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Registros recuperados: 61
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A comparative analysis between (32P) and digoxigenin-labelled single-copy probes for RFLP detection in wheat Inra
Lu, Y.H.; Merlino, M.; Isaac, P.G.; Staccy, J.; Bernard, M.; Leroy, P..
Le polymorphisme de longueur des fragments de restriction (PLFR) a été largement utilisé pour l’établissement de cartes génétiques des plantes et pour diverses études génétiques. Bien qu’elle présente d’intéressantes potentialités et perspectives pour les sélectionneurs, l’utilisation des éléments radioactifs dans cette technique a limité son application en routine. Nous avons modifié et amélioré une des méthodes non radioactives commercialement disponibles, le marquage à la digoxigénine (Boehringer Mannheim), pour l’analyse d’un génome polyploïde tel que le blé. Les résultats obtenus sont comparables à ceux de la méthode radioactive. Les procédures expérimentales sont décrites en détail. Cette méthode non radioactive devrait faciliter tous les types...
Tipo: Journal Article Palavras-chave: RFLP; DIGOXIGENINE; SONDE MONOCOPIE; P32; MARQUAGE NON RADIOACTIF.
Ano: 1994 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9500002393045103&uri=/notices/prodinra1/2009/11/
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A Comparative Study of Willaertia magna (Free-Living Amoeba) from Different Geographic Areas Using Whole-cell and Small-subunit rDNA Restriction Fragment Length Polymorphisms OAK
Kilvington, Simon; Stevens, Clair; Ebert, Frank; Michel, Rolf; Beeching, John.
Palavras-chave: Free-living amoeba; Willaertia magna; Naegleria fowlen; RFLP.
Ano: 1995 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/230
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A comparison of biological, molecular and enzymatic markers to investigate variability within Microdochium nivale (Fries) Samuels and Hallett Inra
Maurin, N.; Rezanoor, H.N.; Lamkadmi, Z.; Some, A.; Nicholson, P..
Les variations au sein d’une collection de souches européennes de Microdochium nivale isolées de plusieurs hôtes sont étudiées sur la base de la morphologie des conidies, des profils d’estérases et de RFLP de la région des ITS de l’ADN ribosomique, de l’agressivité sur des plantules de blé et de la capacité à produire des périthèces in vitro. Un groupe homogène est différencié à la fois par les profils d’estérases et les RFLP des ITS de l’ADN-r. Les isolats de ce groupe possèdent des profils d’ITS identiques aux isolats de référence de la variété majus utilisés par d’autres auteurs et sont, par conséquent, assignés à la variété majus. Les isolats à l’intérieur du groupe homogène majus ont été collectés à travers l’Europe, ce qui démontre que ce groupe...
Tipo: Journal Article Palavras-chave: MICRODOCHIUM NIVALE; RFLP; PROFIL BIOCHIMIQUE; PLANTE HOTE; MARQUEUR; VARIABILITE INTRASPECIFIQUE; CONIDIE; MARQUAGE ENZYMATIQUE; ISOLAT.
Ano: 1995 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9500012408050369&uri=/notices/prodinra1/2010/01/
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A simplified procedure for terminal restriction fragment length polymorphism analysis of the soil bacterial community to study the effects of pesticides on the soil microflora using 4,6-dinitroorthocresol as a test case Inra
Rousseaux, S.; Hartmann, A.; Rouard, N.; Soulas, G..
We have studied the structural effects of application to the soil of a potentially detrimental herbicide, 4,6-dinitroorthocresol (DNOC) by analysing amplified ribosomal DNA restriction analysis (ARDRA) and terminal restriction fragment length polymorphism (T-RFLP) signatures of 16S rDNA fragments of culturable bacterial communities isolated from diluted soil suspensions. This approach has the potential to reveal changes induced by stressing the soil microflora with DNOC. This paper shows that, whereas only few changes of the ARDRA and T-RFLP profiles result from ageing of the soil, treatment of the soil with DNOC induces major modifications of these profiles. Therefore, for the practical purpose of pesticide registration, ARDRA and T-RFLP analysis...
Tipo: Journal Article Palavras-chave: 4 6-DINITROORTHOCRESOL; COMMUNAUTE MICROBIENNE; RFLP.
Ano: 2003 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0400035298104675&uri=/notices/prodinra1/2010/12/
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Análise de polimorfismo do gene da somatotropina em vacas Nelore e seu efeito sobre o peso à desmama de suas progênies Arq. Bras. Med. Vet. Zootec.
