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Registros recuperados: 32
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Challenges in the development of drugs for the treatment of tuberculosis BJID
Shehzad,Adeeb; Rehman,Gauhar; Ul-Islam,Mazhar; Khattak,Waleed Ahmad; Lee,Young Sup.
Tuberculosis infection is a serious human health threat and the early 21st century has seen a remarkable increase in global tuberculosis activity. The pathogen responsible for tuberculosis is Mycobacterium tuberculosis, which adopts diverse strategies in order to survive in a variety of host lesions. These survival mechanisms make the pathogen resistant to currently available drugs, a major contributing factor in the failure to control the spread of tuberculosis. Multiple drugs are available for clinical use and several potential compounds are being screened, synthesized, or evaluated in preclinical or clinical studies. Lasting and effective achievements in the development of anti-tuberculosis drugs will depend largely on the proper understanding of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibiotics; Drug resistance; Tuberculosis; Pathogen.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000100012
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Characterization of Vibrio isolated from Crassostrea gigas spat suffering summer mortality outbreaks (slides) ArchiMer
Gay, Melanie; Renault, Tristan; Le Roux, Frédérique.
The pathogens related to summer mortality outbreaks are a herpes virus and two bacterial strains one belonging to Vibrio splendidus biovar II and the other to Vibrio splendidus spp. However, the feature pathogen/opportunist of these strains is still unknown. Several strains belonging to the genus Vibrio have been identified as pathogen for different mollusk species. In the context of the French program Morest, experiments of cohabitation have been used to demonstrate the potential presence of a transmissible infectious agent in batches of oysters suffering from summer mortality outbreaks. More than one hundred Vibrio strains have been isolated from these experiments. These strains have been phenotypically and genotypically characterized. Their virulence...
Tipo: Text Palavras-chave: Vibrio splendidus; Vibrio lentus; Crassostrea gigas; Summer; Spat; Pathogen; Disease transmission; Oyster culture; Mortality; Microbial contamination; Oysters.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-3343.pdf
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Comparison of Heamograms from resistant and susceptible European flat oysters, Ostrea edulis, exposed to the parasite, Bonamia ostreae ArchiMer
Cochennec, Nathalie; Renault, Tristan; Chollet, Bruno; Maffart, Patricia.
To study the cellular defence mechanisms involved in the resistance of the flat oysters against Bonamia ostreae, a comparaison of haemograms was performed between susceptible and resistant animals
Tipo: Text Palavras-chave: Pathogen; Bonamia ostreae; Ostrea edulis.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/1995/acte-2906.pdf
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Composting sewage sludge with green waste from tree pruning Scientia Agricola
Moretti,Sarah Mello Leite; Bertoncini,Edna Ivani; Abreu-Junior,Cassio Hamilton.
Sewage sludge (SS) has been widely used as organic fertilizer. However, its continuous use can cause imbalances in soil fertility as well as soil-water-plant system contamination. The study aimed to evaluate possible improvements in the chemical and microbiological characteristics of domestic SS, with low heavy metal contents and pathogens, through the composting process. Two composting piles were set up, based on an initial C/N ratio of 30:1, with successive layers of tree pruning waste and SS. The aeration of piles was performed by mechanical turnover when the temperature rose above 65 ºC. The piles were irrigated when the water content was less than 50 %. Composting was conducted for 120 days. Temperature, moisture content, pH, electrical conductivity...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biosolid; Compost; Pathogen; Soil fertility.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000500432
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Determining the minimum ripening time of artisanal Minas cheese, a traditional Brazilian cheese BJM
Martins,José M.; Galinari,Éder; Pimentel-Filho,Natan J.; Ribeiro Jr,José I.; Furtado,Mauro M.; Ferreira,Célia L.L.F..
