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Registros recuperados: 10
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Application of microsatellite markers for breeding and genetic conservation of herds of Pantaneiro sheep Electron. J. Biotechnol.
Crispim,Bruno do Amaral; Seno,Leonardo de Oliveira; Egito,Andréa Alves do; Vargas Junior,Fernando Miranda de; Grisolia,Alexéia Barufatti.
Background The aim of the present study was to assess the genetic diversity of Pantaneiro sheep, using microsatellite markers, in order to assist maintenance and management plans, enhance mating systems and reduce the inbreeding rate. A total of 127 animals were genotyped at eight microsatellite loci. They belonged to populations from the Experimental Farm of the Universidade Federal da Grande Dourados (UFGD) (Dourados/MS/Brazil) and Embrapa Pantanal (Corumbá/MS/Brazil). Results The population of Pantaneiro sheep from the UFGD exhibited a high mean number of alleles (11.13) and allelic richness (10.66). The polymorphic information content was highly informative in the locus studied, resulting in a mean value of 0.71. Observed heterozygosity was lower than...
Tipo: Journal article Palavras-chave: Animal genetic resources; Genetic diversity; Kinship; Locally adapted breed; STR.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600010
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Comparison of whole-genome amplifications for microsatellite genotyping of Rotylenchulus reniformis Electron. J. Biotechnol.
Arias,Renée S; Stetina,Salliana R; Scheffler,Brian E.
Currently, a large number of microsatellites are available for Rotylenchulus reniformis (reniform nematode); however, two barriers exist for genotyping samples from different geographical areas. The limited amount of nucleic acids obtained from single nematodes which would require their multiplication to obtain enough DNA for testing; and the strictly regulated transport of live samples and multiplication in greenhouse for being a plant pathogen. Whole-genome amplification (WGA) of samples consisting of one and five dead gravid females with their associated egg masses was successfully performed on disrupted tissue using three commercial kits. DNA yield after WGA ranged from 0.5 to 8 µg and was used to test 96 microsatellite markers we previously developed...
Tipo: Journal article Palavras-chave: Genotyping; Reniform nematode; SSR; STR; WGA.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300014
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Cross-species microsatellite amplification in South American Caimans (Caiman spp and Paleosuchus palpebrosus) Genet. Mol. Biol.
Zucoloto,Rodrigo Barban; Villela,Priscilla Marqui Schimidt; Verdade,Luciano Martins; Coutinho,Luiz Lehmann.
Microsatellite DNA markers have been used to assess genetic diversity and to study ecological behavioral characteristics in animals. Although these markers are powerful tools, their development is labor intensive and costly. Thus, before new markers are developed it is important to prospect the use of markers from related species. In the present study we investigated the possibility of using microsatellite markers developed for Alligator mississipiensis and Caiman latirostris in South American crocodilians. Our results demonstrate the use of microsatellite markers for Paleosuchus palpebrosus, Caiman crocodilus and Caiman yacare.
Tipo: Info:eu-repo/semantics/article Palavras-chave: SSR; STR; Primers; Crocodilians; Alligatorinae.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100015
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DATOS POBLACIONALES DE LOS MICROSATÉLITES DE ADN HUMANO D2S1338, D19S433, PENTA D, PENTA E Y SE-33 DE LA REGIÓN CENTRAL COLOMBIANA Acta biol.Colomb.
REY,MAURICIO; USAQUEN,WILLIAM; ACOSTA,EDWIN; PARRA,JULIO.
Los microsatélites o STR por su sigla en inglés (Short Tandem Repeat) son repeticiones de secuencias cortas de nucleótidos a través del DNA, de gran importancia debido a su carácter polimórfico que permite su utilización en la identificación genética de individuos o poblacionales. En este trabajo se investigaron los perfiles genéticos de una muestra de poblaciones humanas del altiplano cundiboyacense en Colombia; se usaron los marcadores STR's D2S1338, D19S433, PENTA D, PENTA E y SE-33. Éstos cinco STR's fueron amplificados mediante reacción en cadena de la polimerasa (PCR), corridos con electroforesis capilar, y tipificados con los programas Genscan y Genotyper. Como resultado se reportan las frecuencias alélicas de los cinco microsatélites (no reportados...
Tipo: Journal article Palavras-chave: STR; D2S1338; D19S433; PENTA D; PENTA E; SE-33.
Ano: 2009 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2009000200013
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Genetic structuring of Salminus hilarii Valenciennes, 1850 (Teleostei: Characiformes) in the rio Paraná basin as revealed by microsatellite and mitochondrial DNA markers Neotropical Ichthyology
Silva,Juliana Viana da; Hallerman,Eric M.; Orfão,Laura Helena; Hilsdorf,Alexandre Wagner Silva.
Genetic variation of Salminus hilarii was assessed by screening microsatellite loci and mitochondrial D-loop DNA across four sampling in the upper rio Paraná basin of Brazil. Genetic diversity - measured as mean expected heterozygosity (0.904) and mean number of alleles across populations (13.7) - was reasonably high. Differentiation of microsatellite allele frequencies among populations was shown to be low but significant by AMOVA Φ ST (0.0192), and high by D EST (0.185). D-loop variation was high, with haplotypic diversity of 0.950 and nucleotide diversity of 0.011. Mitochondrial DNA-based estimates for population differentiation were high, with an overall Φ ST of 0.173. The results of tests of nuclear and mitochondrial variation yielded no unequivocal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: D-loop; River disruption; STR; Tabarana.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000300547
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Microsatellite polymorphism in wheat from Brazilian cultivars; inter- and intra-varietal studies Genet. Mol. Biol.
