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A naturally occurring mutation K220T in the pleiotropic activator PrfA of Listeria monocytogenes results in a loss of virulence due to decreasing DNA-binding affinity Inra
Velge, P.; Herler, M.; Johansson, J.; Roche, S.; Témoin, S.; Fedorov, A. A.; Gracieux, P.; Almo, S. C.; Goebel, W.; Cossart, P..
The sequencing of prfA, encoding the transcriptional regulator of virulence genes, in 26 low virulence field Listeria monocytogenes strains showed that eight strains exhibited the same single amino-acid substitution: PrfAK220T. These strains exhibited no expression of PrfA-regulated proteins and thus no virulence. This substitution inactivated PrfA, since expression of the PrfAK220T mutant gene in an EGDΔprfA strain did not restore the haemolytic and phosphatidylcholine phospholipase C activities, in contrast to the wild-type prfA gene. The substitution of the lysine at position 220 occurred in the helix αH. However, the data showed that the PrfAK220T protein is dimerized just as well as its wild-type counterpart, but does not bind to PrfA boxes....
Tipo: Journal Article Palavras-chave: LISTERIA MONOCYTOGENES; PROTEINE; FACTEUR DE VIRULENCE; BACTERIE PATHOGENE; ADN; SEQUENCE NUCLEOTIDIQUE; REGULATION; TRANSCRIPTION; VIRULENCE; EXPRESSION DES GENES; ACIDE AMINE; MUTATION; RT-PCR; ELECTROPHORESE; ENZYME; ARN POLYMERASE.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009c1895b3c&uri=/notices/prodinra1/2009/11/
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A physical map of the heterozygous grapevine 'Cabernet Sauvignon' allows mapping candidate genes for disease resistance Inra
Moroldo, M.; Paillard, S.; Marconi, R.; Legeai, F.; Canaguier, A.; Cruaud, C.; de Berardinis, V.; Guichard, C.; Brunaud, V.; Le Clainche, I.; Scalabrin, S.; Testolin, R.; di Gaspero, G.; Morgante, M.; Adam-Blondon, A.F..
Background: Whole-genome physical maps facilitate genome sequencing, sequence assembly, mapping of candidate genes, and the design of targeted genetic markers. An automated protocol was used to construct a Vitis vinifera 'Cabernet Sauvignon' physical map. The quality of the result was addressed with regard to the effect of high heterozygosity on the accuracy of contig assembly. Its usefulness for the genome-wide mapping of genes for disease resistance, which is an important trait for grapevine, was then assessed. Results: The physical map included 29,727 BAC clones assembled into 1,770 contigs, spanning 715,684 kbp, and corresponding to 1.5-fold the genome size. Map inflation was due to high heterozygosity, which caused either the separation of allelic...
Tipo: Journal Article Palavras-chave: MILDIOU; MALADIE FONGIQUE; CARTE GENETIQUE; SYSTEME IMMUNITAIRE; VIGNE; RESISTANCE AUX MALADIES; CARTOGRAPHIE; GENE CANDIDAT; GENETIQUE; SEQUENCE NUCLEOTIDIQUE; CABERNET SAUVIGNON PLANT IMMUNE SYSTEM; VITIS VINIFERA L.; NONHOST RESISTANCE; POWDERY MILDEW; GENOME SEQUENCE; LINKAGE MAP; RICE GENOME; BAC; ARABIDOPSIS; GENETICS.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011dc1cb8fa&uri=/notices/prodinra1/2011/03/
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A worldwide survey of tomato yellow leaf curl viruses Inra
Czosnek, H.; Laterrot, H..
The name tomato yellow leaf curl virus (TYLCV) has been given to several whitefly-transmitted geminiviruses affecting tomato cultures in many tropical and subtropical regions. Hybridization tests with two DNA probes derived from a cloned isolate of TYLCV from Israel (TYLCV-ISR) were used to assess the affinities of viruses in naturally infected tomato plants with yellow leaf curl or leaf curl symptoms from 25 countries. Probe A which included most of the intergenic region was expected to detect only isolates closely related to TYLCV-ISR, especially after high stringency washes. In contrast probe B, which included the full-length genome, was expected to detect a wide range of whitefly-transmitted geminiviruses. Tomato samples from six countries in the...
