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Registros recuperados: 54
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Análise da diversidade genética da população de Paenibacillus na rizosfera de milho plantado em solo de cerrado e de várzea, através de métodos moleculares. Repositório Alice
SILVA, K.R.; ROSADO, A.S.; MARRIEL, I. E.; PAIVA, E.; SELDIN, L..
1999
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Milho; Rizosfera; Cerrado; Varzea; Diversidade genetica; Zea mays; Paenibacillus; Maize; Rhizosphere; Genetic diversity; Savanna.
Ano: 1999 URL: http://www.alice.cnptia.embrapa.br/handle/doc/483538
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Avaliação do pH da rizosfera em dois genótipos de sorgo, em três saturações de alumínio e três níveis de potássio. Repositório Alice
PITTA, G.V.E.; BALIGAR, V.C.; CLARK, R.B.; RITCHIE, K.D.; ALVES, V. M. C.; VASCONCELLOS, C.A..
2000
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Sorgo; PH; Rizosfera; Aluminio; Solo acido; Potassio; Sorghum bicolor; Acid soils; Aluminium; Potassium; Rhizosphere.
Ano: 2000 URL: http://www.alice.cnptia.embrapa.br/handle/doc/484409
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Back to the roots: revealing beneficial interactions between the rhizosphere microbiome and wild wheat. Repositório Alice
ROSSMANN, M.; PÉREZ-JARAMILLO, J. E.; CHIARAMONTE, J. B.; KAVAMURA, V. N.; DUMACK, K.; FIORE-DONNO, A. M.; MENDES, L. W.; BONKOWSKI, M.; MAUCHLINE, T.; RAAIJMAKERS, J. M.; MENDES, R..
Domestication of plants may have impacted the interactions between plants and their rhizosphere microbiome. Considering that plants rely on the rhizosphere microbiome for functions related with plant growth, nutrition and health, the objective of this work was to analyse the rhizosphere microbiome of wild relatives and modern varieties of wheat and to investigate whether wild relatives recruit a higher proportion of beneficial microbes than modern cultivars. By 16S-rRNA gene amplicon, ITS and 18SrRNA gene amplicon, we assessed the bacterial, fungal and cercozoa rhizosphere community composition of five modern cultivars and three wheat ancestors cultivated in forest and agricultural soils. Results revealed a pronounced soil type and rhizosphere effect on...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Wild wheat; Trigo; Rizosfera; Rhizosphere; Wheat; Microbiome.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1103217
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Bacterial diversity associated with the rhizosphere of wheat plants (Triticum aestivum): Toward a metagenomic analysis Phyton
Velázquez-Sepúlveda,I; Orozco-Mosqueda,MC; Prieto-Barajas,CM; Santoyo,G.
Rhizospheric soil is one the largest reservoirs of microbial genetic diversity. Before conducting a large-scale metagenomic analysis of an environment, such as a rhizospheric soil, it is necessary to perform a pre-screening of the resident genetic diversity. In this study, we analyzed the bacterial diversity associated with the rhizosphere of wheat plants by PCR amplification, construction of a library and sequencing of 16S rDNA genes. Thirty OTUs were detected, including the Classes Alfaproteobacteria, Betaproteobacteria, Deltaproteobacteria, Gammaproteobateria, Actinobacteria, Bacilli, Clostridia and Uncultivable bacteria. Within the Gamma­proteobacteria class, the genera Pseudomonas, Stenotrophomonas and Bacillus were the most abundant, since they...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial diversity; Rhizosphere; Wheat; 16S ribosomal genes.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572012000100012
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Bacterial diversity in the rhizosphere of a transgenic versus a conventional maize (Zea mays) Phyton
Vital-López,L; Cruz-Hernández,MA; Fernández-Dávila,S; Mendoza-Herrera,A.