Lima,Vitor Lopes de Abreu; Seki,Homiko Abreu; Rumjanek,Franklin David.
Eleven samples of wheat (Triticum aestivum) from different Brazilian cultivars and six American varieties were compared for polymorphism, using primers for nine different STR loci. STR analysis of DNA from single grains of the Brazilian varieties showed that for most loci there was very little intra-cultivar polymorphism. The polymorphic variation observed for Brazilian cultivars was similar to that seen in the American varieties. For the Brazilian cultivars PCR analysis could be performed on only one half of a grain. The American samples required more seeds for analysis. The nucleotide sequences of five amplified microsatellites selected at random from the Brazilian samples were also determined and compared to those of the Chinese Spring variety. Although...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wheat; Brazilian; Cultivars; DNA; STR; Polymorphism.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300021
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Microsatellite variation and population genetic structure of a neotropical endangered Bryconinae species Brycon insignis Steindachner, 1877: implications for its conservation and sustainable management Neotropical Ichthyology
Matsumoto,Cristianne Kayoko; Hilsdorf,Alexandre Wagner Silva.
Piabanha (Brycon insignis) is a freshwater fish species from the drainages in Southeastern Brazil. During the 1950s, it was an important economic and food resource for local populations, but dramatic and continuous environmental degradation seriously jeopardized the B. insignis populations in the region. Microsatellite markers were used to assess the genetic structure of wild populations of B. insignis and compare the genetic variability and integrity of the wild populations with a captive population. Samples of DNA from 208 specimens from geographically isolated populations were analyzed. Population genetic structure was investigated using F ST, R ST estimates as well as AMOVA. All five loci used in this study were polymorphic with observed heterozygosity...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Piabanha; Paraíba do Sul basin; Conservation; STR; Fish.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252009000300006
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Mutation rate of 7 X-STRs of common use in population genetics JBAG
Glesmann,Laura A.; Martina,Pablo F.; Vidal Rioja,Lidia; Catanesi,Cecilia I..
Microsatellite loci analysis is a relevant tool in population genetic studies and also in paternity testing. In this field the X-chromosome STR loci are widely used for female offspring testing but their mutation rates are scarcely known. In this work we performed a mutation rate analysis of seven STR markers located in the X- chromosome specific region, in mother-son pairs and father-mother-daughter trios. Among 1015 allele transfers, we found only one change for a mother-daughter transmission in the DXS9898 locus, consisting of a loss of one single repeat. This finding allowed us to estimate a general mutation rate of μ=9.85 x 10-4 (2.49 x 10-4 - 5.49 x 10-2) which can be applied to population studies. This value is consistent with other reports...
Tipo: Info:eu-repo/semantics/article Palavras-chave: X chromosome; STR; Mutation rate; Population genetics.
Ano: 2009 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332009000200002
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The use of microsatellite markers in Neotropical studies of wild birds: a literature review Anais da ABC (AABC)
MOURA,RENAN F.; DAWSON,DEBORAH A.; NOGUEIRA,DENISE M..
ABSTRACT Despite extensive habitat fragmentation, the Neotropical region possesses 30% of the world´s bird species. Microsatellites have remained one of the most popular genetic markers and have been used in ecological and conservation studies since the 1990’s. We conducted a literature review comparing the number of papers published from January 1990 to July 2015 that used microsatellite markers for studies of wild birds in the Neotropical region, USA and some European countries. We assigned the articles to three categories of studies: population genetics, animal behavior/kinship analysis and the development of species-specific bird microsatellite markers. We also compared the studies in the Neotropics that used heterologous versus species-specific...
Tipo: Info:eu-repo/semantics/article Palavras-chave: AVES; Cross-species amplification; Biodiversity; Ecology; Molecular markers; STR.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000100145
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Y Chromosomal STR Polymorphism in Northern Chinese Populations Biol. Res.
ZHENG,LIHONG; SUN,HAIMING; WANG,JINGWEI; LI,SHILIN; BAI,JING; JIN,YAN; YU,YANG; CHEN,FENG; JIN,LI; FU,SONGBIN.
Y chromosomal STRs show sufficient variability among individduals in a population and a high degree of geographical differentiation, such that their polymorphic character makes them especially suited for population genetic studies. To investígate the polymorphism of a set of 17 Y-STR loci in northern China, we genotyped the 17 Y chromosomal STR loci in a population sample of 377 unrelated males from eight ethnic populations in northern China. We calculated the haplotype frequencies, Rst value and carried out the analysis of molecular variance (AMOVA). We then drew the multidimensional scaling analysis (MDS) plot and phylogenetic tree based on the Rst value. All populations showed a high level of haplotype diversity, with low inter-population variance as...
Tipo: Journal article Palavras-chave: China; Population; STR; Y chromosome.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400011
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