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Registros recuperados: 14
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A large multi-pathogen waterborne community outbreak linked to faecal contamination of a groundwater system, France, 2000 ArchiMer
Gallay, A; De Valk, H; Cournot, M; Ladeuil, B; Hemery, C; Castor, C; Bon, F; Megraud, F; Le Cann, Pierre; Desenclos, Jc.
A large waterborne outbreak of infection that occurred during August 2000 in a local community in France was investigated initially via a rapid survey of visits to local physicians. A retrospective cohort study was then conducted on a random cluster sample of residents. Of 709 residents interviewed, 202 (28.5%) were definite cases (at least three liquid stools/day or vomiting) and 62 (8.7%) were probable cases (less than three liquid stools/day or abdominal pain). Those who had drunk tap water had a three-fold increased risk for illness (95% CI 2.4-4.0). The risk increased with the amount of water consumed (chi-square trend: p < 0.0001). Bacteriological analyses of stools were performed for 35 patients and virological analyses for 24 patients....
Tipo: Text Palavras-chave: Waterborne outbreak; Rotavirus; Norovirus; Gastroenteritis; Epidemiology; Campylobacter coli.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1696.pdf
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Bioinformatic prediction of polymerase elements in the rotavirus VP1 protein Biol. Res.
VÁSQUEZ-DEL CARPIÓ,RODRIGO; MORALES,JAIME L; BARRO,MARIO; RICARDO,ALBA; SPENCER,EUGENIO.
Rotaviruses are the major cause of acute gastroenteritis in infants world-wide. The genome consists of eleven double stranded RNA segments. The major segment encodes the structural protein VP1, the viral RNA-dependent RNA polymerase (RdRp), which is a minor component of the viral inner core. This study is a detailed bioinformatic assessment of the VP1 sequence. Using various methods we have identified canonical motifs within the VP1 sequence which correspond to motifs previously identified within RdRps of other positive strand, double-strand RNA viruses. The study also predicts an overall structural conservation in the middle region that may correspond to the palm subdomain and part of the fingers and thumb subdomains, which comprise the polymerase core of...
Tipo: Journal article Palavras-chave: Rotavirus; Genome replication; RNA polymerase; Bioinformatic analysis.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500008
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Characterization of mixed infections with different strains of bovine rotavirus in an outbreak of diarrhea in dairy herds in Goiás, Brazil BJM
Brito,Wilia Marta Elsner Diederichsen de; Munford,Veridiana; Villaça,André Martins; Caruzo,Thabata Alessandra Ramos; Rácz,Maria-Lucia.
Ten faecal samples of bovine rotavirus from calves less than 30 days old from an outbreak of diarrhea in Hidrolândia, Goiás, Brazil were submitted to serological and molecular characterization, using enzyme immunoassay for subgrouping and serotyping, PAGE for determination of electropherotypes and PCR for genome typing. Nine samples belonged to group A/subgroup I rotavirus and one sample was group A / subgroup non-I/non-II. Four samples were characterized as G10P[11] (B223-like), four samples showed a mixture of two rotavirus strains (G6G10 and P[5]P[11]), one sample was characterized as G6P[11] and one sample was characterized only by G serotyping/genotyping, and did not react with any P primer used. Two electropherotypes were detected and both were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rotavirus; Diarrhea; Bovine; Genotyping; Serotyping.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200015
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Contamination de l'environnement littoral par les rotavirus du groupe A. Détection par amplification enzymatique en chaîne et analyse du polymorphisme de restriction de séquences virales dans les eaux de surfaces et les coquillages ArchiMer
Dubois, Eric.
Les rotavirus font partie des principaux virus entériques humains susceptibles d'être rencontrés dans l'environnement. Leur mise en évidence par culture cellulaire dans des prélèvements est longue et peu sensible, ce qui explique le manque de données sur la contamination du littoral. La RT-seminested PCR a été développée pour pallier ces inconvénients. Après une mise au point méthodologique cette technique a été utilisée lors d'études in vitro et in situ. Les études in vitro sur le devenir des rotavirus en eau de mer, suivi par RT-PCR quantitative et par multiplication en culture cellulaire, montrent que dans des conditions proches de celles du milieu naturel (eau de mer non stérile et à une température relativement basse), la détection d'ARN viral peut...
Tipo: Text Palavras-chave: Rotavirus; Contamination; Coquillages; Eaux de stations d'épuration; Echantillons cliniques; RT-PCR; RFLP; Epidémiologie moléculaire.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/00442/55376/56894.pdf
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Devenir des virus entériques humains en milieu marin : Apport des VLPs (Virus Like Particles) pour la purification des coquillages ArchiMer
Loisy, Fabienne.
