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Registros recuperados: 38
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A study on the genetic diversity of Androctonus crassicauda (Olivier, 1807; Scorpiones: Buthidae) from Turkey J. Venom. Anim. Toxins incl. Trop. Dis.
Ozkan,O; Ahmet,C; Zafer,K.
Mitochondrial DNA (mtDNA) has been widely employed in phylogeographic and phylogenetic studies. In the present study, the genetic identification of the scorpion Androctonus crassicauda (Olivier, 1807) was carried out by using the 16S mitochondrial gene, since this scorpion represents the most important species in Turkey regarding scorpionism and antivenom production. Two genetic groups were found according to the sequence analysis results, while five different loci at the nucleotide level presented genetic variation in the 16S region when compared to a known A. crassicauda sequence data (GenBank, AJ277598). Nucleotide variations found in the current work constitute the first descriptive report for A. crassicauda. Moreover, future studies may enlighten the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Scorpion; Androctonus crassicauda; PCR; Genotype; Turkey.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400010
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Anticoagulant and factor Xa-like activities of Tityus discrepans scorpion venom Acta Toxicol. Argent.
Brazón,Josmary; Guerrero,Belsy; Suze,D; Sevcik, Carlos,Gina; Arocha-Piñango,Carmen L.
Tityus discrepans venom (TdV) produces a variety of haemostatic manifestations including alveoli fbrin deposition and/ or prothrombin and partial thromboplastin time (PT, PTT) alterations in mammals. In vitro studies have demonstrated that TdV contains tissue plasminogen activator-like (t-PA), fbrinolytic and plasmin inhibitory compounds and produces platelets activation through GPVI and a novel Src-dependent signalling pathway. The aim of this study is to describe the initial characterization of procoagulant and anticoagulant components from TdV. This venom was fractionated by exclusion molecular chromatography on a Sephadex G-50 column. The eluted material was collected as fve fractions called S1 to S5. These fractions and the whole venom were used to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Scorpion; Coagulation; Fibrinogen; Factor Xa.
Ano: 2013 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-37432013000100003
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Arthropod venom Hyaluronidases: biochemical properties and potential applications in medicine and biotechnology J. Venom. Anim. Toxins incl. Trop. Dis.
Bordon,Karla C F; Wiezel,Gisele A.; Amorim,Fernanda G.; Arantes,Eliane C..
AbstractHyaluronidases are enzymes that mainly degrade hyaluronan, the major glycosaminoglycan of the interstitial matrix. They are involved in several pathological and physiological activities including fertilization, wound healing, embryogenesis, angiogenesis, diffusion of toxins and drugs, metastasis, pneumonia, sepsis, bacteremia, meningitis, inflammation and allergy, among others. Hyaluronidases are widely distributed in nature and the enzymes from mammalian spermatozoa, lysosomes and animal venoms belong to the subclass EC 3.2.1.35. To date, only five three-dimensional structures for arthropod venom hyaluronidases (Apis mellifera and Vespula vulgaris) were determined. Additionally, there are four molecular models for hyaluronidases fromMesobuthus...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hyaluronidase; Scorpion; Spider; Caterpillar; Hymenoptera; Insects; Cloning; Heterologous expression; PEGylation; Biotechnological applications.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100214
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Cleaning behaviors in four scorpion species J. Venom. Anim. Toxins incl. Trop. Dis.
Jiao,GB; Zhu,MS.
Scorpions rely predominantly on mechanosensory and chemosensory organs to guide their orientation behaviors. Once sensory organs are affected by the presence of dirt such as clay or prey bodily fluid, scorpions may display a cleaning behavior to reduce or eliminate its influence on their sensory capabilities. In the laboratory, cleaning behaviors of two buthid species, Mesobuthus eupeus (Koch, 1839) and Mesobuthus caucasicus (Nordmann, 1840), and one euscorpiid species, Scorpiops luridus Zhu Lourenço & Qi, 2005 from China, were observed before and after feeding. Moreover, two distinct cleaning behaviors in Scorpiops luridus and three in Heterometrus petersii (Thorell, 1876) (Scorpionidae) were noted for several times during daily activities. Based...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Scorpion; Cleaning behavior; Cleaning means; Prey capture; Daily activity.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200017
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Clinical and cardiovascular alterations produced by scorpion envenomation in dogs J. Venom. Anim. Toxins incl. Trop. Dis.
