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| Registros recuperados: 54 | |
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| ROSSMANN, M.; PÉREZ-JARAMILLO, J. E.; CHIARAMONTE, J. B.; KAVAMURA, V. N.; DUMACK, K.; FIORE-DONNO, A. M.; MENDES, L. W.; BONKOWSKI, M.; MAUCHLINE, T.; RAAIJMAKERS, J. M.; MENDES, R.. |
| Domestication of plants may have impacted the interactions between plants and their rhizosphere microbiome. Considering that plants rely on the rhizosphere microbiome for functions related with plant growth, nutrition and health, the objective of this work was to analyse the rhizosphere microbiome of wild relatives and modern varieties of wheat and to investigate whether wild relatives recruit a higher proportion of beneficial microbes than modern cultivars. By 16S-rRNA gene amplicon, ITS and 18SrRNA gene amplicon, we assessed the bacterial, fungal and cercozoa rhizosphere community composition of five modern cultivars and three wheat ancestors cultivated in forest and agricultural soils. Results revealed a pronounced soil type and rhizosphere effect on... |
| Tipo: Resumo em anais de congresso (ALICE) |
Palavras-chave: Wild wheat; Trigo; Rizosfera; Rhizosphere; Wheat; Microbiome. |
| Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1103217 |
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| Velázquez-Sepúlveda,I; Orozco-Mosqueda,MC; Prieto-Barajas,CM; Santoyo,G. |
| Rhizospheric soil is one the largest reservoirs of microbial genetic diversity. Before conducting a large-scale metagenomic analysis of an environment, such as a rhizospheric soil, it is necessary to perform a pre-screening of the resident genetic diversity. In this study, we analyzed the bacterial diversity associated with the rhizosphere of wheat plants by PCR amplification, construction of a library and sequencing of 16S rDNA genes. Thirty OTUs were detected, including the Classes Alfaproteobacteria, Betaproteobacteria, Deltaproteobacteria, Gammaproteobateria, Actinobacteria, Bacilli, Clostridia and Uncultivable bacteria. Within the Gammaproteobacteria class, the genera Pseudomonas, Stenotrophomonas and Bacillus were the most abundant, since they... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial diversity; Rhizosphere; Wheat; 16S ribosomal genes. |
| Ano: 2012 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572012000100012 |
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| MENDES, L. W.; MENDES, R.; RAAIJMAKERS, J. M.; TSAI, S. M.. |
| Over the past century, plant breeding programs have substantially improved plant growth and health, but have not yet considered the potential effects on the plant microbiome. Here, we conducted a metatranscriptome analysis to determine if and how breeding for resistance of common bean against the root pathogen Fusarium oxysporum (Fox) affected gene expression in the rhizobacterial community. Our data revealed that rhizobacterial community assembly of Fox-resistant and susceptible bean cultivars follows niche-based mechanisms, presenting lower diversity and distinct community structure compared to the bulk soil. The microbiome of the Fox-resistant bean cultivar presented a significantly higher expression of bacterial genes associated with nutrient... |
| Tipo: Resumo em anais de congresso (ALICE) |
Palavras-chave: Rizosfera; Feijão; Microbiome; Rhizosphere; Beans; Breeding. |
| Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1103238 |
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| SELDIN, L.; ROSADO, A. S.; CRUZ, D. W. da; NOBREGA, A.; ELSAS, J. D. van; PAIVA, E.. |
| Paenibacillus azotofixans is a nitrogen-fixing bacterium often found in soil and in the rhizospheres of different grasses. In this study, two Brazilian clay soils were planted with cross-hybrid maize (BR-201) and four stages of plant growth were analysed to characterize the P. azotofixans populations present in the rhizoplanes, rhizospheres, and non-root-associated soils (herein called nonrhizospheres). A total of 106 strains were isolated and identified as P. azotofixans with an API 50CH kit, by classical biochemical tests, and via the use of specific primers based on the 16S rRNA gene in PCRs. To compare the isolated strains, phenotypic characteristics were determined and three different probes were used in hybridization experiments: two nif probes and... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Maize; Soil types; Cerrado soils; Clay soils; Rhizosphere. |