Tipo: Journal Article Palavras-chave: VIRUS DE L'ENROULEMENT JAUNE DE LA TOMATE; GEMINIVIRUS; BEMISIA TABACI; VIRUS DES VEGETAUX; SEQUENCE NUCLEOTIDIQUE; VECTEUR DE MALADIE; DISTRIBUTION GEOGRAPHIQUE.
Ano: 1997 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9700027877065437&uri=/notices/prodinra1/2010/11/
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Amylosucrase, a glucan-synthesizing enzyme from the α-amylase family Inra
Skov, L.K.; Mirza, O.; Henriksen, A.; Potocki de Montalk, G.; Remaud-Simeon, M.; Sarçabal, P.; Willemot, R.M.; Monsan, P.; Gajhede, M..
Amylosucrase (E.C. 2.4.1.4) is a member of Family 13 of the glycoside hydrolases (the α-amylases), although its biological function is the synthesis of amylose-like polymers from sucrose. The structure of amylosucrase from Neisseria polysaccharea is divided into five domains: an all helical N-terminal domain that is not similar to any known fold, a (β/α)8-barrel A-domain, B- and B′-domains displaying α/β-structure, and a C-terminal eight-stranded β-sheet domain. In contrast to other Family 13 hydrolases that have the active site in the bottom of a large cleft, the active site of amylosucrase is at the bottom of a pocket at the molecular surface. A substrate binding site resembling the amylase 2 subsite is not found in amylosucrase. The site is blocked by a...
Tipo: Journal Article Palavras-chave: AMYLOSUCRASE; BACTERIE NON PATHOGENE; NEISSERIA POLYSACCHAREA; ALPHA AMYLASE; SACCHAROSE; STRUCTURE TRIDIMENSIONNELLE; GLYCOGENE; SEQUENCE NUCLEOTIDIQUE; ACTIVITE ENZYMATIQUE; GLYCOSIDE HYDROLYSE; NEISSERICEAE; BACTERIE GRAM NEGATIF; SUCRE.
Ano: 2001 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0400023316102157&uri=/notices/prodinra1/2010/10/
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An important step in Listeria lipoprotein research Inra
Garcia-del Portillo, F.; Cossart, P..
Over the last 10 years, DNA sequences of more than 600 bacterial species have been deposited in databases and are now available to search any gene, motif, or regulatory sequence of interest. Although genome data are instrumental in phylogenetic analysis and in silico design of metabolic and regulatory networks, only a very small fraction of the information has been experimentally validated. A striking example is lipoproteins predicted from genome sequences. Despite the predominance of this class of surface proteins in bacteria (up to 0.5 to 8% of the proteome), very few of these proteins have been identified as lipoproteins by biochemical methods (19). In this issue of the Journal of Bacteriology, Baumgärtner et al. (2) report a systematic...
Tipo: Journal Article Palavras-chave: LIPOPROTEINE; LISTERIA MONOCYTOGENES; VIRULENCE; BACTERIE GRAM-POSITIVE; BACTERIE GRAM-NEGATIVE; BACTERIE PATHOGENE; ADN; SEQUENCE NUCLEOTIDIQUE; ANALYSE PHYLOGENETIQUE; REGULATION; PROTEINE; SURFACE CELLULAIRE; VIRULENCE; TECHNIQUE ANALYTIQUE; BIOCHIMIE.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009594c400a&uri=/notices/prodinra1/2009/11/
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Anaerobic sugar catabolism in Lactococcus lactis: genetic regulation and enzyme control over pathway flux Inra
Cocaign-Bousquet, M.; Even, S.; Lindley, N.D.; Loubière, P..
Lactic acid bacteria and particularly Lactococcus lactis are widely used for the production of lactic acid in fermented foods. Control of the catabolic rate in L. lactis, i.e., the rate of lactic acid production, appears to be determinant for dairy product quality. While the mechanisms involved in control have not been totally elucidated, they seem to depend upon the strain and the growth conditions. Furthermore, it remains unclear whether the catabolic rate is controlled at the level of transcription, translation or enzyme activity. The recent sequencing of the L. lactis genome has brought novel insights to physiologic studies of the bacteria. This review discusses both genetic information and metabolic studies concerning anaerobic sugar...
Tipo: Journal Article Palavras-chave: ANAEROBIE; REGULATION GENETIQUE; CATABOLISME; CROISSANCE BACTERIENNE; FACTEUR DU MILIEU; SEQUENCE NUCLEOTIDIQUE; PYRUVATE; EXPRESSION DES GENES; TRANSCRIPTION.
