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Adaptação de método de extração e caracterização de proteínas em bactéria de importância para a indústria alimentícia. Infoteca-e
STEPHAN, M. P.; TEIXEIRA, K. R. dos S..
Tipo: Comunicado Técnico (INFOTECA-E) Palavras-chave: Proteína; Eletroforese; SDS-PAGE; Método de extração.
Ano: 2007 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/413478
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Análise da fosfoenolpiruvato carboxilase em genótipos de milho contrastantes em responsividade ao nitrogênio. Repositório Alice
PURCINO, A. A. C.; ROCHA, T.L.; FIGUEIREDO, J. E. F.; LOGUERCIO, L.L.; PORTILLO, N.C.; MARRIEL, I. E.; PARENTONI, S. N..
2000
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Milho; Nitrogenio; Enzima; Zea mays; Maize; Nitrogen; Gel; PEPC; SDS-PAGE; Enzyme.
Ano: 2000 URL: http://www.alice.cnptia.embrapa.br/handle/doc/484021
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Análise da glutamina sintetase em genótipos de milho contrastantes em eficiência de uso de nitrogênio. Repositório Alice
ROCHA, T.L.; FIGUEIREDO, J. E. F.; LOGUERCIO, L.L.; MARRIEL, I. E.; PARENTONI, S. N.; PURCINO, A. A. C..
2000
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Glutamina; Sintetase; Gel nativo; Nitrogenio; Eficiencia; Uso; Western Blot; SDS-PAGE; Glutamine; Synthetase; Nitrogen; Efficiency; Use.
Ano: 2000 URL: http://www.alice.cnptia.embrapa.br/handle/doc/483853
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Análise da glutamina sintetase em genótipos de milho contrastantes na eficiência do uso de nitrogênio. Repositório Alice
ROCHA, T.L.; FIGUEIREDO, J. E. F.; LOGUERCIO, L.L.; MARRIEL, I. E.; PARENTONI, S. N.; PURCINO, A. A. C..
2000
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Glutamina; Sintetase; Gel nativo; Nitrogenio; Eficiencia; Uso; Western Blot; SDS-PAGE; Glutamine; Synthetase; Nitrogen; Efficiency; Use.
Ano: 2000 URL: http://www.alice.cnptia.embrapa.br/handle/doc/484024
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Anopheles gambiae PRS1 modulates plasmodium development at both midgut and salivary gland steps Inra
Chertemps, T.; Mitri, C.; Perrot, S.; Sautereau, J.; Jacques, J.-C.; Thiery, I.; Bourgouin, C.; Rosinski-Chupin, I..
Background: Invasion of the mosquito salivary glands by Plasmodium is a critical step for malaria transmission. From a SAGE analysis, we previously identified several genes whose expression in salivary glands was regulated coincident with sporozoite invasion of salivary glands. To get insights into the consequences of these salivary gland responses, here we have studied one of the genes, PRS1 (Plasmodium responsive salivary 1), whose expression was upregulated in infected glands, using immunolocalization and functional inactivation approaches. Methodology/Principal Findings: PRS1 belongs to a novel insect superfamily of genes encoding proteins with DM9 repeat motifs of uncharacterized function. We show that PRS1 is induced in response to Plasmodium, not...
Tipo: Journal Article Palavras-chave: PLASMODIUM; MOUSTIQUE; GLANDE SALIVAIRE; SALIVE; MALADIE INFECTIEUSE; PROTEINE; TRANSMISSION DE LA MALADIE; VECTEUR DE LA MALADIE; INTERACTION MOLECULAIRE; RT-PCR; SDS-PAGE; ARTHROPODA; HEXAPODA; EXPRESSION DES GENES; MICROSCOPIE CONFOCALE; INSECTE; PARASITOSE MALARIA PARASITE; MOLECULAR-INTERACTIONS; BERGHEI SPOROZOITES; MOSQUITO; INVASION; INFECTION; PROTEIN; VECTOR; CELLS; FALCIPARUM.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010f5c08798&uri=/notices/prodinra1/2010/11/
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Assessment of genetic diversity among Pakistani wheat (Triticum aestivum L.) advanced breeding lines using RAPD and SDS-PAGE Electron. J. Biotechnol.
Ahmed,Muhammad Farooq; Iqbal,Muhammad; Masood,Muhammad Shahid; Rabbani,Malik Ashiq; Munir,Muhammad.
Genetic diversity was assessed among 32 advanced wheat breeding lines included in the National Uniform Wheat Yield Trials (2006-07) of Pakistan using molecular (DNA) and biochemical (SDS-PAGE) markers. Of the 72 RAPD primers used for initial screening, 15 were found polymorphic. A total of 140 bands (61.4% polymorphic) were generated by the 15 random decamer primers. Genetic similarity coefficients ranged from 0.81 to 0.94 for rainfed and from 0.70 to 0.93 for the normal seeding date group. Cluster analysis using the unweighted pair group method of arithmetic averages (UPGMA) clustered the 32 advanced wheat breeding lines into one major and three small groups. Maximum level of polymorphism (90%) was observed for the primer OPA-05. Lines N9 and N11 showed...
