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Registros recuperados: 39
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A complementary method for production of tetraploid Crassostrea gigas using crosses between diploids and tetraploids with cytochalasin B treatments ArchiMer
Mccombie, Helen; Ledu, Christophe; Phelipot, Pascal; Lapegue, Sylvie; Boudry, Pierre; Gerard, Andre.
We present a new method to produce tetraploid Crassostrea gigas by cytochalasin B inhibition of polar body 2 expulsion in diploid females crossed with tetraploid males. This offers a means of direct introgression of genetic characters from selected diploid to tetraploid lines, avoiding a triploid step. Offspring larval ploidy shifted over time and depended on size, with tetraploids more frequent among the smaller larvae and triploids among the large. Viable tetraploids were found at 4 and 6 months, indicating the technique was successful. The possibility that gynogenesis occurred was tested by microsatellite analysis to confirm the presence of paternally inherited alleles. These were present in all animals of the 2n x 4n + CB (female first) cross. However,...
Tipo: Text Palavras-chave: Oyster breeding; Gynogenesis; Polar body; Cytochalasin B; Polyploidy; Crassostrea gigas.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-632.pdf
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A karyotypic study of three southern Brazilian Asteraceae species using fluorescence in situ hybridization with a 45S rDNA probe and C-CMA3 banding Genet. Mol. Biol.
Fregonezi,Jéferson N.; Torezan,José M.D.; Vanzela,André L.L..
The Asteraceae, one of the largest families of flowering plants, contains about 1,100 genera and 20,000 species, and is well known for its extensive karyotypic variation. In this study, conventional Feulgen staining, C-CMA3 banding, and fluorescence in situ hybridization with a 45S rDNA probe were used to determine the chromosome number and the number and physical position of GC-rich heterochromatin and 45S rDNA sites in three Asteraceae weed species (Crepis japonica, Galinsoga parviflora and Chaptalia nutans). The three species exhibited karyotype differences in the chromosome number and shape, as a commom feature of Asteraceae. However, the 45S rDNA sites always occurred on the short chromosomal arms, associated with GC-heterochromatin. Althought of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CMA3; FISH; Heterochromatin; Polyploidy; 45S rDNA.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200016
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A new approach for obtaining rapid uniformity in rice (Oryza sativa L.) via a 3x x 2x cross Genet. Mol. Biol.
Xing,Shaochen; Cai,Yuhong; Zhou,Kaida.
A triploid (2n = 3x = 36) rice plant was obtained by screening a twin seedling population in which each seed germinated to two or three sprouts that were then crossed with diploid plants. One diploid plant was chosen among the various F1 progenies and developed into an F2 population via self-pollination. Compared with the control variety Shanyou 63, this F2 population had a stable agronomical performance in field trials, as confirmed by the F-test. The stability of the F2 population was further substantiated by molecular analysis with simple sequence repeat markers. Specifically, of 160 markers assayed, 37 (covering all 12 chromosomes) were polymorphic between the parental lines. Testing the F1 hybrid individually with these markers showed that each PCR...
Tipo: Info:eu-repo/semantics/article Palavras-chave: F-test; Polyploidy; Rice; SSR marker; Stability.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200020
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Arachis hoehnei, the probable B genome donor of Arachis hypogaea based on crossability, cytogenetical and molecular studies Open Agri
Mallikarjuna, N..
Palavras-chave: Genomes; Hybrids; Polyploidy; Cytogenetics; DNA; Groundnuts; Mosses; Genetic polymorphism; Polymorphism; Genetic improvement.
Ano: 2006 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3177
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Biotechnology applied to Annona species: a review Rev. Bras. Frutic.
Encina,Carlos Lopez; Martin,Elisabeth Carmona; Lopez,Antonio Arana; Padilla,Isabel Maria Gonzalez.