Ano: 2002 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0400023343102184&uri=/notices/prodinra1/2010/09/
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Analysis of CATMA transcriptome data identifies hundreds of novel functional genes and improves gene models in the Arabidopsis genome Inra
Aubourg, S.; Martin-Magniette, M.L.; Brunaud, V.; Taconnat, L.; Bitton, F.; Balzergue, S.; Jullien, P.E.; Ingouff, M.; Thareau, V.; Schiex, T.; Lecharny, A.; Renou, J.P..
Background: Since the finishing of the sequencing of the Arabidopsis thaliana genome, the Arabidopsis community and the annotator centers have been working on the improvement of gene annotation at the structural and functional levels. In this context, we have used the large CATMA resource on the Arabidopsis transcriptome to search for genes missed by different annotation processes. Probes on the CATMA microarrays are specific gene sequence tags (GSTs) based on the CDS models predicted by the Eugene software. Among the 24 576 CATMA v2 GSTs, 677 are in regions considered as intergenic by the TAIR annotation. We analyzed the cognate transcriptome data in the CATMA resource and carried out data-mining to characterize novel genes and improve gene models....
Tipo: Journal Article Palavras-chave: ADNC; EXPRESSION DES GENES; SEQUENCE NUCLEOTIDIQUE; ARABIDOPSIS THALIANA; GENOME; MODELE; METHODE STATISTIQUE; PROTEINE; TRANSCRIPTION; ARN MESSAGER; PCR; RT-PCR CDNA MICROARRAY DATA; EXPRESSION PROFILES; SEQUENCE TAGS; ANNOTATION; THALIANA; NORMALIZATION; REGIONS; TOOLS.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20116115e9a4&uri=/notices/prodinra1/2011/03/
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Analysis of the population structure of three phenotypically different PSTVd isolates Inra
Gora, A.; Candresse, T.; Zagorski, W..
Phenotypically dissimilar greenhouse isolates from a Polish collection of potato spindle tuber viroid (PSTVd) were analysed. Partially purified PSTVd genomic RNAs from severe, intermediate and mild isolates was reverse transcribed and the resulting cDNAs enzymatically amplified. Abutting-primer PCR (Ab-P PCR) technology was used to obtain, in a single step, infectious full-length PSTVd cDNA monomers and these were sequenced. The mild isolate was found to be composed of a unique molecular variant (M), closely related to previously described PSTVd mild isolates. In the intermediate isolate, three variants, i2, i3 and i4, were detected. The severe isolate was found to be a mixture containing at least four molecular variants: s23, s27, i4 and i2. Infection of...
Tipo: Journal Article Palavras-chave: PSTVD; VIRUS DE LA POMME DE TERRE; VIROIDE PHYTOPATHOGENE; ISOLAT; SYMPTOMATOLOGIE; SEQUENCE NUCLEOTIDIQUE.
Ano: 1994 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9500010807048220&uri=/notices/prodinra1/2010/11/
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Biological and sequence analysis of a novel European isolate of Barley mild mosaic virus that overcomes the barley rym5 resistance gene Inra
Kanyuka, K.; McGrann, G.; Alhudaib, K.; Hariri, D.; Adams, M.J..
A Barley mild mosaic virus (BaMMV) isolate from France (BaMMV-Sil) capable of overcoming rym5-controlled resistance was inoculated to barley genotypes carrying various genes for resistance to the barley mosaic viruses. BaMMV-Sil was unable to infect genotypes carrying rym1, rym4, rym8, rym9, or rym11 but genotypes carrying rym3, rym5, rym6 or no known bymovirus resistance gene were susceptible. Plants carrying rym7 or rym10 showed partial resistance with delayed virus accumulation. The two genomic RNAs of BaMMV-Sil were sequenced and compared to published sequences and those of a further common strain isolate from the UK. Four amino acid differences were observed between BaMMV-Sil and European common strain isolates in the polypeptide encoded by RNA1, the...
Tipo: Journal Article Palavras-chave: BARLEY MILD MOSAIC VIRUS; SEQUENCE NUCLEOTIDIQUE; PLANTE; HORDEUM; VIRUS MOSAIQUE DE L'ORGE; GEEN DE RESISTANCE.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0500027585106777&uri=/notices/prodinra1/2010/10/
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CATdb: a public access to Arabidopsis transcriptome data from the URGV-CATMA platform Inra
Gagnot, S.; Tamby, J.P.; Martin-Magniette, M.L.; Bitton, F.; Taconnat, L.; Balzergue, S.; Aubourg, S.; Renou, J.P.; Lecharny, A.; Brunaud, V..
