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Anethole reduces oxidative stress and improves in vitro survival and activation of primordial follicles BJMBR
Sá,N.A.R.; Bruno,J.B.; Guerreiro,D.D.; Cadenas,J.; Alves,B.G.; Cibin,F.W.S.; Leal-Cardoso,J.H.; Gastal,E.L.; Figueiredo,J.R..
Primordial follicles, the main source of oocytes in the ovary, are essential for the maintenance of fertility throughout the reproductive lifespan. To the best of our knowledge, there are no reports describing the effect of anethole on this important ovarian follicle population. The aim of the study was to investigate the effect of different anethole concentrations on the in vitro culture of caprine preantral follicles enclosed in ovarian tissue. Randomized ovarian fragments were fixed immediately (non-cultured treatment) or distributed into five treatments: α-MEM+ (cultured control), α-MEM+ supplemented with ascorbic acid at 50 μg/mL (AA), and anethole at 30 (AN30), 300 (AN300), or 2000 µg/mL (AN2000), for 1 or 7 days. After 7 days of culture, a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant; Anethole; ROS; Primordial follicle; Caprine.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800602
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Antiproliferative activity of marine stingray Dasyatis sephenvenom on human cervical carcinoma cell line J. Venom. Anim. Toxins incl. Trop. Dis.
Rajeshkumar,RK; Vennila,R; Karthikeyan,S; Prasad,N Rajendra; Arumugam,M; Velpandian,T; Balasubramaniam,T.
AbstractBackgroundVenoms comprise mixtures of numerous bioactive compounds that have a wide range of pharmacologic actions. Toxins from venomous animals have attracted the attention of researchers because of their affinity for primary sites responsible for lethality and their efficacy at extremely low concentrations. The venoms of marine stingrays have not been extensively studied and limited data is available on them. The present study aims to evaluate the antiproliferative and biochemical properties of the venom obtained from a species of marine stingray (Dasyatis sephen) on human cervical cancer cell line HeLa.MethodsThe antiproliferative effect of D. sephen venom was determined by MTT assay, and the oxidative stress was determined by lipid peroxidation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Marine organisms; Membrane potential; Oxidative stress; ROS; Stingray; Venom.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100345
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Carnauba wax p-methoxycinnamic diesters: Characterisation, antioxidant activity and simulated gastrointestinal digestion followed by in vitro bioaccessibility. Repositório Alice
FREITAS, C.A.S.; VIEIRA, I.G.P.; SOUSA, P.H.M; MUNIZ, C. R.; GONZAGA, M.L.da. C.; GUEDES, M.I.F..
2015
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Carnauba; Cinnamic acid diesters; Antioxidant; ROS.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1031039
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Cassava postharvest physiological deterioration: a complex phenomenon involving calcium signaling, reactive oxygen species and programmed cell death. Repositório Alice
DJABOU, A. S. M.; CARVALHO, L. J. C. B.; LI, Q. S.; NIEMENAK, N.; CHEN, S..
bitstream/item/181945/1/11738-2017-Article-2382.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Postharvest physiological deterioration; ROS; Programmed cell death; Crosstalk; Manihot Esculenta; Calcium signaling.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1094527
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Changes in Photosynthesis and Oxidative Stress in Wheat Plants Submmited to Herbicides Application Planta Daninha
AGOSTINETTO,D.; PERBONI,L.T.; LANGARO,A.C.; GOMES,J.; FRAGA,D.S.; FRANCO,J.J..
The use of herbicides, even in tolerant crops, can cause stress evidenced by increase phytotoxicity affecting growth and development. The objectives of this study were to evaluate herbicides effect from different mechanisms of action in photosynthetic and oxidative stress parameters, as well visual phytotoxicity and wild radish control in wheat crop, cultivar Quartzo. Two trials were conducted where the first one evaluated the photosynthetic parameters on wheat plants in two seasons collection, following the application of herbicides bentazon, clodinafop, iodosulfuron, metribuzin, metsulfuron and 2,4-D; and the second one evaluated wild radish (Raphanus sativus) control, wheat phytotoxicity and yield due to bentazon, iodosulfuron, metribuzin, metsulfuron...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum; Selectivity; ROS; Antioxidant enzymes.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582016000100001
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Fruits and barks extracts of Zanthozyllum heitzii a spice from Cameroon induce mitochondrial dependent apoptosis and Go/G1 phase arrest in human leukemia HL-60 cells Biol. Res.