Annonaceae is an ancient family of plants including approximately 50 genera growing worldwide in a quite restricted area with specific agroclimatic requirements. Only few species of this family has been cultivated and exploited commercially and most of them belonging to the genus Annona such as A. muricata, A. squamosa, the hybrid A. cherimola x A. squamosa and specially Annona cherimola: the cherimoya, commercially cultivated in Spain, Chile, California, Florida, México, Australia, Ecuador, Peru, Brazil, New Zealand and several countries in South and Central America. The cherimoya shows a high degree of heterozygosis, and to obtain homogeneous and productive orchards it is necessary to avoid the propagation by seeds of this species. Additionally, the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Annona; In vitro; Micropropagation; Protoplasts; Organogenesis; Polyploidy.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452014000500002
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Chromosomal and morphological studies of diploid and polyploid cytotypes of Stevia rebaudiana (Bertoni) Bertoni (Eupatorieae, Asteraceae) Genet. Mol. Biol.
Oliveira,Vanessa M. de; Forni-Martins,Eliana R.; Magalhães,Pedro M.; Alves,Marcos N..
In this study, we examined the chromosome number and some morphological features of strains of Stevia rebaudiana. The chromosomes were analyzed during mitosis and diakinesis, and the tetrad normality and pollen viability were also assessed. In addition, stomata and pollen were measured and some plant features were studied morphometrically. All of the strains had 2n = 22, except for two, which had 2n = 33 and 2n = 44. Pairing at diakinesis was n = 11II for all of the diploid strains, whereas the triploid and tetraploid strains had n = 11III and n = 11IV, respectively. Triploid and tetraploid plants had a lower tetrad normality rate than the diploids. All of the strains had inviable pollen. Thus, the higher the ploidy number, the greater the size of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Asteraceae; Pollen; Polyploidy; Stevia rebaudiana; Stevioside; Stomata.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200015
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Chromosome characterization and variability in some Iridaceae from Northeastern Brazil Genet. Mol. Biol.
Alves,Lânia Isis F.; Lima,Saulo Antônio A.; Felix,Leonardo P..
The chromosomes of 15 species of Iridaceae of the genera Alophia, Cipura, Eleutherine, Neomarica and Trimezia (subfamily Iridoideae) were examined after conventional Giemsa staining. The karyotypes of Alophia drummondii (2n = 14+1B, 28, 42 and 56), Cipura paludosa (2n = 14), C. xanthomelas (2n = 28) and Eleutherine bulbosa (2n = 12) were asymmetric; Neomarica candida, N. caerulea, N. humilis, N. glauca, N. gracilis, N. northiana and Neomarica sp. (2n = 18); N. cf. paradoxa (2n = 28), Trimezia fosteriana (2n = 52), T. martinicensis (2n = 54) and T. connata (2n = 82) were all generally symmetric. New diploid numbers of 2n = 56 for Alophia drummondii, 2n = 18 for N. candida, N. humilis, N. glauca, and N. gracilis, 2n = 28 for N. cf. paradoxa, and 2n = 82 for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Iridaceae; Disploidy; Karyotypic evolution; Polyploidy; Asymmetrical karyotype.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200016
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Chromosome studies in Orchidaceae from Argentina Genet. Mol. Biol.
Daviña,Julio Rubén; Grabiele,Mauro; Cerutti,Juan Carlos; Hojsgaard,Diego Hernán; Almada,Rubén Dario; Insaurralde,Irma Stella; Honfi,Ana Isabel.
The center of diversity of Argentinean orchids is in the northeast region of the country. Chromosome numbers and karyotype features of 43 species belonging to 28 genera are presented here. Five chromosome records are the first ones at the genus level; these taxa are Aspidogyne kuckzinskii (2n = 42), Eurystyles actinosophila (2n = 56), Skeptrostachys paraguayensis (2n = 46), Stigmatosema polyaden (2n = 40) and Zygostates alleniana (2n = 54). In addition, a chromosome number is presented for the first time for 15 species: Corymborkis flava (2n = 56), Cyclopogon callophyllus (2n = 28), C. oliganthus (2n = 64), Cyrtopodium hatschbachii (2n = 46), C. palmifrons (2n = 46), Galeandra beyrichii (2n = 54), Habenaria bractescens (2n = 44), Oncidium edwallii (2n =...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosome number; Karyotype features; Polyploidy; Karyology; Orchids; South America.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400024
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Cytological and genome size data analyzed in a phylogenetic frame: Evolutionary implications concerning Sisyrinchium taxa (Iridaceae: Iridoideae) Genet. Mol. Biol.