| Ano: 1998 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/489147 |
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| Hernández-León,R; Martínez-Trujillo,M; Valencia-Cantero,E; Santoyo,G. |
| Rhizospheric soil of wheat plants contains a high diversity of microorganisms, and therefore, comprises a large reservoir for discovering genes with diverse agro-biotechnological applications. In this work, we constructed an E. coli metagenomic library based on bacterial artificial chromosome (BAC) clones with large genomic inserts from metagenomic DNA from the rhizosphere of wheat plants. The average of the DNA cloned segments varies from 5 to 80 kb, with an average size of 38 kb. Random clones were end-sequenced and homology results showed that the clonation of metagenomic DNA codes mainly for metabolic and catalytic functions (40%), including amidohydrolase, hydrolase, peptidase, serine protease, endonuclease and exonuclease. Another interesting... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Metagenomics; Rhizosphere; Wheat. |
| Ano: 2012 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572012000100018 |
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| BRAGA, L. P. P.; YOSHIURA, C. A.; BORGES, C. D.; HORN, M. A.; BROWN, G. G.; DRAKE, H. L.; TSAI, S. M.. |
| For the last 150 years many studies have shown the importance of earthworms for plant growth, but the exact mechanisms involved in the process are still poorly understood. Many important functions required for plant growth can be performed by soil microbes in the rhizosphere. To investigate earthworm influence on the rhizosphere microbial community, we performed a macrocosm experiment with and without Pontoscolex corethrurus (EW+ and EW?, respectively) and followed various soil and rhizosphere processes for 217 days with sugarcane. In EW+ treatments, N2O concentrations belowground (15 cm depth) and relative abundances of nitrous oxide genes (nosZ) were higher in bulk soil and rhizosphere, suggesting that soil microbes were able to consume earthworm-induced... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Crescimento de plantas; Minhoca; Solo; Rizosfera; Biologia do solo; Earthworms; Rhizosphere. |
| Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/1067637 |
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| OLIVEIRA, C.A. de; MARRIEL, I. E.; ALVES, V. M. C.; PARENTONI, S. N.; PEREIRA FILHO, I. A.; SCHAFFERT, R. E.; MUZZI, M.R.S.; SA, N.M.H. de. |
| A avaliacao da diversidade microbiana e um bom indicador da qualidade dos solos e de diferencas existentes entre solos de rizosfera de plantas. O objetivo desse trabalho foi avaliar a diversidade metabolica de microrganismos totais da rizosfera, pelo sistema BIOLOG. O solos rizosferico de genotipos de milho, eficientes (E) e ineficientes (I) para fosforo, em alta e baixo P foram coletados na Embrapa Milho e Sorgo, Sete Lagoas, MG, Os hibridos avaliados foram: HT, HS1, HS2, eficientes e L22, ineficientes. Como amostras testemunhas: cerrado natural (mata), plantio direto e solo nao rizosferico dos hibridos e linhagens. As comunidades microbianas tivera, perfil distinto de utilizacao das fontes de carbono, conforme a analise de agrupamento e PCA. Amostras de... |
| Tipo: Artigo em anais de congresso (ALICE) |
Palavras-chave: Milho; Rizosfera; Diversidade; Microorganismos; Fosforo; Cerrado; Zea mays; Maize; Rhizosphere; Diversity; Microorganisms; Phosphorus; Savanna. |
| Ano: 2002 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/474378 |
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| BRASIL, E.C.; ALVES, V. M. C.; MARRIEL, I. E.; OLIVEIRA, C.A. de; CARVALHO, J.G. de. |