Genetically modified crops could cause negative effects on bacterial communities. In this study, we compared the bacterial community structure of two maize cultivars to determine whether the transgenic cultivar exerts a negative effect on bacterial communities inhabiting the rhizosphere. Cultivars included the genetically modified maize (Zea mays), with the pat-gene conferring resistance to the herbicide glufosinate (synonym: L-phosphinothricin), and the hybrid, conventional maize. Metagenomic DNA was extracted from the rhizosphere of plants grown in a greenhouse. Single-strand conformation polymorphism, based on polymerase chain reaction amplifying a partial subunit rRNA gene was used to characterize and generate genetic profiles that corresponded to the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zea mays; Glufosinate; Metagenomic DNA; Rhizosphere; Single-strand conformation polymorphism.
Ano: 2016 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572016000200006
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Biological nitrogen fixation and agronomic features of soybean (Glycine max (L.)Merr.) crop under different doses of inoculant Acta Agron. (Palmira)
Pedrozo,Alessandro; Girelli de Oliveira,Nelson João; Alberton,Odair.
Abstract Soybean is one of the most important crops in the world and presents great versatility. This study aims to investigate the effect of the Bradyrhizobium japonicum inoculant at 0, 600 and 1200 mL ha1 on soybean crop, with and without 1 L ha-1 calcium (Ca 41%) + 0.4 L ha-1 boron (B 14%). Plants were grown for 90 days in plastic pots with 3 kg of sand and vermiculite (v/v) autoclaved at 121oC for 1 h. The experiment was conducted in a completely randomized design with five replications in a greenhouse. Soybean yield parameters were evaluated, such as, dry weight of shoot, root and total; plant height, nitrogen in the shoot; number and dry weight of nodules; chlorophyll index in the leaves; number of pods per plant (NPPL); number of grains per pod...
Tipo: Journal article Palavras-chave: Bradyrhizobium; Inoculation; Plant-soil relations; Rhizosphere; Soil microbiology; Symbiosis.
Ano: 2018 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122018000200297
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Breeding for resistance to soil-borne pathogen impacts rhizosphere microbiome functions in common bean. Repositório Alice
MENDES, L. W.; MENDES, R.; RAAIJMAKERS, J. M.; TSAI, S. M..
Over the past century, plant breeding programs have substantially improved plant growth and health, but have not yet considered the potential effects on the plant microbiome. Here, we conducted a metatranscriptome analysis to determine if and how breeding for resistance of common bean against the root pathogen Fusarium oxysporum (Fox) affected gene expression in the rhizobacterial community. Our data revealed that rhizobacterial community assembly of Fox-resistant and susceptible bean cultivars follows niche-based mechanisms, presenting lower diversity and distinct community structure compared to the bulk soil. The microbiome of the Fox-resistant bean cultivar presented a significantly higher expression of bacterial genes associated with nutrient...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Rizosfera; Feijão; Microbiome; Rhizosphere; Beans; Breeding.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1103238
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Comparison of Paenibacillus azotofixans strains isolated from rhizoplane, rhizosphere, and non-root-associated soil from maize planted in two different Brazilian soils. Repositório Alice
SELDIN, L.; ROSADO, A. S.; CRUZ, D. W. da; NOBREGA, A.; ELSAS, J. D. van; PAIVA, E..
Paenibacillus azotofixans is a nitrogen-fixing bacterium often found in soil and in the rhizospheres of different grasses. In this study, two Brazilian clay soils were planted with cross-hybrid maize (BR-201) and four stages of plant growth were analysed to characterize the P. azotofixans populations present in the rhizoplanes, rhizospheres, and non-root-associated soils (herein called nonrhizospheres). A total of 106 strains were isolated and identified as P. azotofixans with an API 50CH kit, by classical biochemical tests, and via the use of specific primers based on the 16S rRNA gene in PCRs. To compare the isolated strains, phenotypic characteristics were determined and three different probes were used in hybridization experiments: two nif probes and...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Maize; Soil types; Cerrado soils; Clay soils; Rhizosphere.
