Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 10
Primeira ... 1 ... Última
Imagem não selecionada

Imprime registro no formato completo
Activation of Prophenoloxidase and Removal of Bacillus subtilis from the Hemolymph of Acheta domesticus (L.) (Orthoptera: Gryllidae) Neotropical Entomology
SILVA,CLEONOR C.A. DA.
Insect phenoloxidase is considered an important mediator in defense reaction against pathogens and parasites. It is present in the hemolymph of most insects as an inactive proenzyme, called prophenoloxidase. In Acheta domesticus (L.) the phenoloxidase activity was studied in the presence of Bacillus subtilis. The bacteria induced the activation of the prophenoloxidase and increased the level of phenoloxidase activity when injected into the hemocoel of A. domesticus nymphs. The level of increase was comparable to those observed in nymphs injected with laminarin, an activator of the proenzyme. Incubating B. subtilis in serum with phenoloxidase activated in vitro and then injecting into the insects accelerated the removal of the bacteria from the hemolymph in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hemolynph; Phenoloxidase; Hemocyte; Defense reaction.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2002000300024
Imagem não selecionada

Imprime registro no formato completo
Characterization of abalone Haliotis tuberculata-Vibrio harveyi interactions in gill primary cultures ArchiMer
Pichon, Delphine; Cudennec, Benoit; Huchette, Sylvain; Djediat, Chakib; Renault, Tristan; Paillard, Christine; Auzoux-bordenave, Stephanie.
The decline of European abalone Haliotis tuberculata populations has been associated with various pathogens including bacteria of the genus Vibrio. Following the summer mortality outbreaks reported in France between 1998 and 2000, Vibrio harveyi strains were isolated from moribund abalones, allowing in vivo and in vitro studies on the interactions between abalone H. tuberculata and V. harveyi. This work reports the development of primary cell cultures from abalone gill tissue, a target tissue for bacterial colonisation, and their use for in vitro study of host cell—V. harveyi interactions. Gill cells originated from four-day-old explant primary cultures were successfully sub-cultured in multi-well plates and maintained in vitro for up to 24 days....
Tipo: Text Palavras-chave: Haliotis tuberculata; Vibrio harveyi; Gill cell culture; Pathogenicity; Phenoloxidase; Phagocytosis.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00146/25683/23745.pdf
Imagem não selecionada

Imprime registro no formato completo
Copper improves the production of laccase by the white-rot fungus Pleurotus pulmonarius in solid state fermentation BABT
Tychanowicz,Giovana Kirst; Souza,Daniela F. de; Souza,Cristina G. M.; Kadowaki,Marina Kimiko; Peralta,Rosane Marina.
Pleurotus pulmonarius (Fr) Quélet, produced laccase as the main ligninolytic enzyme when cultivated on solid-state cultures using corn cob as substrate. The addition of copper greatly increased the production of enzyme. The addition of 25.0 mM CuSO4 increased the level of laccase from 270 to 1,420 U.L-1 and the fungus showed high resistance to copper under the conditions used in this work.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Copper; Laccase; Pleurotus pulmonarius; Phenoloxidase; Solid state fermentation.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000600002
Imagem não selecionada

Imprime registro no formato completo
Demonstration of a true phenoloxidase activity and activation of a ProPO cascade in Pacific oyster, Crassostrea gigas (Thunberg) in vitro ArchiMer
Hellio, Claire; Bado Nilles, Anne; Gagnaire, Beatrice; Renault, Tristan; Thomas-guyon, Helene.
The prophenoloxidase (ProPO) system is the origin of melanin production and is considered to be an innate defence mechanism in invertebrates. In different bivalve species, phenoloxidase (PO) is present in the haemolymph as an inactive form of ProPO. The present study focuses on the Pacific adult oyster, Crassostrea gigas, an economically important bivalve species along French coasts. The results indicate that many factors may inhibit the PO-like activity. These include: phenylthiourea (PTU), sodium diethylthiocarbamate (DETC), beta-mercaptoethanol and tropolone, which repressed the spontaneous PO activity. The activation of PO-like activity in C. gigas acellular fraction by lipopolysaccharide (LPS) involved participation of other factors, including at...
Tipo: Text Palavras-chave: Prophenoloxidase; Phenoloxidase; Oyster; Immunity; Defence; Crassostrea gigas.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2926.pdf
Imagem não selecionada

Imprime registro no formato completo
Effect of unilateral and bilateral eyestalk ablation in Litopenaeus vannamei male and female on several metabolic and immunologic variables ArchiMer
Sainz Hernandez, J; Racotta, Ilie; Dumas, S; Hernandez Lopez, J.
Eyestalk ablation is the most common procedure to induce gonadic maturation in commercial hatcheries of penaeid shrimp. In addition to reproduction, other physiological and metabolic processes are affected by removal of the X-organ sinus gland complex located in the eyestalk. In this study, the effect of unilateral and bilateral eyestalk ablation on the concentration of several hemolymph metabolites and phenoloxidase system in female and male shrimp was investigated. As a consequence of reducing or suppressing molt-inhibiting hormone (MIH) production, the duration of the molting cycle was significantly shorter in eyestalk-ablated shrimp: bilaterally (10 days), unilaterally (17 days), and shrimp that were not ablated (24 days). Mortality was significantly...
Tipo: Text Palavras-chave: Sinus gland; Shrimp; Phenoloxidase; Hemolymph metabolites.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-6408.pdf
Imagem não selecionada