Cordeiro,F. F.; Sakate,M.; Fernandes,V.; Cuyumjian,P. R..
Scorpionism is a common problem that occurs in tropical and subtropical countries and assumes great medical-sanitary importance due to its fatal effect on sensitive individuals, being able to lead children and aged people to death. The envenomation lethal potential is responsible for the serious cardiopulmonary alterations the scorpion toxin produces in its victims. The present research evaluated the effects of Tityus serrulatus venom on dogs, using two distinct doses: a dose that simulates natural envenomation (0.4 mg/total dose), and an experimental dose (0.25 mg/kg). General clinical signs were observed at different moments after envenomation, and specific data related to the cardiopulmonary system were evaluated by systemic arterial pressure...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Scorpion; Dogs; Cardiopulmonary alterations; Tityus serrulatus.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000100003
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Courtship and mating of Scorpiops luridus Zhu Lourenço & Qi, 2005 (Scorpiones: Euscorpiidae) from Xizang province, China J. Venom. Anim. Toxins incl. Trop. Dis.
Jiao,GB; Zhu,MS.
In the current work, the courtship and mating of Scorpiops luridus Zhu Lourenço & Qi, 2005 (Euscorpiidae) from Xizang province (Tibet), China, were studied for the first time in the laboratory. Most of the mating behaviors in Scorpiops luridus are not remarkably different from those exhibited by other scorpions. However, for the first time a male pulling a female with its chelicerae to rapidly accomplish the sperm uptake was observed. Additionally, the sexual stinging behavior displayed by the male occurred in the initial stage, not during the promenade stage as previously described in several scorpion species. Through observation and analysis, we speculate that venom injection during sexual stinging is selective, possibly relying on the status...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Scorpion; Scorpiops; Courtship; Mating; Behavior.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100015
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Determination of potency and paraspecific effects of Androctonus crassicauda (Olivier, 1807) antivenom against Mesobuthus gibbosus (Brullé, 1832) venom (Scorpiones: Buthidae) J. Venom. Anim. Toxins incl. Trop. Dis.
Ozkan,O.; Adiguzel,S.; Kar,S.; Yakistiran,S.; Cesaretli,Y.; Karaer,K. Z..
Scorpion envenomation remains a real health problem in many countries. In scorpionism cases, it is often recommended that patients be treated with species-specific antivenom. Androctonus crassicauda venom has been used as antigen for antivenom production in Turkey, where this antivenom, called Turkish antivenom, has also been effective in the treatment of envenomation caused by species other than A. crassicauda. The present study aimed at determining the paraspecific effects and potency of the Turkish antivenom against Mesobuthus gibbosus (Brullé, 1832) venom. To assess the venom toxicity and the antivenom efficacy, we determined the Minimum Lethal Dose (MLD) and the Minimum Effective Dose (MED) instead of LD50 and ED50, respectively. Androctonus...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Scorpion; Antivenom; Androctonus crassicauda; Mesobuthus gibbosus; Paraspecific activity.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000200008
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Divergences between the Brazilian national information systems for recording deaths from venomous animals J. Venom. Anim. Toxins incl. Trop. Dis.
Bochner,Rosany; Souza,Claudio Mauricio Vieira de.
Abstract Background: This paper aims to highlight and analyze discrepancies in reporting of deaths due to venomous animals in Brazil, from 2001 to 2015, between two national information systems: The Notifiable Diseases Information System (Sistema de Informação de Agravos de Notificação - SINAN) and the Mortality Information System (Sistema de Informações sobre Mortalidade - SIM). Methods: Descriptive and comparative study of the SINAN and SIM information systems, was conducted via the following steps: collecting the death notices from SINAN and SIM; constructing tables and comparative graphics; and, only in scorpion sting fatalities, analyzing the distribution of deaths by age group as described in the specialized literature. Results: While SINAN...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Information system; Death; SINAN; SIM; Snake; Spider; Scorpion; Bee.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100313
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Effects of Androctonus crassicauda (Olivier, 1807) (scorpiones: buthidae) venom on rats: correlation among acetylcholinesterase activities and electrolytes levels J. Venom. Anim. Toxins incl. Trop. Dis.