This work was developed following two research axes: the improvement of norovirus (human and animal strain) and rotavirus detection, and the evaluation of VLPs as a viral substitute for studying their comportment in marine environment. The main point concerning the method optimisation was the development of real-time RT-PCR for norovirus detection. The second part of the work has demonstrated the potential of norovirus and rotavirus VLPs as a viral surrogate as proven by the study of their stability in seawater. As VLPs are non-pathogen particles, the long persistence of rotavirus VLPs in shellfish has been shown in natural conditions (oysters on marine estuaries). The studies of Norwalk virus VLPs attachment to oyster tissues have demonstrated a specific...
Tipo: Text Palavras-chave: Specific binding; Persistence; Shellfish; Real time RT PCR; VLPs; Rotavirus; Norovirus; Fixation spécifique; Persistance; Coquillage; RT PCR en temps réel; VLPs; Rotavirus.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/these-327.pdf
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Genotipificación de Rotavirus en niños menores de cinco años en la región sur del Ecuador ABCL
Simaluiza-Masabanda,Rosa Janneth; Cevallos-Solórzano,Gabriela; Bourdett-Stanziola,Lurys; Arévalo-Jaramillo,Paulina; Ruiz-Ramón,Jeimmy; Villacis-Villa,Diana; Torres-Bailón,Paula C; Fernández Jaramillo,Heriberto.
Fueron estudiadas, para la búsqueda de rotavirus, 341 muestras fecales obtenidas de sendos niños menores de cinco años con diarrea aguda, atendidos en dos centros de salud de la ciudad de Loja - Ecuador. De ellas, 56 (16%) fueron positivas para rotavirus, siendo genotipificadas 33 muestras para la determinación de genotipos G y P. Los genotipos más frecuentes fueron G4 (42%), P[6] (36%) y la combinación mixta G4+9P[6] (21%). Este es el primer estudio de carácter molecular en rotavirus realizado en el sur de Ecuador y los datos obtenidos ratifican la variabilidad de cepas de rotavirus circulantes en este país.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rotavirus; Genotipos; Ecuador.
Ano: 2016 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572016000400015
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Monitoring and molecular characterization of group D rotavirus in Brazilian poultry farms. Repositório Alice
BESERRA, L. A. R.; BERNARDES, N. T. C. G.; BRANDÃO, P. E.; GREGORI, F..
Rotaviruses are etiological agents of diarrhea both in humans and in several animal species. Data on avian Group D rotaviruses (RVD) are scarce, especially in Brazil. We detected RVD in 4 pools of intestinal contents of broilers, layer and broiler breeders out of a total of 111 pools from 8 Brazilian states, representing an occurrence of 3.6%, by a specific RVD RT-PCR targeting the VP6 gene. Phylogenetic tree confirmed that the Brazilian strains belong to group D and 3 of the sequences were identical in terms of amino acid whereas one showed 99.5% identity with the others. The sequences described in this study are similar to other sequences previously detected in Brazil, confirming the conserved nature of the VP6 protein.
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Broilers; Ave; Frango; Rotavirus; Rotavirus; Broiler chickens.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1042215
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Occurrence of a single type strain (G3, P[12]) of equine rotavirus in foals on a racehorse farm from 1986 to 1993 OAK
Sakawaki, Hiromi; Ishizaki, Hiroshi; Takagi, Michihiro; Masuda, Gen; Taniguchi, Koki; Urasawa, Tomoko; Urasawa, Shozou; Goto, Hitoshi; Shirahata, Toshikazu; 阪脇, 廣美; 石崎, 宏; 高木, 道浩; 増田, 玄; 谷口, 孝喜; 浦沢, 价子; 浦沢, 正三; 後藤, 仁; 白幡, 敏一.
Palavras-chave: Foals; Persistence; Rotavirus.
Ano: 1997 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1325
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Possible relation between a winter epidemic of acute gastroenteritis in France and viral contamination of shellfish ArchiMer
Miossec, Laurence; Le Guyader, Soizick; Haugarreau, Larissa; Comps, Marie-annick; Pommepuy, Monique.
Several outbreaks of gastroenteritis related to the consumption of shellfish (frequently eaten raw) have been reported in different parts of the world. In Europe, hum an calicivirus infections may have been involved in winter outbreaks in recent years, although there is little evidence confirming such viral contamination in shellfish. This study presents the first results of a field survey on viral contamination in two shelllish harvesting areas along the French Mediterranean coast. The first, consisting mainly of oyster beds, was c1assified in category A, as determined by fecal coliform counts in shellfish (European Community Directive 91/492), and the second, a mussel bed, was c1assified in category C. Shelllish samples were collected monthly between...
Tipo: Text Palavras-chave: Shellfish; Viral contamination; Enterovirus; Human calicivirus; Rotavirus; Astrovirus; Fecal coliforms; Gastroenteritis.
Ano: 1998 URL: https://archimer.ifremer.fr/doc/00001/11257/7802.pdf
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Rotavirus and reovirus interaction with mouse peritoneal resident phagocytic cells BJMBR
Guimarães,M.A.A.M.; Nozawa,C.M.; Guimarães,A.C.C.; Ramos,S..