| Objetivando avaliar a diversidade microbiana da rizosfera de hibridos de milho contrastantes na eficiencia para P, conduziu-se um experimento com genotipos de milho combinados com niveis de P (baixo e alto). Os hibridos eficientes foram HS1, HS4 e HT1 e os ineficientes foram HS2 e HS3. A avaliacao da diversidade metabolica foi realizada em suspensao microbiana de rizosfera, utilizando o sistema BIOLOG Ecoplates. O desenvolvimento de cor, em termos de atividade total, do extrato microbiano da rizosfera de HS3 e HS4 foi superior aos demais. No entanto, o HS4 apresentou menor riqueza de substrato, indicando intensa atividade metabolica dos substratos utilizados. A atividade total, Riqueza e Equivalencia de substrato foram sempre elevados, em baixo-O,... |
| Tipo: Artigo em anais de congresso (ALICE) |
Palavras-chave: Milho; Rizosfera; Fosforo; Eficiencia; Zea mays; Maize; Rhizosphere; Phosphorus. |
| Ano: 2002 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/474268 |
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| BATISTA JÚNIOR, C.B.; ALBINO, U.B.; MARTINES, A.M.; SARIDAKIS, D.P.; MATSUMOTO, L.S.; AVANZI, M.A.; ANDRADE, G.. |
| Quatro isolados bacterianos da rizosfera de Drosera villosa var. villosa (B1, B2, B3, B4) e dois isolados de Bacillus thuringiensis (B5 e B6), sendo B6 produtor da toxina bioinseticida Cry1Ab, foram avaliados quanto à capacidade de inibir os fungos fitopatogênicos Fusarium solani f. sp. phaseoli, Fusarium solani f. sp. glycines, Fusarium oxysporum e Colletotrichum sp. A cepa mais efetiva foi B1 que inibiu o crescimento dos quatro fungos até o 26o dia. B. thuringiensis inibiu o crescimento de três destes, o que indica que possui atividade antifúngica e abre um novo campo de estudo para a utilização do B. thuringiensis. |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Bactéria; Rizosfera; Controle biológico; Bacteria; Rhizosphere; Biological control. |
| Ano: 2002 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/108320 |
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| BRASIL, C.; MATSUMOTO, L.S.; NOGUEIRA, M.A.; SPAGO, F.R.; RAMPAZO, L.G.; CRUZ, M.F.; ANDRADE, G.. |
| The objective of this work was to assess the effect of two strains of Bacillus thuringiensis var. kurstaki on sorghum rhizosphere microorganisms. The strains were HD1, that produces the bioinsecticidal protein, and 407, that is a mutant non-producer. The strains do not influence microbial population, but reduce plant growth and improve mycorrhizal colonization and free living fixing N2 community. |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Bioinsecticide; Sorgum; Rhizosphere; Biocontrol; Bioinseticida; Sorgo; Rizosfera; Controle biológico. |
| Ano: 2006 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/108373 |
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| Cáceres,Pablo; Cordero,Cecilia; González,Gloria; Quiroz,Karla; Bobadilla,Juan C; Bravo,Carmen; Caligari,Peter D.S; Carrasco,Basilio; García-Gonzales,Rolando. |
| A lysis buffer-based protocol (Protocol BA), a modified lysis buffer-based protocol (Protocol BA Mod) and a commercial extraction kit (Protocol PS Kit) (Power Soil, Mo bio Laboratories, CA USA) were each evaluated for their ability to produce high-quality DNA with yields sufficient to allow its use in biodiversity studies. Similarly, the effect of liquid nitrogen on the process of cell disruption in all of the protocols that were studied. DNA yields ranged from 12.4 ng g-1 of processed soil to 9620 ng g-1 using the modified lysis buffer and commercial extraction kit, respectively. The quality of the DNA was determined by the ability of the DNA to produce efficient and reproducible polymerase chain reaction (PCR) products, using primers for universal 16S... |
| Tipo: Journal article |
Palavras-chave: Hydrophilic forests; 16S ribosomal RNA; PCR; Rhizosphere; Soil DNA extraction. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000300018 |
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| Registros recuperados: 54 | |
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