Ozkan,O.; Adiguzel,S.; Kar,S.; Kurt,M.; Yakistiran,S.; Cesaretli,Y.; Orman,M.; Karaer,Z..
Scorpions can be considered living fossils because they have changed so little during the last 400 million years. They are venomous arthropods of the Arachnida class and regarded as relatives of spiders, ticks and mites. The aim of the present study was to evaluate the toxicity of Androctonus crassicauda (Olivier, 1807) venom and its effects on the acetylcholinesterase (AchE) activity and on electrolytes levels in rats. Animals were divided into seven groups of five rats each. Test groups received 250µg/kg of venom solution while control group was treated with 200µl of physiological saline solution (PSS). Blood samples were collected from the animals on the 1st, 2nd 4th, 8th, 12th, and 24th hours after subcutaneous injection of venom. Animals were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Scorpion; Androctonus crassicauda; Venom; Acetylcholinesterase; Electrolytes.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000100005
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Envenomation by scorpion in dog: case report J. Venom. Anim. Toxins incl. Trop. Dis.
Cardoso,M. J. L.; Sakate,M.; Ciampolini,P.; Moutinho,F. Q.; Cherubini,A. L..
A case admitted at the Small Animals Clinics Service, Veterinary Hospital, FMVZ - UNESP, in May, 1999 is described. A Brazilian Terrier dog, 3 years and 3 months old, weighing 1.7 kg was brought after 2 hours and a half from contact with a scorpion (Tityus bahiensis). The dog showed vocalization inserted with drowsiness. Physical examination showed slightly hyperemic mucous, pain, agressiveness, tachypnea, tachycardia, and discrete erythema on the right forelimb palmar face. An anesthetic block was performed around the stung area using 2% lidocaine hydrochloride without vasoconstrictor (10mL). The animal was asymptomatic, after 24 h treatment.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Scorpion; Dog; Envenomation; Poisoning; Tityus bahiensis.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000100008
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Euscorpius (Scorpiones, Euscorpiidae): three cases of stings in northwestern Italy J. Venom. Anim. Toxins incl. Trop. Dis.
Dutto,M; Dutto,L; Scaglione,N; Bertero,M.
In the period between June 2008 and August 2009, three cases of stings of Euscorpius scorpions indigenous to Italy were treated at two different emergency departments (ED) in hospitals of the Piedmont region, northwest Italy: Santa Croce e Carle General Hospital in Cuneo, and Santissima Annunziata Hospital in Savigliano. Scorpion stings in Italy are rare and not well documented in the literature; this situation may raise doubts among medical personnel as to how such lesions are best treated. Analysis of the incidents confirms that the venom of Euscorpius do not provokes systemic poisoning in humans and in these cases even dermatological reactions were not significant.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Euscorpius; Scorpion; Sting; Scorpionism; Venom; Poisoning; Italy.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400018
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Evaluation of the neutralizing capacity of Androctonus crassicauda (Olivier, 1807) antivenom against Leiurus quinquestriatus (Ehrenberg, 1928) venom (Scorpiones: Buthidae) J. Venom. Anim. Toxins incl. Trop. Dis.
Ozkan,O; Adiguzel,S; Inceoglu,B; Lango,J; Ertek,M; Hammock,BD.
The two most venomous species of the family Buthidae, Leiurus quinquestriatus and Androctonus crassicauda, are found in Africa and in the Middle East. Potency and paraspecific activities of A. crassicauda antivenom (RSHC anti-Ac) were tested against L. quinquestriatus venom. The sera produced by Refik Saydam Hygiene Center (RSHC) showed strong reactivity against the venoms of A. crassicauda and L. quinquestriatus in western blotting and dot-blot analysis. RSHC anti-Ac presents immunoactivity and neutralizing potential against Leiurus quinquestriatus venom. Neutralization capacity of antivenom was found to be 400 µL against 40 minimum lethal doses (MLD) of A. crassicauda scorpion venom and 10 MLD of L. quinquestriatus venom. This study indicates that the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Scorpion; Androctonus crassicauda; Leiurus quinquestriatus; Venom; Monovalent antivenom; Paraspecific activity.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300008
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Fine structural analysis of the stinger in venom apparatus of the scorpion Euscorpius mingrelicus (Scorpiones: Euscorpiidae) J. Venom. Anim. Toxins incl. Trop. Dis.
