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Adaptação de método de extração e caracterização de proteínas em bactéria de importância para a indústria alimentícia. Infoteca-e
STEPHAN, M. P.; TEIXEIRA, K. R. dos S..
bitstream/item/164076/1/CT113-extracao-proteinas.pdf
Tipo: Comunicado Técnico (INFOTECA-E) Palavras-chave: SDS-PAGE; Método de extração.; Eletroforese; Proteína..
Ano: 2007 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/413478
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Anopheles gambiae PRS1 modulates plasmodium development at both midgut and salivary gland steps Inra
Chertemps, T.; Mitri, C.; Perrot, S.; Sautereau, J.; Jacques, J.-C.; Thiery, I.; Bourgouin, C.; Rosinski-Chupin, I..
Background: Invasion of the mosquito salivary glands by Plasmodium is a critical step for malaria transmission. From a SAGE analysis, we previously identified several genes whose expression in salivary glands was regulated coincident with sporozoite invasion of salivary glands. To get insights into the consequences of these salivary gland responses, here we have studied one of the genes, PRS1 (Plasmodium responsive salivary 1), whose expression was upregulated in infected glands, using immunolocalization and functional inactivation approaches. Methodology/Principal Findings: PRS1 belongs to a novel insect superfamily of genes encoding proteins with DM9 repeat motifs of uncharacterized function. We show that PRS1 is induced in response to Plasmodium, not...
Tipo: Journal Article Palavras-chave: PLASMODIUM; MOUSTIQUE; GLANDE SALIVAIRE; SALIVE; MALADIE INFECTIEUSE; PROTEINE; TRANSMISSION DE LA MALADIE; VECTEUR DE LA MALADIE; INTERACTION MOLECULAIRE; RT-PCR; SDS-PAGE; ARTHROPODA; HEXAPODA; EXPRESSION DES GENES; MICROSCOPIE CONFOCALE; INSECTE; PARASITOSE MALARIA PARASITE; MOLECULAR-INTERACTIONS; BERGHEI SPOROZOITES; MOSQUITO; INVASION; INFECTION; PROTEIN; VECTOR; CELLS; FALCIPARUM.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010f5c08798&uri=/notices/prodinra1/2010/11/
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Assessment of genetic diversity among Pakistani wheat (Triticum aestivum L.) advanced breeding lines using RAPD and SDS-PAGE Electron. J. Biotechnol.
Ahmed,Muhammad Farooq; Iqbal,Muhammad; Masood,Muhammad Shahid; Rabbani,Malik Ashiq; Munir,Muhammad.
Genetic diversity was assessed among 32 advanced wheat breeding lines included in the National Uniform Wheat Yield Trials (2006-07) of Pakistan using molecular (DNA) and biochemical (SDS-PAGE) markers. Of the 72 RAPD primers used for initial screening, 15 were found polymorphic. A total of 140 bands (61.4% polymorphic) were generated by the 15 random decamer primers. Genetic similarity coefficients ranged from 0.81 to 0.94 for rainfed and from 0.70 to 0.93 for the normal seeding date group. Cluster analysis using the unweighted pair group method of arithmetic averages (UPGMA) clustered the 32 advanced wheat breeding lines into one major and three small groups. Maximum level of polymorphism (90%) was observed for the primer OPA-05. Lines N9 and N11 showed...
Tipo: Journal article Palavras-chave: Genetic diversity; Molecular markers; Polymorphism; RAPD; SDS-PAGE; Wheat.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300001
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Caracterización de las inmunoglobulinas del ñandú (Rhea americana) InVet
Montagna,D.R.; Rigo,V; Ramayo,L.G.; Goldman,L.H.; Huguet,M.J.; Maceira,N; Jar,A.M..
El objetivo de este trabajo fue caracterizar las inmunoglobulinas séricas y la IgY de la yema de huevo de ñandú, a través de las técnicas de electroforesis en acetato de celulosa, electroforesis en gel de poliacrilamida y Western-blot. Se estudiaron 19 ñandúes clínicamente sanos de 14 a 15 meses de edad, provenientes un criadero de Balcarce, Argentina. La electroforesis en acetato de celulosa de los sueros mostró la presencia de cuatro fracciones proteicas: albúmina, alfa, beta y gamma globulinas. La concentración de las gamma globulinas fue de 0,69 ± 0,18 g/dl, con un rango de 0,42-1,05 g/dl. La corrida electroforética de la IgY muestró que esta se ubica en la fracción gammaglobulínica. La electroforesis en gel de poliacrilamida evidenció, tanto en suero...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ã‘andú; Electroforesis; SDS-PAGE.
Ano: 2013 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982013000200002
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Characterization of cry2-type genes of Bacillus thuringiensis strains from soil-isolated of Sichuan basin, China BJM
Liang,Hongxia; Liu,Yao; Zhu,Jun; Guan,Peng; Li,Shuangcheng; Wang,Shiquan; Zheng,Aiping; Liu,Huainian; Li,Ping.
Sichuan basin, situated in the west of China, is the fourth biggest basin in China. In order to describe a systematic study of the cry2-type genes resources from Bacillus thuringiensis strains of Sichuan basin, a total of 791 Bacillus thuringiensis strains have been screened from 2650 soil samples in different ecological regions. The method of PCR-restriction fragment length polymorphism (PCR-RFLP) was used to identify the type of cry2 genes. The results showed that 322 Bacillus thuringiensis strains harbored cry2-type genes and four different RFLP patterns were found. The combination of cry2Aa/cry2Ab genes was the most frequent (90.4%), followed by cry2Aa (6.8%) and cry2Ab alone (2.5%), and only one novel type of cry2 gene was cloned from one isolate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus thuringiensis; PCR-RFLP; SDS-PAGE; Novel cry2-type gene.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100018
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Characterization of onion (Allium cepa L.) varieties based on physical properties and seed performance Agrociencia
Herrera-Corredor,Carlos; Carrillo-Castañeda,Guillermo.
Abstract: Five cultivars (Cristal White Wax, Toro, Red Burgundy, Cojumatlán and Contessa) of onion (Allium cepa L.) were characterized according to: the weight of 100 seeds, germination kinetics, speed germination (T50) and seed protein analysis. Seed of Crystal White Wax was differentiated from the other cultivars by its low weight (0.30 g), since the weight of the rest was 0.40 g. Crystal White Wax had low seed germination (56%) and the highest delay of germination (T50 values were 78 h), while T50 of the rest was 52 h. Protein was isolated from seeds at the precise moment of seed germination. All cultivars were differentiated by their pattern and number of proteins resolved by SDS-PAGE electrophoresis. Toro and Red Burgundy showed the greater difference...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allium cepa L.; Germination kinetics; SDS-PAGE; T50; Vigor.
Ano: 2007 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952007000700755
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Comparative electrophoretic profile of proteins and esterases in healthy silkworm larvae (Bombyx mori Lineu, 1758) and infected with nucleopolyhedrovirus - doi: 10.4025/actascibiolsci.v35i1.8612 Biological Sciences
Ruiz, Juliana Bueno; Universidade Estadual de Maringá; Ronqui, Ludimilla; Universidade Federal de Rondonia; Oliveira, Jussara Ricardo de; Universidade Paranaense; Ruvolo-Takasusuki, Maria Claudia Colla; Universidade Estadual de Maringá.
Total proteins and esterases from silk gland extracts of Bombyx mori silkworm were characterized and compared with electrophoretic profiles of prepared extracts with silkworm glands infected with nucleopolyhedrovirus (BmNPV). SDS-PAGE (7%) gels was used for the total proteins, and 10% native PAGE for esterases. In the silk glands extracts of healthy silkworms, it was observed seven protein zones with molecular weight varying between 10 kDa (P1) and 60 kDa (P7). In the infected silkworms, a new zone named P8 (90 kDa) was also detected. Esterases activity at 5th instar larvae underwent changes after the infection with BmNPV, since there was a reduction (EST-6 and EST-7) and an increase (EST-8) in the intensity of the regions of esterases activity, and...
Tipo: Genética Bioquímica Palavras-chave: 2.02.04.00-0 viral infection; Silk gland; Isoenzymes; SDS-PAGE; Molecular markers 2.02.02.00-8.
Ano: 2012 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/8612
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Comparison of electrophoretic protein profiles of Campylobacter jejuni subsp. jejuni isolated from different animal species BJM
Scarcelli,Eliana; Costa,Elizabeth Oliveira da; Genovez,Margareth Élide; Cardoso,Maristela Vasconcellos; Bach,Erna Elizabeth; Torres,Ana Paula.
Electrophoretic protein profiles of Campylobacter jejuni subsp. jejuni strains isolated from feces of seven animal species, including man, were compared. Fourteen strains (two from each species) plus two human strains and the reference one, were ruptured by ultrasound and their total soluble proteins were analyzed by SDS-PAGE technique in a 12% polyacrylamide gel with computerized densitometric reading by the molecular analyst software. All the strains had bands in common that correspond to 45 and 66 Kda molecular weight. The disagreement corresponded to a 97 to 200 Kda molecular weight region. From the 17 strains, 13 (76.5%), were classified as biotype I, three (17.6%) as biotype II and one (5.8%) as biotype III. Since protein extracts were obtained from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Campylobacter jejuni; SDS-PAGE; Biotyping.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400006
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Comparison of protein profile of co-existing Fasciola hepatica and Fasciola gigantica parasite in Bos taurus (cattle) and Bubalus bubalis (Philippine water buffalo) OAK
De Vera, M.E; Sato, K; Oyong, G; Claveria, F.G.
Fasciola hepatica and Fasciola gigantica habitually co-exist as parasites of cattle (Bos taurus) and water buffalo (Bubalus bubalis), and despite variation in their morphometry, their species status is suspicious. Liver flukes isolated from cattle and water buffalo were initially sorted as F. gigantica or F. hepatica, and crude proteins were extracted and subjected to SDS-PAGE. Cattle had the preponderance of F. gigantica, while F. hepatica was the dominant species in water buffaloes. Co-existing cattle and bubaline fasciolids revealed similar protein profile suggestive of a close genetic relationship. The distinct heavy bands shared by co-existing bubaline F. hepatica and F. gigantica relative to those detected in cattle fasciolids suggests a host...
Palavras-chave: Cattle & carabao Fasciola spp; Protein profile; SDS-PAGE; Philippines.
Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2382
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Detection of Genetic Relationship in the Tree of Life of Some Quail Through Molecular Markers Analyses BABT
Deef,Lamiaa Elsayed Mokhtar.
ABSTRACT Quail is an important and interesting group of galliform birds. The Common quail (Coturnix coturnix); the Japanese quail (Coturnix japonica); the Panda quail (Coturnix japonica); the Dotted white quail (Coturnix japonica) and the Bobwhite quail (Colinus virginianus) were used in this study. PCR-RFLP and SDS-proteins were performed to reveal the genetic characterization and genetic relationship of the studied quails. Analysis of fragments generated by digestion of PCR product with restriction enzyme NlaIII recorded highly polymorphic restriction profiles. There is a wide intraspecific COI, SEMA3E and TLX genes variability among the studied quails. Protein bands varied from10 to 18 between quails with minimum number of bands were in the Dotted...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Birds; PCR; Quail; SDS-PAGE; Galliformes.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100425
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Detection of the basement membrane-degrading proteolytic activity of Paracoccidioides brasiliensis after SDS-PAGE using agarose overlays containing Abz-MKALTLQ-EDDnp BJMBR
Puccia,R.; Juliano,M.A.; Juliano,L.; Travassos,L.R.; Carmona,A.K..
We have characterized, in the Paracoccidioides brasiliensis yeast phase, an exocellular SH-dependent serine proteinase activity against Abz-MKRLTL-EDDnp and analogous fluorescent-quenched peptides, and showed that it is also active against constituents of the basement membrane in vitro. In the present study, we separated the components of P. brasiliensis culture filtrates by electrophoresis and demonstrated that the serine-thiol exocellular proteinase has a diffuse and heterogeneous migration by SDS-PAGE, localizing in a region between 69 and 43 kDa. The hydrolytic activity was demonstrable after SDS-PAGE using buffered agarose overlays of Abz-MKALTLQ-EDDnp, following incubation at 37oC, and detection of fluorescent bands with a UV transilluminator....
Tipo: Info:eu-repo/semantics/article Palavras-chave: P. brasiliensis; Serine-thiol proteinase; SDS-PAGE; Fluorescent-quenched peptides.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500019
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Estimation of phylogenetic relationships of Phlomis species based on seed protein polymorphism Electron. J. Biotechnol.
Yüzbasioglu,Ertugrul; Dadandi,Mehmet Yasar; Özcan,Servet.
In this study, phylogenetic relationships among 39 Phlomis taxa were investigated based on seed protein profiles produced by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). A total of 21 polypeptide bands were scored, of which, 19 were polymorphic among the taxa of the genus Phlomis. A distance matrix was generated from the similarity matrix which was computed by using Jaccard’s similarity coefficients, based on polymorphic bands and then an UPGMA tree was established through cluster analysis performed on the distance matrix. Genetic distances ranged from 0.00 to 0.50 within subsection Dendrophlomis; from 0.00 to 0.625 within subsection Gymnophlomis and from 0.00 to 0.769 within subsection Oxyphlomis. The UPGMA tree formed...
Tipo: Journal article Palavras-chave: Phlomis; Phlomoides; Phlomis taxa; SDS-PAGE; Seed proteins.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200002
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Extração e análise eletroforética em gel de poliacrilamida (SDS-PAGE) de proteínas totais de folhas e raízes de Piper tuberculatum Acta Amazonica
Silva,Redinaldo dos Santos; Souza,Cláudia Regina Batista de.
O Estado do Pará é o principal produtor brasileiro de pimenta-do-reino (Piper nigrum Link), entretanto a sua produção tem sido bastante afetada pela doença conhecida como fusariose. O Fusarium solani f. sp. piperis é o agente causador desta doença que afeta o sistema radicular da planta, causando o apodrecimento das raízes e a queda das folhas levando à morte da planta. Algumas piperáceas nativas da região amazônica, entre elas a espécie Piper tuberculatum Jacq., têm se mostrado resistentes à infecção pelo F. solani f. sp. piperis, e desta forma têm sido utilizadas em estudos de interação planta-patógeno. Neste trabalho foram avaliadas cinco condições de extração de proteínas com o objetivo de selecionar tampões adequados para a extração de proteínas...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fusariose; Fusarium solani f. sp. piperis; Piper nigrum; Piper tuberculatum; Proteômica; SDS-PAGE.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672009000200002
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Growth characteristics and dynamics of protein synthesis in callus cultures from Glycine wightii (Wight & Arn.) Verdc. Ciência e Agrotecnologia
Silva,André Luis Coelho da; Caruso,Cecília Sulzbacher; Moreira,Renato de Azevedo; Horta,Ana Cecília Góes.
Cotyledon explants were first cultured on MS medium supplemented with 4.52 M 2,4-D and 0.46 mM kinetin. The development of the calli was followed (0, 4, 8, 12, 16, 20, 24, 28 and 32 days after) and the growth curve was determined, based in fresh and dry weight. The growth curve presented sigmoidal form with four distinct phases. The highest growth percentage was observed at the exponential phase and the lowest at the stationary phase. These results indicated that cotyledon callus subculture should be performed 20 days after inoculation. The calli obtained after a period of 28 days were freeze dried, macerated and submitted to extraction with buffers of different pH values (2.6; 4.0; 6.0; 8.0 and 10.0) and the proteins in the extracts were determined by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine; Leguminosae; Callogenesis; SDS-PAGE.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542005000600009
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Growth, Yield and Grain Nutritional Quality in Three Brazilian Pearl Millets (Pennisetum americanum L.) with African or Indian origins Anais da ABC (AABC)
MEDICI,LEONARDO O.; GONÇALVES,FABÍOLA V.; FONSECA,MARCOS PAULO S. DA; GAZIOLA,SALETE A.; SCHMIDT,DAIANA; AZEVEDO,RICARDO A.; PIMENTEL,CARLOS.
ABSTRACT In this study, we are presenting recommendations to the best agricultural use as well as for plant breeding of three millet cultivars namely ENA1 and ENA2, which have African origin, and BRS1501 originally from India. These cultivars were evaluated for growth, yield and grain quality traits. The morphological traits evaluated in this study indicated that the African genotypes ENA1 and ENA2 are better than the Indian genotype BRS1501 for no-till farming or to produce forage with 15% of crude protein at flowering and at harvest to produce stover (around 7% of crude protein content) for livestock feeding. The BRS1501 cultivar exhibited the highest values for total crude protein, albumins and prolamins, phytate and mineral contents in grains. ENA1 and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mineral density; SDS-PAGE; Phytate; Seed storage proteins; Amino acids.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000401749
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Haemolymph electrophoretic pattern of Ascia monuste orseis larvae (Lepidoptera: pieridae) parasitized by Cotesia glomerata (Hymenoptera: braconidae) J. Venom. Anim. Toxins incl. Trop. Dis.
Scaglia,M.; Brochetto-Braga,M. R.; Chaud-Netto,J.; Gobbi,N..
Cotesia glomerata is a natural enemy of the vegetable plague Ascia monuste orseis and preferably parasites 2nd, 3rd and 4th instar larvae. Parasitism effects on the haemolymph protein profile of Ascia monuste orseis larvae from the 2nd to 7th days were analyzed both qualitatively and quantitatively by SDS-PAGE and Coomassie-Blue binding methods. Quantitative analysis showed a progressive increase in the protein content of about 6.5 and 12.5 times in parasitized and non-parasitized larvae from the 2nd to 5th days, respectively. On the 6th day, a decrease in protein content was observed in both groups, although this decrease was significantly less than the control group that continued to metamorphosis. Meanwhile, parasitized larvae had one more day (7th day)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ascia monuste orseis; Cotesia glomerata; Electrophoretic protein profile; Haemolymph; Natural enemy; Parasitism; SDS-PAGE.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100006
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Improved adsorption-desorption extraction applied to the partial characterization of the antilisterial bacteriocin produced by Carnobacterium maltaromaticum C2 BJM
Tulini,F. L; De Martinis,E.C.P.
Bacteriocins are ribosomally produced peptides useful for food biopreservation. An improved adsorption-desorption process is proposed for the partial purification of the bacteriocin produced by the fish isolate Carnobacterium maltaromaticum C2. Analyzis of extract by SDS-PAGE indicated this method may offer an alternative to improve the yield of purification of bacteriocins.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacteriocins; LAB; Carnobacterium; SDS-PAGE.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200032
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Intraspecific variation in protein pattern of red scorpion (Mesobuthus tamulus, coconsis, pocock) venoms from Western and Southern India J. Venom. Anim. Toxins incl. Trop. Dis.
Badhe,R. V.; Thomas,A. B.; Harer,S. L.; Deshpande,A. D.; Salvi,N.; Waghmare,A..
Red scorpions Mesobuthus tamulus (Coconsis, Pocock) were obtained from different regions of West and South India (Ratnagiri, Chiplun and Ahmednagar from Maharashtra and Chennai from Tamil Nadu, respectively). Their venoms composition was analyzed using gel electrophoresis (SDS-PAGE). All venom samples shared six bands of 170, 80, 60, 57, 43, and 38 kDa molecular weights. Bands of 115 kDa and 51.5 kDa were characteristic of venoms obtained from red scorpions of Chiplun region, and the 26kDa band was absent in scorpion venom from Tamil Nadu. The separated protein band patterns suggest that the venoms from Ratnagiri, Ahmednagar and Tamil Nadu had high similarities in their biochemical composition but differed from that of Chiplun region. These data were also...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Red scorpion; SDS-PAGE; Mesobuthus tamulus.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400008
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Molecular analysis of endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.) BJM
Figueiredo,José Edson Fontes; Gomes,Eliane Aparecida; Guimarães,Claudia Teixeira; Lana,Ubiraci Gomes de Paula; Teixeira,Marta Aparecida; Lima,Guilherme Vitor Corrêa; Bressan,Wellington.
Endophytic bacteria play an important role in agriculture by improving plant performance and adaptation against biotic and abiotic stresses. In the present study molecular methods were used for identifying Bacillus endophytic bacteria isolated from Brazilian sweet corn. SDS-PAGE of wholecell protein extract of fortytwo isolates revealed a high number of scrutinable bands. Twenty-four isolates were identified in nine different groups of duplicated bacteria and eighteen were identified as unique. Some high-accumulated polipeptides with variable length were observed in almost isolates. Partial sequencing of 16S ribosomal gene revealed that all isolates are Bacillus sp. and among thirteen isolates with similar protein profiles, two were different strains....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic bacteria; Bacillus; Sweet corn; SDS-PAGE; RDNA sequencing.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300014
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Morfometria corpórea, características do sêmen, proteínas seminais e testosterona em cervos Cervus unicolor, em cativeiro Arq. Bras. Med. Vet. Zootec.
Chacur,M.G.M.; Martins,E.A.F.; Silva,A.A.; Guaberto,L.M.; Gabriel Filho,L.R.A.; Sanches,O.C.; Oba,E..
Objetivou-se estudar a morfometria corpórea, as características do sêmen, o perfil proteico do plasma seminal em SDS-PAGE e a concentração sérica de testosterona em cervos-sambar (Cervus unicolor), criados em cativeiro, na estação reprodutiva da primavera. Quatro machos com idades entre 12 e 36 meses foram avaliados em quatro momentos, com intervalos de sete dias, com peso corpóreo (60,5 a 89,0kg), índice de massa corporal (93,07kg/m2 a 126,56kg/m2), volume do ejaculado (0,50±0,35mL a 0,75±0,28mL), motilidade espermática (87,75±4,78% a 90,00±7,07%), defeitos totais (17,25±5,81% a 47,72±17,55%), testosterona sérica (6,43±4,33ng/dL a 166,00±64,48ng/dL) e proteínas do plasma seminal com bandas entre 7,6 e 142kDa. As características dos ejaculados não...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cervidae; Cativeiro; Sêmen; SDS-PAGE; Andrógeno.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000501392
Registros recuperados: 36
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