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Registros recuperados: 88
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A naturally occurring mutation K220T in the pleiotropic activator PrfA of Listeria monocytogenes results in a loss of virulence due to decreasing DNA-binding affinity Inra
Velge, P.; Herler, M.; Johansson, J.; Roche, S.; Témoin, S.; Fedorov, A. A.; Gracieux, P.; Almo, S. C.; Goebel, W.; Cossart, P..
The sequencing of prfA, encoding the transcriptional regulator of virulence genes, in 26 low virulence field Listeria monocytogenes strains showed that eight strains exhibited the same single amino-acid substitution: PrfAK220T. These strains exhibited no expression of PrfA-regulated proteins and thus no virulence. This substitution inactivated PrfA, since expression of the PrfAK220T mutant gene in an EGDΔprfA strain did not restore the haemolytic and phosphatidylcholine phospholipase C activities, in contrast to the wild-type prfA gene. The substitution of the lysine at position 220 occurred in the helix αH. However, the data showed that the PrfAK220T protein is dimerized just as well as its wild-type counterpart, but does not bind to PrfA boxes....
Tipo: Journal Article Palavras-chave: LISTERIA MONOCYTOGENES; PROTEINE; FACTEUR DE VIRULENCE; BACTERIE PATHOGENE; ADN; SEQUENCE NUCLEOTIDIQUE; REGULATION; TRANSCRIPTION; VIRULENCE; EXPRESSION DES GENES; ACIDE AMINE; MUTATION; RT-PCR; ELECTROPHORESE; ENZYME; ARN POLYMERASE.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009c1895b3c&uri=/notices/prodinra1/2009/11/
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A novel natural mutation in HC-Pro responsible for mild symptomatology of Zucchini yellow mosaic virus (ZYMV, Potyvirus) in cucurbits Inra
Desbiez, C.; Girard, M.; Lecoq, H..
A natural mild isolate of Zucchini yellow mosaic virus was found to contain a mutation in the helper component (HC-Pro) within a conserved motif, “CDNQLD”, located 12 residues downstream from the “FRNK” motif involved in symptom severity. Introducing the mutation in an infectious cDNA clone of ZYMV resulted in an almost complete absence of symptoms, although viral accumulation was only partially reduced. The FRNK(X)12CDNQLD sequence might be part of a larger motif that is conserved in potyviruses and plays a role in symptomatology and/or silencing inhibition
Tipo: Journal Article Palavras-chave: POTYVIRUS; ZUCCHINI YELLOW MOSAIC VIRUS; ZYMV; RT-PCR; ELISA TESTS; DISEASE RESISTANCE.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010efc00e54&uri=/notices/prodinra1/2010/11/
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A Rapid and Efficient Method for Purifying High Quality Total RNA from Peaches (Prunus persica) for Functional Genomics Analyses Biol. Res.
MEISEL,LEE; FONSECA,BEATRIZ; GONZÁLEZ,SUSANA; BAEZA-YATES,RICARDO; CAMBIAZO,VERONICA; CAMPOS,REINALDO; GONZALEZ,MAURICIO; ORELLANA,ARIEL; RETAMALES,JULIO; SILVA,HERMAN.
Prunus persica has been proposed as a genomic model for deciduous trees and the Rosaceae family. Optimized protocols for RNA isolation are necessary to further advance studies in this model species such that functional genomics analyses may be performed. Here we present an optimized protocol to rapidly and efficiently purify high quality total RNA from peach fruits (Prunus persica). Isolating high-quality RNA from fruit tissue is often difficult due to large quantities of polysaccharides and polyphenolic compounds that accumulate in this tissue and co-purify with the RNA. Here we demonstrate that a modified version of the method used to isolate RNA from pine trees and the woody plant Cinnamomun tenuipilum is ideal for isolating high quality RNA from the...
Tipo: Journal article Palavras-chave: CDNA library; Fruit; Fruit trees; Functional genomics; Peach; Polysaccharides; Prunus persica; RNA isolation; Rosaceae; RT-PCR.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100010
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Anaerobiosis and low specific growth rates enhance hemolysin BL production by Bacillus cereus F4430/73 Inra
Duport, C.; Thomassin, S.; Bourel, G.; Schmitt, P..
Bacillus cereus F4430/73 produced the highest levels of hemolysin BL (HBL) when grown under anaerobiosis in MOD medium. Anaerobic cells grown in a chemostat at low specific growth rate (0.1–0.2 h–1) expressed up to sevenfold more HBL than did cells held at a faster growth rate. At 0.2 h–1, the presence of 90 mM glucose resulted in inhibition of HBL production. Glucose was found to repress HBL induction at the mRNA level, indicating the potential involvement of catabolite repression in the regulation of HBL. Based on these data, it is suggested that growth rate could be an effector of catabolite regulation of HBL.
Tipo: Journal Article Palavras-chave: TAUX DE CROISSANCE; RT-PCR; CHEMOSTAT; TOXICITE ALIMENTAIRE; ANAEROBIOSE; ENTEROTOXINE; GLUCOSE; HEMOLYSINE.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0400031335104596&uri=/notices/prodinra1/2010/10/
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Análise da expressao gênica do VEGF (vascular Endothelial growth factor) em placentas bovinas de animais clonados, produzidos in vitro e in vivo, Pelo método de rt-pcr semiquantitativo Repositório Alice
LANDIM, JR, L. P.; FERREIRA, C. R.; PERECIN, F.; NICIURA, S. C. M.; YAMAZAKI, W.; MIGLINO, M. A.; GARCIA, J. M..
O sucesso da produção de embriões por fecundação in vitro ou transferência nuclear depende de uma variedade de fatores, dos quais a formação de uma incompleta vascularização placentária representa uma das principais causas de perda gestacional precoce. Neste trabalho, avaliou-se a expressão relativa de Fator de Crescimento Endotélio-vascular (VEGF), um potente agente vasculogênico, em placentas de bovinos clonados por transferência nuclear (NT), produzidos por fecundação in vitro (FIV), ou por monta natural (MN). Verificou-se um decréscimo altamente significativo de expressão de VEGF nas placentas dos animais produzidos in vitro, quando comparados aos animais MN (1,32a; 0,21b; 0,31b, respectivamente para MN, FIV e NT, p<0,05; teste de Duncan), sugerindo...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Análise de expressão génica; Semiquantitativo; Placentas Bovinas; VEGF; Clone; RT-PCR.
Ano: 2006 URL: http://www.alice.cnptia.embrapa.br/handle/doc/256248
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Analyse structurale et transcriptionnelle des rétrotransposons du génome de la vigne, Vitis vinifera L. Inra
Moisy, C..
Les variétés de vigne (Vitis vinifera L.) sont composées d’un ensemble de clones, tous issus de la même et unique plante propagée par multiplication végétative sur de longues périodes. Ce processus doit en principe assurer la transmission du patrimoine génétique de la plante mère à la plante fille, cependant des mutations spontanées peuvent apparaître et être, dans certains cas, à l’origine de nouveaux génotypes. Les éléments transposables, par la variabilité génétique que peut générer leur mobilité, sont une source potentielle de mutation. Dans un contexte général de compréhension des mécanismes biologiques qui participent à l’évolution des génomes au cours des cycles de multiplication végétative, l’objectif de la thèse est d’étudier la structure et la...
Tipo: Thesis Palavras-chave: TVV1; VINE-1; GRET1; DIVERSITE INTRA-VARIETALE; RETROTRANSPOSON; ELEMENTS MOBILES; STRESS; TRANSCRIPTION; RT-PCR.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009f41483eb&uri=/notices/prodinra1/2009/02/
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Anopheles gambiae PRS1 modulates plasmodium development at both midgut and salivary gland steps Inra
Chertemps, T.; Mitri, C.; Perrot, S.; Sautereau, J.; Jacques, J.-C.; Thiery, I.; Bourgouin, C.; Rosinski-Chupin, I..