Imprime registro no formato completo
Effects of cadmium on aneuploidy and hemocyte parameters in the Pacific oyster, Crassostrea gigas ArchiMer
Bouilly, Karine; Gagnaire, Beatrice; Bonnard, Marc; Thomas-guyon, Helene; Renault, Tristan; Miramand, Pierre; Lapegue, Sylvie.
Pacific oysters, Crassostrea gigas, are commonly reared in estuaries where they are exposed to anthropogenic pollution. Much research has been made on the toxicity of cadmium to aquatic organisms because the compound recurrently contaminates their environment. Our study examined the influence of cadmium on aneuploidy level (lowered chromosome number in a percentage of somatic cells) and hemocyte parameters in C. gigas at different stages of life. Adults and juveniles were exposed to two different concentrations of cadmium. The first concentration applied was equivalent to a peak value found in Marennes-Oleron bay (Charente-Maritime, France; 50 ng L-1) and the second was 10 times higher (500 ng L-1). Exposure to 50 ng L-1 cadmium caused a significant...
Tipo: Text Palavras-chave: Phenoloxidase; Flow cytometry; Hemocytes; Aneuploidy; Crassostrea gigas; Cadmium.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1687.pdf
Imagem não selecionada

Imprime registro no formato completo
Immune responses of phenoloxidase and superoxide dismutase in the manila clam Venerupis philippinarum challenged with Vibrio tapetis – Part I: Spatio-temporal evolution of enzymes’ activities post-infection ArchiMer
Le Bris, Cedric; Richard, Gaelle; Paillard, Christine; Lambert, Christophe; Seguineau, Catherine; Gauthier, Olivier; Pernet, Fabrice; Guerard, Fabienne.
Manila clams, Venerupis philippinarum (Adams and Reeve, 1850), were experimentally challenged with two Vibrio tapetis strains: CECT4600T, the causative agent of Brown Ring Disease (BRD); and LP2 supposedly non-pathogenic in V. philippinarum. Changes in phenoloxidase (PO) and superoxide dismutase (SOD), two major enzymes involved in immunity, were studied in two tissues, the mantle and hemolymph for 30 days after infection in the extrapallial cavity. Bacterial infection in V. philippinarum resulted in modulation of PO and SOD activities that was both tissue- and time-dependent. A response at early times was detected in the mantle and was associated with significant increases in PO and SOD activities in LP2- and CECT4600T-challenged clams 36h post injection....
Tipo: Text Palavras-chave: Venerupis philippinarum; Vibrio tapetis; Immunity; Phenoloxidase; Superoxide dismutase.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00221/33257/31733.pdf
Imagem não selecionada

Imprime registro no formato completo
In vitro effects of cadmium and mercury on Pacific oyster, Crassostrea gigas (Thunberg), haemocytes ArchiMer
Gagnaire, Beatrice; Thomas Guyon, H; Renault, Tristan.
In the past decades. shellfish culture has developed in a significant way around the world. However, culture areas are Often Subject to recurring anthropic pollution. The recrudescent presence of industrial wastes is a source of heavy metals and results ill pollutant transfer towards the aquatic environment in estuarine areas. Because of their mode of life. bivalves. including Mussels and oysters. are suggested as ideal indicator organisms. The development of techniques allowing the analysis of the effects of Pollutants on bivalve biology may lead to the monitoring of pollutant transfer in estuarine areas. in this context. the effects of cadmium and mercury On defence mechanisms were analysed in Pacific oysters. Crassostrea gigas. Pollutant effects were...
Tipo: Text Palavras-chave: Toxicity; Cellular activities; Phenoloxidase; Mercury; Cadmium; Flow cytometry; Haemocytes; Crassostrea gigas.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-691.pdf
Imagem não selecionada

Imprime registro no formato completo
Phenoloxidase activity and haemolymph cytology in honeybees challenged with a virus suspension (deformed wings virus DWV) or phosphate buffered suspension (PBS) Ciência Rural
Millanta,Francesca; Sagona,Simona; Mazzei,Maurizio; Forzan,Mario; Poli,Alessandro; Felicioli,Antonio.
ABSTRACT: The innate immune system of honeybees mainly consists in antimicrobial peptides, cellular immunity and melanisation. In order to investigate the immune response of honeybees to immune stressors, three stress degrees were tested. Newly emerged bees naturally DWV-infected were collected from a Varroa mite-free apiary and divided into three experimental groups: naturally DWV infected bees, PBS injected bees, and artificially DWV super infected bees. Phenoloxidase activity and haemolymph cellular subtype count were investigated. Phenoloxidase activity was highest (P<0.05) in DWV-superinfected bees, and the haemocyte population differed within the three observed groups. Although, immune responses following DWV infection have still not been...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cellular immunity; Phenoloxidase; Honeybees; DWV; Haemocytes.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000200550
Imagem não selecionada

Imprime registro no formato completo
SAR of Sponge-Inspired Hemibastadin Congeners Inhibiting Blue Mussel PhenolOxidase ArchiMer
Niemann, Hendrik; Hagenow, Jens; Chung, Mi-young; Hellio, Claire; Weber, Horst; Proksch, Peter.
Hemibastadin derivatives, including the synthetically-derived 5,5-dibromohemibastadin-1 (DBHB), are potent inhibitors of blue mussel phenoloxidase (PO), which is a key enzyme involved in the firm attachment of this invertebrate to substrates and, thus, a promising molecular target for anti-fouling research. For a systematic investigation of the enzyme inhibitory activity of hemibastadin derivatives, we have synthesized nine new congeners, which feature structural variations of the DBHB core structure. These structural modifications include, e.g., different halogen substituents present at the aromatic rings, different amine moieties linked to the (E)-2-(hydroxyimino)-3-(4-hydroxyphenyl)propionic acid, the presence of free vs. substituted aromatic hydroxyl...
Tipo: Text Palavras-chave: Antifouling; Hemibastadin; Phenoloxidase; Sponges; Copper; Mytilus edulis.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00273/38432/73167.pdf
Registros recuperados: 10
Primeira ... 1 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional