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Registros recuperados: 22
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A spleen tyrosine kinase inhibitor attenuates the proliferation and migration of vascular smooth muscle cells Biol. Res.
Seo,Hyang‑Hee; Kim,Sang Woo; Lee,Chang Youn; Lim,Kyu Hee; Lee,Jiyun; Choi,Eunhyun; Lim,Soyeon; Lee,Seahyoung; Hwang,Ki‑Chul.
Abstract Background Pathologic vascular smooth muscle cell (VSMC) proliferation and migration after vascular injury promotes the development of occlusive vascular disease. Therefore, an effective chemical agent to suppress aberrant proliferation and migration of VSMCs can be a potential therapeutic modality for occlusive vascular disease such as atherosclerosis and restenosis. To find an anti-proliferative chemical agent for VSMCs, we screened an in-house small molecule library, and the selected small molecule was further validated for its anti-proliferative effect on VSMCs using multiple approaches, such as cell proliferation assays, wound healing assays, transwell migration assays, and ex vivo aortic ring assay. Results Among 43 initially screened...
Tipo: Journal article Palavras-chave: Syk kinase inhibitor; BAY61-3606 VSMC; Proliferation; Migration.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100403
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BRD4 inhibition suppresses cell growth, migration and invasion of salivary adenoid cystic carcinoma Biol. Res.
Wang,Limei; Wu,Xiuyin; Wang,Ruolin; Yang,Chengzhe; Li,Zhi; Wang,Cunwei; Zhang,Fenghe; Yang,Pishan.
Abstract Background: Bromodomain-containing protein 4 (BRD4) inhibition is a new therapeutic strategy for many malignancies. In this study, we aimed to explore the effect of BRD4 inhibition by JQ1 on in vitro cell growth, migration and invasion of salivary adenoid cystic carcinoma (SACC). Methods: The human normal epithelial cells and SACC cells (ACC-LM and ACC-83) were treated with JQ1 at concentrations of 0, 0.1, 0.5 or 1 μM. Cell Counting Kit-8 (CCK-8) assay was performed to evaluate cell proliferation. Cell apoptosis and cell cycle distribution was evaluated by Flow cytometry. Immunofluorescence staining was used to examine the expression of BRD4 in SACC cells. The quantitative real-time polymerase chain reaction (qRT-PCR) assay and western...
Tipo: Journal article Palavras-chave: Salivary adenoid cystic carcinoma; BRD4 inhibition; Proliferation; Migration; Epithelial-mesenchymal transition.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100213
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Di and tripeptides from marine sources can target adipogenic process and contribute to decrease adipocyte number and functions ArchiMer
Ben Henda, Yesmine; Laamari, Mariem; Lanneluc, Isabelle; Travers, Marie-agnes; Agogue, Helene; Arnaudin, Ingrid; Bridiau, Nicolas; Maugard, Thierry; Piot, Jean-marie; Sannier, Frederic; Bordenave-juchereau, Stephanie.
The effect of 11 marine-derived cryptides was investigated on proliferation, differentiation and maturation of human white pre-adipocytes (HWP). They were all formerly identified as potent Angiotensin-Converting-Enzyme inhibitors.Val-Trp (VW),Val-Tyr (VY), Lys-Tyr (KY), Lys-Trp (KW), Ile-Tyr (IY), Ala-Pro (AP),Val-Ile-Tyr (VIY), Leu-Lys-Pro (LKP), Gly-Pro-Leu (GPL), Ala-Lys-Lys (AKK) and Val-Ala-Pro (VAP) were previously found in fish products and coproducts as well as other marine resources like wakame. Treatment with AP, VAP and AKK greatly affected viability of HWP during the proliferation period while KW and VW treatment reduced the number of viable cells during the differentiation stage. A GPL and IY incubation during the differentiation stage allowed...
Tipo: Text Palavras-chave: Bioactive peptides; Obesity; Human white pre-adipocytes'; Proliferation; Differentiation; Adipocyte differentiation markers.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00269/38014/36308.pdf
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Early Proliferation of Bone Marrow Mononuclear Cells on Collagen Membrane, Bone Graft and Tooth Cementum International Journal of Morphology
Milagres,Débora; Rueff-Barroso,Carlos R; Carvalho,Simone N; Carvalho,Laís de; Monte-Alto-Costa,Andréa; Fischer,Ricardo G; Porto,Luís Cristóvão.