Background: Invasion of the mosquito salivary glands by Plasmodium is a critical step for malaria transmission. From a SAGE analysis, we previously identified several genes whose expression in salivary glands was regulated coincident with sporozoite invasion of salivary glands. To get insights into the consequences of these salivary gland responses, here we have studied one of the genes, PRS1 (Plasmodium responsive salivary 1), whose expression was upregulated in infected glands, using immunolocalization and functional inactivation approaches. Methodology/Principal Findings: PRS1 belongs to a novel insect superfamily of genes encoding proteins with DM9 repeat motifs of uncharacterized function. We show that PRS1 is induced in response to Plasmodium, not...
Tipo: Journal Article Palavras-chave: PLASMODIUM; MOUSTIQUE; GLANDE SALIVAIRE; SALIVE; MALADIE INFECTIEUSE; PROTEINE; TRANSMISSION DE LA MALADIE; VECTEUR DE LA MALADIE; INTERACTION MOLECULAIRE; RT-PCR; SDS-PAGE; ARTHROPODA; HEXAPODA; EXPRESSION DES GENES; MICROSCOPIE CONFOCALE; INSECTE; PARASITOSE MALARIA PARASITE; MOLECULAR-INTERACTIONS; BERGHEI SPOROZOITES; MOSQUITO; INVASION; INFECTION; PROTEIN; VECTOR; CELLS; FALCIPARUM.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010f5c08798&uri=/notices/prodinra1/2010/11/
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Avaliação de técnicas biomoleculares em ovócitos e amostras de sangue de fêmeas infectadas com vírus da Artrite Encefalite Caprina. Repositório Alice
SILVA, P. A. F. da; SOUSA, S. D.; SOUSA, K. C. de; VERAS, A. K. A.; CAVALCANTE, F. R. A.; ANDRIOLI, A..
2012
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: CAE; Vírus da artrite encefalite caprina; CAEV; PCR; RT-PCR; Diagnóstico; Caprino; Cabra; Doença animal; Artrite Encefalite Caprina; Ovócito; Goats; Animal disease; Caprine arthritis-encephalitis.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/938408
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Biological characterization and complete nucleotide sequence of a Tunisian isolate of Moroccan watermelon mosaic virus Inra
Yakoubi, S.; Desbiez, C.; Fakhfakh, H.; Wipf-Scheibel, C.; Marrakchi, M.; Lecoq, H..
During a survey conducted in October 2005, cucurbit leaf samples showing virus-like symptoms were collected from the major cucurbit-growing areas in Tunisia. DAS-ELISA showed the presence of Moroccan watermelon mosaic virus (MWMV, Potyvirus), detected for the first time in Tunisia, in samples from the region of Cap Bon (Northern Tunisia). MWMV isolate TN05-76 (MWMV-Tn) was characterized biologically and its full-length genome sequence was established. MWMV-Tn was found to have biological properties similar to those reported for the MWMV type strain from Morocco. Phylogenetic analysis including the comparison of complete amino-acid sequences of 42 potyviruses confirmed that MWMV-Tn is related (65% amino-acid sequence identity) to Papaya ringspot virus...
Tipo: Journal Article Palavras-chave: TEST ELISA MOROCCAN WATERMELON MOSAIC VIRUS; POTYVIRUS; MWMV; APHID TRANSMISSION; RT-PCR.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009795590d8&uri=/notices/prodinra1/2010/10/
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Biological properties and relative fitness of inter-subgroup cucumber mosaic virus RNA 3 recombinants produced in vitro Inra
Pierrugues, O.; Guilbaud, L.; Fernandez-Delmond, I.; Fabre, F.; Tepfer, M.; Jacquemond, M..
In vitro reverse transcription of a mixture of total RNA from plants infected with the I17F or R strains of cucumber mosaic virus (CMV), representative of subgroups IA and II, respectively, results in viral cDNA populations including rare recombinant RNA 3 molecules, some of which also have point mutations. The biological properties of 17 recombinants in the capsid gene or the39 non-coding region of RNA 3 were evaluated when associated with I17F RNAs 1 and 2. Sixviruses displayed deficiencies (non-viability, deficiencies for movement and/or replication, delayed infection, loss of aphid transmissibility). Nine induced symptoms close to those of I17F-CMV ontobacco and pepper plants. All recombinants bearing the movement protein (MP) of R-CMVand part or most...
