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Registros recuperados: 8
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Ativação de defesa em cacaueiro contra a murcha-de-verticílio por extratos naturais e acibenzolar-S-metil. Repositório Alice
PEREIRA, R.B.; RESENDE, M.L.V.; RIBEIRO JÚNIOR, P.M.; AMARAL, D.R.; LUCAS, G.C.; CAVALCANTI, F.R..
O objetivo deste trabalho foi avaliar o potencial de extratos fúngicos e vegetais na redução da murcha-de-verticílio do cacaueiro, as atividades da peroxidase e polifenoloxidase e o conteúdo de lignina. Mudas de cacaueiro foram pulverizadas com filtrado de micélio de Rhizopus sp. (FMR), quitosana de Rhizopus sp. (QMR) e Trichoderma sp. (QMT), extratos de casca in natura e seca de maracujá, extrato metanólico de casca seca de frutos de maracujá (MMS) e acibenzolar-S-metil (ASM ? 0.2 mg mL-1) e sete dias depois, submetidas à inoculação de Verticillium dahliae. O ASM reduziu a murcha-de-verticílio em 38,0%, seguido dos extratos FMR, QMT, MMS e QMR, que apresentaram reduções em 22,8, 20,1, 19,2 e 15,7%, respectivamente, em relação à testemunha. Plantas...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Theobroma cacao; Verticillium dahliae; Lignina; Polifenoloxidase; Lignin; Peroxidase; Polyphenoloxidase.
Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/handle/doc/126296
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Effects of oligosaccharides from endophytic Fusarium oxysporum Dzf17 on activities of defense-related enzymes in Dioscorea zingiberensis suspension cell and seedling cultures Electron. J. Biotechnol.
Li,Peiqin; Luo,Haiyu; Meng,Jiajia; Sun,Weibo; Wang,Xiaohan; Lu,Shiqiong; Peng,Youliang; Zhou,Ligang.
Background Three oligosaccharides (EOS, WOS and SOS) were respectively prepared from the corresponding polysaccharides, namely exopolysaccharide (EPS), water-extracted mycelial polysaccharide (WPS) and sodium hydroxide-extracted mycelial polysaccharides (SPS) from the endophytic fungus Fusarium oxysporum Dzf17. In this study, the effects of EOS, WOS and SOS on the activities of the defense-related enzymes, namely phenylalanine ammonia lyase (PAL), polyphenoloxidase (PPO) and peroxidase (POD) in its host plant Dioscorea zingiberensis cultures were investigated. Results For the suspension cell cultures of D. zingiberensis, the highest PAL activity was induced by 0.5 mg/mL of WOS at 48 h after treatment, which was 4.55-fold as that of control. Both PPO and...
Tipo: Journal article Palavras-chave: Endophytic fungus; Phenylalanine ammonia lyase; Peroxidase; Polyphenoloxidase; Polysaccharide.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000400003
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Hydrothermal treatment minimizes the effects of refrigeration in okra fruits Horticultura Brasileira
Ribeiro,Fernanda Cristina S; Silva,Tania P; Neves,Ludmila LM; Finger,Fernando Luiz.
ABSTRACT This study evaluated the involvement of peroxidase and polyphenoloxidase on the enzymatic browning of okra and the role of catalase in the appearance of chilling injury in cultivars Amarelinho and Mammoth Spineless treated with hot water. Okra fruits from two cultivars were immersed in water at 40°C, during 15 and 30 minutes. The control treatment was not immersed, and all fruits were stored at 5°C. Visual analysis of fruits was carried out and total chlorophyll content and total phenolic compounds were determined. The activity of the enzymes catalase, peroxidase and polyphenoloxidase was analyzed. In all treatments, the cultivar Mammoth Spineless showed higher values of chlorophyll content and soluble phenolic compounds compared to cultivar...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abelmoschus esculentus; Catalase; Peroxidase; Polyphenoloxidase; Heat treatment.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362017000400499
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Parâmetros relacionados às alterações físicas, químicas e bioquímicas em pera da cultivar Willians, maçã da cultivar Fuji. Repositório Alice
SOUZA, J. M. L. de; FREIRE, V. A. P.; RUTZ, D.; RUIZ, W. A.; TORALLES, R. P.; ELIAS, M. C..
Estudou-se os parâmetros que caracterizam os principais processos bioquímicos de transformação de substratos no período pós-colheita de frutas. Como matéria-prima utilizou-se maçãs (Mallus communis) da cultivar Fuji e pera (Pyrus communis L.) da cultivar Willians, obtidos no comércio de Pelotas, RS. Analisou-se acidez total, sólidos solúveis, açúcares redutores, fenóis totais, atividades de PPO e POD. Confirmou-se a influência do pH, da temperatura e do ácido ascórbico na atividade das enzimas PPO e POD, as quais demosntraram especificidade para os substratos catecol 1% (p/v) e para guaiacol, 0,4% (p/v), respectivamente.