Yigit,N; Benli,M.
In this study, the morphology, histology and fine structure of the stinger, a part of the venom apparatus of Euscorpius mingrelicus (Kessler, 1874) (Scorpiones: Euscorpiidae) were studied by light microscopy and transmission electron microscopy (TEM). The stinger, located at the end section of the telson, is sickle-shaped. The venom is ejected through a pair of venom pores on its subterminal portion. Both venom ducts extend along the stinger without contact with each other since they are separated by connective tissue cells. The stinger cuticle is composed of two layers. Additionally, there are many pore canals and some hemolymph vessels in the cuticle. This work constitutes the first histological and fine structure study on Euscorpius mingrelicus stinger.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Scorpion; Euscorpius mingrelicus; Telson; Stinger; Fine structure; Transmission electron microscopy.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100008
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Hematological and biochemical profiles of rats submitted to experimental poisoning with Tityus serrulatus venom Arq. Bras. Med. Vet. Zootec.
Pinto,M.C.L.; Melo,M.M.; Costa,M.E.R.; Labarrere,C.R..
The hematological and biochemical profiles of newly weaned rats submitted to experimental poisoning with T. serrulatus venom were evaluated. Fifteen recently weaned male Wistar rats (mean weight 130g) were distributed into three equal groups (n = 5). Animals in the control group (group A) received a subcutaneous injection of 400μL of ultra-pure water, while those in the experimental groups received, by identical route, 400μL of a solution containing 100μg (group B) or 450μg (group C) of scorpion venom dissolved in ultra-pure water. Red blood cells indexes, and differential leukocyte and total platelet counts were determined, together with levels of serum glucose, urea, creatinine, lactic dehydrogenase, aspartate aminotransferase, amylase, insulin, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rat; Tityus serrulatus; Scorpion; Experimental envenoming; Hematology; Biochemestry.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000200015
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Histology and histochemistry of the venom apparatus of the black scorpion Androctonus crassicauda (Olivier, 1807) (Scorpiones: Buthidae) J. Venom. Anim. Toxins incl. Trop. Dis.
Jarrar,BM; Al-Rowaily,MA.
The venom apparatus of the black scorpion Androctonus crassicauda has been characterized histologically and histochemically in the present study. The results showed that this apparatus consists of paired venom glands, each of which initially presents its own canal and posteriorily both fuse into a single common one. Each gland is covered by a sheath of striated muscle and is lined with extensively folded secretory epithelium (formed of non-secretory and secretory venom-producing cells). The outcomes also revealed that the venom-producing cells of both glands produce neutral mucosubstances, sialomucins, sulfomucins and proteins, but are devoid of glycogen. Cysteine, tyrosine, tryptophan and arginine were also detected along with activities of acid and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Androctonus; Scorpion; Venom apparatus; Histochemistry; Phosphatases; Esterases; Peptidases.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300010
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Histopathological changes in albino mouse testis induced by the administration of Tityus n. sp. venom J. Venom. Anim. Toxins
PENNA-VIDEAÚ,S.; CERMEÑO-VIVAS,J.; MORENO-MARVAL,M.; QUIROGA-NOTTI,M..
Scorpion envenoming is a public health concern in northeastern Venezuela. Specimens of the genus Tityus are responsible for most of these. In experimental animals, Tityus venom produces histopathological changes in the skeletal muscle and pancreas, but its toxicity to the reproductive system has not been studied. The aim of this work is to describe the histopathological changes in testis and epididymis of albino mice induced by the administration of Tityus n. sp. venom. Sub-lethal doses of venom (3.75 mg/g mouse) were administered intramuscularly (IM) daily for 4 days. On the fifth day, the animals were sacrificed and the testes and epididymes were quickly removed and processed for light microscopy. The venom induced alterations in spermatogenesis. Sertoli...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Testis; Sertoli cell; Epididymis; Scorpion; Venom; Mouse; Histopathology.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302000000200003
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In vivo pharmacological study on the effectiveness of available polyclonal antivenom against Hemiscorpius lepturus venom J. Venom. Anim. Toxins incl. Trop. Dis.