CATdb is a free resource available at http://urgv.evry.inra.fr/CATdb that provides public access to a large collection of transcriptome data for Arabidopsis thaliana produced by a single Complete Arabidopsis Transcriptome Micro Array (CATMA) platform. CATMA probes consist of gene-specific sequence tags (GSTs) of 150500 bp. The v2 version of CATMA contains 24 576 GST probes representing most of the predicted A. thaliana genes, and 615 probes tiling the chloroplastic and mitochondrial genomes. Data in CATdb are entirely processed with the same standardized protocol, from microarray printing to data analyses. CATdb contains the results of 53 projects including 1724 hybridized samples distributed between 13 different organs, 49 different developmental...
Tipo: Journal Article Palavras-chave: CDNA MICROARRAY DATA; SEQUENCE TAGS; INFORMATION; GENOME; NORMALIZATION; BIOLOGY; GENE COMPONENTS ; ADNC; SEQUENCE NUCLEOTIDIQUE; NORMALISATION; BIOLOGIE; ARABIDOPSIS THALIANA; MUTATION; MITOCHONDRIE; CHLOROPLASTE; METHODE STATISTIQUE; EXPRESSION DES GENES.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20111a416d88&uri=/notices/prodinra1/2011/03/
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Characterisation of a new potyvirus species infecting meadow saffron (Colchicum autumnale) Inra
Poutaraud, A.; Desbiez, C.; Lemaire, O.; Lecoq, H.; Herrbach, E..
The alkaloids contained in Colchicum autumnale seeds are used in numerous medicines. Good quality seeds are difficult to obtain from this undomesticated plant. Therefore, a research program was set up aiming to cultivate C. autumnale in order to improve alkaloid contents and seed yields. In this context, a collection was established in 1999 by transplanting corms from twelve different locations in Eastern France. However, serious symptoms of necrosis and decay have appeared in this collection since 2001. Electron microscopic observations of plants showing symptoms revealed the presence of filamentous particles and pinwheel-like structures characteristic of the Potyviridae family. Leaves and corms from symptomatic plants were assayed with potyvirus-specific...
Tipo: Journal Article Palavras-chave: COLCHICUM AUTUMNALE; LILIACEAE; PLANTE TOXIQUE; POTYVIRUS; RT-PCR; MSBV; MEADOW SAFFRON BREAKING VIRUS; GENOME VIRAL; VIRUS DES VEGETAUX; ALCALOIDE; TEST ELISA; SEQUENCE NUCLEOTIDIQUE.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0400031229104456&uri=/notices/prodinra1/2010/10/
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Cloning of a cDNA encoded by a member of the Arabidopsis thaliana ATP sulfurylase multigene family. Expression studies in yeast and in relation to plant sulfur nutrition Inra
Logan, H.M.; Cathala, N.; Grignon, C.; Davidian, J.C..
Tipo: Journal Article Palavras-chave: ARABIDOPSIS THALIANA; ATP; SULFURYLASE; CLONAGE MOLECULAIRE; ADNC; SEQUENCE NUCLEOTIDIQUE; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGUE; ACTIVITE ENZYMATIQUE; TRANSCRIPTION; SULFATE; BRASSICA NAPUS.
Ano: 1996 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0200029830090142&uri=/notices/prodinra1/2010/09/
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Coat protein gene sequences of garlic and onion isolates of the onion yellow dwarf potyvirus (OYDV) Inra
Kobayashi, K.; Rabinowicz, P.; Bravo-Almonacid, F.; Helguerra, M.; Conci, V.; Lot, H.; Mentaberry, A..
Partial genomic sequences from an unknown garlic potyvirus and from an onion isolate of the onion yellow dwarf potyvirus (OYDV) were obtained. Comparison of the deduced amino acid sequences showed a similarity of 88% between the respective viral coat proteins. The garlic potyvirus coat protein was expressed inE. coli cells, purified, and subjected to Western blot analysis using antibodies raised against different garlic-infecting viruses. The expression protein was consistently recognised by anti-OYDV antibodies and did not react with antibodies specific for leek yellow stripe potyvirus (LYSV), garlic common latent carlavirus (GCLV) and shallot latent carlavirus (SLV). Besides, the garlic potyvirus coat protein was obtained as a fusion protein and used as...