Pieme,Constant Anatole; Santosh,Guru Kumar; Tekwu,Emmanuel Mouafo; Askun,Tülin; Aydeniz,Hatice; Ngogang,Jeanne Yonkeu; Bhushan,Shashi; Saxena,Ajit Kumar.
BACKGROUND: Zanthoxylum heitzii is a spice used to prepare several dishes and to treat tumors, syphilis, malaria, cardiac palpitations, urogenital infections in the west region of Cameroon, but the antitumor mechanisms and chemical composition are not yet investigated. This study was aimed to determine the antiproliferative effects of four extracts from the fruits and barks of Zanthoxyllum heitzii (Rutaceae) on apoptosis in human promyelocytic cells, their mechanisms and the chemical composition. The 3-(4, 5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the fifty percent inhibition (IC50) concentration of the cell lines after treatment. The effect on morphology was observed using a light or fluorescence...
Tipo: Journal article Palavras-chave: Zanthozyllum heitzii; Syringic acid; Apoptosis; HL-60 cells; ROS; Mitochondrial membrane potential.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100048
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Genetically based resistance to summer mortality in the Pacific oyster (Crassostrea gigas) and its relationship with physiological, immunological characteristics and infection processes ArchiMer
Samain, Jean-francois; Degremont, Lionel; Soletchnik, Patrick; Haure, Joel; Bedier, Edouard; Ropert, Michel; Moal, Jeanne; Huvet, Arnaud; Bacca, Helene; Van Wormhoudt, A; Delaporte, Maryse; Costil, K; Pouvreau, Stephane; Lambert, Christian; Boulo, Viviane; Soudant, Philippe; Nicolas, Jean-louis; Leroux, Frederique; Renault, Tristan; Gagnaire, Beatrice; Geret, Florence; Boutet, Isabelle; Burgeot, Thierry; Boudry, Pierre.
Summer mortality of Pacific oysters is known in several countries. However no specific pathogen has been systematically associated with this phenomenon. A complex combination of environmental and biological parameters has been suggested as the cause and is now starting to be identified. A high genetic basis was found for survival in oysters when a first generation (G1) was tested in three sites during summer. This paper presents a synthesis on physiological characteristics of two selected groups ('R' and 'S', from families selected for resistance and susceptibility to summer mortality respectively), of the second and third generations. R and S showed improvement or reduction of survival compared with the control in both field and laboratory trials...
Tipo: Text Palavras-chave: ROS; Temperature; Stress; Hemocytes; Reproduction; Genetic selection; Resistant oyster; Crassostrea gigas; Pacific Oyster; Summer mortality.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2809.pdf
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Geração e desintoxicação enzimática de espécies reativas de oxigênio em plantas Ciência Rural
Barbosa,Marta Ribeiro; Silva,Marina Medeiros de Araújo; Willadino,Lilia; Ulisses,Claudia; Camara,Terezinha Rangel.
As condições de estresse biótico e abiótico impostas às plantas induzem a superprodução de espécies reativas de oxigênio (ROS), podendo causar danos às estruturas celulares e mesmo acarretar a morte da planta. As respostas bioquímicas e fisiológicas de plantas superiores ao estresse oxidativo incluem um eficiente sistema de defesa antioxidante, que envolve a atividade das enzimas superóxido dismutase, catalase, ascorbato peroxidase, peroxirredoxinas, dentre outras, além de metabólitos não enzimáticos, que, de forma conjunta, atuam na eliminação das ROS e na redução do dano oxidativo. Nesta revisão, serão abordados os principais sítios de produção de ROS e a ação de algumas enzimas do sistema de defesa antioxidante em plantas.
Tipo: Info:eu-repo/semantics/article Palavras-chave: ROS; Peróxido de hidrogênio; Radical superóxido; Enzimas antioxidantes.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000300011
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Human monocytes tolerant to LPS retain the ability to phagocytose bacteria and generate reactive oxygen species BJMBR
Fernandes,M.L.; Mendes,M.E.; Brunialti,M.K.C.; Salomão,R..
Tolerance to lipopolysaccharide (LPS) occurs when animals or cells exposed to LPS become hyporesponsive to a subsequent challenge with LPS. This mechanism is believed to be involved in the down-regulation of cellular responses observed in septic patients. The aim of this investigation was to evaluate LPS-induced monocyte tolerance of healthy volunteers using whole blood. The detection of intracellular IL-6, bacterial phagocytosis and reactive oxygen species (ROS) was determined by flow cytometry, using anti-IL-6-PE, heat-killed Staphylococcus aureus stained with propidium iodide and 2',7'-dichlorofluorescein diacetate, respectively. Monocytes were gated in whole blood by combining FSC and SSC parameters and CD14-positive staining. The exposure to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: IL-6; ROS; Phagocytosis; MALP-2; Flagellin; LPS tolerance.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900008
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Hydrogen sulfide protects H9c2 cardiomyoblasts against H2O2-induced apoptosis BJMBR
Zhang,You En; Huang,Guang Qing; Wu,Bing; Lin,Xin Duo; Yang,Wen Zi; Ke,Zun Yu; Liu,Jie.
Reactive oxygen species (ROS) are highly reactive chemical species that may cause irreversible tissue damage, and play a critical role in cardiovascular diseases. Hydrogen sulfide (H2S) is a gasotransmitter that acts as a ROS scavenger with cardio-protective effects. In this study, we investigated the cytoprotective effect of H2S against H2O2-induced apoptosis in cardiomyocytes. H9c2 rat cardiomyoblasts were treated with H2S (100 μM) 24 h before challenging with H2O2 (100 μM). Apoptosis was then assessed by annexin V and PI, and mitochondrial membrane potential was measured using a fluorescent probe, JC-1. Our results revealed that H2S improved cell viability, reduced the apoptotic rate, and preserved mitochondrial membrane potential. An increased Bcl-2 to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Reactive oxygen species; ROS; Hydrogen sulfide; H2O2-induced apoptosis; Cardiomyocytes.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000400608
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Induction of apoptosis by zerumbone isolated from Zingiber zerumbet (L.) Smith in protozoan parasite Leishmania donovani due to oxidative stress BJID
Mukherjee,Debarati; Singh,Chingakham Brajakishor; Dey,Somaditya; Mandal,Supratim; Ghosh,Joydip; Mallick,Suvadip; Hussain,Aabid; Swapana,Ningombam; Ross,Samir Anis; Pal,Chiranjib.
Abstract In the present context of emergence of resistance aligned with the conventional anti-leishmanial drugs and occasional treatment failure compelled us to continue the search for replaceable therapeutic leads against Leishmaniainfection. Various ginger spices of the Zingiberaceae family are widely used as spices, flavouring agents, and medicines in Southeast Asia because of their unique flavour as well as due to their medicinal properties. Zerumbone, a natural component of Zingiber zerumbet (L.) Smith, has been studied for its pharmacological potential as antiulcer, antioxidant, anticancer, and antimicrobial. In this study, we have shown that zerumbone could induce ROS mediated apoptosis in Leishmania donovani promastigotes and also found effective...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leishmania donovani; Zingiber zerumbet; Zerumbone; Anti-leishmanial; ROS.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000100048
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Inhibitory effects on the production of inflammatory mediators and reactive oxygen species by Mori folium in lipopolysaccharide-stimulated macrophages and zebrafish Anais da ABC (AABC)
KWON,DA HYE; JEONG,JIN WOO; CHOI,EUN OK; LEE,HYE WON; LEE,KI WON; KIM,KI YOUNG; KIM,SUNG GOO; HONG,SU HYUN; KIM,GI-YOUNG; PARK,CHEOL; HWANG,HYE-JIN; SON,CHANG-GUE; CHOI,YUNG HYUN.
ABSTRACT Mori folium, the leaf of Morus alba L. (Moraceae), has been traditionally used for various medicinal purposes from ancient times to the present. In this study, we examined the effects of water extract of Mori folium (WEMF) on the production of inflammatory mediators, such as nitric oxide (NO) and prostaglandin E2 (PGE2), and reactive oxygen species (ROS) in lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophages. Our data indicated that WEMF significantly suppressed the secretion of NO and PGE2 in RAW 264.7 macrophages without any significant cytotoxicity. The protective effects were accompanied by a marked reduction in their regulatory gene expression at the transcription level. WEMF attenuated LPS-induced intracellular ROS production in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mori folium; Inflammation; ROS; Macrophage; Zebrafish.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000200661
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Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes Genet. Mol. Biol.
Djelic,Ninoslav; Djelic,Dijana; Spremo-Potparevic,Biljana; Zivkovic,Lada; Markovic,Biljana; Lozance,Olivera; Blagojevic,Milos.
Thyroid hormones stimulate aerobic metabolism which may lead to oxidative stress accompanied by damage to various cellular macromolecules, including DNA. Previous comet assay studies have shown that thyroid hormones cause DNA damage due to the creation of reactive oxygen species (ROS). However, cytogenetic studies have been equivocal because although an increase in the sister-chromatid exchange frequency per cell has been reported increased micronuclei frequency has not. We used cytogenetic examination of chromosome breakage and aberrations in whole-blood cultures of human peripheral blood lymphocytes to investigate possible clastogenic effects when lymphocytes were exposed to 0.002 µM to 50 µM of L-thyroxine for 24 h and 48 h, these concentrations being...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Thyroxine; Human lymphocytes; Chromosome aberrations; Mitotic index; ROS.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600019
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Multiple stresses on the oxidative metabolism of sugarcane varieties Ciência Rural
Morais,Marciana Bizerra de; Camara,Terezinha Rangel; Ulisses,Cláudia; Carvalho Filho,José Luiz Sandes; Willadino,Lilia.
ABSTRACT: The exposure of plants to multiple stress conditions can cause over production of reactive oxygen species promoting an imbalance in the redox system. The aim of this study was to examine the antioxidant response from varieties of sugarcane under adverse conditions, to understand the defense mechanisms of tolerant and sensitive genotypes. Micropropagated sugarcane of six genotypes, RB966928, RB98710, RB855453, RB99395, RB867515, and RB855156, were subjected to isolated action of high-temperature stress and a combination of water and saline stress, and effects were evaluated. Activities of enzymes such as superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX), as well as stress indicators such as lipid peroxidation (MDA content)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant enzyme; Abiotic stress; Saccharum; ROS.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000400201
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Protein carbonyl content: a novel biomarker for aging in HIV/AIDS patients BJID
Kolgiri,Vaishali; Patil,Vinayak Wamanrao.
Abstract Background: The major complications of “treated” Human Immunodeficiency Virus (HIV) infection are cardiovascular disease, malignancy, renal disease, liver disease, bone disease, and perhaps neurological complications, which are phenomena of the normal aging process occurring at an earlier age in the HIV-infected population. The present study is aimed to explore protein carbonyl content as a biomarker for detecting oxidative DNA damage induced ART toxicity and/or accelerated aging in HIV/AIDS patients. Objective: To investigate the potential of carbonyl content as a biomarker for detecting oxidative Deoxyribonucleic acid (DNA) damage induced Antiretroviral Theraphy (ART) toxicity and/or accelerated aging in HIV/AIDS patients. Methods: In this...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carbonyl content; DNA damage; Human immunodeficiency virus (HIV) infection; 8-OH-dG; ROS.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000100035
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Responses of the maize plant to chromium stress with reference to antioxidation activity Braz. J. Plant Physiol.
Maiti,Saborni; Ghosh,Nirmalia; Mandal,Chiranjib; Das,Kingsuk; Dey,Narottam; Adak,Malay Kumar.
Two maize varieties, viz. Sartaj and Deccan, have been evaluated according to their responses to chromium (Cr) in terms of specific physiological and biochemical attributes. During the exposure to Cr concentration (0, 50, 100, 200 and 300 µM) of potassium dichromate (K2Cr2O7) for seven days, significant changes of oxidative stress in terms of lipid peroxidation and protein oxidation were recorded along with other antioxidative pathways. It is revealed that irrespective of varieties, Sartaj was more prone to oxidative stress since the amount of superoxide due to Cr exposure was found to be significant, unlike the second variety, Deccan. In addition, for Sartaj the activity of NAD(P)H oxidase recorded a significant linear increase in a dose response...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidative enzymes; MDA; Protein oxidation; ROS.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202012000300007
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Selected spices and their combination modulate hypercholesterolemia-induced oxidative stress in experimental rats Biol. Res.
