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Registros recuperados: 7
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Dry fruit as source of fungal contaminants or functional compounds? Ciênc. Tecnol. Aliment.
HARTWIG,Naralice; FERREIRA,Cláudia Fetter Jorge; COLAZZO,Carolina Carvalho; KUPSKI,Larine; BADIALE-FURLONG,Eliana.
Abstract This study evaluated the role of dry fruits as a source of fungal contaminants or functional compounds. Dry apple, apricot, candied fruits, prune, and white and black raisin were evaluated regarding patulin and ergosterol content, centesimal composition, pH, acidity, pectin, and phenolic profile. The ergosterol was detected in 38% of the samples and patulin (PAT) in 89%, the apricot samples stood out most by contaminant level. Apricot, candied fruit, and prunes presented moisture values beyond the other dry fruits. The pH ranged from 3.8 to 4.6; acidity from 0.1 to 1.0%; sugar content from 1.7 to 5.5%; and levels of soluble and insoluble pectin from 1.6 to 5.1% and 10.9% to 27.3%, respectively. The apples showed a higher content of free phenolic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ergosterol; Patulin; Pearson correlation; Phenolic acids; Pectin.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000100076
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Intralaboratory optimization and validation of a method for patulin determination in grapes by Thin-Layer Chromatography BJM
Oliveira,Renata Cabrera de; Bando,Érika; Machinski Junior,Miguel.
The aim of this work was to optimize and validate, by intralaboratorial procedures, a method for the determination of patulin in grapes by thin-layer chromatography. The steps of extraction, cleanup, detection and quantification were optimized. For the validation of the method, recovery assays with standard solutions and artificially contaminated samples were carried out. The mean recovery and the variation coefficient were 65.4% and 7.58%, respectively. The optimized conditions were: 50 mL of grape juice, three extraction stages (with 100 mL of ethyl acetate in the first stage and 50 mL in second and third stages), and 100 µL of ethanol to solubilize the extract. The solvent-system used was toluene - ethyl acetate - formic acid (6:3:1), and 0.5% MBTH in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Patulin; Mycotoxins; Grape; Thin-layer chromatography.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200023
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Patulin accumulation in apples during storage by Penicillium expansum and Penicillium griseofulvum strains BJM
Welke,Juliane Elisa; Hoeltz,Michele; Dottori,Horacio Alberto; Noll,Isa Beatriz.
A part of apples destined to juice production is generally of poor quality. Apples from cold storage or recently harvest (ground harvested or low quality apples) are stored under ambient conditions until they are processed. Since Penicillium expansum and P. griseofulvum are the principal fungal species isolated from stored apples in Brazil, the objective of this study was to investigate the ability of these strains to produce patulin in apples and report the consequences of this type of storage in loss of quality. The toxin was quantified using thin layer chromatography and charge-coupled device camera (TLC-CCD). The rate and quantities that P. expansum and P. griseofulvum can grow and produce patulin are highly dependent on the fungal strain and time....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Patulin; Apple; Penicillium expansum; Penicillium griseofulvum.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100021
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Penicillium expansum versus antagonist yeasts and patulin degradation in vitro BABT
Coelho,Alexandre Rodrigo; Celli,Marcos Giovani; Ono,Elisabete Yurie Sataque; Wosiacki,Gilvan; Hoffmann,Fernando Leite; Pagnocca,Fernando Carlos; Hirooka,Elisa Yoko.
Taking into account the preliminary antagonistic/biodegradation property showed by Pichia membranifaciens and Sporobolomyces roseus, which decreased the initial patulin concentration of 588.4 to 290.0 µg/mL, ability of P. ohmeri 158 in biocontrol against Penicillium expansum and patulin decrease in vitro was performed. The culture supernatant of P. ohmeri 158 was effective against 66.17% micelial growth, indicating antibiosis related with the killer phenomenon. The initial patulin concentration of 223 µg in the presence of P. ohmeri 158 cells was decreased over 83% of the original concentration, when incubated at 25ºC/2 days and > 99% after 5 days incubation time, with undetectable patulin level after 15 days. The initial pH 4.0 decreased to pH 3.3...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Penicillium expansum; Antagonist yeasts; Biocontrol; Patulin; Degradation.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400019
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Polyphasic taxonomy of the heat resistant ascomycete genus Byssochlamys and its Paecilomyces anamorphs Naturalis
Samson, R.A.; Houbraken, J.; Varga, J.; Frisvad, J.C..
Byssochlamys and related Paecilomyces strains are often heat resistant and may produce mycotoxins in contaminated pasteurised foodstuffs. A comparative study of all Byssochlamys species was carried out using a polyphasic approach to find characters that differentiate species and to establish accurate data on potential mycotoxin production by each species. Phylogenetic analysis of the ITS region, parts of the â-tubulin and calmodulin genes, macro- and micromorphological examinations and analysis of extrolite profiles were applied. Phylogenetic analyses revealed that the genus Byssochlamys includes nine species, five of which form a teleomorph, i.e. B. fulva, B. lagunculariae, B. nivea, B. spectabilis and B. zollerniae, while four are asexual, namely P....
Tipo: Article / Letter to the editor Palavras-chave: Byssochlamys; Emodin; Eurotiales; Extrolites; Heat resistance; Mycophenolic acid; Paecilomyces; Patulin.
Ano: 2009 URL: http://www.repository.naturalis.nl/record/532420
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The application of PCR in the detection of mycotoxigenic fungi in foods BJM
Konietzny,Ursula; Greiner,Ralf.
It is estimated that 25 to 50% of the crops harvested worldwide are contaminated with mycotoxins. Because of the toxic and carcinogenic potential of mycotoxins, there is an urgent need to develop detection methods that are rapid and highly specific. The highly advanced physico-chemical methods for the analysis of mycotoxins in use, have the disadvantage that highly sophisticated clean-up and/or derivatization procedures must be applied. An alternative could be the detection of the mycotoxigenic moulds themselves, especially as molecular techniques have been introduced recently as powerful tools for detecting and identifying fungi. PCR methods for the detection of aflatoxigenic Aspergilli, patulin-producing Penicillum and trichothecene- as well as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Fumonisin; Patulin; Polymerase chain reaction; Trichothecene.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400001
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The development of an analytical method for two mycotoxins, patulin and verruculogen, and survey of their presence in commercial tomato pulp BJM
Kawashima,Luciane M.; Soares,Lucia M. Valente; Massaguer,Pilar R. de.
The mycotoxin patulin causes gastroinstestinal distress, neurotoxic and immunotoxic effects in animals. It can be produced by several species of Penicillium, Aspergillus and Byssochlamys and it has been found in fruits, vegetables and cereals. Verruculogen is a toxin produced mainly by Penicillium and Aspergillus spp. and causes severe tremors in affected animals. Tomatoes are especially susceptible to fungi invasion and their products need to be investigated for possible mycotoxin contamination. A method for the determination of patulin and verruculogen in tomato products was developed involving an extraction with ethyl acetate, a cleanup by silica gel column and determination and confirmation by high performance liquid chromatography with diode array...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycotoxins; Patulin; Verruculogen; Tomato products.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300017
Registros recuperados: 7
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