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Registros recuperados: 74
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2.2.3.3. La phosphatase cytoplasmique dépendant du Mg++ IRD
Souciet, G..
Tipo: Text Palavras-chave: LATEX; ENZYME; PURIFICATION; HEVEA BRASILIENSIS; PHOSPHATASE NEUTRE; SERUM CYTOPLASMIQUE; CARACTERISTIQUE BIOCHIMIQUE.
Ano: 1977 URL: http://www.documentation.ird.fr/hor/fdi:20244
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A lactic acid bacterium with potential application in cassava fermentation IRD
Giraud, Eric; Brauman, Alain; Kéléke, S.; Gosselin, Laurent; Raimbault, Maurice.
An amylolytic lactic acid bacterium, identified as #Lactobacillus plantarum$, was isolated from cassava roots (#Manihot esculenta$ var. Ngansa) during retting. Cultured on starch, the strain displayed a growth rate of 0.43 per hour, a biomass yield of 0.19 g/g, and a lactate yield of 0.81 g/g. The growth kinetics were similar on starch and glucose. Enough enzyme was synthesized, and starch hydrolysis was not a limiting factor for growth. The synthesized amylolytic enzyme was purified by fractionated precipitation with ammonium sulfate and by anion exchange chromatography. It was identified as an alpha-amylase with an optimal pH of 5.5 and an optimal temperature of 65°C. The use of such a strain as a cassava fermentation starter for gari production had the...
Tipo: Text Palavras-chave: FERMENTATION; AMIDON; GLUCOSE; MICROBIOLOGIE; BACTERIE; MILIEU DE CULTURE; ENZYME; PURIFICATION; PH; TEMPERATURE; ACTIVITE ENZYMATIQUE; AMYLASE; ACIDE LACTIQUE.
Ano: 1996 URL: http://www.documentation.ird.fr/hor/fdi:010008964
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A new method of purification of proteasome substrates reveals polyubiquitination of 20 S proteasome subunits Inra
Ventadour, S.; Jarzaguet, M.; Wing, S.S.; Chambon, C.; Combaret, L.; Béchet, D.; Attaix, D.; Taillandier, D..
The 26 S proteasome is implicated in the control of many major biological functions but a reliable method for the identification of its major substrates, i.e. polyubiquitin (Ub) conjugates, is still lacking. Based on the steps present in cells, i.e. recognition and deubiquitination, we developed an affinity matrix-based purification of polyUb conjugates suitable for any biological sample. Ub-conjugates were first purified from proteasome inhibitor-treated C2C12 cells using the Ub binding domains of the S5a proteasome subunit bound to an affinity matrix and then deubiquitinated by the catalytic domain of the USP2 enzyme. This two step purification of proteasome substrates involving both protein-protein interactions and enzyme-mediated release allowed highly...
Tipo: Journal Article Palavras-chave: PROTEASOME; SUBSTRAT; UBIQUITINE; PURIFICATION.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20093f253b78&uri=/notices/prodinra1/2010/11/
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A new RNA picorna-like virus in the cotton pink bollworm Pectinophora gossypiella (Lep. : Gelechiidae) in Egypt IRD
Monsarrat, Annie; Abol-Ela, S.; Abdel-Hamid, I.; Fédière, Gilles; Kuhl, G.; El Husseini, M.; Giannotti, J..
Un nouveau virus infectant le ver rose du cotonnier, #Pectinophora gossypiella$, a été isolé et purifié à partir de larves mortes récoltées en champ de coton. Les virions purifiés de 27 plus ou moins 1 nm de diamètre, ont une forme icosaédrique et contiennent un génome à ARN. Trois protéines capsidaires de 31,7, 32,6 et 47,4 Kd ont été trouvées dans les virions. Le virus purifié n'est pas hautement infectieux pour les larves, cependant la durée du stade chrysalide est supérieure chez les lots infectés artificiellement. Dans les tissus atteints, les virions sont dispersés dans le cytoplasme des cellules de l'intestin ou bien groupés en amas paracristallins de même type que chez d'autres insectes infectés avec des picornavirus. Les adultes malades...
Tipo: Text Palavras-chave: INSECTE NUISIBLE; COTON; VIRUS; PURIFICATION; ARN; PROTEINE; ULTRASTRUCTURE; POUVOIR PATHOGENE; PARASITOIDE.
Ano: 1995 URL: http://www.documentation.ird.fr/hor/fdi:010006113
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A rapid and efficient method for the isolation of restrictable total DNA from plants of the genus Abelmoschus IRD
Kochko, Alexandre de; Hamon, Serge.
Une méthode rapide, simple et efficace a été mise au point pour extraire de l'ADN de Gombo digérable par des enzymes de restriction. Le principal obstacle était l'extrême viscosité de la solution obtenue après broyage. Ce problème a été levé en utilisant des cotylédons issus de semis faits à l'obscurité. (Résumé d'auteur)
Tipo: Text Palavras-chave: METHODOLOGIE; EXTRACTION; PURIFICATION; ELECTROPHORESE; ADN TOTAL; ENZYME DE RESTRICTION.