Tipo: Journal Article Palavras-chave: VIRUS DE LA JAUNISSE NANISANTE DE L'OIGNON; PROTEINE CAPSIDE; VIRUS DES VEGETAUX; SEQUENCE NUCLEOTIDIQUE.
Ano: 1996 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9700027683062318&uri=/notices/prodinra1/2010/11/
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Comparative functional study of the viral telomerase RNA based on natural mutations Inra
Fragnet, L.; Kut, E.; Rasschaert, D..
Telomerase activity is present in most malignant tumors and provides a mechanism for the unlimited potential for division of neoplastic cells. We previously characterized the first identified viral telomerase RNA (vTR) encoded by the Marek's disease virus (MDV) (Fragnet, L., Blasco, M. A., Klapper, W., and Rasschaert, D. (2003) J. Virol. 77, 5985–5996). This avian herpesvirus induces T-lymphomas. We demonstrated that the vTR subunit of the oncogenic MDV-RB1B strain is functional and would be more efficient than its chicken counterpart, cTR, which is 88% homologous. We take advantage of the functionality of those natural mutant TRs to investigate the involvement of the mutations of vTR on its efficiency in a heterologous murine cell system and in a...
Tipo: Journal Article Palavras-chave: ACTIVITE; TELOMERASE; SEQUENCE NUCLEOTIDIQUE.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0500018081108634&uri=/notices/prodinra1/2010/11/
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Complete nucleotide sequence of the genome of a severe cherry isolate of apple chlorotic leaf spot trichovirus (ACLSV) Inra
German-Retana, S.; Bergey, B.; Delbos, R.P.; Candresse, T.; Dunez, J..
 The genome of the Balaton1 severe cherry isolate of apple chlorotic leaf spot trichovirus (ACLSV-Bal1) has been cloned and sequenced. The genomic RNA is 7 549 nucleotide long, excluding the poly A tail. The genomic organization, with three overlapping open reading frames (ORF), is similar to that of the other sequenced ACLSV isolates. Sequence comparisons indicate a high variability between ACLSV isolates, with overall nucleotide sequence homology levels between 76 and 82%. The coat protein, encoded internally inside a larger ORF, is the most conserved protein (identity levels between 87 and 93%) while the central ORF, encoding the putative movement protein, is the most divergent (77 to 85% identity).
Tipo: Journal Article Palavras-chave: TRICHOVIRUS; ARN GENOMIQUE; SEQUENCE NUCLEOTIDIQUE; VIRUS DES VEGETAUX.
Ano: 1997 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9700011373064160&uri=/notices/prodinra1/2010/11/
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Conformational dynamics underlie the activity of the auxin-binding protein, Nt-abp1 Inra
David, K.; Carnero-Diaz, E.; Leblanc, N.; Monestiez, M.; Grosclaude, J.; Perrot-Rechenmann, C..
The auxin-binding protein 1 (ABP1) has been proposed to be involved in the perception of the phytohormone at the plasma membrane. Site-directed mutagenesis was performed on highly conserved residues at the C terminus of ABP1 to investigate their relative importance in protein folding and activation of a functional response at the plasma membrane. Detailed analysis of the dynamic interaction of the wild-type ABP1 and mutated proteins with three distinct monoclonal antibodies recognizing conformation-dependent epitopes was performed by surface plasmon resonance. The influence of auxin on these interactions was also investigated. The Cys177 as well as Asp175 and Glu176 were identified as critical residues for ABP1 folding and action at the plasma membrane. On...
Tipo: Journal Article Palavras-chave: PHYTOHORMONE; MEMBRANE PLASMIQUE; SEQUENCE NUCLEOTIDIQUE; CONFORMATION.
Ano: 2001 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0300012809095526&uri=/notices/prodinra1/2010/10/
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Constitution d'une génothèque destinée à l'identification des génomes mitochondriaux et recherche de nouvelles séquences mitochondriales chez les végétaux supérieurs Inra
Lejeune, B.; Pereira de Souza, A.; Haouazine, N.; Jubier, M.F.; Besin, E.; Lancelin, D.; Pelletier, G..