Rotaviruses and reoviruses are involved in human and animal diseases. It is known that both viruses penetrate the gastrointestinal tract but their interaction with phagocytic cells is unknown. To study this interaction, peritoneal resident phagocytic cells were used and rotavirus and reovirus replication in peritoneal phagocytic cells was observed. However, rotavirus replication in these cells led to the production of defective particles since MA-104 cells inoculated with rotavirus phagocytic cell lysate did not show any evidence of virus replication. On the basis of these results, we suggest that, although reovirus dissemination may be helped by these phagocytic cells, these cells may control rotavirus infection and probably contribute to the prevention...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rotavirus; Reovirus; Phagocytic cells.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000008
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Rotavirus VLP2/6: a new tool for tracking rotavirus in the marine environment ArchiMer
Loisy, Fabienne; Atmar, Robert L.; Cohen, Jean; Bosch, Albert; Le Guyader, Soizick.
The potential of rotavirus 2/6-virus-like-particles (VLP2/6) for use as tracers in the marine environment was investigated. The stability of bovine rotavirus (strain RF) and VLP2/6 in natural seawater at 25degreesC for six days was studied. ELISA and western blot methods were used to quantify the particles. The rates of decline of rotavirus particles and VLP2/6 were similar (approximately 0.5 log 10 per day). Western blot analysis showed that the integrity of capsid proteins VP2 and VP6 was conserved during the incubation time. These results demonstrate that VLP2/6 particles have the same stability in seawater as rotavirus particles. Thus, VLP2/6 can be used as a tracer, which should be of particular value for studying the fate of rotavirus particles in...
Tipo: Text Palavras-chave: Stability; Tracer; Seawater; Virus like particles; Rotavirus.
Ano: 2004 URL: https://archimer.ifremer.fr/doc/2004/publication-330.pdf
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The infectivity of pig rotavirus in stools Braz. J. Vet. Res. Anim. Sci.
RAMOS,Ana Paula Dores; STEFANELLI,Carla Cristina; LINHARES,Rosa Elisa Carvalho; BRITO,Benito Guimarães de; NOZAWA,Carlos Mitihiko.
Rotaviruses are the major pathogen for both human and animal. They affect young animals in intensive rearing and cause great economic losses. This study intended to evaluate the infectivity of porcine rotavirus maintained for 32 months at approximately 10ºC. in the original stools specimens. Thirty stools specimens of 1-4-week-old piglets originating from breeding farms located in the southwest region of the Paraná State were selected for this study. They were randomly chosen from stools samples positive for rotavirus by polyacrylamide gel electrophoresis (PAGE) at the time of collection. The thirty stools samples maintained for 32 months at approximately 10ºC were re-tested by PAGE and 11 out of 30 were still positive showing the integrity of the eleven...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rotavirus; Rotavirus Infection; Diarrhea; Swine.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961998000200007
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Viral contamination and depuration of oysters under field conditions ArchiMer
Boher, S; Piclet, Guy; Beril-stien, S; Masson, Daniel; Delonce, R; Biziagos, E; Schwartzbrod, L.
Shellfish consumption is clearly associated with the transmission of enteric viruses. The channel of transmission of enteric viruses to human beings could be decreased particularly if the systems of depuration of shellfish were optimized, standardized and if their viral efficiency was controlled. The purpose of this work was to study the contamination of oysters and to assess the efficiency of several depuration processes. Our study have demonstrated that in some cases, 50% of oysters samples contain enteric viruses or H.A.V. antigen and the quantities of viruses range from 17 to 246 FF for the rotaviruses and from 124 to 200 for the enteroviruses per 100 g of tissue. According to our results, the exclusive use of a bacteriological criterium to determine...
Tipo: Text Palavras-chave: Rotavirus; Hepatitis A virus; Enterovirus; Crassostrea gigas; Bivalvia; Seafood; Bioassays; Cell culture; Viruses; Self purification; Viral diseases; Shellfish.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-1615.pdf
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Virus Type-Specific Removal in a Full-Scale Membrane Bioreactor Treatment Process ArchiMer
Miura, Takayuki; Schaeffer, Julien; Le Saux, Jean-claude; Le Mehaute, Philippe; Le Guyader, Soizick.
We investigated removal of noroviruses, sapoviruses, and rotaviruses in a full-scale membrane bioreactor (MBR) plant by monitoring virus concentrations in wastewater samples during two gastroenteritis seasons and evaluating the adsorption of viruses to mixed liquor suspended solids (MLSS). Sapoviruses and rotaviruses were detected in 25% of MBR effluent samples with log reduction values of 3- and 2-logs in geometric mean concentrations, respectively, while noroviruses were detected in only 6% of the samples. We found that norovirus and sapovirus concentrations in the solid phase of mixed liquor samples were significantly higher than in the liquid phase (P < 0.01, t test), while the concentration of rotaviruses was similar in both phases. The efficiency...
Tipo: Text Palavras-chave: Norovirus; Sapovirus; Rotavirus; MBR; Mixed liquor suspended solids; Adsorption.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00414/52548/53441.pdf
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