Burchardt,Paula; Souza-Chies,Tatiana T.; Chauveau,Olivier; Callegari-Jacques,Sidia M.; Brisolara-Corrêa,Lauís; Inácio,Camila Dellanhese; Eggers,Lilian; Siljak-Yakovlev,Sonja; de Campos,José Marcello Salabert; Kaltchuk-Santos,Eliane.
Abstract Sisyrinchium is the largest genus of Iridaceae in the Americas and has the greatest amount of cytological data available. This study aimed at investigating how genomes evolved in this genus. Chromosome number, genome size and altitude from species of sect. Viperella were analyzed in a phylogenetic context. Meiotic and pollen analyses were performed to assess reproductive success of natural populations, especially from those polyploid taxa. Character optimizations revealed that the common ancestor of sect. Viperella was probably diploid (2n = 2x =18) with two subsequent polyplodization events. Total DNA content (2C) varied considerably across the phylogeny with larger genomes detected mainly in polyploid species. Altitude also varied across the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ancestral chromosome number; DNA content; Meiotic behavior; Pollen viability; Polyploidy.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200288
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Ecological setup, ploidy diversity, and reproductive biology of Paspalum modestum, a promising wetland forage grass from South America Genet. Mol. Biol.
Karunarathne,Piyal; Feduzka,Cristian; Diego,Hojsgaard.
Abstract With ever-rising demand for food, forage breeding for intensification of cattle production is also taking a leap. In South America, cattle production systems are displaced to marginal areas poorly exploited with cultivated pastures yet with high potential for growing stocking rates. This places the need for using native genetic resources to breed locally adapted plant genotypes that benefits from better forage quality, yield, and lesser threat to the local biodiversity. Paspalum modestum Mez is a grass species that produces quality forage and grows in marginal areas like estuaries and floodplains, suitable for introduction in breeding programs. In this study we characterize the species' reproductive biology and ecological preferences needed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apomixis; Ecological niche; Plant breeding; Polyploidy; Sexuality.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000300304
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Effects of cytochalasin B on fertilization and ploidy in the Pacific oyster Crassostrea gigas ArchiMer
Ledu, Christophe; Mccombie, Helen.
The effects of cytochalasin B on fertilization and ploidy in Crassostrea gigas were examined in a controlled experiment. This enabled us to better understand why variable ploidy results have been found with the retention of polar bodies in previous studies on bivalve mollusks, and what the interaction with fertilization may be. By looking at the early development of Crassostrea gigas embryos with sequential samples stained using Hoechst 33258, a fluorescent DNA-specific dye, we compared normal evolution of fertilized eggs with that obtained with cytochalasin B treatment. In treated eggs, embryos with both three and four pronuclei were found after the second meiotic division. When embryos had three pronuclei, these were identified as the female pronucleus...
Tipo: Text Palavras-chave: Crassostrea gigas; Fertilization; Cytochalasin B; Polar body; Polyploidy.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-2845.pdf
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Exploitation of wild relatives of pigeonpea and chickpea for resistance to Helicoverpa armigera Open Agri
Mallikarjuna, N.,( Nalini Mallikarjuna ).
Palavras-chave: Pigeonpeas; Chickpeas; Hybridization; Genes; Gene pools; Interspecific hybridization; Hybrids; Genomes; Gene transfer; Polyploidy.
