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Registros recuperados: 6
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Characterization and expression analysis of WOX2 homeodomain transcription factor in Aegilops tauschii Genet. Mol. Biol.
Zhao,Shan; Jiang,Qian-Tao; Ma,Jian; Wang,Ji-Rui; Liu,Ya-Xi; Chen,Guo-Yue; Qi,Peng-Fei; Pu,Zhi-En; Lu,Zhen-Xiang; Zheng,You-Liang; Wei,Yu-Ming.
The WUSCHEL (WUS)-related homeobox (WOX) gene family coordinates transcription during the early phases of embryogenesis. In this study, a putative WOX2 homolog was isolated and characterized from Aegilops tauschii, the donor of D genome of Triticum aestivum. The sequence consisted of 2045 bp, and contained an open reading frame (ORF), encoded 322 amino acids. The predicted protein sequence contained a highly conserved homeodomain and the WUS-box domain, which is present in some members of the WOX protein family. The full-length ORF was subcloned into prokaryotic expression vector pET-30a, and an approximately 34-kDa protein was expressed in Escherichia coli BL21 (DE3) cells with IPTG induction. The molecular mass of the expressed protein was identical to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: WOX2; Aegilops tauschii; Phylogenetic analysis; Real-time quantitative PCR.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100079
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Detection of human cytomegalovirus DNA in various blood components after liver transplantation BJMBR
Chen,X.Y.; Hou,P.F.; Bi,J.; Ying,C.M..
The quantification of human cytomegalovirus (HCMV DNA) by real-time PCR is currently a primary option for laboratory diagnosis of HCMV infection. However, the optimal sample material remains controversial due to the use of different PCR assays. To explore the best blood component for HCMV DNA surveillance after liver transplantation, whole blood (WB), serum (SE), and plasma (PL) specimens were collected simultaneously from targeted patients and examined for HCMV DNA using one commercially available assay. The HCMV DNA-positive rate with WB (16.67%) was higher than that with either SE or PL (8.33%, both P<0.01). Quantitative DNA levels in WB were of greater magnitude than those in SE (WB-SE mean log-transformed difference, 0.99; 95%CI=0.74-1.25;...
Palavras-chave: HCMV DNA; Blood components; Real-time quantitative PCR; Liver transplantation.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400340
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Different molecular mechanisms account for drought tolerance in Coffea canephora var. Conilon. Repositório Alice
VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; FREIRE, L. P.; VINECKY, F.; ELBELT, S.; SILVA, V. A.; MATTA, F. M. da; FERRAO, M. A. G.; MARRACCINI, P.; ANDRADE, A. C..
bitstream/item/179852/1/Vieira2013-Article-DifferentMolecularMechanismsAc.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Candidate gene; Real-time quantitative PCR; Coffea Canephora; Drought; Gene expression; Water potential.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/974852
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Differential expression of pathogenicity- and virulence-related genes of Xanthomonas axonopodis pv. citri under copper stress Genet. Mol. Biol.
Palmieri,Ana Carolina Basílio; Amaral,Alexandre Morais do; Homem,Rafael Augusto; Machado,Marcos Antonio.
In this study, we used real-time quantitative PCR (RT-qPCR) to evaluate the expression of 32 genes of Xanthomonas axonopodis pv. citri related to pathogenicity and virulence that are also involved in copper detoxification. Nearly all of the genes were up-regulated, including copA and copB. Two genes homologous to members of the type II secretion system (xcsH and xcsC) and two involved in the degradation of plant cell wall components (pglA and pel) were the most expressed in response to an elevated copper concentration. The type II secretion system (xcs operon) and a few homologues of proteins putatively secreted by this system showed enhanced expression when the bacteria were exposed to a high concentration of copper sulfate. The enhanced expression of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Copper resistance; Citrus canker; Real-time quantitative PCR.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200024
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Identification of chilling-responsive transcripts in peanut (Arachis hypogaea L.) Electron. J. Biotechnol.
Tang,Yue Yi; Wang,Chuan Tang; Yang,Guan Pin; Feng,Tong; Gao,Hua Yuan; Wang,Xiu Zhen; Chi,Xiao Yuan; Xu,Ya Long; Wu,Qi; Chen,Dian Xu.
To isolate differentially expressed peanut genes responsive to chilling, a suppression subtractive hybridization (SSH) cDNA library was constructed for a chilling tolerant peanut cultivar A4 with mRNAs extracted from the seeds imbibed at 2ºC and 15ºC, respectively, for 24 hrs. A total of 466 cDNA clones were sequenced, from which 193 unique transcripts (73 contigs and 120 singlets) were assembled. Of these unique transcripts, 132 (68.4%) were significantly similar to the sequences in GenBank non-redundant (nr) protein database, which belonged to diverse functional categories including metabolism, signal transduction, stress response, cell defense and transcriptional regulation. The remaining 61 (31.6%) showed no similarity to either hypothetical or known...
Tipo: Journal article Palavras-chave: Chilling response; Peanut; Real-time quantitative PCR; Suppression subtractive hybridization.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500005
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Molecular cloning and functional analysis of the fatty acid-binding protein (Sp-FABP) gene in the mud crab (Scylla paramamosain) Genet. Mol. Biol.
Zeng,Xianglan; Ye,Haihui; Yang,Ya'nan; Wang,Guizhong; Huang,Huiyang.
Intracellular fatty acid-binding proteins (FABPs) are multifunctional cytosolic lipid-binding proteins found in vertebrates and invertebrates. In this work, we used RACE to obtain a full-length cDNA of Sp-FABP from the mud crab Scylla paramamosain. The open reading frame of the full length cDNA (886 bp) encoded a 136 amino acid polypeptide that showed high homology with related genes from other species. Real-time quantitative PCR identified variable levels of Sp-FABP transcripts in epidermis, eyestalk, gill, heart, hemocytes, hepatopancreas, muscle, ovary, stomach and thoracic ganglia. In ovaries, Sp-FABP expression increased gradually from stage I to stage IV of development and decreased in stage V. Sp-FABP transcripts in the hepatopancreas and hemocytes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fatty acid-binding protein; Immunity; Ovary development; Real-time quantitative PCR; Scylla paramamosain.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100021
Registros recuperados: 6
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