Physical, physicochemical, and microbiological changes were monitored in 256 samples of artisanal Minas cheese from eight producers from Serro region (Minas Gerais, Brazil) for 64 days of ripening to determine the minimum ripening time for the cheese to reach the safe microbiological limits established by Brazilian legislation. The cheeses were produced between dry season (April–September) and rainy season (October–March); 128 cheeses were ripened at room temperature (25 ± 4 °C), and 128 were ripened under refrigeration (8 ± 1 °C), as a control. No Listeria monocytogenes was found, but one cheese under refrigeration had Salmonella at first 15 days of ripening. However, after 22 days, the pathogen was not detected. Seventeen days was the minimum ripening...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ripening; Artisanal Minas cheese; Serro; Pathogen; Lactic acid bacteria.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100219
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Disturbance induced decoupling between host genetics and composition of the associated microbiome ArchiMer
Wegner, Karl Mathias; Volkenborn, Nils; Peter, Hannes; Eiler, Alexander.
Background Studies of oyster microbiomes have revealed that a limited number of microbes, including pathogens, can dominate microbial communities in host tissues such as gills and gut. Much of the bacterial diversity however remains underexplored and unexplained, although environmental conditions and host genetics have been implicated. We used 454 next generation 16S rRNA amplicon sequencing of individually tagged PCR reactions to explore the diversity of bacterial communities in gill tissue of the invasive Pacific oyster Crassostrea gigas stemming from genetically differentiated beds under ambient outdoor conditions and after a multifaceted disturbance treatment imposing stress on the host. Results While the gill associated microbial communities in...
Tipo: Text Palavras-chave: Microbiota; Population structure; Stress; Pathogen; Biological invasion; Pacific oyster; Crassostrea gigas; Vibrio; Mycoplasma.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00171/28202/26430.pdf
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Economic Evaluation of Action Plans for Pathogen Control in Almonds AgEcon
Salin, Victoria; Jones, Eluned; Williams, Gary W..
The Almond Board of California proposed to require that all almonds shipped from California handlers be treated to achieve a 4-log reduction in the pathogen that leads to salmonellosis. The Assurance program is expected to add several million dollars to transactions costs in the almond marketing system. There is a wide range in these cost estimates, mainly because of the difficulty in predicting how many firms will be interested in applying for the program.
Tipo: Report Palavras-chave: Almond; Pathogen; Pathogen Control; Crop Production/Industries; Food Consumption/Nutrition/Food Safety.
Ano: 2007 URL: http://purl.umn.edu/90499
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Ecophysiological study on the Pacific oyster Crassostrea gigas naturally infected by a Chlamydia-like microorganism : effect of infection level and diet on oyster physiological responses ArchiMer
Soletchnik, Patrick; Goulletquer, Philippe; Cochennec, Nathalie; Renault, Tristan; Geairon, Philippe.
A population of Pacific ovsters, Crassostrea gigas, infected with a Chlamydia-like procaryote was reared to compare infection trends under three different feeding conditions: a starved condition, an intermediate condition with a diet of Skeletonema costatllm, and a mixed algal diet. Several physiological and pathological criteriJ: presence of gill abnormality; microscopic examination, basophilic intracytoplasmic inclusions, Feulgen reaction; condition index, filtration, food consumptiQn; fecal production and respiration were used to compare the various modalities one month after the initiation of the experiment. The Irodality of starved oysters showed reduce physiological performances in terms of scope for growth and condition index compared to oysters...
Tipo: Text Palavras-chave: Pathogen; Chlamydia; Procaryote; Crassostrea gigas; Oyster; Ecophysiology; Pathogène; Chlamydia; Procaryote; Crassostrea gigas; Huître; Ecophysiologie.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/1998/publication-2741.pdf
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Genetic improvement for disease resistance in oysters: a review ArchiMer
Degremont, Lionel; Garcia, Celine; Allen, Standish K., Jr..
Oyster species suffer from numerous disease outbreaks, often causing high mortality. Because the environment cannot be controlled, genetic improvement for disease resistance to pathogens is an attractive option to reduce their impact on oyster production. We review the literature on selective breeding programs for disease resistance in oyster species, and the impact of triploidy on such resistance. Significant response to selection to improve disease resistance was observed in all studies after two to four generations of selection for Haplosporidium nelsoni and Roseovarius crassostrea in Crassostrea virginica, OsHV-1 in Crassostrea gigas, and Martelia sydneyi in Saccostrea glomerata. Clearly, resistance in these cases was heritable, but most of the studies...