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Calidad de la fruta; Pérdidas postcosecha; Manzanas; Polyphenoloxidase; Catecol oxidasa; Peroxidasa; Fruta; Qualidade; Pós-Colheita; Maçã; Malus Communis; Pêra; Pyrus Communis; Polifenoloxidase; Peroxidase; Fruit quality; Postharvest losses; Apples; Pears; Catechol oxidase.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1104481
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Peroxidase (POD) and polyphenoloxidase (PPO) in grape (Vitis vinifera L.) Ciência e Agrotecnologia
Troiani,Estela de Pieri; Tropiani,Clariza Tomé; Clemente,Edmar.
The enzimatic activity of peroxidase (POD) and polyphenoloxidase (PPO) extracted from three grape cultivars (Vitis vinifera L.), cultivated in Marialva city, state of Paraná, was evaluated in this study. The enzymatic extracts were prepared starting from the Rubi, Borbon and Benitaka grape cultivars pulp and peel. The activity of the peroxidase was 53.00 units/100 g in the extract from the Rubi cultivar peel, and 327.00 units/100 g from the Benitaka cultivar, these values being superior to those observed in the same cultivars pulp extracts, which were 7.67 units/100 g and 44.00 units/100 g respectively. However, the result was opposite in the Borbon cultivar, with values of 141.11 units/100 g in the pulp and 11.50 units/100 g in the peel being found. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Peroxidase; Polyphenoloxidase; Activity; Grape; Vitis vinifera.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542003000300019
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Polyphenoloxidase activity in peaches after vibration REA
Dantas,Tiago B. H.; Ferraz,Antônio C. de O.; Honório,Sylvio L.; Lima,Giuseppina P. P..
The vibration during transportation is one of the main causes of fruit damage. The actual methodologies used for damage evaluation in fruits seem to be subjective, since most of them are based on visual evaluation. In this study the effect of vibration, for one and two hours, on polyphenoloxidase (PPO) activity in peach pulp was investigated. The relation among PPO activity variation, postharvest time and resting time were also considered. Results showed that vibration affects PPO activity. However, it was not possible to correlate PPO activity variation and vibration.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polyphenoloxidase; Vibration; Damage; Peach.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162013000200010
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Respiratory activity and browning of minimally processed sweet potatoes Horticultura Brasileira
Moretti,Celso L.; Araújo,Alessandra L.; Marouelli,Waldir A.; Silva,Washington L.C..
Sweet potatoes (Ipomoea batatas L.), 'Brazlândia Roxa', 'Brazlândia Branca' and 'Princesa' were harvested at optimum maturity to evaluate respiratory activity and browning susceptibility of minimally processed roots. After harvest, non-blemished roots were graded for size (18±2cm) and diameter (5±1cm), and minimally processed inside a cold room. Processed roots were placed in sealed glass jars and stored at 3±0.5°C to evaluate respiratory activity during a 4-hour period or were packed in plastic films with partial vacuum to evaluate development of browning. Packages were stored under refrigerated conditions (3±0.5°C) for 5 days. Daily, minimally processed roots were evaluated for browning according to a scale ranging from 0 (extremely browned) to 5 (no...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ipomoea batatas; Carbon dioxide; Mechanical damage; Polyphenoloxidase; Postharvest; Sensory analysis; Storage.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362002000300020
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Some Biochemical properties of polyphenoloxidase from spearmint (Mentha arvensis) BABT
Neves,Valdir Augusto; Picchi,Douglas Gatte; Silva,Maraiza Aparecida da.
Polyphenoloxidase (PPO; EC 1.14.18.1) extracted from Mentha arvensis leaves was isolated by (NH4)2SO4 precipitation and extensive dialysis. Its optimum pH and temperature varied with the substrate. The PPO showed activity with various diphenols. Km values were found 0.825, 0.928 and 7.41mM for caffeic acid, 4-methylcatechol and catechol, respectively. On heat-inactivation, half of the activity was lost after 60 and 15 sec at 70 and 75ºC, respectively. Measuring of residual activity showed a stabilizing effect of sucrose at various temperatures with activation energy (Ea) for inactivation increasing with sucrose concentration from 0 to 40% (w/w). Ea values of 78.13; 80.37; 82.79 and 81.00 kJ/Mol were found for 0, 15; 30 and 40% sucrose, respectively. PPO...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polyphenoloxidase; Mentha arvensis; Characterization; Heat inactivation; Inhibitors.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400025
Registros recuperados: 8
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