Jalali,A; Pipelzadeh,M H; Seyedian,R; Rahmani,A H; Omidian,N.
The available Razi Institute antivenom is still, empirically, used by intramuscular (IM) administration for the treatment of scorpion stings in humans by six medically dangerous species including Hemiscorpius lepturus (H. lepturus). The aim of this study was to assess the neutralizing ability and effectiveness of the antivenom in inhibiting hemoglobinuria, biochemical changes, increased microalbuminuria and urinary lactate dehydrogenase (LDH) following H. lepturus sting. Simultaneous intramuscular administration of 10 μL and 100 μL of antivenom, after 24 hours, had no significant preventive effect on the extent and degree of hemoglobinuria or proteinuria produced in venom-treated rats. After IM administration of antivenom, no significant changes in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hemiscorpius lepturus; Scorpion; Venom; Hemolysis; Antivenom.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200004
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Incidence and severity of scorpion stings in Algeria J. Venom. Anim. Toxins incl. Trop. Dis.
Laïd,Y; Boutekdjiret,L; Oudjehane,R; Laraba-Djebari,F; Hellal,H; Guerinik,M; Griene,L; Alamir,B; Merad,R; Chippaux,JP.
Scorpion stings are a public health problem in the Maghreb region. In Algeria, epidemiological data were collected over the past twenty years by the Algerian health authorities. This study is an analysis of morbidity and mortality data collected from 2001 to 2010. Annual incidence and mortality due to scorpion envenoming were 152 ± 3.6 stings and 0.236 ± 0.041 deaths per 100,000 people (95% CI), respectively. The risk of being stung by a scorpion was dramatically higher in southern areas and central highlands due to environmental conditions. Incidence of envenoming was especially higher in the adult population, and among young males. In contrast, mortality was significantly higher among children under 15 years, particularly ages 1-4. Upper limbs were more...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Scorpion; Stings; Envenoming; Deaths; Epidemiology; Management; Algeria.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400008
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Individual variation in the protein profile of the venom of Mesobuthus gibbosus (Brullé, 1832, Scorpiones: Buthidae) from Turkey J. Venom. Anim. Toxins incl. Trop. Dis.
Ozkan,O; Ciftci,G.
Mesobuthus gibbosus (Brullé, 1832) scorpions were collected from the Mugla province in the Aegean region of Turkey and housed in individual boxes. After extraction, the venom composition was analyzed using gel electrophoresis (SDS-PAGE). Interestingly, all scorpion venom samples contained only one protein band (~68 kDa) in common. Two protein bands (30 and 98 kDa) were common in six venom samples and were absent in the other venoms. Furthermore, two different protein bands (28 and 45 kDa) were detected in seven venom samples. This study proposes possible variations in the composition of individual scorpion venom samples collected from the same geographic region, based on the electrophoretic profile. Additional studies will be necessary in order to assess...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Scorpion; Mesobuthus gibbosus; Venom; Protein; Turkey.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300017
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INSULIN ADMINISTRATION IN SEVERE SCORPION ENVENOMING J. Venom. Anim. Toxins
YUGANDHAR,B.; RADHA KRISHNA MURTHY,K.; SATTAR,S. A..
The efficacy of insulin-glucose infusion in reversing myocardial damage, haemodynamic changes, peripheral circulatory failure, and pulmonary oedema was evaluated in 25 victims of venomous scorpion stings from the Rayalaseema region in the south of India. Myocardial damage with peripheral circulatory failure was seen in all scorpion sting victims. Ten of these victims also had pulmonary oedema. All the patients received continuous infusion of regular crystalline insulin at the rate of 0.3 U/g of glucose and glucose at the rate of 0.1 g/kg/h with supplementary potassium as needed, inotropic agents, oxygen, as well as maintenance of fluid, electrolytes and acid-base balance. Insulin-glucose infusion was associated with reversal of cardiovascular and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Scorpion; Myocardial damage; Pulmonary oedema; Insulin-glucose infusion; Peripheral circulatory failure.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301999000200007
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