The ultimate goal of periodontal therapy is to repair the damaged periodontal supporting tissues, permitting regeneration of the periodontal ligament. However, the cell response, the supportive matrix and the bioactive molecules use have not yet been well established. Bone marrow mononuclear cells were extracted from rat femurs and tibiae and cultured on a cross-linked collagen membrane, bone graft, or molar tooth to compare cell attachment and early proliferation on these materials. Cell attachment was quantified by light microscopy at 24, 48 and 72h, and cell proliferation was observed under a SEM after 72h. After 24h, the number of cells on bone graft was similar to that of the control and more than twice compared to collagen membrane (q=7.473...
Tipo: Journal article Palavras-chave: Stem cells; Collagen membrane; Bone graft; Cementum; Proliferation.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000400045
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Effects of c-FLIPL Knockdown in Cervical Uterine Carcinoma Cell Lines International Journal of Morphology
Ili,Carmen G; Brebi,Priscilla; Garcia,Patricia; Leal,Pamela; Lopez,Jaime; Tapia,Oscar; Letelier,Pablo; Weber,Helga; Castillo,Jonathan; Roa,Juan C.
Overexpression of Short and Raji variants of Cellular FLICE-like inhibitory protein (c-FLIP) is capable of inhibiting apoptosis, while the function of the Long isoform depends of c-FLIPL concentration in cells. The aim of this study was to determine the effects of c-FLIPL knockdown in cervical cell lines. SiHa, C-4I and C-33A cervical cancer cell lines were analyzed. c-FLIPL level expression was determined by quantitative real-time PCR and western blotting. c-FLIPL was transiently downregulated by siRNA. The effects of knockdown of c-FLIPL on cell viability, proliferation and apoptosis were assessed by comparing with scrambled siRNA-transfected cells. SiHa and C-4I c-FLIPL knockdown cells showed increased viability compared with scrambled siRNA-transfected...
Tipo: Journal article Palavras-chave: Apoptosis; Proliferation; Cervical cancer; SiRNA; C-FLIPL.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000200036
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Effects of Supplemental Boron on Intestinal Proliferation and Apoptosis in African Ostrich Chicks International Journal of Morphology
Sun,PengPeng; Luo,You; Wu,Xin Tong; Ansari,Abdur Rahman; Wang,Jing; Yang,KeLi; Xiao,Ke; Peng,KeMei.
Boron is an essential trace element which plays an important role in process of metabolism and the function of the tissues. However, the effects of boron on the intestinal cells in African ostrich chicks are poorly reported. Therefore, this study was designed to investigate the role of boron on proliferation and apoptosis of the intestinal cells. A total of 36, ten day-old ostrich chicks were randomly divided into six groups and fed on the same basal diet supplemented with 0, 40, 80, 160, 320 and 640 mg/L boric acid in drinking water for 80 days. Proliferatingcell nuclearantigen (PCNA) wasused to test the proliferation indexof intestine in different group byimmunohistochemicalstaining (IHC). Apoptoticcellsofintestinewere detectedbyDutp-biotin nick end...
Tipo: Journal article Palavras-chave: Apoptosis; Boron; Intestine; Ostrich chicks; Proliferation.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000300002
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Entry, dispersion and differentiation of microglia in the developing central nervous system Anais da ABC (AABC)
NAVASCUÉS,JULIO; CALVENTE,RUTH; MARÍN-TEVA,JOSÉ L.; CUADROS,MIGUEL A..
Microglial cells within the developing central nervous system (CNS) originate from mesodermic precursors of hematopoietic lineage that enter the nervous parenchyma from the meninges, ventricular space and/or blood stream. Once in the nervous parenchyma, microglial cells increase in number and disperse throughout the CNS; these cells finally differentiate to become fully ramified microglial cells. In this article we review present knowledge on these phases of microglial development and the factors that probably influence them.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microglia; Hematopoietic lineage; Meninges; Migration; Proliferation.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652000000100013
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Etude de la prolifération des algues vertes dans le bassin d'Arcachon ArchiMer
Manaud, Francois; Maurer, Daniele; Trut, Gilles; Auby, Isabelle.
Introduction Massive proliferations of algae and, in particular green macroalgae, have been known all over the continents for several decades. They affect various littoral sites and belong to diverse species (Ulva, Enteromorpha and especially Cladophora). In 1990, on the North Brittany shore, more than 80 000 m3 ulvas have been mechanically removed by affected communities. Up until recently, the Arcachon basin escaped from such phenomenon, even if the diversity and quantity of algae found there had already undergone increasing periods in the past. However, the first algae proliferations only started in the 80's, which scale was large enough to be a nuisance to all activities taking place in the basin. At first (starting in1982), those proliferations were...