Faria,F.J.C.; Guimarães,S.E.F.; Lima,R.M.G.; Mourão,G.B.; Pinheiro,L.E.L..
Informações sobre peso à desmama de um rebanho Nelore foram utilizadas após ajuste para idade padrão de 205 dias, sexo da cria, idade da mãe, touro e mês de desmama, para separar as reprodutrizes em dois grupos, cujos filhos diferiam nesse peso. As médias ajustadas pelo método dos quadrados mínimos foram para os grupos pesado (P) e leve (L) de 163,21± 2,18kg e 134,44± 2,18kg, respectivamente, com 41 animais em cada grupo. Essas reprodutrizes foram submetidas à coleta de sangue para estudo de polimorfismos do gene da somatotropina bovina, pela técnica de PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism). A amplificação de uma região entre o éxon III e V do gene da somatotropina permitiu analisar dois sítios de restrição. Para o...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovino; Somatotropina; PCR; RFLP; Polimorfismo genético.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000600011
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Análise de polimorfismos do gene da beta-lactoglobulina em vacas da raça Nelore e efeitos sobre o peso à desmama de suas progênies Arq. Bras. Med. Vet. Zootec.
Faria,F.J.C.; Guimarães,S.E.F.; Mourão,G.B.; Lima,R.M.G.; Pinheiro,L.E.L..
Informações sobre peso à desmama de bezerros Nelore foram utilizadas após ajuste para idade padrão aos 205 dias, sexo, idade da mãe, touro e mês de desmama, para separar as reprodutrizes em dois grupos, segundo o peso de suas crias. As médias de peso dos bezerros ajustadas pelo método dos quadrados mínimos e erros-padrão (LSM± SE) foram para os grupos pesados (P) e leves (L) 163,21± 2,18kg e 134,44± 2,18kg, respectivamente, com 41 animais em cada grupo. Essas reprodutrizes foram submetidas a coleta de sangue para estudo de polimorfismos do gene da beta-lactoglobulina, por meio da técnica de PCR-RFLP. A amplificação e a digestão de um fragmento do gene da beta-lactoglobulina entre o éxon II e III identificou os genótipos 1AA, 24AB e 56BB, com as freqüências...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovino; Nelore; Beta-lactoglobulina; PCR; RFLP; Polimorfismo genético.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000300016
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Association of PIT1 genotypes with growth traits in Canchim cattle Scientia Agricola
Carrijo,Sônia Mara; Alencar,Maurício Mello de; Toral,Fábio Luiz Buranelo; Regitano,Luciana Correia de Almeida.
Use of genetic markers can aid on the identification of animals with highest breeding values in beef cattle. The PIT1 gene codes for the Pit-1 transcription factor is essential for the activation of prolactin, growth hormone and PIT1 genes. This research is an investigation of the effect of PIT1-HinfI polymorphism on growth traits of 509 Canchim animals, from two lineages, GG1 and GG2. PIT1 genotypes were identified through PCR-RFLP. Genotype effect on phenotypic values for birth weight (BW), standardized weaning weight (W240), weight at 12 months of age (W365), and the average daily weight gain from birth to weaning (AGBW), and from weaning to 12 months of age (AGW12) were analyzed by the least squares method. Effects of the interaction between the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: RFLP; Bovine; Pituitary factor 1; Candidate gene.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000200002
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Biochemical and molecular investigations on qualitative and quantitative Hb polymorphism in the river buffalo (Bubalus bubalis L.) population reared in Southern Italy Genet. Mol. Biol.
Iorio,Mario; Vincenti,Donatella; Annunziata,Mario; Rullo,Rosario; Bonamassa,Raffaele; Di Luccia,Aldo; Pieragostini,Elisa.
On 398 river buffalo samples, randomly collected in distinct breeding areas of the Campania region, high-resolution analytical systems were used to identify both qualitative and quantitative variations of the Hb phenotype. Polyacrylamide gel isoelectric focusing and HPLC were used to determine the ratio between HBA1 and HBA2 globin chains; restriction endonuclease analysis was performed to assess whether quantitative variations in Hb bands were related to an unusual number of a-globin genes. In the two buffalo subpopulations, allele frequencies of the alpha and beta globin systems were calculated, and F statistics (FIS, FIT and FST) were estimated as parameters of genetic diversity. The results suggest that: i) as shown by RFLP analysis, only a couple of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phenotype; RFLP; Globin genes; Haplotype; Gene frequencies.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200007
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Bovine Herpesvirus 4 in Parana State, Brazil: case report, viral isolation, and molecular identification BJM
Kruger,Ernesto Renato; Penha,Tania Regina; Stoffelo,Daura Regina Eira; Roehe,Paulo Michel; Ribeiro,Magda Costa; Soccol,Vanete Thomaz.
Bovine Herpesvirus 4 (BoHV-4) is a member of Gammaherpesvirinaesub-family and belongs to genus Rhadinovirus. This virus has been associated with different clinical manifestations and research activity has put forward a strong correlation among virus infection, postpartum metritis, and abortion. The goal of this work was to characterize a virus strain isolate from a cow’s uterine outflow. From swabs drawn of uterine secretion, a virus strain was isolated and characterized by its cytopathology, morphology, and molecular biology approaches. In culture there was CPE development, characterized mainly by long strands with several small balloons along them, radiated from infected cells. Electron microscopy analysis revealed virus particles that had icosahedrical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: BoHV-4 virus; PCR; RFLP; Fingerprint.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100279
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Caractérisation biologique et moléculaire des isolats de Cucumber mosaic virus (CMV) dans les cultures de piments en Tunisie Inra
Ben Tamarzizt, H.; Girardot, G.; Fakhfakh, H.; Jacquemond, M..
Tipo: Conference Paper Palavras-chave: WATERMELON MOSAIC VIRUS; WMV; RFLP.
Ano: 2011 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201164cf646d&uri=/notices/prodinra1/2011/02/
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Caracterização molecular de animais da raça Nelore utilizando microssatélites e genes candidatos R. Bras. Zootec.
Tambasco,Daniella Debenedetti; Alencar,Maurício Mello de; Coutinho,Luiz Lehmann; Tambasco,Antonio Junqueira; Tambasco,Marina Debenedetti; Regitano,Luciana Correia de Almeida.
No presente trabalho, 180 fêmeas da raça Nelore, provenientes de oito rebanhos, foram analisadas quanto aos marcadores microssatélites TEXAN15, BM1224 e CSFM50 e aos polimorfismos de comprimento de fragmentos de restrição (RFLP) nos locos k-caseína, beta-lactoglobulina e hormônio de crescimento (GH). Com exceção de GH, todos os marcadores foram polimórficos na amostra estudada. Os valores de heterozigosidade, diversidade gênica, conteúdo de informação polimórfica (PIC) e probabilidade de exclusão de paternidade (PE) foram estimados. Os maiores valores de PIC (0,685) e PE (0,521) foram obtidos para o marcador BM1224.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovino; DNA; Marcador; Microssatélite; Nelore; RFLP.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982000000400014
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Caracterización de ADN de clones de papa e identificación de fitoplasmas asociados al síndrome de la punta morada Agrociencia
Alarcón-Rodríguez,Norma M.; Lozoya-Saldaña,Héctor; Valadez-Moctezuma,Ernestina.
En muchas áreas sembradas con papa en México se manifiesta el síndrome de la punta morada, que reduce el rendimiento y la calidad del tubérculo. Un factor principal de este síndrome es la presencia de fitoplasmas y el inadecuado manejo de su insecto vector. Una opción para controlar esta enfermedad es generar genotipos de papa resistentes y un diagnóstico oportuno para evitar su diseminación. Por ello los objetivos de este trabajo fueron detectar fitoplasmas asociados a la punta morada presentes en clones de papa asintomáticos, pero que estuvieron expuestos a la misma por cuatro ciclos, e identificar la relación hospedero-patógeno. Al final del ciclo de cultivo del 2005, fueron recolectadas hojas de 18 clones de papa sin síntomas, en Toluca, Estado de...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Punta morada; Resistencia genética; RAPDs; RFLP.
Ano: 2009 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952009000400003
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Characteristics of random amplified polymorphic DNA markers discriminating Helianthus annuus inbred lines Inra
Teulat, B.; Zhang, Y.X..
Nous avons testé l’utilisation de marqueurs RAPD (random amplified polymorphic DNA) pour permettre la discrimination de lignées de tournesol cultivé Helianthus annuus. En testant 100 amorces décamères sur les ADN de 4 lignées, nous obtenons 40 amorces révélant un polymorphisme. Nous montrons qu’en sélectionnant les amorces donnant des signaux reproductibles on obtient 14 marqueurs RAPD permettant de caractériser des lignées de tournesol. L’étude moléculaire des fragments amplifiés montre que ces marqueurs sont de type «présence/absence» : un locus est caractérisé par une bande chez une lignée et l’absence de bande chez les autres lignées. Des expériences de Southern utilisant des fragments RAPD polymorphes comme sondes produisent des signaux d’hybridation...
Tipo: Journal Article Palavras-chave: RAPD; RFLP; MARQUEUR GENETIQUE.
Ano: 1994 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9500019671048235&uri=/notices/prodinra1/2009/11/
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Characterization and lytic activity of Pseudomonas fluorescens phages from sewage BJM
Radhakrishnan,Ananthi; Ananthasubramanian,M.
Pseudomonas fluorescens phages from sewage were tested against P. fluorescens isolates of soil and sewage. The phages were characterized as to host range, morphology, structural proteins and genome fingerprint. Of the seven phages isolated, one was found to be abundant in sewage (5.9×10(7) pfu/mL), having broad host range, and distinct protein and DNA profile when compared to the other six phages. DNA restriction and protein profiles of the phages and their morphology indicate the diversity in the sewage environment. None of the isolates from the rhizosphere regions of various cultivated soils were susceptible to phages isolated from sewage.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas fluorescens; Phages; RFLP; AFM; Protein analysis.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100042
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Clonal diversity and antimicrobial resistance of Enterococcus faecalis isolated from endodontic infections Electron. J. Biotechnol.
Suliman Al-Badah,Abdulhakim; S.S. Ibrahim,Abdelnasser; Al-Salamah,Ali A; Ibrahim,Shebl Salah S.
Background Enterococcus faecalis is considered to be one of most prevalent species in the oral cavity, particularly in endodontic infections. The aim of the present study was to investigate the prevalence of E. faecalis in dental root canals, clonal diversity by restriction fragment length polymorphism (RFLP) and randomly amplified polymorphic DNA (RAPD-PCR) analysis, and the antibiotic susceptibility of E. faecalis isolates. Results Among the bacterial strains isolated from dental root canal specimens (n = 82), E. faecalis was determined to have the highest prevalence followed by Streptococcus viridians, Leuconostoc mesenteroides, Staphylococcus aureus, Streptococcus mitis, and Pediococcus pentosaceus. Cluster analysis of RAPD-PCR and RFLP patterns of the...
Tipo: Journal article Palavras-chave: Antimicrobial susceptibility; Endodontic infection; Enterococcus faecalis; Genotype; RAPD; RFLP.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300006
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Comparative molecular analysis of Herbaspirillum strains by RAPD, RFLP, and 16S rDNA sequencing Genet. Mol. Biol.
Soares-Ramos,Juliana R.L.; Ramos,Humberto J.O.; Cruz,Leonardo M.; Chubatsu,Leda S.; Pedrosa,Fábio O.; Rigo,Liu U.; Souza,Emanuel M..
Herbaspirillum spp. are endophytic diazotrophic bacteria associated with important agricultural crops. In this work, we analyzed six strains of H. seropedicae (Z78, M2, ZA69, ZA95, Z152, and Z67) and one strain of H. rubrisubalbicans (M4) by restriction fragment length polymorphism (RFLP) using HindIII or DraI restriction endonucleases, random amplified polymorphic DNA (RAPD), and partial sequencing of 16S rDNA. The results of these analyses ascribed the strains studied to three distinct groups: group I, consisting of M2 and M4; group II, of ZA69; and group III, of ZA95, Z78, Z67, and Z152. RAPD fingerprinting showed a higher variability than the other methods, and each strain had a unique electrophoretic pattern with five of the six primers used....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Herbaspirillum; RAPD; RFLP; Phylogeny; 16S rDNA.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400019
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Comparative study of RFLP-IS6110 and MIRU-VNTR from Mycobacterium tuberculosis isolated in the state of Minas Gerais, Brazil BJM
Augusto,Cláudio José; Carvalho,Wânia da Silva; Almeida,Isabela Neves de; Figueiredo,Lida Jouca de Assis; Dantas,Nayanne Gama Teixeira; Suffys,Philip Noel; Miranda,Silvana Spíndola de.
Abstract DNA genotyping of Mycobacterium tuberculosis has been widely applied in the understanding of disease transmission in many countries. The purpose of this study was to genotype the strains of M. tuberculosis isolated in patients with new tuberculosis (TB) cases in Minas Gerais, as well as to compare the similarity, discriminatory power, and agreement of the clusters between the IS6110 Restriction Fragment Length Polymorfism (RFLP) and 12 loci Variable Number Tandem Repeat - Mycobacterial Interspersed Repetitive Units (MIRU-VNTR) techniques. It was observed that 32% (66/204) of the isolated strains in the RFLP-IS6110 and 50.9% (104/204) of the isolated strains in the MIRU-VNTR presented a similarity of equal to or above 85%. The RFLP-IS6110 and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium tuberculosis; RFLP; VNTR loci.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300641
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Comparison of multiple genotyping methods for the identification of the cancer predisposing founder mutation p.R337H in TP53 Genet. Mol. Biol.
Fitarelli-Kiehl,Mariana; Macedo,Gabriel S.; Schlatter,Rosane Paixão; Koehler-Santos,Patricia; Matte,Ursula da Silveira; Ashton-Prolla,Patricia; Giacomazzi,Juliana.
Abstract Germline mutations in the TP53 gene are associated with Li-Fraumeni and Li-Fraumeni-Like Syndromes, characterized by increased predisposition to early-onset cancers. In Brazil, the prevalence of the TP53-p.R337H germline mutation is exceedingly high in the general population and in cancer-affected patients, probably as result of a founder effect. Several genotyping methods are used for the molecular diagnosis of LFS/LFL, however Sanger sequencing is still considered the gold standard. We compared performance, cost and turnaround time of Sanger sequencing, PCR-RFLP, TaqMan-PCR and HRM in the p.R337H genotyping. The performance was determined by analysis of 95 genomic DNA samples and results were 100% concordant for all methods. Sequencing was the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: TP53-p.R337H; RFLP; TaqMan; HRM; Sanger Sequencing.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200203
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Constitution d'une génothèque destinée à l'identification des génomes mitochondriaux et recherche de nouvelles séquences mitochondriales chez les végétaux supérieurs Inra
Lejeune, B.; Pereira de Souza, A.; Haouazine, N.; Jubier, M.F.; Besin, E.; Lancelin, D.; Pelletier, G..
Le génome mitochondrial (mt) des plantes supérieures, dont la taille peut varier entre 200 et 560 kb, est composé d’une population hétérogène de molécules de tailles variables pouvant se recombiner grâce à la présence de séquences répétées. Les gènes présents sont constitués de séquences codantes très conservées et peuvent présenter des caractéristiques structurales comme la présence d’introns interrompus conduisant à un épissage en trans. Ces séquence codantes peuvent être utilisées comme sondes pour caractériser le polymorphisme entre variétés ou espèces. La recherche de nouvelles séquences codantes utilisables nous a amenés à identifier et caractériser 2 gènes mt nouveaux, nad5 et nad6.
Tipo: Journal Article Palavras-chave: GENE NAD5; GENE NAD6; CONSERVATION DE SEQUENCE; RFLP; EPISSAGE EN TRANS; EDITION DES ARN; ADN MITOCHONDRIAL; GENOTHEQUE; SEQUENCE NUCLEOTIDIQUE; PLANTE; POLYMORPHISME GENETIQUE.
Ano: 1994 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9500023518052077&uri=/notices/prodinra1/2007/11/
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Contamination de l'environnement littoral par les rotavirus du groupe A. Détection par amplification enzymatique en chaîne et analyse du polymorphisme de restriction de séquences virales dans les eaux de surfaces et les coquillages ArchiMer
Dubois, Eric.
Les rotavirus font partie des principaux virus entériques humains susceptibles d'être rencontrés dans l'environnement. Leur mise en évidence par culture cellulaire dans des prélèvements est longue et peu sensible, ce qui explique le manque de données sur la contamination du littoral. La RT-seminested PCR a été développée pour pallier ces inconvénients. Après une mise au point méthodologique cette technique a été utilisée lors d'études in vitro et in situ. Les études in vitro sur le devenir des rotavirus en eau de mer, suivi par RT-PCR quantitative et par multiplication en culture cellulaire, montrent que dans des conditions proches de celles du milieu naturel (eau de mer non stérile et à une température relativement basse), la détection d'ARN viral peut...
Tipo: Text Palavras-chave: Rotavirus; Contamination; Coquillages; Eaux de stations d'épuration; Echantillons cliniques; RT-PCR; RFLP; Epidémiologie moléculaire.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/00442/55376/56894.pdf
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