Le génome mitochondrial (mt) des plantes supérieures, dont la taille peut varier entre 200 et 560 kb, est composé d’une population hétérogène de molécules de tailles variables pouvant se recombiner grâce à la présence de séquences répétées. Les gènes présents sont constitués de séquences codantes très conservées et peuvent présenter des caractéristiques structurales comme la présence d’introns interrompus conduisant à un épissage en trans. Ces séquence codantes peuvent être utilisées comme sondes pour caractériser le polymorphisme entre variétés ou espèces. La recherche de nouvelles séquences codantes utilisables nous a amenés à identifier et caractériser 2 gènes mt nouveaux, nad5 et nad6.
Tipo: Journal Article Palavras-chave: GENE NAD5; GENE NAD6; CONSERVATION DE SEQUENCE; RFLP; EPISSAGE EN TRANS; EDITION DES ARN; ADN MITOCHONDRIAL; GENOTHEQUE; SEQUENCE NUCLEOTIDIQUE; PLANTE; POLYMORPHISME GENETIQUE.
Ano: 1994 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9500023518052077&uri=/notices/prodinra1/2007/11/
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Construction of nested genetic core collections to optimize the exploitation of natural diversity in Vitis vinifera L. subsp sativa Inra
Le Cunff, L.; Fournier-Level, A.; Laucou, V.; Vezzulli, S.; Lacombe, T.; Adam-Blondon, A.F.; Boursiquot, J.M.; This, P..
Background: The first high quality draft of the grape genome sequence has just been published. This is a critical step in accessing all the genes of this species and increases the chances of exploiting the natural genetic diversity through association genetics. However, our basic knowledge of the extent of allelic variation within the species is still not sufficient. Towards this goal, we constructed nested genetic core collections (G-cores) to capture the simple sequence repeat (SSR) diversity of the grape cultivated compartment (Vitis vinifera L. subsp. sativa) from the world's largest germplasm collection (Domaine de Vassal, INRA Herault, France), containing 2262 unique genotypes. Results: Sub-samples of 12, 24, 48 and 92 varieties of V. vinifera L....
Tipo: Journal Article Palavras-chave: RESISTANCE AUX MALADIES; VIGNE; DIVERSITE; POLYMORPHISME; SEQUENCE NUCLEOTIDIQUE; MARQUEUR GENETIQUE; EXPRESSION DES GENES; DIVERSITE GENETIQUE SINGLE NUCLEOTIDE POLYMORPHISMS; LINKAGE DISEQUILIBRIUM; CULTIVATED GRAPEVINE; MICROSATELLITE LOCI; CROHNS DISEASE; WINE GRAPES; EXPRESSION; MUTATION; GENOME; SUSCEPTIBILITY.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20117dca1051&uri=/notices/prodinra1/2011/05/
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Distribution and chromosomal organization of 18S-5.8S-25S and 5S rDNA in Petunia species Inra
Benabdelmouna, A.; Abirached-Darmency, M..
Nous avons analysé la variation interspécifique de l’espaceur 5S ADNr par la tehnique d’amplification génique (PCR) dans sept espèces de Petunia. Les espèce étudiées peuvent être séparées en deux groupes suivant la taille des variants 5S ADNr amplifiés : un groupe avec une unité de répétition de 460 pb comprenant P linearis (2 n = 18) et P integrifolia (2 n = 14), et un deuxième groupe avec une unité de répétition de 350 pb comprenant toutes les autres espèces sauvages (2 n = 14) étudiées et les lignées de P hybrida. Les fragments amplifiés ont été clonés et ont servi de sondes pour l’hybridation in situ fluorescente afin de déterminer le nombre et la localisation des unités 5S. L’organisation chromosomique du 5S ADNr nous a permis de distinguer un groupe...
Tipo: Journal Article Palavras-chave: RFLP; CARTE GENETIQUE; SEQUENCE NUCLEOTIDIQUE.
Ano: 1997 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9800033102069818&uri=/notices/prodinra1/2010/02/
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Effect of lenght and location of heterologous sequences on RecA-mediated strand exchange Inra
Morel, P.; Stasiak, A.; Ehrlich, S.D.; Cassuto, E..
Tipo: Journal Article Palavras-chave: ECHANGE MOLECULAIRE; HETEROLOGUE; SEQUENCE NUCLEOTIDIQUE; RELATION STRUCTURE ACTIVITE; PROTEINE DE LIAISON.
Ano: 1994 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9500008193043694&uri=/notices/prodinra1/2010/09/
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