Tipo: Text Palavras-chave: Disease resistance; Oyster; Selection; Pathogen; Genetic; Mortality.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00269/38049/36139.pdf
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Genotypic profile of Listeria monocytogenes isolated in refrigerated chickens in southern Rio Grande do Sul, Brazil Ciência Rural
Mendonça,Karla Sequeira; Michael,Geovana Brenner; Nalério,Élen Silveira; Mendonça,Marcelo; Cardoso,Marisa Ribeiro Itapema; Silva,Wladimir Padilha da.
ABSTRACT: Listeria monocytogenes is of notable concern to the food industry, due to its ubiquitous nature and ability to grow in adverse conditions. This study aimed to determine the genotypic profile of L. monocytogenes strains isolated from refrigerated chickens marketed in the southern part of Rio Grande do Sul, Brazil. The strains of L. monocytogenes isolated were characterized by serotyping and Pulsed Field Gel Electrophoresis (PFGE). Three different serotypes (1/2a, 1/2b and 4e) were evaluated by PFGE, and the macrorestriction patterns utilizing enzymes AscI and ApaI, revealed five different pulsotypes. The presence of such varied genotypic profiles demonstrates the prevalence of L. monocytogenes contamination of chicken processing environments,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Contamination; Pathogen; Prevalence; Serotyping.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000100132
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Glabralysins, Potential New β-Pore-Forming Toxin Family Members from the Schistosomiasis Vector Snail Biomphalaria glabrata ArchiMer
Lassalle, Damien; Tetreau, Guillaume; Pinaud, Silvain; Galinier, Richard; Crickmore, Neil; Gourbal, Benjamain; Duval, David.
Biomphalaria glabrata is a freshwater Planorbidae snail. In its environment, this mollusk faces numerous microorganisms or pathogens, and has developed sophisticated innate immune mechanisms to survive. The mechanisms of recognition are quite well understood in Biomphalaria glabrata, but immune effectors have been seldom described. In this study, we analyzed a new family of potential immune effectors and characterized five new genes that were named Glabralysins. The five Glabralysin genes showed different genomic structures and the high degree of amino acid identity between the Glabralysins, and the presence of the conserved ETX/MTX2 domain, support the hypothesis that they are pore-forming toxins. In addition, tertiary structure prediction confirms that...
Tipo: Text Palavras-chave: Pore-forming toxin; Cry toxin; Invertebrate; Biomphalaria glabrata; Host; Pathogen; Vector snail; Innate immunity.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00601/71267/69655.pdf
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Heat shock protein 90 of Bonamia ostreae: characterization and possible correlation with infection of the flat oyster, Ostrea edulis ArchiMer
Prado Alvarez, Maria; Chollet, Bruno; Couraleau, Yann; Morga, Benjamin; Arzul, Isabelle.
In this study we described the cytosolic HSP90 of Bonamia ostreae, an intracellular parasite of Ostrea edulis haemocytes. The complete open reading frame was assembled by Rapid Amplification cDNA Ends reactions on cDNA of B. ostreae infected haemocytes. HSP90 amplification was corroborated in infected oysters and B. ostreae purified cells. The functionality of the HSP90, studied by inhibitory assays with radicicol, suggests that this protein may play a role in haemocyte invasion. Our results inform the molecular basis that governs B. ostreae-O. edulis interactions.
Tipo: Text Palavras-chave: Bivalve haemocytes; Gene; Haplosporidia; Pathogen; Protist; Radicicol.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00137/24799/22878.pdf
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Infection of clams by Perkinsus olseni as an example of the new French Surveillance Programme ArchiMer
Miossec, Laurence; Garcia, Celine; Arzul, Isabelle; Francois, Cyrille; Joly, Jean-pierre; Chollet, Bruno; Robert, Maeva; De Blas, Isabelle.