Tipo: Text Palavras-chave: Basin of Arcachon; Green seaweed; Proliferation; Bassin d'Arcachon; Algue verte; Proliferation.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/1994/rapport-1653.pdf
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Galectin-8 promotes migration and proliferation and prevents apoptosis in U87 glioblastoma cells Biol. Res.
Metz,Claudia; Döger,Remziye; Riquelme,Elizabeth; Cortés,Priscilla; Holmes,Christopher; Shaughnessy,Ronan; Oyanadel,Claudia; Grabowski,Catalina; González,Alfonso; Soza,Andrea.
BACKGROUND: Glioblastoma is one of the most aggressive cancers of the brain. Malignant traits of glioblastoma cells include elevated migration, proliferation and survival capabilities. Galectins are unconventionally secreted glycan-binding proteins that modulate processes of cell adhesion, migration, proliferation and apoptosis by interacting with beta-galactosides of cell surface glycoproteins and the extracellular matrix. Galectin-8 is one of the galectins highly expressed in glioblastoma cells. It has a unique selectivity for terminally sialylated glycans recently found enhanced in these highly malignant cells. A previous study in glioblastoma cell lines reported that Gal-8 coating a plastic surface stimulates two-dimensional motility. Because in other...
Tipo: Journal article Palavras-chave: Galectin-8; Glioblastoma; Cancer; Cell cycle; Apoptosis; Proliferation; Migration.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100033
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Inventaire des ulves en Bretagne. Année 1997. Rapport de synthese ArchiMer
Merceron, Michel.
The yearly study of Ulva in Brittany aims at building a time series of its proliferations and corresponding nutrient loadings. The goal is to detect results of wilful loading reductions, and to distinguish them from natural variations. This first actually quantified study is the starting bench-mark of the process. Forty-five concerned sites were listed in 1997. They are nearly the same as in 1988, 1991 and 1994 (previous rough census). The Côtes d'Armor and then the Finistère are the most affected departments. The Morbihan is less and file et Vilaine not at all. Ulva biomass was precisely quantified in about fifteen locations during the growing season, along with nitrogen loadings of local streams (mostly nitrate). The fluxes of nitrogen - the limiting...
Tipo: Text Palavras-chave: Bretagne; Nitrate; Prolifération; Ulve; Eutrophisation; Brittany; Nitrate; Proliferation; Ulva; Eutrophication.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/1998/rapport-1580.pdf
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Inventaire des Ulves en Bretagne année 1998 - rapport de synthèse ArchiMer
Merceron, Michel.
The 1998 survey of green tides on the Brittany coast was performed in the same way as that of 1997 in order to allow comparison. 11 includes an inventory ofail the concerned sites, and a precise assessment ofthirteen ofthem, the most loaded. On these ones, nitrogen fluxes were surveyed during the critical period, and the green seaweed stock on the beach and in the first waves was quantified. The climatic conditions were opposite between the two years, very dry in spring and summer 1997, and wet in 1998. The irradiance contrast was inverse, and less pronounced. The nitrate contents and mostly the flows were more important in 1998. They generated nitrogen fluxes about 3.3 times those of 1997. The total number of sites concerned in 1998 is clearly higher than...
Tipo: Text Palavras-chave: Bretagne; Nitrate; Prolifération; Ulve; Eutrophisation; Brittany; Nitrate; Proliferation; Ulva; Eutrophication.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/rapport-2665.pdf
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Knockdown of ubiquitin-specific peptidase 39 inhibited the growth of osteosarcoma cells and induced apoptosis in vitro Biol. Res.
Gan,Zhihua; Han,Kun; Lin,Shuchen; Hu,Haiyan; Shen,Zan; Min,Daliu.
Abstract Background Ubiquitin specific peptidase 39 (USP39), an essential factor in the assembly of the mature spliceosome complex, has an aberrant expression in several cancer. However, its function and the corresponding mechanism on human osteosarcoma has not been fully explored yet. Methods The mRNA and DNA copies of USP39 were increased in osteosarcoma cancer tissues compared with the one in human normal tissues according to datasets from the publicly available Oncomine database. A further western blot analysis also demonstrated an aberrant endogenous expression of USP39 in three different osteosarcoma cells. Then lentivirus-mediated short hairpin RNA (shRNA) was designed to silence USP39 in human osteosarcoma cell line U2OS, which is used to test...