Ano: 1998 URL: http://www.alice.cnptia.embrapa.br/handle/doc/489147
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Composition of arbuscular mycorrhizal fungi communities signals generalist species in soils cultivated with strawberry. Repositório Alice
CHIOMENTO, J. L. T.; STRÜRMER, S. L.; CARRENHO, R.; COSTA, R. C. da.; SCHEFFER-BASSO, S. M.; ANTUNES, L. E. C.; NIENOWA, A. A.; CALVETE, E. O..
bitstream/item/197053/1/Luis-Eduardo-Composition-of-arbuscular-mycorrhizal.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Fragaria X ananassaduch Duch; Diversity; Natural ecosystems; Agricultural ecosystems; Rhizosphere.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1108828
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Construction and characterization of a metagenomic DNA library from the rhizosphere of wheat (Triticum aestivum) Phyton
Hernández-León,R; Martínez-Trujillo,M; Valencia-Cantero,E; Santoyo,G.
Rhizospheric soil of wheat plants contains a high diversity of microorganisms, and therefore, comprises a large reservoir for discovering genes with diverse agro-biotechnological applications. In this work, we constructed an E. coli metagenomic library based on bacterial artificial chromosome (BAC) clones with large genomic inserts from metagenomic DNA from the rhizosphere of wheat plants. The average of the DNA cloned segments varies from 5 to 80 kb, with an average size of 38 kb. Random clones were end-sequenced and homology results showed that the clonation of metagenomic DNA codes mainly for metabolic and catalytic functions (40%), including amidohydrolase, hydrolase, peptidase, serine protease, endonuclease and exonuclease. Another interesting...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metagenomics; Rhizosphere; Wheat.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572012000100018
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Cross-kingdom similarities in microbiome ecology and biocontrol of pathogens. Repositório Alice
BERG, G.; KRAUSE, R.; MENDES, R..
Abstract: The concept of "gut and root microbiota commonalities" was already presented by Ramírez-Puebla et al.(2013); they discussed a lot of similar functional traits, host-bacteria interactions as well as evolutionary trends but also several differences. Based on deeper insights obtained by omics technologies, Mendes and Raaijmakers (2015) recently presented their concept that the structure and function of rhizosphere and gut microbiomes showcross-kingdom similarities. In parallel, Hacquard et al. (2015) analyzed similarities of the microbiota composition across plant and animal kingdoms and found only little overlap comparing fish gut and plant root communities. They explained the differences by various start inoculants and abiotic, niche-specific...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Ecological theories; Rizosfera; População microbiana; Bactéria; Controle biológico; Microbiome; Biological control agents; Rhizosphere; Pathogens.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1032834
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Disentangling the influence of earthworms in sugarcane rhizosphere. Repositório Alice
BRAGA, L. P. P.; YOSHIURA, C. A.; BORGES, C. D.; HORN, M. A.; BROWN, G. G.; DRAKE, H. L.; TSAI, S. M..
For the last 150 years many studies have shown the importance of earthworms for plant growth, but the exact mechanisms involved in the process are still poorly understood. Many important functions required for plant growth can be performed by soil microbes in the rhizosphere. To investigate earthworm influence on the rhizosphere microbial community, we performed a macrocosm experiment with and without Pontoscolex corethrurus (EW+ and EW?, respectively) and followed various soil and rhizosphere processes for 217 days with sugarcane. In EW+ treatments, N2O concentrations belowground (15 cm depth) and relative abundances of nitrous oxide genes (nosZ) were higher in bulk soil and rhizosphere, suggesting that soil microbes were able to consume earthworm-induced...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Crescimento de plantas; Minhoca; Solo; Rizosfera; Biologia do solo; Earthworms; Rhizosphere.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1067637
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Disponibilização de K de rochas silicáticas para plantas de soja inoculadas com fungos micorrízicos arbusculares (FMA). Infoteca-e
ANDRADE, L. R. M. de; FALEIRO, A. S. G.; SANTOS SOBRINHO, D. A.; NASCIMENTO, M. T. DO; SOUZA, A. L. DE.
2011
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Soja; Fungo micorrízico arbuscular; Rizosfera; Nutrientes; Soybeans; Arbuscular mycorrhizal fungus; Rhizosphere; Nutrients.
