTIONS; BERGHEI SPOROZOITES; MOSQUITO; INVASION; INFECTION; PROTEIN; VECTOR; CELLS; FALCIPARUM.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010f5c08798&uri=/notices/prodinra1/2010/11/
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Biochemical approach to muscular function in insects National Institute of Agronomic Research
Maruyama, K..
Tipo: Journal Article-postprint Palavras-chave: INSECT; MUSCLE; FUNCTION; BIOCHEMISTRY; PROTEIN; ACTOMYOSIN; ATP; INSECTE; MUSCLE ALAIRE; FONCTION; BIOCHIMIE; PROTEINE; ACTOMYOSIN; ADENOSINE TRIPHOSPHATE.
Ano: 1957 URL: http://hdl.handle.net/2174/469
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Dairy powder rehydration: influence of protein state, incorporation mode, and agglomeration Inra
Gaiani, C.; Schuck, P.; Scher, J.; Desobry, S.; Banon, S..
A simplified method to study rehydration was used on different dairy powders. The method involved dispersing powder in a stirred vessel equipped with a turbidity sensor. The changes of turbidity occurring during powder rehydration highlighted the rehydrationstage, and the influence of the proteins’ state on rehydration was clarified. Casein powders had a quick wetting time but very slow dispersion, making the totalrehydration process time-consuming. On the other hand, whey powders were found to have poor wettability but demonstrated immediate dispersion after wetting.Mixing casein (80%) and whey (20%) before spray drying greatly improved rehydration time compared with casein powder; whereas mixing whey powder with casein powder at the same ratio after...
Tipo: Journal Article Palavras-chave: REHYDRATION; POWDER; PROTEIN; AGGLOMERATION.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20081f9e4fa8&uri=/notices/prodinra1/2008/02/
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Designing of a wheat gluten/montmorillonite based system as carvacrol carrier: Rheological and structural properties Inra
Mascheroni, E.; Chalier, P.; Gontard, N.; Gastaldi, E..
An antimicrobial delivery system was designed from film-forming solutions containing wheat gluten 20% (wt/vol) (WG) as matrix, montmorillonites (Mmt) as structuring agent and carvacrol as active agent (15% wt/wt dry WG). The objectives of this work were to understand how the addition of montmorillonites (0-10% wt/wt dry WG) affects the carvacrol retention capacity of both the film forming solution and the resulting film obtained by casting. For this purpose, structural, granulometric and rheological changes that occurred in the film-forming solutions were studied. The introduction of carvacrol in a WG solution containing increasing amounts of Mmt resulted in dramatic changes in its rheological behaviour. Depending on the Mmt amount added (<or >=5%),...
Tipo: Journal Article Palavras-chave: PROTEIN; AROMA COMPOUNDS; LAYERED SILICATES; RHEOLOGY; GRANULOMETRY; RETENTION.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010cb530085&uri=/notices/prodinra1/2010/12/
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Diet effects on honeybee immunocompetence Inra
ALAUX, C.; Ducloz, F.; Crauser, D.; Le Conte, Y..
The maintenance of the immune system can be costly, and a lack of dietary protein can increase the susceptibility of organisms to disease. However, few studies have investigated the relationship between protein nutrition and immunity in insects. Here, we tested in honeybees (Apis mellifera) whether dietary protein quantity (monofloral pollen) and diet diversity (polyfloral pollen) can shape baseline immunocompetence (IC) by measuring parameters of individual immunity (haemocyte concentration, fat body content and phenoloxidase activity) and glucose oxidase (GOX) activity, which enables bees to sterilize colony and brood food, as a parameter of social immunity. Protein feeding modified both individual and social IC but increases in dietary protein quantity...