Tipo: Journal article Palavras-chave: Cell cycle; Migration; Osteosarcoma; Proliferation; USP39.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100210
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Malathion Affects Spermatogenic Proliferation in Mouse International Journal of Morphology
Penna-Videau,S; Bustos-Obregón,E; Cermeño-Vivas,J. R; Chirino,D.
The restriction of the mechanisms of cell proliferation in murine seminiferous epithelium, in terms of induction of programmed cell death until recently has not been fully analyzed. The aim of this work was to assess the effect of Malathion (MP) on testicular morphology and function in mouse spermatogenesis. For the experiments, male albino mice of strain NMRI-IVIC, weighing between 30-40 g were used, and divided into control and experimental groups of 5 each. The animals of the experimental groups were injected with a single dose of MP: 241mg/kg weight (1/12 LD 50 ) resuspended in 0.9% saline, intraperitoneally. Animals were sacrificed at 8.3, 16.6 and 33.2 days post-injection (first, second and third spermatogenic cycles). Testicular samples were...
Tipo: Journal article Palavras-chave: Malathion; Testis; Mouse; Apoptosis; Morphology; P53; Proliferation.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400023
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Marées vertes en Bretagne : état actuel des connaissances ArchiMer
Merceron, Michel.
On tens of sandy beaches of Brittany, a green seaweed (Ulva) blooms during spring and summer. In the sites where the proliferation is the most important, the total biomass amounted to 20 000 t of wet weight in 1997, at the time of maximum. These "green tides" occur every year in nearly the same locations. They have a very harmful impact on local tourism. In many communes, beached and rotting algae are collected and dumped (43 000 m 3 as a whole in 1997). The main development of biomass takes place between the surf zone and the bare sand.The outbreak requires three coexisting conditions : 1) a sandy beach gently sloping, 2) a weak residual current (i.e. once the tidal back and forth movement has been filtered), 3) a nitrogen loading, even moderate (ammonium...
Tipo: Text Palavras-chave: Brittany; Eutrophication; Proliferation; Ulva; Marine algae; Bretagne; Eutrophisation; Prolifération; Ulves; Algues marines.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/acte-46.pdf
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MICE TESTICULAR DAMAGE ELICITED BY MALATHION International Journal of Morphology
Bustos-Obregón,Eduardo; González-Hormazábal,Patricio.
Organophosphorous pesticides are a healh problem worldwide, mostly for agroworkers, which are around 2600 millions of person in the year 2001. Little is known about male reproductive damage elicited by these chemicals. The acute effects of malathion (single intraperitoneal injection), 1/12 the LD50 to mice were analyzed at 1, 8, 16, 35 and 40 days after injection, in terms of spermatogenic cell proliferation and apoptosis and of Sertoli cell compromise as revealed by immunocytochemical detection of CK-18 (cytokeratin). The micronuclei test was done to assess for genotoxic activity of the agropesticide. The results showed decreased germ cell number followed by compensatory spermatogonial proliferation by 16 days (as shown by thymidine-H3 testicular uptake),...
Tipo: Journal article Palavras-chave: Spermatogenesis; Organophosphorous agropesticides; Proliferation; Apoptosis.
Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000200010
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Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279 Agronomy
Kavand, Somayeh; Agricultural Biotechnology Research Institute of Iran; Kermani, Maryam Jafarkhani; Agricultural Biotechnology Research Institute of Iran; Haghnazari, Ali; Agricultural Biotechnology Research Institute of Iran; Khosravi, Pegah; Agricultural Biotechnology Research Institute of Iran; Azimi, Mohamad Reza; Agricultural Biotechnology Research Institute of Iran.
In Iran, a large number of Rosa species have been exposed to extinction and therefore preservation techniques are necessary to safeguard their future. In the present investigation, the objectives were to optimize the micropropagation and medium-term conservation of one of these wild species, Rosa pulverulenta. At proliferation stage, the maximum number of new leaves (9.6) were produced on the medium containing 4 µM BAP + 0 µM GA3, whereas the maximum number of axillary shoots (4.1) were observed in the medium containing 4 µM BAP + 3 µM GA3. The results for rooting experiments suggested that the highest increase in stem height (49.5 mm) at the acclimatization stage was observed in plantlets treated with 1 µM...
Tipo: Laboratory analysis Palavras-chave: In vitro conservation; Micropropagation; Proliferation; Recovery; Rooting; Rosa pulverulenta in vitro conservation; Micropropagation; Proliferation; Recovery; Rooting; Rosa pulverulenta.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10279
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MicroRNA-98 inhibits the cell proliferation of human hypertrophic scar fibroblasts via targeting Col1A1 Biol. Res.