The French network for the surveillance and monitoring of mollusc health (REPAMO) was officially set up in 1992 to control the shellfish status along the French coasts according to the European directives 91/67/EEC and 95/70/EC. The objectives are 1) the surveillance of notifiable diseases present in France (Bonamiosis and Marteiliosis of flat oysters Ostrea edulis), 2) the surveillance of health status of cultured and wild populations of molluscs, 3) the study of abnormal mortality and 4) the control of exchanges from European and third countries. In this context, a new surveillance programme, called Active Targeted Survey, part of the REPAMO, was applied in 2004 in order to improve the monitoring of the health status of the French mollusc populations...
Tipo: Text Palavras-chave: Surveillance; Bivalvia; Clams; Perkinsus olseni; Pathogen; Pathology.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-6376.pdf
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Infectivity of housefly, Musca domestica (Diptera: Muscidae) to different entomopathogenic fungi BJM
Farooq,Muzammil; Freed,Shoaib.
Abstract The housefly Musca domestica is a worldwide insect pest that acts as a vector for many pathogenic diseases in both people and animals. The present study was conducted to evaluate the virulence of different local isolates of Beauveria bassiana, Metarhizium anisopliae and Isaria fumosorosea on M. domestica using two bioassay techniques: (1) adult immersion and (2) a bait method applied to both larvae and adults. The results showed evidence of a broad range of responses by both stages (larvae and adults) to the tested isolates of B. bassiana, M. anisopliae and I. fumosorosea. These responses were concentration-dependent, with mortality percentages ranging from 53.00% to 96.00%. Because it resulted in lower LC50 values and a shorter lethal time, B....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Entomopathogenic fungi; Fecundity; Housefly; Infectivity; Pathogen; Vector.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400807
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Juglanconis gen. nov. on Juglandaceae, and the new family Juglanconidaceae (Diaporthales) Naturalis
Voglmayr, H.; Castlebury, L.A.; Jaklitsch, W.M..
Molecular phylogenetic analyses of ITS-LSU rDNA sequence data demonstrate that Melanconis species occurring on Juglandaceae are phylogenetically distinct from Melanconis s.str., and therefore the new genus Juglanconis is described. Morphologically, the genus Juglanconis differs from Melanconis by light to dark brown conidia with irregular verrucae on the inner surface of the conidial wall, while in Melanconis s.str. they are smooth. Juglanconis forms a separate clade not affiliated with a described family of Diaporthales, and the family Juglanconidaceae is introduced to accommodate it. Data of macro- and microscopic morphology and phylogenetic multilocus analyses of partial nuSSU-ITS-LSU rDNA, cal, his, ms204, rpb1, rpb2, tef1 and tub2 sequences revealed...
Tipo: Article / Letter to the editor Palavras-chave: Ascomycota; Diaporthales; Molecular phylogeny; New species; Pathogen; Systematics.
Ano: 2017 URL: http://www.repository.naturalis.nl/record/622492
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Methodology for sporulation and production of monoconidial isolates of Sphaeropsis sapinea PFB - Pesquisa Florestal Brasileira
Basilio, Paula Rachel Rabelo Corrêa; Auer, Celso Garcia; Santos, Álvaro Figueredo dos; Higa, Antonio Rioyei.
Sphaeropsis sapinea is one of main pathogens that causes losses in commercial plantations of Pinusspp. Studies for control based on genetic resistance can be done by inoculating different monosporic isolates onpine populations and selectioning the resistant individuals. This work describes a methodology for pycnidia production in vitro and S. sapinea monoconidial cultures, using an agar-water medium with sterile pine needles. Four S. sapinea isolates were tested e pycnidia and mature conidia were observed after 14 days of incubation, under continuous fluorescent light, in BOD, at 25 °C. Pycnidia were triturated in sterile water and conidia germinated after two hours of incubation in water-agar, in BOD, at 25 °C. Germinated conidia formed colonies which...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Inoculum; Pathogen; Pine. Inóculo; Patógeno; Pínus..
