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Isolation and purification of bromelain from waste peel of pineapple for therapeutic application BABT
Bresolin,Iara Rocha Antunes Pereira; Bresolin,Igor Tadeu Lazzarotto; Silveira,Edgar; Tambourgi,Elias Basile; Mazzola,Priscila Gava.
The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, maintaining its enzymatic activity. The batch adsorption of bromelain was evaluated in terms of total protein and enzymatic activity using Langmuir and Langmuir-Freundlich models. Results showed that the process could be suitable for the recovery and purification of the enzyme, maintaining its specific activity.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bromelain; Pineapple peel; Downstream processing; Waste; Therapeutic applications; Enzymatic activity.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600012
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การประเมินคุณค่าทางโภชนะและคาร์โบไฮเดรตในรูปน้ำตาลที่ละลายได้ของเศษเหลือจากสับปะรด Thai Agricultural
Anan Chaokaur; Yaniga Laikhonburi; Chotirod Kunmee; Chawanrat Santhong; Supawadee Chimthong.
The objective of this study was to determine nutritive value and sugar soluble carbohydrate ofpineapple residue. Six treatments were (1) fresh pineapple peel, (2) composted pineapple peel, (3) grind composted pineapple peel, (4) pineapple pulpmeal, (5) pineapple leaf, and (6) pineapple crown. The experiment was conducted in completely randomized design. The results were shown that structural carbohydrate was higher in pineapple peel than pineapple pulp, crown and leaf. Fat content was highest in pineapple leaf. Soluble sugar carbohydrate was higher in pineapple leaf and pulp. In conclusion, pineapple leaf and pulp have potential of being roughage source for dairy and beef cattle.
Tipo: PhysicalObject Palavras-chave: Pineapple peel; Pineapple residue; Animal feeding; Cattle; Nutritive value; Carbohydrate sugar; สับปะรด; เศษเหลือจากสับปะรด; อาหารสัตว์; อาหารหยาบ; โคนม; โคเนื้อ; คุณค่าอาหารสัตว์; การวิเคราะห์องค์ประกอบทางเคมี; ปริมาณคาร์โบไฮเดรต; ค่าน้ำตาล; ค่าไขมัน.
Ano: 2014 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5749
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การศึกษาการผลิตโปรตีนเซลล์เดียวจากเปลือกสับปะรด โดยใช้ยีสต์และบาซิลัสซัปติลิสเพื่อพัฒนาเป็นอาหารสัตว์ Thai Agricultural
Manat-Sanun Nopparatmaitree; Pornpan Seanphoom; Warangkana Kitpipit; Krittiya Lertchunhakiat.
This experiment was conducted to study on single cell protein production from pineapple peel by Sacharomyces cerevisiae and Bacillus subtilis as animal feedstuff. The experimental design was 4 x 3 factorial in CRD experiment with two factors. The factor A was comprised fermented period (0, 15, 30 and 45 day). While, the factor B was comprised of type of microorganism (Sacharomyces cerevisiae, Bacillus subtilis and Sacharomyces cerevisiae combined with Bacillus subtilis). The results showed that interaction between factor A and Factor B on incubation pH 3.88-4.16, temperature 28.62-29.78 C and yield 11.22-16.94 % were highly significantly (P<0.01). For nutrients composition, the group was fermented Sacharomyces cerevisiae combined with Bacillus subtilis...
Tipo: PhysicalObject Palavras-chave: Pineapple peel; Saccharomyces cerevisiae; Bacillus subtilis; Feedstuff; Single cell protein; Crude protein; Digestible protein; Pepsin enzyme; เปลือกสับปะรด; ยีสต์; บาซิลัส ซัสติลิส; การหมัก; การผลิตอาหารสัตว์; โปรตีนเซลล์เดียว; ค่าโปรตีน; คุณค่าทางโภชนะ; การย่อยโปรตีน; เอนไซม์เปปซิน.
Ano: 2013 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5803
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