Tipo: Journal Article Palavras-chave: INSECTE SOCIAL; SYSTEME IMMUNITAIRE; REGIME ALIMENTAIRE; RESSOURCE ALIMENTAIRE SOCIAL INSECT; HONEYBEE; DIET; POLLEN; POLYFLORAL POLLEN; PROTEIN; DISEASE; IMMUNOCOMPETENCE.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20109faf5aef&uri=/notices/prodinra1/2010/11/
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Domestication and nutrition in fish ArchiMer
Cahu, Chantal.
The aim of this paper is to evaluate to what extent, the study of feeding regimes of fish could be valuable to indicate the possibilities and the constraints of their domestication. Though the natural feeding regime of fish seems to be disadvantageous for their domestication, as protein requirement is high, different species could be considered as domesticated. These species are herbivorous, detritivorous, omnivorous as well as carnivorous. In the same way, there are large differences in gut anatomy, but domestication concerns species with or without stomach, and species with short or very long intestine. Nutritional requirements do not allow predicting species that could be domesticated. Some species seem to be able to use vegetable protein in...
Tipo: Text Palavras-chave: TROUT ONCORHYNCHUS MYKISS; TURBOT PSETTA MAXIMA; RAINBOW TROUT; TOTAL REPLACEMENT; FLESH QUALITY; LIVE FOOD; DIET; PROTEIN; GROWTH; LARVAE.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/00000/11021/7571.pdf
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Etude de différents stress chez l’abeille : le cas des glandes hypopharyngiennes Inra
Baldy, A..
Depuis quelques années nous avons pu voir une hécatombe des abeilles et nos différentesexpériences ont tenté de répondre au problème de la mortalité des abeilles (Apis mellifera). Apartir de ce constat, nous avons voulu tester les effets de quelques stress sur différentsparamètres notamment sur les glandes hypopharyngiennes qui sont importantes pour nourrirles larves. Lors de notre première expérience, nous avons testé la mortalité des abeilles face àdifférents paramètres tels qu’un pesticide, l’imidaclopride et une microspore nomméeNosema. Grâce à cette expérience nous avons observé que ces deux paramètres avaient uneinfluence sur la mortalité observée. Par la suite, nous avons voulu examiner ce qui se passé auniveau des glandes hypopharyngiennes, si nous...
Tipo: Thesis Palavras-chave: ABEILLE DOMESTIQUE; INSECTE SOCIAL; MORTALITE; GLANDE HYPOPHARYNGIENNE; TAILLE; PROTEINE; INSECTICIDE; IMIDACLOPRIDE; NEONICOTINOIDE; STRESS; MICROSPORIDIE HONEYBEE; SOCIAL INSECT; MORTALITY; HYPOPHARYNGEAL GLAND; SIZE; PROTEIN; IMIDACLOPRID; NEONICOTINOID; MICROSPRORIDIA.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010df18f33f&uri=/notices/prodinra1/2010/12/
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Influence of storage on the nutritive value of pollen for brood rearing by honeybees National Institute of Agronomic Research
Haydak, M.H..
Small colonies of emerged bees were kept in confinement, and fed fresh pollen, or pollen two years old supplemented with: nothing, pantothenic acid, 20% dried brewers yeast, 20% vitamin-free casein, casein plus pantothenic acid. The number of sealed brood cells in these colonies (in the above order) was 8-9,l-0,2-8,4-4,5-6, and 5-8 times the number in the colonies fed old pollen alone. Deterioration of proteins and of pantothenic acid is thus partially responsible for the inferior value of stored pollen for brood rearing.
Tipo: Journal Article-postprint Palavras-chave: POLLEN; STORAGE; FOOD VALUE; HONEYBEE; BROOD; PROTEIN; POLLEN; STOCKAGE; VALEUR NUTRITIVE; ABEILLE; APIS MELLIFERA; APIDAE; COUVAIN; PROTEINE; ACIDE PANTOTHENIQUE.
Ano: 1963 URL: http://hdl.handle.net/2174/519
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Phenotypic and genetic statistics of components of milk and two measures of somatic cell concentration Inra
Manfredi, E.; Everett, R.W.; Searle, R..
Tipo: Journal Article Palavras-chave: HERITABILITIES; GENOTYPIC; PHENOTYPIC; MILK; LACTATION SCORE; CONCENTRATION; PROTEIN.
Ano: 1984 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010db49ab62&uri=/notices/prodinra1/2010/03/
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Proteome changes in leaves from grapevine (Vitis vinifera L.) transformed for alcohol dehydrogenase activity Inra
Sauvage, F.X.; Pradal, M.; Chatelet, P.; Tesniere, C..
A proteomic approach has been used to study changes in leaf protein content from plants transformed for alcohol dehydrogenase (ADH) activity. Individual quantitative analysis of 190-436 spots separated by two-dimensional electrophoresis was performed, and spots displaying significant quantitative changes between control (C), sense (S), and antisense (R) transformants were selected using Student's t test. Of the 14 spots selected and further analyzed after trypsic digestion, 9 could be identified by MS analysis and 5 by LC-MS/MS. Identified proteins had mainly a chloroplastic origin: four rubisco large subunits, one rubisco binding protein, two glutamine synthetases, one elongation factor Tu, one ATP synthase beta subunit, and one plastidic aldolase....
Tipo: Journal Article Palavras-chave: ALCOHOL DEHYDROGENASE; GRAPEVINE; LEAVE; PROTEIN; TRANSFORMANT.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008425bb395&uri=/notices/prodinra1/2009/02/
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Regeneration mechanisms of arabidopsis thaliana methionine sulfoxide reductases B by glutaredoxins and thioredoxins Inra
Tarrago, L.; Laugier, E.; Zaffagnini, M.; Marchand, C.; Le Maréchal, P.; Rouhier, N.; Lemaire, S.D.; Rey, P..
Methionine oxidation leads to the formation of S- and R-diastereomers of methionine sulfoxide (MetSO), which are reduced back to methionine by methionine sulfoxide reductases (MSRs) A and B, respectively.MSRBs are classified in two groups depending on the conservation of one or two redox-active Cys: 2-Cys MSRBs possess a catalytic Cys reducing MetSO and a resolving Cys allowing regeneration by thioredoxins (Trxs). The second type, 1-Cys MSRBs, possess only the catalytic Cys. The biochemical mechanisms involved in activity regeneration of 1-Cys MSRBs remain largely elusive. In the present work, we used recombinant plastidial Arabidopsis thaliana MSRB1 and MSRB2, as models for 1-Cys and 2-Cys MSRBs, respectively, to delineate the Trx- and...
Tipo: Journal Article Palavras-chave: ARABIDOPSIS THALIANA; METHIONINE; REGENERATION; ESCHERICHIA-COLI; BIOCHEMICAL-CHARACTERIZATION; S-GLUTATHIONYLATION; CATALYTIC MECHANISM; REDOX REGULATION; REDUCING AGENT; ACTIVE-SITE; PROTEIN; PEROXIREDOXIN; POPLAR.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010bd5e65f0&uri=/notices/prodinra1/2010/10/
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Structural deformation upon protein-protein interaction: A structural alphabet approach Inra
Martin, J.; Regad, L.; Lecornet, H.; Camproux, A.C..
BackgroundIn a number of protein-protein complexes, the 3D structures of bound and unbound partners significantly differ, supporting the induced fit hypothesis for protein-protein binding.ResultsIn this study, we explore the induced fit modifications on a set of 124 proteins available in both bound and unbound forms, in terms of local structure. The local structure is described thanks to a structural alphabet of 27 structural letters that allows a detailed description of the backbone. Using a control set to distinguish induced fit from experimental error and natural protein flexibility, we show that the fraction of structural letters modified upon binding is significantly greater than in the control set (36% versus 28%). This proportion is even greater in...