Ano: 2011 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/931541
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Diversidade metabólica de microrganismos em rizosfera de linhagens e híbridos de milho contrastantes para fósforo - campo. Repositório Alice
OLIVEIRA, C.A. de; MARRIEL, I. E.; ALVES, V. M. C.; PARENTONI, S. N.; PEREIRA FILHO, I. A.; SCHAFFERT, R. E.; MUZZI, M.R.S.; SA, N.M.H. de.
A avaliacao da diversidade microbiana e um bom indicador da qualidade dos solos e de diferencas existentes entre solos de rizosfera de plantas. O objetivo desse trabalho foi avaliar a diversidade metabolica de microrganismos totais da rizosfera, pelo sistema BIOLOG. O solos rizosferico de genotipos de milho, eficientes (E) e ineficientes (I) para fosforo, em alta e baixo P foram coletados na Embrapa Milho e Sorgo, Sete Lagoas, MG, Os hibridos avaliados foram: HT, HS1, HS2, eficientes e L22, ineficientes. Como amostras testemunhas: cerrado natural (mata), plantio direto e solo nao rizosferico dos hibridos e linhagens. As comunidades microbianas tivera, perfil distinto de utilizacao das fontes de carbono, conforme a analise de agrupamento e PCA. Amostras de...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Milho; Rizosfera; Diversidade; Microorganismos; Fosforo; Cerrado; Zea mays; Maize; Rhizosphere; Diversity; Microorganisms; Phosphorus; Savanna.
Ano: 2002 URL: http://www.alice.cnptia.embrapa.br/handle/doc/474378
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Diversidade microbiana na rizosfera de híbridos de milho contrastantes na eficiciência para fósforo sob condições controladas. Repositório Alice
BRASIL, E.C.; ALVES, V. M. C.; MARRIEL, I. E.; OLIVEIRA, C.A. de; CARVALHO, J.G. de.
Objetivando avaliar a diversidade microbiana da rizosfera de hibridos de milho contrastantes na eficiencia para P, conduziu-se um experimento com genotipos de milho combinados com niveis de P (baixo e alto). Os hibridos eficientes foram HS1, HS4 e HT1 e os ineficientes foram HS2 e HS3. A avaliacao da diversidade metabolica foi realizada em suspensao microbiana de rizosfera, utilizando o sistema BIOLOG Ecoplates. O desenvolvimento de cor, em termos de atividade total, do extrato microbiano da rizosfera de HS3 e HS4 foi superior aos demais. No entanto, o HS4 apresentou menor riqueza de substrato, indicando intensa atividade metabolica dos substratos utilizados. A atividade total, Riqueza e Equivalencia de substrato foram sempre elevados, em baixo-O,...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Milho; Rizosfera; Fosforo; Eficiencia; Zea mays; Maize; Rhizosphere; Phosphorus.
Ano: 2002 URL: http://www.alice.cnptia.embrapa.br/handle/doc/474268
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Efeito fungistático de Bacillus thuringiensis e de outras bactérias sobre alguns fungos fitopatogênicos. Repositório Alice
BATISTA JÚNIOR, C.B.; ALBINO, U.B.; MARTINES, A.M.; SARIDAKIS, D.P.; MATSUMOTO, L.S.; AVANZI, M.A.; ANDRADE, G..
Quatro isolados bacterianos da rizosfera de Drosera villosa var. villosa (B1, B2, B3, B4) e dois isolados de Bacillus thuringiensis (B5 e B6), sendo B6 produtor da toxina bioinseticida Cry1Ab, foram avaliados quanto à capacidade de inibir os fungos fitopatogênicos Fusarium solani f. sp. phaseoli, Fusarium solani f. sp. glycines, Fusarium oxysporum e Colletotrichum sp. A cepa mais efetiva foi B1 que inibiu o crescimento dos quatro fungos até o 26o dia. B. thuringiensis inibiu o crescimento de três destes, o que indica que possui atividade antifúngica e abre um novo campo de estudo para a utilização do B. thuringiensis.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Bactéria; Rizosfera; Controle biológico; Bacteria; Rhizosphere; Biological control.