Bi,Sheng; Chai,Linlin; Yuan,Xi; Cao,Chuan; Li,Shirong.
Abstract Background Hypertrophic scarring (HS) is a severe disease, and results from unusual wound healing. Col1A1 could promote the hypertrophic scar formation, and the expression of Col1A1 in HS tissue was markedly higher than that in the normal. In present study, we aimed to identify miRNAs as post-transcriptional regulators of Col1A1 in HS. Methods MicroRNA-98 was selected as the key miRNA comprised in HS. The mRNA levels of miR-98 in HS tissues and the matched normal skin tissues were determined by qRT-PCR. MTT and flow cytometry were used to determine the influence of miR-98 on cell proliferation and apoptosis of HSFBs, respectively. Col1A1 was found to be the target gene of miR-98 using luciferase reporter assay. Luciferase assay was performed...
Tipo: Journal article Palavras-chave: MiR-98; Col1A1; Apoptosis; Proliferation; HSFBs.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100215
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Protective effects of lycopene on oxidative stress, proliferation and autophagy in iron supplementation rats Biol. Res.
Liu,Chongbin; Wang,Rui; Zhang,Bo; Hu,Chen; Zhang,Hong.
Lycopene is common in diet and known for its antioxidant activities. However, the impact of lycopene on iron metabolism is poorly investigated. In this study, we hypothesize that lycopene can prevent iron-mediated oxidative stress, proliferation and autophagy in liver and use a rat model of nutritional iron supplementation to confirm its intervention in these defence mechanisms. We found that iron supplementation induced cell proliferation predominantly in non parenchymal cells compared with hepatocytes, but not apoptosis. In addition, iron was accumulated within the hepatic lysosomes where it triggered autophagy as evidenced by the formation of autophagic vesicles detected by LC3-B staining. Iron supplementation also induced morphologic alterations of the...
Tipo: Journal article Palavras-chave: Autophagy; Iron supplementation; Lycopene; Oxidative stress; Proliferation.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200011
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Simultaneous silencing of VEGF and KSP by siRNA cocktail inhibits proliferation and induces apoptosis of hepatocellular carcinoma Hep3B cells Biol. Res.
Doan,Chung Chinh; Le,Long Thanh; Hoang,Son Nghia; Do,Si Minh; Van Le,Dong.
BACKGROUND: Vascular endothelial growth factor (VEGF) is involved in the growth of new blood vessels that feed tumors and kinesin spindle protein (KSP) plays a critical role in mitosis involving in cell proliferation. Simultaneous silencing of VEGF and KSP, an attractive and viable approach in cancer, leads on restricting cancer progression. The purpose of this study is to examine the therapeutic potential of dual gene targeted siRNA cocktail on human hepatocellular carcinoma Hep3B cells. RESULTS: The predesigned siRNAs could inhibit VEGF and KSP at mRNA level. siRNA cocktail showed a further downregulation on KSP mRNA and protein levels compared to KSP-siRNA or VEGF-siRNA, but not on VEGF expression. It also exhibited greater suppression on cell...
Tipo: Journal article Palavras-chave: Vascular endothelial cell growth factor (VEGF); Kinesin spindle protein (KSP); SiRNA cocktail; Proliferation; Apoptosis; Hepatocellular carcinoma.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100064
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The possible role of Myosin light chain in myoblast proliferation Biol. Res.
ZHANG,SU-ZHEN; XU,YONG; XIE,HUI-QI; LI,XIU-QUN; WEI,YU-QUAN; YANG,ZHI-MING.
Skeletal muscles have the potential to regenerate by activation of quiescent satellite cells, however, the molecular signature that governs satellite cells during muscle regeneration is not well defined. Myosin light chains (Myls) are sarcomere-related proteins as traditional regulator of muscle contraction. In this report, we studied the possible role of Myl in the proliferation of skeletal muscle-derived myoblasts. Compared to diaphragm-derived myoblasts, the extraocular muscle-derived myoblasts with lower levels of Myl proliferated faster, maintained a longer proliferation phase, and formed more final myotubes. It was found that blockading Myl with anti-Myl antibody or knockdown of Myll by siRNA targeted against Myll could enhance the myoblast...
Tipo: Journal article Palavras-chave: Myosin light chain; Proliferation; Myoblast; Extraocular muscle.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100013
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