Ano: 2007 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3283
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Genetic improvement and selection in shellfish : a review based on oyster research and production ArchiMer
Boudry, Pierre; Barre, Marc; Gerard, Andre.
Despite the economic importance of shellfish, genetic improvement has not yet had a great impact on these species. Shellfish farming is traditionally based on wild species whose natural populations are often overexploited and do not fulfil market demand. Up to now, the most effective answer 10 disease problems or for the improvement of productivity has been the introduction of new species. However, the introduction of new species is constrained by their ecological impact and is also limited by the availability 01 suitable species. Genetic improvement of local species should therefore be of great importance as a viable alternative for the long term sustainability 01 the shellfish Industry. The different approaches 10 genetic improvement include...
Tipo: Text Palavras-chave: Selective breeding; Hybridization; Polyploidy; Introduction; Oyter; Sélection; Hybridation; Polyploïdie; Introduction; Huîtres.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/1998/publication-2740.pdf
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Het Dryopteris affinis-complex in Nederland Naturalis
Hovenkamp, P.; Hendrikx, B.; Roskam, H.; Winter, W. de.
With the help of flow-cytometric analysis of nuclear DNA content and morphology, we establish the presence in the Netherlands of two taxa of the Dryopteris affinis complex: diploid D. affinis subsp. affinis, triploid D. affinis subsp. borreri, and the hybrids D × complexa (tetraploid) and D. × critica (pentaploid). Onderzoek aan kern-DNA gewicht en morfologie van recente vondsten van het Dryopteris affinis-complex wijst uit dat in Nederland twee taxa en twee hybrides met redelijke zekerheid zijn gevonden: dediploïde D. affinis subsp. affinis, de triploïde D. affinis subsp. borreri, en de hybriden D × complexa (tetraploïd) en D. × critica (pentaploïd). We analyseren een aantal onderscheidende kenmerken en maken de keuze om deze taxa te behandelen als...
Tipo: Article / Letter to the editor Palavras-chave: Dryopteris affinis; Soortscomplex; Species complex; Uitbreiding; Range expansion; Genoomgrootte; Genome size; Cytotypes; Polyploidie; Polyploidy; Morfometrie; Morphometrics.
Ano: 2018 URL: http://www.repository.naturalis.nl/record/669050
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Heterochromatin and numeric chromosome evolution in Bignoniaceae, with emphasis on the Neotropical clade Tabebuia alliance Genet. Mol. Biol.
Cordeiro,Joel M.P.; Kaehler,Miriam; Souza,Luiz Gustavo; Felix,Leonardo P..
Abstract Bignoniaceae is a diverse family composed of 840 species with Pantropical distribution. The chromosome number 2n = 40 is predominant in most species of the family, with n = 20 formerly being considered the haploid base number. We discuss here the haploid base number of Bignoniaceae and examine heterochromatin distributions revealed by CMA/DAPI fluorochromes in the Tabebuia alliance, as well as in some species of the Bignonieae, Tecomeae, and Jacarandeae tribes. When comparing the chromosome records and the phylogenies of Bignoniaceae it can be deduced that the base number of Bignoniaceae is probably n = 18, followed by an ascendant dysploidy (n = 18 → n = 20) in the most derived and diverse clades. The predominant heterochromatin banding patterns...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosome number; CMA/DAPI; Handroanthus; Polyploidy.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100303
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Induction and identification of polyploids in Cattleya intermedia Lindl. (orchidaceae) by in vitro techniques Ciência Rural
Silva,Paulo Artur Konzen Xavier de Mello e; Callegari-Jacques,Sídia; Bodanese-Zanettini,Maria Helena.