Ano: 2010 URL: http://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/139
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Molecular and phenotypic characterization of Vibrio aestuarianus subsp francensis subsp nov., a pathogen of the oyster Crassostrea gigas ArchiMer
Garnier, Matthieu; Labreuche, Yannick; Nicolas, Jean-louis.
Eleven Vibrio isolates invading the hemolymph of live and moribund oysters (Crassostrea gigas) collected in the field and from a hatchery in France, were characterized by a polyphasic approach. Phylogenetic analysis of 16S rRNA, gyrB and toxR genes indicated high homogeneity between these strains and the Vibrio aestuarianus type strain (ATCC35048(T)), and confirmed previous 16S rRNA analysis. In contrast, DNA:DNA hybridization was from 61% to 100%, while phenotypic characters and virulence tests showed a large diversity between the strains. Nevertheless, several common characters allowed the isolates to be distinguished from the reference strain. On the basis of several distinct phenotypic characteristics, it is proposed to establish two subspecies within...
Tipo: Text Palavras-chave: Polyphasic approach; Summer mortalities; Crassostrea gigas; Pathogen; French isolates; Vibrio aestuarianus.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4961.pdf
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Morphological and molecular characterization of Curvularia lunata pathogenic to andropogon grass Bragantia
Santos,Patrícia Resplandes Rocha dos; Leão,Evelynne Urzêdo; Aguiar,Raimundo Wagner de Souza; Melo,Maruzanete Pereira de; Santos,Gil Rodrigues dos.
ABSTRACT The fungal genus Curvularia is associated with a number of diseases in plants, commonly producing foliar spots in forage grasses. The objective of this study was to characterize the morphological and molecular diversity of the isolates of Curvularia sp. associated with Andropogon seeds, and to assess both their capacity to transmit disease and the pathogenicity of this fungus to crop. Ten isolates of Curvularia sp. were sourced from Andropogon seeds from agricultural producing regions in the Brazilian states Tocantins and Pará. Morphological characterization was achieved by observing fungus colonies and conidia and molecular characterization by DNA extraction and amplification with sequence-specific primers. The disease transmission was evaluated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Andropogon L.; Curvularia lunata; Diagnosis; Phylogeny; Pathogen.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052018000200326
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Norwalk virus-specific binding to oyster digestive tissues ArchiMer
Le Guyader, Soizick; Loisy, Fabienne; Atmar, R; Hutson, A; Estes, M; Ruvoen-clouet, N; Pommepuy, Monique; Le Pendu, J.
The primary pathogens related to shellfishborne gastroenteritis outbreaks are noroviruses. These viruses show persistence in oysters, which suggests an active mechanism of virus concentration. We investigated whether Norwalk virus or viruslike particles bind specifically to oyster tissues after bioaccumulation or addition to tissue sections. Since noroviruses attach to carbohydrates of the histo-blood group family, tests using immunohistochemical analysis were performed to evaluate specific binding of virus or viruslike particles to oyster tissues through these ligands. Viral particles bind specifically to digestive ducts (midgut, main and secondary ducts, and tubules) by carbohydrate structures with a terminal N-acetylgalactosamine residue in an alpha...
Tipo: Text Palavras-chave: Bioaccumulation; Shellfish; Escherichia coli; Pathogen; Oyster digestive tissue; Noroviruses.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1542.pdf
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Pacem in terris pathogenibus bonae voluntatis: molluscs-pathogens relationships prospects ArchiMer
Berthe, Franck.
In 1999, the world aquaculture production of molluscs was estimated at 9.4 million tonnes which represents approximately a 23% contribution to the global aquaculture production. Aquaculture of molluscs has been growing at an average annual rate of 11% over the past decades, although some activities - such as aquaculture of pectens or abalones - were growing even more rapidly. In many countries, there is a significant mollusc aquaculture, traditionally based on wild stocks. However, these natural populations frequently do not fulfil market demand, because of low value of the product, over-fishing of the resource and/or mass mortality outbreaks caused by diseases. A frequent answer to this situation has been the introduction of new stocks or new species in...
Tipo: Text Palavras-chave: Mortality; Mollucs; Pathogen.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/publication-2780.pdf
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