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Devenir des virus entériques humains en milieu marin : Apport des VLPs (Virus Like Particles) pour la purification des coquillages ArchiMer
Loisy, Fabienne.
This work was developed following two research axes: the improvement of norovirus (human and animal strain) and rotavirus detection, and the evaluation of VLPs as a viral substitute for studying their comportment in marine environment. The main point concerning the method optimisation was the development of real-time RT-PCR for norovirus detection. The second part of the work has demonstrated the potential of norovirus and rotavirus VLPs as a viral surrogate as proven by the study of their stability in seawater. As VLPs are non-pathogen particles, the long persistence of rotavirus VLPs in shellfish has been shown in natural conditions (oysters on marine estuaries). The studies of Norwalk virus VLPs attachment to oyster tissues have demonstrated a specific...
Tipo: Text Palavras-chave: Specific binding; Persistence; Shellfish; Real time RT PCR; VLPs; Rotavirus; Norovirus; Fixation spécifique; Persistance; Coquillage; RT PCR en temps réel; VLPs; Rotavirus.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/these-327.pdf
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Quantitative relationship of two viruses (MrNV and XSV) in white-tail disease of Macrobrachium rosenbergii ArchiMer
Zhang, Hj; Wang, Hm; Yuan, Jf; Li, Lj; Zhang, Jh; Bonami, Jean-robert; Shi, Zl.
Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) were purified from diseased freshwater prawns M rosenbergii and used to infect healthy post-larvae (PL) by an immersion method. Three groups of prawns were challenged with various combined doses of MrNV and XSV. Signs of white-tail disease (WTD) were observed in Groups 1 and 2, which had been challenged with combinations containing relatively high proportions of MrNV and low proportions of XSV. By contrast there was little sign of WTD in Group 3, which had been challenged with a higher proportion of XSV than MrNV. A 2-step Taqman real-time RT-PCR was developed and applied to quantify viral copy numbers in each challenged PL. Results showed that genomic copies of both viruses were much...
Tipo: Text Palavras-chave: White tail disease; Real time RT PCR; Extra small virus; Nodavirus; Macrobrachium rosenbergii.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-3568.pdf
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