Tipo: Journal Article Palavras-chave: GENOME VIRAL; ARN VIRAL; PROPRIETE BIOLOGIQUE CUCUMBER MOSAIC VIRUS; GENETIC DIVERSITY; CMV; RECOMBINAISON GENETIQUE; RT-PCR.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008309b90b2&uri=/notices/prodinra1/2008/06/
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Canine distemper diagnosis by RT-PCR in dog samples from São Paulo State submitted to rabies laboratory diagnosis MV&Z
Macedo, Carla Isabel; Peixoto, Zélia Maria Pinheiro; Castilho, Juliana Galera; Oliveira, Rafael de Novaes; Rodrigues, Adriana Cândido; Achkar, Samira Maria.
Canine distemper (CD) is one of the most important infectious diseases in domestic dogs. In Brazil, CD is still the principal cause of mortality of dogs in some urban populations. Although different viral gene sequences have been identified for detecting the canine distemper virus (CDV), the N gene appears to be a better target for the amplification of specific fragments from all CDV strains. Using RT-PCR targeted to the CDV N gene, during the year 2014 we analyzed 190 central nervous system (CNS) samples of dogs from the state of São Paulo, Brazil, with symptoms suggestive of encephalitis and sent them to the Pasteur Institute for the diagnosis of rabies. Positivity for CD was over 50%, indicating that the disease remains an important cause of canine...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine Distemper; RT-PCR; Encephalitis; Diagnosis cinomose canina; RT-PCR; Encefalite; Diagnóstico.
Ano: 2016 URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/31032
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CARACTERIZACIÓN SEROLÓGICA Y MOLECULAR DE POTYVIRUS ASOCIADOS A LA VIROSIS DEL TOMATE DE ÁRBOL EN ANTIOQUIA (COLOMBIA) Acta biol.Colomb.
AYALA VÁSQUEZ,MARILUZ; GONZÁLEZ JAIMES,PAOLA; GUTIÉRREZ,PABLO ANDRÉS; COTES,JOSÉ MIGUEL; MARÍN MONTOYA,MAURICIO.
El tomate de árbol (Solanum betaceum) es una fruta andina altamente nutritiva, con propiedades organolépticas únicas para el procesamiento industrial y el mercado internacional. En Antioquia, Colombia, este cultivo presenta diferentes problemas fitosanitarios, siendo especialmente limitante la Virosis, causada por un complejo viral del que hacen parte entre otras, especies del género Potyvirus. En este trabajo se evaluaron mediante pruebas de ELISA los niveles de incidencia de este grupo viral en cultivos de siete zonas del departamento de Antioquia y se determinó la identidad taxonómica de dos de los potyvirus asociados al cultivo. Las pruebas serológicas muestran la presencia de potyvirus en seis de las siete zonas evaluadas, alcanzando niveles...
Tipo: Journal article Palavras-chave: ELISA; Potyvirus; PVY; RT-PCR; Tamarillo.
Ano: 2010 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2010000300011
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Characterisation of a new potyvirus species infecting meadow saffron (Colchicum autumnale) Inra
Poutaraud, A.; Desbiez, C.; Lemaire, O.; Lecoq, H.; Herrbach, E..
The alkaloids contained in Colchicum autumnale seeds are used in numerous medicines. Good quality seeds are difficult to obtain from this undomesticated plant. Therefore, a research program was set up aiming to cultivate C. autumnale in order to improve alkaloid contents and seed yields. In this context, a collection was established in 1999 by transplanting corms from twelve different locations in Eastern France. However, serious symptoms of necrosis and decay have appeared in this collection since 2001. Electron microscopic observations of plants showing symptoms revealed the presence of filamentous particles and pinwheel-like structures characteristic of the Potyviridae family. Leaves and corms from symptomatic plants were assayed with potyvirus-specific...
Tipo: Journal Article Palavras-chave: COLCHICUM AUTUMNALE; LILIACEAE; PLANTE TOXIQUE; POTYVIRUS; RT-PCR; MSBV; MEADOW SAFFRON BREAKING VIRUS; GENOME VIRAL; VIRUS DES VEGETAUX; ALCALOIDE; TEST ELISA; SEQUENCE NUCLEOTIDIQUE.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0400031229104456&uri=/notices/prodinra1/2010/10/
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Characterisation of oxidative stress-resistant mutants of Streptococcus thermophilus CNRZ368 Inra
Fernandez, A.; Thibessard, A.; Borges, F.; Gintz, B.; Decaris, B.; Leblond-Bourget, N..
During industrial processes, the dairy organism Streptococcus thermophilus is exposed to stress conditions. Its ability to survive and grow in an aerobic environment indicates that it must possess defensive mechanisms against reactive oxygen species. To identify the genes involved in oxidative stress defence, a collection of mutants was generated by random insertional mutagenesis and screened for menadione sensitivity and resistance. Results obtained for resistant clones allowed the identification of eight loci. The insertions affected genes whose homologues in other bacteria were previously identified as being involved in stress response (deoB, gst) or transcription regulation (rggC) and five ORFs of unknown function. The tolerance of the eight mutants...
Tipo: Journal Article Palavras-chave: RT-PCR; RESISTANCE; STREPTOCOCCUS THERMOPHILUS CNRZ368.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0400029230106062&uri=/notices/prodinra1/2010/10/
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Characterization of Citrus tristeza virus isolates from Colombia Rev. Protección Veg.
Morales,Juan; Orlando,Acosta; Tamayo,Pablo; Peñaranda,José.
Isolates of Citrus tristeza virus around the world differ widely in their biological properties. These properties may depend on strain mixtures within individual trees. Monoclonal Antibody 13 (MCA13) is an antibody that detects almost all the severe strains causing Quick Decline and Stem Pitting CTV syndromes. The objectives of this work were: I) to determine the percentage of CTV isolates reactive to the MCA13 antibody in each Colombian region evaluated and II) to analyze the genetic diversity of isolates from these regions. In almost all the regions evaluated, CTV MCA13 reactive isolates were identified; however, only one was found in Mompox area. The coat protein gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR) and analyzed...
Tipo: Journal article Palavras-chave: Virus cross-protection; Citrus tristeza disease; Citrus spp; RT-PCR; SSCP.
Ano: 2013 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522013000100006
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Cloprostenol administration in the first week postpartum reduces expression of oxytocin receptors in the endometrium in Holstein-Zebu cows. Repositório Alice
CAMARGOS, A.; WOHLRES-VIANA, S.; COSTA, I. F.; CAMARGO, L. S. de A.; FERREIRA, J. C.; RAMOS, A. A.; OBA, E..
ABSTRACT The present study investigated the hormonal profile and expression of prostaglandin F2? (PGF2?), oxytocin and estrogen receptors in uterine tissues of postpartum cows treated with cloprostenol. Twenty HolsteinZebu crossbred cows were treated with saline solution (treatment CONT) or cloprostenol (treatment CLO), both administered two and five days postpartum. Blood samples were collected on days two, seven, 14, 21 and 28 postpartum for progesterone, PGF2? metabolite (PGFM) and estradiol determination, and endometrial biopsy was performed in order to quantify the expression of oxytocin receptor (OXTR), prostaglandin F receptor (PTGFR) and estrogen receptor 1 (ERS1) genes. In the CLO treatment, expression of OXTR was reduced (P<0.05) but no...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: PTGFR; ESRI; Progesterone; Estradiol; RT-PCR.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1085645
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Comparação entre ELISA e RT-PCR na detecção de vírus em parreirais no Nordeste do Brasil. Repositório Alice
PIO-RIBEIRO, G.; ANDRADE, G. P.; CATARINO, A. D. M.; FAJARDO, T. V. M.; NICKEL, O.; BARBOSA, M. A. G.; TAVARES, S. C. C. de H.; LEAL, C. M..