Tipo: Journal article Palavras-chave: Genetic diversity; Molecular markers; Polymorphism; RAPD; SDS-PAGE; Wheat.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300001
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Buffalo mozzarella chemical composition and authenticity assessment by electrophoretic profiling. Repositório Alice
GONÇALVES, B. R. F.; SILVA, G. de J.; CONCEIÇÃO, D. G.; EFITO, A. S. do; FERRÃO, S. P. B..
Abstract: Buffalo milk mozzarella is often adulterated by the addition of cow's milk. The aim of this study was to assess the quality of buffalo milk mozzarella by using electrophoresis (SDS-PAGE) to detect the addition of cow's milk. Reference cheeses were produced exclusively from combinations of buffalo and cow milk, and labeled reference treatment buffalo or cow (RTB and RTC, respectively). Standardized cheeses were made by combining buffalo milk and 2.5%, 5.0%, 10%, 20%, 30%, 40% or 50% cow's milk. A total of 9 cheese formulations were produced and either frozen immediately (time 0) or after 20 days (time 20). Eighteen commercial samples of buffalo mozzarella were sampled between 0-20 days of production. The chemical composition (moisture, ash, fat in...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: SDS-PAGE; Fraude; Leite de búfala; Mussarela; Mozzarella; Búfalo; Queijo; Adulteração; Peptídeo; Proteína; Produto derivado do leite; Análise microbiológica; Controle de qualidade; Buffalo milk; Cheese; Food adulteration; Peptides; Microbiology; Microbiological analysis; Feed quality; Quality controls.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1087574
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Caracterização de bactérias endofíticas isoladas do milho cultivado no agroecossistema cerrado. Repositório Alice
COELHO, V. T. S.; QUINTÃO, P. L.; PAOLI, H. C.; MELO, M. A. V.; LAGE, G. A.; FONSECA, P. C.; BRESSAN, W.; GUIMARAES, C. T.; GOMES, E. A.; FIGUEIREDO, J. E. F..
2002
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: SDS-PAGE; RAPD-PCR; RDNA; Sequenciamento; Taxinomia.
Ano: 2002 URL: http://www.alice.cnptia.embrapa.br/handle/doc/485688
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Caracterización de las inmunoglobulinas del ñandú (Rhea americana) InVet
Montagna,D.R.; Rigo,V; Ramayo,L.G.; Goldman,L.H.; Huguet,M.J.; Maceira,N; Jar,A.M..
El objetivo de este trabajo fue caracterizar las inmunoglobulinas séricas y la IgY de la yema de huevo de ñandú, a través de las técnicas de electroforesis en acetato de celulosa, electroforesis en gel de poliacrilamida y Western-blot. Se estudiaron 19 ñandúes clínicamente sanos de 14 a 15 meses de edad, provenientes un criadero de Balcarce, Argentina. La electroforesis en acetato de celulosa de los sueros mostró la presencia de cuatro fracciones proteicas: albúmina, alfa, beta y gamma globulinas. La concentración de las gamma globulinas fue de 0,69 ± 0,18 g/dl, con un rango de 0,42-1,05 g/dl. La corrida electroforética de la IgY muestró que esta se ubica en la fracción gammaglobulínica. La electroforesis en gel de poliacrilamida evidenció, tanto en suero...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ã‘andú; Electroforesis; SDS-PAGE.
Ano: 2013 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982013000200002
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Characterization of cry2-type genes of Bacillus thuringiensis strains from soil-isolated of Sichuan basin, China BJM
Liang,Hongxia; Liu,Yao; Zhu,Jun; Guan,Peng; Li,Shuangcheng; Wang,Shiquan; Zheng,Aiping; Liu,Huainian; Li,Ping.
Sichuan basin, situated in the west of China, is the fourth biggest basin in China. In order to describe a systematic study of the cry2-type genes resources from Bacillus thuringiensis strains of Sichuan basin, a total of 791 Bacillus thuringiensis strains have been screened from 2650 soil samples in different ecological regions. The method of PCR-restriction fragment length polymorphism (PCR-RFLP) was used to identify the type of cry2 genes. The results showed that 322 Bacillus thuringiensis strains harbored cry2-type genes and four different RFLP patterns were found. The combination of cry2Aa/cry2Ab genes was the most frequent (90.4%), followed by cry2Aa (6.8%) and cry2Ab alone (2.5%), and only one novel type of cry2 gene was cloned from one isolate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus thuringiensis; PCR-RFLP; SDS-PAGE; Novel cry2-type gene.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100018
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Characterization of onion (Allium cepa L.) varieties based on physical properties and seed performance Agrociencia
Herrera-Corredor,Carlos; Carrillo-Castañeda,Guillermo.
Abstract: Five cultivars (Cristal White Wax, Toro, Red Burgundy, Cojumatlán and Contessa) of onion (Allium cepa L.) were characterized according to: the weight of 100 seeds, germination kinetics, speed germination (T50) and seed protein analysis. Seed of Crystal White Wax was differentiated from the other cultivars by its low weight (0.30 g), since the weight of the rest was 0.40 g. Crystal White Wax had low seed germination (56%) and the highest delay of germination (T50 values were 78 h), while T50 of the rest was 52 h. Protein was isolated from seeds at the precise moment of seed germination. All cultivars were differentiated by their pattern and number of proteins resolved by SDS-PAGE electrophoresis. Toro and Red Burgundy showed the greater difference...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allium cepa L.; Germination kinetics; SDS-PAGE; T50; Vigor.
Ano: 2007 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952007000700755
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Comparative electrophoretic profile of proteins and esterases in healthy silkworm larvae (Bombyx mori Lineu, 1758) and infected with nucleopolyhedrovirus - doi: 10.4025/actascibiolsci.v35i1.8612 Biological Sciences
Ruiz, Juliana Bueno; Universidade Estadual de Maringá; Ronqui, Ludimilla; Universidade Federal de Rondonia; Oliveira, Jussara Ricardo de; Universidade Paranaense; Ruvolo-Takasusuki, Maria Claudia Colla; Universidade Estadual de Maringá.
Total proteins and esterases from silk gland extracts of Bombyx mori silkworm were characterized and compared with electrophoretic profiles of prepared extracts with silkworm glands infected with nucleopolyhedrovirus (BmNPV). SDS-PAGE (7%) gels was used for the total proteins, and 10% native PAGE for esterases. In the silk glands extracts of healthy silkworms, it was observed seven protein zones with molecular weight varying between 10 kDa (P1) and 60 kDa (P7). In the infected silkworms, a new zone named P8 (90 kDa) was also detected. Esterases activity at 5th instar larvae underwent changes after the infection with BmNPV, since there was a reduction (EST-6 and EST-7) and an increase (EST-8) in the intensity of the regions of esterases activity, and...
Tipo: Genética Bioquímica Palavras-chave: 2.02.04.00-0 viral infection; Silk gland; Isoenzymes; SDS-PAGE; Molecular markers 2.02.02.00-8.
Ano: 2012 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/8612
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Comparison of electrophoretic protein profiles of Campylobacter jejuni subsp. jejuni isolated from different animal species BJM
Scarcelli,Eliana; Costa,Elizabeth Oliveira da; Genovez,Margareth Élide; Cardoso,Maristela Vasconcellos; Bach,Erna Elizabeth; Torres,Ana Paula.
Electrophoretic protein profiles of Campylobacter jejuni subsp. jejuni strains isolated from feces of seven animal species, including man, were compared. Fourteen strains (two from each species) plus two human strains and the reference one, were ruptured by ultrasound and their total soluble proteins were analyzed by SDS-PAGE technique in a 12% polyacrylamide gel with computerized densitometric reading by the molecular analyst software. All the strains had bands in common that correspond to 45 and 66 Kda molecular weight. The disagreement corresponded to a 97 to 200 Kda molecular weight region. From the 17 strains, 13 (76.5%), were classified as biotype I, three (17.6%) as biotype II and one (5.8%) as biotype III. Since protein extracts were obtained from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Campylobacter jejuni; SDS-PAGE; Biotyping.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400006
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Comparison of protein profile of co-existing Fasciola hepatica and Fasciola gigantica parasite in Bos taurus (cattle) and Bubalus bubalis (Philippine water buffalo) OAK
De Vera, M.E; Sato, K; Oyong, G; Claveria, F.G.
Fasciola hepatica and Fasciola gigantica habitually co-exist as parasites of cattle (Bos taurus) and water buffalo (Bubalus bubalis), and despite variation in their morphometry, their species status is suspicious. Liver flukes isolated from cattle and water buffalo were initially sorted as F. gigantica or F. hepatica, and crude proteins were extracted and subjected to SDS-PAGE. Cattle had the preponderance of F. gigantica, while F. hepatica was the dominant species in water buffaloes. Co-existing cattle and bubaline fasciolids revealed similar protein profile suggestive of a close genetic relationship. The distinct heavy bands shared by co-existing bubaline F. hepatica and F. gigantica relative to those detected in cattle fasciolids suggests a host...
Palavras-chave: Cattle & carabao Fasciola spp; Protein profile; SDS-PAGE; Philippines.
Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2382
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Detection of Genetic Relationship in the Tree of Life of Some Quail Through Molecular Markers Analyses BABT
Deef,Lamiaa Elsayed Mokhtar.
ABSTRACT Quail is an important and interesting group of galliform birds. The Common quail (Coturnix coturnix); the Japanese quail (Coturnix japonica); the Panda quail (Coturnix japonica); the Dotted white quail (Coturnix japonica) and the Bobwhite quail (Colinus virginianus) were used in this study. PCR-RFLP and SDS-proteins were performed to reveal the genetic characterization and genetic relationship of the studied quails. Analysis of fragments generated by digestion of PCR product with restriction enzyme NlaIII recorded highly polymorphic restriction profiles. There is a wide intraspecific COI, SEMA3E and TLX genes variability among the studied quails. Protein bands varied from10 to 18 between quails with minimum number of bands were in the Dotted...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Birds; PCR; Quail; SDS-PAGE; Galliformes.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100425
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Detection of the basement membrane-degrading proteolytic activity of Paracoccidioides brasiliensis after SDS-PAGE using agarose overlays containing Abz-MKALTLQ-EDDnp BJMBR
Puccia,R.; Juliano,M.A.; Juliano,L.; Travassos,L.R.; Carmona,A.K..
We have characterized, in the Paracoccidioides brasiliensis yeast phase, an exocellular SH-dependent serine proteinase activity against Abz-MKRLTL-EDDnp and analogous fluorescent-quenched peptides, and showed that it is also active against constituents of the basement membrane in vitro. In the present study, we separated the components of P. brasiliensis culture filtrates by electrophoresis and demonstrated that the serine-thiol exocellular proteinase has a diffuse and heterogeneous migration by SDS-PAGE, localizing in a region between 69 and 43 kDa. The hydrolytic activity was demonstrable after SDS-PAGE using buffered agarose overlays of Abz-MKALTLQ-EDDnp, following incubation at 37oC, and detection of fluorescent bands with a UV transilluminator....
Tipo: Info:eu-repo/semantics/article Palavras-chave: P. brasiliensis; Serine-thiol proteinase; SDS-PAGE; Fluorescent-quenched peptides.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500019
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Discriminação de linhagens de milhos normais e QPMs por meio de fração zeínas. Repositório Alice
BARBOSA, F. C. L.; SOUZA, I. R. P.; PACHECO, C. A. P..
2006
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Zea mays; SDS-PAGE; Focalização isoelétrica; Linhagens; Ponto isoelétrico (pl).
Ano: 2006 URL: http://www.alice.cnptia.embrapa.br/handle/doc/490234
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Diversidade genética de Colletotrichum graminicola isolados da cultura do sorgo no ecossistema agrícola tropical revelada por SDS-PAGE, RAPD e análise da sequência do RDNA 18s e região intergênica 18s-28s Repositório Alice
PAOLI, H.C.; QUINTAO, P.L.; COELHO, V.T.S.; LAGE, G.A.; FONSECA, P.C.; FERREIRA, A.S.; CASELA, C.R.; GUIMARAES, C. T.; GOMES, E.A.; FIGUEIREDO, J. E. F..
2002
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: RDNA; RAPD-PCR; ARDRA; Sequenciamento; SDS-PAGE.
Ano: 2002 URL: http://www.alice.cnptia.embrapa.br/handle/doc/485693
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Eletroforese bidimensional na análise de zeinas em milhos indigenas. Repositório Alice
LANA, U. G. P.; SOUZA, I. R. P.; CARNEIRO, N. P.; GUIMARAES, C. T.; PAIVA, E..
2002
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Zea mays L; Endosperma; Focalizacao isoeletrica; SDS-PAGE.
Ano: 2002 URL: http://www.alice.cnptia.embrapa.br/handle/doc/485694
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Estimation of phylogenetic relationships of Phlomis species based on seed protein polymorphism Electron. J. Biotechnol.
Yüzbasioglu,Ertugrul; Dadandi,Mehmet Yasar; Özcan,Servet.
In this study, phylogenetic relationships among 39 Phlomis taxa were investigated based on seed protein profiles produced by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). A total of 21 polypeptide bands were scored, of which, 19 were polymorphic among the taxa of the genus Phlomis. A distance matrix was generated from the similarity matrix which was computed by using Jaccard’s similarity coefficients, based on polymorphic bands and then an UPGMA tree was established through cluster analysis performed on the distance matrix. Genetic distances ranged from 0.00 to 0.50 within subsection Dendrophlomis; from 0.00 to 0.625 within subsection Gymnophlomis and from 0.00 to 0.769 within subsection Oxyphlomis. The UPGMA tree formed...
Tipo: Journal article Palavras-chave: Phlomis; Phlomoides; Phlomis taxa; SDS-PAGE; Seed proteins.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200002
Registros recuperados: 49
Primeira ... 123 ... Última
 

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