Tipo: Journal Article Palavras-chave: PROTEIN; 3D STRUCTURE; BIOINFORMATIC; LOCAL STRUCTURE; PROTEIN COMPLEX; PROTEIN-PROTEIN INTERATION; STRUCTURAL ALPHABET; HIDDEN MARKOV MODEL.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008778a9939&uri=/notices/prodinra1/2009/11/
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The bdbDC operon of bacillus subtilis encodes thiol-disulfide oxidoreductases required for competence development Inra
Meima, R.; Eschevins, C.; Fillinger, S.; Bolhuis, A.; Hamoen, L.W.; Dorenbos, R.; Quax, W.J.; van Dijl, J.M.; Provvedi, R.; Chen, I.; Dubnau, D.; Bron, S..
The development of genetic competence in the Gram-positive eubacterium Bacillus subtilis is a complex postexponential process. Here we describe a new bicistronic operon, bdbDC, required for competence development, which was identified by the B. subtilis Systematic Gene Function Analysis program. Inactivation of either the bdbC or bdbD genes of this operon results in the loss of transformability without affecting recombination or the synthesis of ComK, the competence transcription factor. BdbC and BdbD are orthologs of enzymes known to be involved in extracytoplasmic disulfide bond formation. Consistent with this, BdbC and BdbD are needed for the secretion of theEscherichia coli disulfide bond-containing alkaline phosphatase, PhoA, by B. subtilis....
Tipo: Journal Article Palavras-chave: BACTÉRIE GRAM NÉGATIF; EXPRESSION DES GÈNES; OPERON; OXYDOREDUCTASE THIOLDISULFIDE; DÉVELOPPEMENT; FONCTION; GÉNÉTIQUE BACTÉRIENNE; SÉQUENCE NUCLÉOTIDIQUE; ACTIVITÉ ENZYMATIQUE ESCHERICHIA-COLI; BOND FORMATION; TRANSCRIPTION FACTOR; MOLECULAR-CLONING; GENOME SEQUENCE; DNA-BINDING; PROTEIN; GENE; IDENTIFICATION; TRANSFORMATION.
Ano: 2002 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0400023579103391&uri=/notices/prodinra1/2010/10/
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The FeFe hydrogenase of Nyctotherus ovalis has a chimeric origin Inra
Boxma, B.; Ricard, G.; van Hoek, A. H.; Severing, E.; Moon-van der Staay, S.Y.; van der Staay, G.W.M.; van Alen, T.A.; de Graaf, R.M.; Cremers, G.; Kwantes, M.; McEwan, N.R.; Newbold, C.J.; Jouany, J.P.; Michalowski, T.; Pristas, P.; Huynen, M.A.; Hackstein, J.H.P..
Background: The hydrogenosomes of the anaerobic ciliate Nyctotherus ovalis show how mitochondria can evolve into hydrogenosomes because they possess a mitochondrial genome and parts of an electron-transport chain on the one hand, and a hydrogenase on the other hand. The hydrogenase permits direct reoxidation of NADH because it consists of a [ FeFe] hydrogenase module that is fused to two modules, which are homologous to the 24 kDa and the 51 kDa subunits of a mitochondrial complex I.Results: The [ FeFe] hydrogenase belongs to a clade of hydrogenases that are different from well-known eukaryotic hydrogenases. The 24 kDa and the 51 kDa modules are most closely related to homologous modules that function in bacterial [ NiFe] hydrogenases. Paralogous,...
Tipo: Journal Article Palavras-chave: MITOCHONDRIE; CILIE; HYDROGENASE MULTIPLE SEQUENCE ALIGNMENT; COMPLEX-I; PHYLOGENETIC ANALYSIS; EUKARYOTIC EVOLUTION; IRON HYDROGENASES; LIFE-STYLE; HYDROGENOSOMES; MITOCHONDRIA; PROTEIN; MODELS.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008786cfe28&uri=/notices/prodinra1/2009/10/
Registros recuperados: 14
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