2013
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: ELISA; RT-PCR; Comparação; Detecção; Região Nordeste; Brasil; Viticultura; Uva; Doença de planta; Vírus.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/979488
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COMPARACIÓN DEL LÍMITE DE DETECCIÓN DE MÉTODOS DE ANÁLISIS DE ÁCIDOS NUCLEICOS PARA EL VIROIDE DEL ENANISMO DEL LÚPULO (HSVd) EN CÍTRICOS Rev. Protección Veg.
Soto,Marianela; González,Lien; Peralta,Esther Lilia; Duran-Vila,Nuria.
Se determinó y comparó el límite de detección de la electroforesis secuencial en geles de poliacrilamida (sPAGE), hibridación de ácidos nucleicos (NASH) y transcripción reversa-reacción en cadena de la polimerasa (RT-PCR) para el diagnóstico del viroide del enanismo del lúpulo (HSVd) en cítricos, basados en el análisis de ácidos nucleicos de plantas de cidro y pepino como amplificadores biológicos. El límite de detección de la sPAGE fue mayor (5x10-3) en plantas de pepino que en plantas de cidro (5x10-2); sin embargo, este fue 50 veces menos sensible que el de la NASH (10-4). El límite de detección de la RT-PCR en pepino fue el mayor de todos los métodos analizados (10-6). Dicho valor se potenció cuando los productos de RT-PCR fueron detectados por NASH en...
Tipo: Journal article Palavras-chave: Viroide; HSVd; SPAGE; NASH; RT-PCR; Diagnóstico molecular.
Ano: 2007 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522007000200005
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Comparison of different methods for total RNA extraction from sclerotia of Rhizoctonia solani Electron. J. Biotechnol.
Shu,Canwei; Sun,Si; Chen,Jieling; Chen,Jianyi; Zhou,Erxun.
Background Rhizoctonia solani (teleomorph: Thanatephorus cucumeris) is one of the most important pathogens of rice (Oryza sativa L.) that causes severe yield losses in all rice-growing regions. Sclerotia, formed from the aggregation of hyphae, are important structures in the life cycles of R. solani and contain a large quantity of polysaccharides, lipids, proteins and pigments. In order to extract high-quality total RNA from the sclerotia of R. solani, five methods, including E.Z.N.A.&#8482; Fungal RNA Kit, sodium dodecyl sulfate (SDS)-sodium borate, SDS-polyvinylpyrrolidone (PVP), guanidinium thiocyanate (GTC) and modified Trizol, were compared in this study. Results The electrophoresis results showed that it failed to extract total RNA from the...
Tipo: Journal article Palavras-chave: Polyvinylpyrrolidone; Rhizoctonia solani; RT-PCR; Sclerotia; Sodium dodecyl sulfate; Total RNA extraction.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000100009
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Considerações estatísticas na análise de dados de expressão gênica gerados pela técnica de RT-qPCR. Infoteca-e
LOBO, A. M. B. O.; LOBO, R. N. B..
A transcriptase reversa (RT) seguida de quantificação utilizando a técnica da reação em cadeia da polimerase (qPCR) em tempo real ou, simplesmente reverse transcription-qPCR (RT-qPCR), é um método in vitro para amplificação enzimática de sequências definidas de DNA complementar obtido a partir do RNA. A técnica de RT-qPCR é uma das mais utilizadas em experimentos de quantificação da expressão gênica por ser considerado um dos métodos mais sensível e específico de quantificação gênica dos que outros utilizados para esta finalidade. No entanto, para obter resultados, pela técnica de RT-qPCR, que possam ser adequadamente analisados é necessário que o experimento tenha um desenho experimental com tratamentos, ensaios, unidade experimental e delineamento bem...
Tipo: Documentos (INFOTECA-E) Palavras-chave: RT-qPCR; Expressão gênica; Expressão gênica relativa; RT-PCR; Genética animal; Genética molecular; Gene expression; Genetic processes; Genetics.
Ano: 2014 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/1007398
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