Otunola,Gloria A; Oloyede,Oyelola B; Oladiji,Adenike T; Afolayan,Anthony J.
BACKGROUND: Effect of aqueous extracts of Allium sativum (garlic), Zingiber officinale (ginger), Capsicum fructensces (cayenne pepper) and their mixture on oxidative stress in rats fed high Cholesterol/high fat diet was investigated. Rats were randomly distributed into six groups (n&#8201;=&#8201;6) and given different dietary/spice treatments. Group 1 standard rat chow (control), group 2, hypercholesterolemic diet plus water, and groups 3, 4, 5, 6, hypercholesterolemic diet with 0.5 ml 200 mg&#8201;·&#8201;kg-1 aqueous extracts of garlic, ginger, cayenne pepper or their mixture respectively daily for 4 weeks. RESULTS: Pronounced oxidative stress in the hypercholesterolemic rats evidenced by significant (p&#8201;<&#8201;0.05)...
Tipo: Journal article Palavras-chave: Lipid peroxidation; Oxidative stress; Spices; Hypercholesterolemia; ROS.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100005
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Study of the antioxidant capacity in gills of the Pacific oyster Crassostrea gigas in link with its reproductive investment ArchiMer
Beguel, Jean-philippe; Huvet, Arnaud; Quillien, Virgile; Lambert, Christophe; Fabioux, Caroline.
Energy allocation principle is a core element of life-history theory in which "the cost of reproduction" corresponds to an acceleration of senescence caused by an increase in reproductive investment. In the "theory of aging", senescence is mainly due to the degradation of lipids, proteins and DNA by reactive oxygen species (ROS), by-products of oxidative metabolism. Some studies have shown that oxidative stress susceptibility could be a cost of reproduction. The present study investigates the effect of reproductive investment on antioxidant capacity in the gills of a species with a very high reproductive investment, the Pacific oyster Crassostrea gigas. We used RNA interference targeting the oyster vasa-like gene (Oyvlg) to produce oysters with contrasted...
Tipo: Text Palavras-chave: Antioxidant capacity; Crassostrea gigas; Reproduction; RNA interference; ROS.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00116/22744/21494.pdf
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Synergy between cadmium and zinc in bean plants cultivated in multi contaminated soils Agronomy
Almeida, Lorena Gabriela; Silva, Dayane Meireles da; Jorge, Adelcio de Paula; Souza, Kamila Rezende Dázio de; Guilherme, Luiz Roberto Guimarães; Alves, José Donizeti.
Agricultural species are subjected to a variety of biotic and abiotic stresses, which are the main limitations to crop production. In this context, contamination by trace elements is characterized as an abiotic stress that represents an environmental problem. Due to the physical and chemical similarities between cadmium and zinc, these elements may interact in the environment and may cause antagonistic or synergistic effects. In this way, physiological mechanisms to exclude, detoxify or compartmentalize trace elements that are in excess are crucial for plant survival when exposed to high concentrations of these elements. In this way, the aim of this study was to understand the physiological responses of Phaseolus vulgaris plants subjected to increasing...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trace elements; Antioxidante enzymes; ROS; Photosynthesis; Synergism.; Fisiologia Vegetal.
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/35829
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Synergy between cadmium and zinc in bean plants cultivated in multi contaminated soils Agronomy
Almeida, Lorena Gabriela; Silva, Dayane Meireles da; Jorge, Adelcio de Paula; Souza, Kamila Rezende Dázio de; Guilherme, Luiz Roberto Guimarães; Alves, José Donizeti.
Agricultural species are subjected to a variety of biotic and abiotic stresses, which are the main limitations to crop production. In this context, contamination by trace elements is characterized as an abiotic stress that represents an environmental problem. Due to the physical and chemical similarities between cadmium and zinc, these elements may interact in the environment and may cause antagonistic or synergistic effects. In this way, physiological mechanisms to exclude, detoxify or compartmentalize trace elements that are in excess are crucial for plant survival when exposed to high concentrations of these elements. In this way, the aim of this study was to understand the physiological responses of Phaseolus vulgaris plants subjected to increasing...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trace elements; Antioxidante enzymes; ROS; Photosynthesis; Synergism. Fisiologia Vegetal.
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/35829
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