Ano: 1990 URL: http://www.documentation.ird.fr/hor/fdi:31799
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A strain of guinea grass mosaic virus from pearl millet in the Ivory Coast IRD
Kukla, B.; Thouvenel, Jean-Claude; Fauquet, Claude.
Tipo: Text Palavras-chave: MIL; MALADIE DES PLANTES; PATHOLOGIE VEGETALE; VIRUS; IDENTIFICATION; PURIFICATION; ANALYSE; SEROLOGIE; PROPRIETE BIOLOGIQUE; PROPRIETE PHYSICOCHIMIQUE; SYMPTOME; CULTIVAR; TRANSMISSION; VECTEUR; ETUDE EXPERIMENTALE.
Ano: 1984 URL: http://www.documentation.ird.fr/hor/fdi:010023318
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A virus cansing ringspot of Passiflora edulis in the Ivory coast IRD
De Wijs, Jean-Jacques.
Tipo: Text Palavras-chave: PATHOLOGIE VEGETALE; VIRUS; ISOLEMENT; SYMPTOME; PLANTE HOTE; INSECTE; TRANSMISSION; PURIFICATION; PASSIFLORA EDULIS; APHIS.
Ano: 1974 URL: http://www.documentation.ird.fr/hor/fdi:06973
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African cassava mosaic virus (ACMV) : stability of purified virus and improved conditions for its detection in cassava leaves by ELISA IRD
Kounounguissa, B.R.; Givord, Louise; Walter, B..
Tipo: Text Palavras-chave: PHYTOVIRUS; PURIFICATION; PARTICULE; SEROLOGIE; ANTICORPS; TEST ELISA; MANIOC.
Ano: 1989 URL: http://www.documentation.ird.fr/hor/fdi:010024389
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Amylolytic enzymes from Aspergillus hennebergi (A. niger group) : purification and characterization of amylases from solid and liquid cultures IRD
Alazard, Didier; Baldensperger, Jacques.
Tipo: Text Palavras-chave: FERMENTATION; CHAMPIGNON; ENZYME; PURIFICATION; IDENTIFICATION; ACTIVITE ENZYMATIQUE; ETUDE EXPERIMENTALE; MILIEU SOLIDE; MILIEU LIQUIDE; ASPERGILLUS HENNEBERGI; GLUCOAMYLASE.
Ano: 1982 URL: http://www.documentation.ird.fr/hor/fdi:02184
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Association d'un nouveau virus en bâtonnet avec la maladie de la nécrose à rayures du riz, Côte d'Ivoire IRD
Fauquet, Claude.
Tipo: Text Palavras-chave: PATHOLOGIE VEGETALE; RIZ; NECROSE; PURIFICATION; AGENT PATHOGENE; TRANSMISSION; EXPERIMENTATION.
Ano: 1983 URL: http://www.documentation.ird.fr/hor/fdi:04010
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Biological control of a Limacodid oil palm pest in Ivory Coast, by use of a small isometric virus IRD
Fédière, Gilles; Monsarrat, Pierre; Philippe, R..
Tipo: Text Palavras-chave: ENTOMOLOGIE APPLIQUEE; INSECTE NUISIBLE; LARVE; LUTTE BIOLOGIQUE; VIRUS; PURIFICATION; IDENTIFICATION; POUVOIR PATHOGENE; EXPERIMENTATION; LIMACODIDAE; LATOIA VIRIDISSIMA; ELAEIS GUINEENSIS.
Ano: 1984 URL: http://www.documentation.ird.fr/hor/fdi:17158
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Biological control of the oil palm pest Latoia viridissima (Lepidoptera, Limacodidae), in Côte d'Ivoire, by a new picornavirus IRD
Fédière, Gilles; Philippe, R.; Veyrunes, J.C.; Monsarrat, Pierre.
Tipo: Text Palavras-chave: ENTOMOLOGIE APPLIQUEE; LUTTE BIOLOGIQUE; INSECTE NUISIBLE; PLANTATION INDUSTRIELLE; PALMIER A HUILE; VIRUS ENTOMOPATHOGENE; PURIFICATION; POUVOIR PATHOGENE; METHODE DE LUTTE; DYNAMIQUE DE POPULATION; ETUDE COMPARATIVE; PICORNAVIRUS NOUVEAU; VIRUS A ARN.
Ano: 1990 URL: http://www.documentation.ird.fr/hor/fdi:34644
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Characterization of cowpea mottle virus on cowpea (Vigna unguiculata) in the Ivory Coast and the identification of a new vector IRD
Thouvenel, Jean-Claude; Tia, E.; Fishpool, L.D.C..
Tipo: Text Palavras-chave: LEGUMINEUSE A GRAINS; MALADIE DES PLANTES; PATHOLOGIE VEGETALE; VIRUS; PURIFICATION; PROPRIETE BIOLOGIQUE; PROPRIETE PHYSICOCHIMIQUE; SYMPTOME; PLANTE HOTE; TRANSMISSION; VECTEUR; PERTE DE RECOLTE.