The effect of colchicine on the protocorm-like bodies of Cattleya intermedia L. (Orchidaceae) cultured in vitro was studied. Different concentrations of the drug (0.00, 0.05, 0.10 and 0.20%) as well as two times of culture (4 or 8 days) were tested to determine the best treatment for the induction of tetraploid plants. Of the 3 colchicine levels applied, 0.05 and 0.10% of drug treatments appeared to be effective on the production of mixoploids and tetraploids and could be further used in breeding programs. The suitability of stomatal area and stomata density as criteria for the distinction between diploids and tetraploids was also tested. The results suggested that tetraploid plants could be identified with a fair amount of certainty when the screening was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattleya; Colchicine; Chromosome number; Orchid improvement; Polyploidy; Stomata analysis.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000100017
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Influence of ploidy and metal-metal interactions on the accumulation of Ag, Cd, and Cu in oysters Crassostrea gigas thunberg ArchiMer
Amiard, Jean-claude; Perrein Ettajani, H; Gerard, Andre; Baud, Jean-pierre; Amiard Triquet, C.
The present study was designed to compare the response to contaminants in diploid with triploid specimens of the oyster Crassostrea gigas. The reproduction investment in bivalve molluscs has priority on somatic growth. Thus, genetic sterilization by triploidy induction enables the energy flux to be directed toward somatic growth and glucide storage. Bioaccumulation was examined for Ag (10 mug/L), Cd (10 mug/L), and Cu (30 mug/L) to determine if the response to metals follows similar patterns in diploid (2n) and triploid (3n) groups. The effect of ploidy was also evaluated as a function of dry weight of soft tissue and condition index. Moreover, the reciprocal influence of these metals on their incorporation was studied. The results showed that the major...
Tipo: Text Palavras-chave: Oyster culture; Metal bioaccaumulation; Polyploidy.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-791.pdf
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International Workshop on Groundnuts Open Agri
R.W.( Editor ).
Palavras-chave: Groundnuts; Root nodulation; Genotypes; Biological interaction; Mycotoxins; Chromosomes; Fungi; Breeds (animals); Polyploidy.
Ano: 1980 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3978
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Intra- and interspecific karyotypic variations of the genus Senna Mill. (Fabaceae, Caesalpinioideae) Acta Botanica
Cordeiro,Joel Maciel Pereira; Felix,Leonardo P..
ABSTRACT Although the chromosome number 2n = 28 predominates in most species of the genus Senna, variations are often observed, resulting from either polyploidy (2n = 42, 56, 112) or disploidy (2n = 22, 24, 26) events. To better understand the karyotypic variations in Senna, we examined heterochromatin patterns of 10 species of that genus using chromomycin A3 (CMA) and 4’6-diamidino-2-phenylindole (DAPI) staining, and reviewed information on the chromosome counts of 72 species of that genus. The CMA/DAPI banding patterns were relatively variable among the 10 species, both in terms of the numbers of bands (from two to 26) and their locations on the chromosomes (terminal or proximal regions). Our review indicated that 2n = 28 is the most common ploidy among...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosome number; CMA/DAPI; Disploidy; Leguminosae; Polyploidy.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000100128
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Karyological studies in Brazilian species of Lippia L. (Verbenaceae) Anais da ABC (AABC)
Sousa,Saulo M.; Torres,Giovana A.; Viccini,Lyderson F..
The genus Lippia (Verbenaceae) comprise around 160 species spread out mainly in South and Central Americas with few African species, some of them with potential medicinal use. Brazil is one the most important centers of diversity with approximately 75% of the species described so far. Innumerous species are endemic and poorly studied especially at a cytological level. Here, chromosomal length, karyomorphology and chromosome asymmetry of twelve Brazilian species of Lippia were evaluated [L. alba (Miller) N.E.Brown, L. diamantinensis Glaz., L. florida Cham., L. hermanioides Cham., L. lacunosa Mart. & Schauer, L. lupulina Cham., L. pohliana Schauer, L. pseudothea (St. Hil) Schauer, L. rosella Moldenke, L. rotundifolia Cham., L. rubella Moldenke and L....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosomes; Cytology; Dysploidy; Karyology; Lippia; Polyploidy.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000400017
Registros recuperados: 39
Primeira ... 12 ... Última
 

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