Ano: 2002 URL: http://www.alice.cnptia.embrapa.br/handle/doc/108320
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Efeitos de fungos MVA no teor de proteína dos grãos no consórcio sorgo granífero - soja. Repositório Alice
BRESSAN, W.; SIQUEIRA, J.O.; VASCONCELLOS, C.A.; PURCINO, A. A. C..
1994
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Qualidade nutricional; Rizosfera; Sorgo granifero; Soja; Sorghum bicolor; Glycine max; Grain; Sorghum; Soybean; Nutritional; Quality; Rhizosphere.
Ano: 1994 URL: http://www.alice.cnptia.embrapa.br/handle/doc/478544
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Effect of Bacillus thuringiensis on microbial functional groups in sorghum rhizosphere. Repositório Alice
BRASIL, C.; MATSUMOTO, L.S.; NOGUEIRA, M.A.; SPAGO, F.R.; RAMPAZO, L.G.; CRUZ, M.F.; ANDRADE, G..
The objective of this work was to assess the effect of two strains of Bacillus thuringiensis var. kurstaki on sorghum rhizosphere microorganisms. The strains were HD1, that produces the bioinsecticidal protein, and 407, that is a mutant non-producer. The strains do not influence microbial population, but reduce plant growth and improve mycorrhizal colonization and free living fixing N2 community.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Bioinsecticide; Sorgum; Rhizosphere; Biocontrol; Bioinseticida; Sorgo; Rizosfera; Controle biológico.
Ano: 2006 URL: http://www.alice.cnptia.embrapa.br/handle/doc/108373
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Effect of plant photosynthesis, carbon sources and ammonium availability on nitrogen fixation rates in the rhizosphere of Zostera noltii ArchiMer
Welsh, Dt; Bourgues, S; Dewit, R; Auby, Isabelle.
Rates of nitrogen fixation (measured as acetylene reduction) in the rhizosphere of the seagrass Zostera noltii were highly dependent upon plant photosynthetic activity being significantly stimulated at elevated CO2 concentrations and by light, both in the short-term and over diurnal cycles. Stimulation by light became insignificant when 5 mM sucrose was added to the sediment porewater, indicating that in the absence of added carbon sources, light stimulation was due to direct inputs of plant photosynthate to the rhizosphere. Addition of a range of carbon sources to the rhizosphere sediment stimulated rates of acetylene reduction, with this stimulation being significant for sucrose and lactate. Surprisingly, whilst low additions of ammonium to the sediment...
Tipo: Text Palavras-chave: Acetylene reduction; Nitrogen fixation; Rhizosphere; Zostera noltii; Plant photosynthesis; Root exudates; Carbon sources; Ammonium.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00151/26266/24350.pdf
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Efficient protocols for the extraction of microbial DNA from the rhizosphere of hydrophilic forests in Chile Ciencia e Investigación Agraria
Cáceres,Pablo; Cordero,Cecilia; González,Gloria; Quiroz,Karla; Bobadilla,Juan C; Bravo,Carmen; Caligari,Peter D.S; Carrasco,Basilio; García-Gonzales,Rolando.
A lysis buffer-based protocol (Protocol BA), a modified lysis buffer-based protocol (Protocol BA Mod) and a commercial extraction kit (Protocol PS Kit) (Power Soil, Mo bio Laboratories, CA USA) were each evaluated for their ability to produce high-quality DNA with yields sufficient to allow its use in biodiversity studies. Similarly, the effect of liquid nitrogen on the process of cell disruption in all of the protocols that were studied. DNA yields ranged from 12.4 ng g-1 of processed soil to 9620 ng g-1 using the modified lysis buffer and commercial extraction kit, respectively. The quality of the DNA was determined by the ability of the DNA to produce efficient and reproducible polymerase chain reaction (PCR) products, using primers for universal 16S...
Tipo: Journal article Palavras-chave: Hydrophilic forests; 16S ribosomal RNA; PCR; Rhizosphere; Soil DNA extraction.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000300018
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