Ano: 1990 URL: http://www.documentation.ird.fr/hor/fdi:010023326
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Considérations sur les propriétés immunologiques des enzymes de parasites (en particulier Trypanosoma cruzi) et sur leurs applications possibles IRD
Tibayrenc, Michel.
Tipo: Text Palavras-chave: BIOCHIMIE; IMMUNOLOGIE; ENZYME; PURIFICATION; ANTIGENE; TRYPANOSOMA CRUZI.
Ano: 1982 URL: http://www.documentation.ird.fr/hor/fdi:03278
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Contribution à l'étude du genre Isolona Englet (Annonacées) : Isolona hexaloba Engl., Isolona pilosa Diels, Isolona zenkeri Engl., Isolona campanulata Engl. et Diels IRD
Cabalion, Pierre.
Tipo: Text Palavras-chave: ALCALOIDE; CHIMIOTAXONOMIE; BOTANIQUE; PURIFICATION; ISOLEMENT; ISOLONA HEXOLOBA; ISOLONA PILOSA; ISOLONA ZENKERI; ISOLONA CAMPANULATA; TERPENE.
Ano: 1977 URL: http://www.documentation.ird.fr/hor/fdi:02689
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Control of the pink borer, Sesamia cretica Led. (Lepidoptera : Noctuidae) in maize field using a granulosis virus in Egypt IRD
Moyal, Pascal; Fédière, Gilles; Semeada, A.M.; El-Sheikh, M.A.K.; El-Sherif, S.; Abol-Ela, S..
The pink borer, #Sesamia cretica$ Lederer (#Lepidoptera$ : #Noctuidae$), is a main pest of maize in Egypt. Attacks by #S. cretica$ are usually high on early plantations of April, when the first generation of adults emerge after a period of larval hibernation. The present study aimed to evaluate the feasibility of the granulosis virus of #S. cretica$, recently isolated by Fediere et al. (1993) as a microbiological agent. A field experiment was conducted in 1997 at Sakha Agricultural Research Station, Kafr El-Sheikh governorate, North of the Nile delta, where heavy natural infestation by #S. cretica$ is of frequent occurrence on early plantations. The experimental design was randomized blocks with seven replications and five treatments. Treatments were...
Tipo: Text Palavras-chave: INSECTE NUISIBLE; LUTTE BIOLOGIQUE; VIRUS; PURIFICATION; INFESTATION; ETUDE EXPERIMENTALE.
Ano: 1997 URL: http://www.documentation.ird.fr/hor/fdi:010016041
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Détermination des traces de pyréthrinoïdes (bioperméthrine et décaméthrine) dans les substrats biologiques par chromatographie en phase gazeuse IRD
Pansu, Marc; Dhouibi, M.H.; Pinta, Maurice.
Tipo: Text Palavras-chave: INSECTICIDE; DOSAGE; METHODE D'ANALYSE; EXTRACTION; PURIFICATION; CHROMATOGRAPHIE EN PHASE GAZEUSE; PYRETHRINOIDE.
Ano: 1981 URL: http://www.documentation.ird.fr/hor/fdi:010007364
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Etiologie de la mosaïque africaine du manioc (CMD) au Nigéria IRD
Rossel, H.W.; Thottappilly, G.; Van Lent, J.M.W.; Huttinga, H..
Tipo: Text Palavras-chave: PHYTOVIRUS; ETIOLOGIE; RECHERCHE SCIENTIFIQUE; INOCULATION; ESSAI; PURIFICATION; MICROSCOPIE ELECTRONIQUE; SEROLOGIE; METHODE DE LUTTE; MOSAIQUE AFRICAINE DU MANIOC.
Ano: 1988 URL: http://www.documentation.ird.fr/hor/fdi:25567
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Etude biochimique de la nitrogénase d'Azospirillum brasilense IRD
Sanogho, S.T..
Tipo: Text Palavras-chave: NITROGENASE; ACTIVITE ENZYMATIQUE; EVALUATION; PURIFICATION; BACTERIE FIXATRICE D'AZOTE; AZOSPIRILLUM BRASILENSE.
Ano: 1982 URL: http://www.documentation.ird.fr/hor/fdi:03185
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Etude d'un virus bacilliforme du manioc : compte-rendu du travail effectué au Département de Virologie du Scottish Crop Research Institute du 11 janvier au 11 novembre 1988 IRD
Fargette, Denis.
Tipo: Text Palavras-chave: MANIOC; VIRUS; PURIFICATION; PROPRIETE BIOLOGIQUE; PROPRIETE CHIMIQUE; PROPRIETE PHYSIQUE; SEROLOGIE.
Ano: 1988 URL: http://www.documentation.ird.fr/hor/fdi:010022742
Registros recuperados: 74
Primeira ... 1234 ... Última
 

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