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Assessment of four different detergents used to extract membrane proteins from Xylella fastidiosa by two-dimensional electrophoresis BJM
Di Ciero,Luciana; Bellato,Cláudia de M.; Meinhardt,Lyndel W.; Ferrari,Fernanda; Castellari,Rafael R.; Marangoni,Sérgio; Novello,José C..
Four different detergents, ASB 14, SB 3-10, CHAPS and Triton X100, were utilized to determine the optimal detergent for the solubilization of membrane proteins from the phytopathogenic bacterium Xylella fastidiosa. These proteins were differentially solubilized in distinct buffers containing the detergent and subjected to bidimensional electrophoresis within the non-linear pH range of 3-10. The detergents ASB 14 and SB 3-10 were the most effective revealing 221 and 157 spots, respectively. CHAPS and Triton X100 were less effective and revealed only 72 and 43 spots, respectively. MALDI-TOF tryptic peptide mass fingerprinting of 18 excised proteins from the ASB 14 treatment revealed that 83% were membrane proteins and that the theoretical efficiency of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: X. fastidiosa; 2-DE; Extraction; Proteome; Solubilization.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200018
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Cephalic secretion of Arapaima gigas: sex steroids, peptides and proteins suggest roles in chemical communication and parental care. Repositório Alice
TORATI, L. S.; ALBALAT, A.; MESQUITA, P. E.; TAYLOR, J.; MIGAUD, H..
Adults of the Amazon fish Arapaima gigas secrete a fluid from the cephalic canals of the lateral line system, whose biological role(s) along the reproductive cycle are unclear. Up-todate, the biochemical composition of this fluid has been poorly investigated in A. gigas as well as other teleosts. Hence, this study aimed to (1) investigate the potential pheromone release through this cephalic fluid, and (2) to characterise its proteome and peptidome by comparing males and females during and outside the parental care phase.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Peixe; Pirarucu; Arapauma Gigas; Feromônio; Arapaima gigas; Peptides; Proteins; Pheromones; Proteome.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1092864
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Comparative proteome and peptidome analysis of the cephalic fluid secreted by Arapaima gigas (Teleostei: Osteoglossidae) during and outside parental care. Repositório Alice
TORATI, L. S.; MIGAUD, H.; DOHERTY, M. K.; SIWY, J.; MULLEN, W.; MESQUITA, P. E. C.; ALBALAT, A..
Parental investment in Arapaima gigas includes nest building and guarding, followed by a care provision when a cephalic fluid is released from the parents' head to the offspring. This fluid has presumably important functions for the offspring but so far its composition has not been characterised. In this study the proteome and peptidome of the cephalic secretion was studied in parental and non-parental fish using capillary electrophoresis coupled to mass spectrometry (CE-MS) and GeLC-MS/MS analyses. Multiple comparisons revealed 28 peptides were significantly different between males and parental males (PC-males), 126 between females and parental females (PC-females), 51 between males and females and 9 between PC-males and PC-females. Identification...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Arapaima gigas; Peptidome; Pirarucu; Peixe; Proteome; Alloparental behavior.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1078313
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Comparative proteomics between natural Microcystis isolates with a focus on microcystin synthesis. Repositório Alice
TONIETTO, A.; PETRIZ, B. A.; ARAÚJO, W. C.; REIS, A. M. dos; MAGALHÃES, B. S.; FRANCO, O. L..
bitstream/item/156255/1/art3A10.11862F1477-5956-10-38.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Cyanotoxin; Cyanobacteria; Microcystis aeruginosa; Proteome.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/950118
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Different secretory repertoires control the biomineralization processes of prism and nacre deposition of the pearl oyster shell ArchiMer
Marie, Benjamin; Joubert, Caroline; Tayale, Alexandre; Zanella-cleon, Isabelle; Belliard, Corinne; Piquemal, David; Cochennec-laureau, Nathalie; Marin, Frederic; Gueguen, Yannick; Montagnani, Caroline.
Mollusca evolutionary success can be attributed partly to their efficiency to sustain and protect their soft body with an external biomineralized structure, the shell. Current knowledge of the protein set responsible for the formation of the shell microstructural polymorphism and unique properties remains largely patchy. In Pinctada margaritifera and Pinctada maxima, we identified 80 shell matrix proteins, among which 66 are entirely unique. This is the only description of the whole "biomineralization toolkit" of the matrices that, at least in part, is thought to regulate the formation of the prismatic and nacreous shell layers in the pearl oysters. We unambiguously demonstrate that prisms and nacre are assembled from very different protein repertoires....
Tipo: Text Palavras-chave: Mantle; Mollusk shell matrix proteins; Proteome; Transcriptome; Evolution.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00118/22959/20922.pdf
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Egg quality in domesticated and wild seabass (Dicentrarchus labrax): A proteomic analysis ArchiMer
Crespel, Amlie; Rime, Helene; Fraboulet, Erwann; Bobe, Julien; Fauvel, Christian.
In order to improve Our understanding of factors determining egg quality in fish, seabass egg batches obtained from 34 females held under standard conditions, were assessed for biological performances and a fraction from each batch was subjected to proteomic analysis. Comparison of proteomic patterns from eggs of different quality revealed differential protein spots among which one was identified as Nucleoside Diphosphate Kinase, a highly conserved protein involved in cell proliferation. This study shows that molecular markers may help understand factors determining egg quality in fish.
Tipo: Text Palavras-chave: Seabass; NDK; Egg quality; Proteome.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-6555.pdf
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High pressure-sensitive gene expression in Lactobacillus sanfranciscensis BJMBR
Vogel,R.F.; Pavlovic,M.; Hörmann,S.; Ehrmann,M.A..
Lactobacillus sanfranciscensis is a Gram-positive lactic acid bacterium used in food biotechnology. It is necessary to investigate many aspects of a model organism to elucidate mechanisms of stress response, to facilitate preparation, application and performance in food fermentation, to understand mechanisms of inactivation, and to identify novel tools for high pressure biotechnology. To investigate the mechanisms of the complex bacterial response to high pressure we have analyzed changes in the proteome and transcriptome by 2-D electrophoresis, and by microarrays and real time PCR, respectively. More than 16 proteins were found to be differentially expressed upon high pressure stress and were compared to those sensitive to other stresses. Except for one...
Tipo: Info:eu-repo/semantics/article Palavras-chave: High pressure; Lactobacillus sanfranciscensis; Proteome; Transcriptome; Stress response.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800013
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Proteome analysis of gene expression in rice (Oryza sativa L.) leaves during drought stress and recovery International Rice Research Institute
Salekdeh, Seyed Ghasem Hosseini.
xi, 93 l. : ill. Thesis (Ph.D.) -- University of the Philippines at Los Baños
Tipo: Thesis Palavras-chave: Rice; Plant genetics; Proteome; Gene expression; Leaves; Water stress; Drought tolerance.
Ano: 2002 URL: http://hdl.handle.net/123456789/418
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Proteome of fraction from Tityus serrulatus venom reveals new enzymes and toxins J. Venom. Anim. Toxins incl. Trop. Dis.
Amorim,Fernanda Gobbi; Longhim,Heloisa Tavoni; Cologna,Camila Takeno; Degueldre,Michel; Pauw,Edwin De; Quinton,Loïc; Arantes,Eliane Candiani.
Abstract Background: Tityus serrulatus venom (Ts venom) is a complex mixture of several compounds with biotechnological and therapeutical potentials, which highlights the importance of the identification and characterization of these components. Although a considerable number of studies have been dedicated to the characterization of this complex cocktail, there is still a limitation of knowledge concerning its venom composition. Most of Ts venom studies aim to isolate and characterize their neurotoxins, which are small, basic proteins and are eluted with high buffer concentrations on cation exchange chromatography. The first and largest fraction from carboxymethyl cellulose-52 (CMC-52) chromatography of Ts venom, named fraction I (Fr I), is a mixture of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tityus serrulatus; Scorpion venom; Enzymes; Proteases; ACE inhibitors; Proteome.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100308
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Proteome-wide evolutionary analysis reveals lineage-specific adaptations and improves funtional annotation of Schistosoma mansoni proteins. Repositório Alice
SILVA, L.; MARCET-HOUBEN, M.; NAHUM, L.; ZERLOTINI, A.; GALBADÓN, T.; OLIVEIRA, G..
In this context, comparative proteomic analysis can shed new light on the evolutionary processes that shaped hostparasite interaction over evolutionary time. Taking advantage of the benefits provided by a largescale phylogenetic analysis, in the present work, we reconstructed the evolutionary history of each protein encoded in the S. mansoni genome in comparison with other 12 taxa to gain insights into lineage-specific evolutionary events, potentially related to the parasitic lifestyle, as well as to improve functional annotation. Results The collection of trees reconstructed in this work includes 7,964 phylogenies, which comprises the evolutionary histories of all parasite proteins and their homologs across 12 other organisms. This analysis allowed a...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Genoma; Proteoma; Schistosoma mansoni; Proteome; Genomics.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/946562
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Proteomic Analysis of the Chlorophyta Dunaliella New Strain AL-1 Revealed Global Changes of Metabolism during High Carotenoid Production ArchiMer
Ben Amor, Faten; Elleuch, Fatma; Ben Hlima, Hajer; Garnier, Matthieu; Saint-jean, Bruno; Barkallah, Mohamed; Pichon, Chantal; Abdelkafi, Slim; Fendri, Imen.
The green microalgae Dunaliella genus is known for the production of high added value molecules. In this study, strain AL-1 was isolated from the Sebkha of Sidi El Hani (Sousse, Tunisia). This isolate was identified both morphologically and genetically via 18S rRNA gene sequence as a member of the genus Dunaliella. Strain AL-1 was found to be closely related to Dunaliella salina, Dunaliella quartolecta and Dunaliella polymorpha with more than 97% similarity. Response surface methodology was used to maximize carotenoid production by strain AL-1 by optimizing its growth conditions. The highest carotenoid content was obtained at salinity: 51, light intensity: 189.89 μmol photons·m−2·s−1, and nitrogen: 60 mg·L−1. Proteomic profiling, using two-dimensional gel...
Tipo: Text Palavras-chave: Proteome; Dunaliella; 18S rRNA gene; Carotenoids; Bidimensional gel electrophoresis; Mass spectrometry.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00402/51398/51973.pdf
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Proteomic and metabolomic analysis of a drought tolerant soybean cultivar from Brazilian savanna. Repositório Alice
LIMA, L. L.; BALBI, B. P.; MESQUITA, R. O.; SILVA, J. C. F. da; COUTINHO, F. S.; CARMO, F. M. S.; VITAL, C. E.; MEHTA, A.; LOUREIRO, M. E.; FONTES, E. P. B.; BARROS, E. G.; RAMOS, H. J. O..
bitstream/item/203624/1/CBGG-Lima-et-al-Humberto-2019.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Phosphoproteome; Soybeans; Drought tolerance; Proteome; Metabolome.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113447
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Rooteomics: the challenge of discovering plant defense-related proteins in roots. Repositório Alice
MEHTA, A.; MAGALHÃES, B. S.; SOUZA, D. S. L.; VASCONCELOS, E. A. R.; SILVA, L. P.; GROSSI-DE-SÁ, M. F.; FRANCO, O. L.; COSTA, P. H. A. da; ROCHA, T. L..
bitstream/item/177327/1/SP-19559-ID-30491.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Root; Planta; Proteína; Raiz; Mass spectrometry; Proteome.
Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/170844
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The inhibitory effect of calcium on Cylindrospermopsis raciborskii (cyanobacteria) metabolism BJM
Carneiro,Ronaldo Leal; Alípio,Ana Carla Nascimento; Bisch,Paulo Mascarello; Azevedo,Sandra Maria Feliciano de Oliveira e; Pacheco,Ana Beatriz Furlanetto.
Cylindrospermopsis raciborskii (Woloszynska) Seenaya & Subba Raju is a freshwater cyanobacterium of worldwide distribution. In the North-eastern region of Brazil many eutrophic water reservoirs are characterized by the dominance of C. raciborskii, with recurrent occurrence of blooms. These water bodies have high conductivity due to a high ionic concentration, and are defined as hard (with high values of CaCO3). In this study, we investigated the long-term effect (12 days) of high calcium concentration (8 mM Ca2+) on C. raciborskii (T3 strain) growth, morphology, toxin content, and metabolism. Changes in protein expression profiles were investigated by proteomic analysis using 2D gel electrophoresis and mass spectrometry. A continued exposure to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Calcium; Cylindrospermopsis; Paralitic shellfish toxin; Proteome.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400042
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Trypanosoma cruzi: analysis of two different strains after piplartine treatment BJID
Vieira,Gabriela Alves Licursi; Silva,Marco Túlio Alves da; Regasini,Luis Octávio; Cotinguiba,Fernando; Laure,Helen Julie; Rosa,José César; Furlan,Maysa; Cicarelli,Regina Maria Barretto.
ABSTRACT The hemoflagellate protozoan, Trypanosoma cruzi, mainly transmitted by triatomine insects through blood transfusion or from mother-to-child, causes Chagas' disease. This is a serious parasitic disease that occurs in Latin America, with considerable social and economic impact. Nifurtimox and benznidazole, drugs indicated for treating infected persons, are effective in the acute phase, but poorly effective during the chronic phase. Therefore, it is extremely urgent to find innovative chemotherapeutic agents and/or effective vaccines. Since piplartine has several biological activities, including trypanocidal activity, the present study aimed to evaluate it on two T. cruzi strains proteome. Considerable changes in the expression of some important...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trypanosomatids; Proteome; Piplartine; Therapeutic targets.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000300208
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