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Ectonucleotidase activities in Sertoli cells from immature rats BJMBR
Casali,E.A.; Silva,T.R. da; Gelain,D.P.; Kaiser,G.R.R.F.; Battastini,A.M.O.; Sarkis,J.J.F.; Bernard,E.A..
Sertoli cells have been shown to be targets for extracellular purines such as ATP and adenosine. These purines evoke responses in Sertoli cells through two subtypes of purinoreceptors, P2Y2 and P A1. The signals to purinoreceptors are usually terminated by the action of ectonucleotidases. To demonstrate these enzymatic activities, we cultured rat Sertoli cells for four days and then used them for different assays. ATP, ADP and AMP hydrolysis was estimated by measuring the Pi released using a colorimetric method. Adenosine deaminase activity (EC 3.5.4.4) was determined by HPLC. The cells were not disrupted after 40 min of incubation and the enzymatic activities were considered to be ectocellularly localized. ATP and ADP hydrolysis was markedly increased by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sertoli cells; Purine nucleotides; ATP diphosphohydrolase; Ecto-5'-nucleotidase; Ectoadenosine deaminase.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000003
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Effect of FSH and insulin on lipogenesis in cultures of Sertoli cells from immature rats BJMBR
Follicle-stimulating hormone (FSH) and insulin regulate glycide metabolism in Sertoli cells, thus stimulating lactate production. These stimulatory effects of FSH and insulin do not require protein synthesis, suggesting a modulation of enzyme activity and/or regulation of glucose transport. The present investigation was performed to characterize the hormonal control of lipid metabolism in Sertoli cells. The data indicate that FSH and insulin have a regulatory effect on lipid metabolism in Sertoli cells. After 8 h of preincubation with insulin (5 µg/ml), the activity of the enzyme ATP-citrate lyase in cultured Sertoli cells was increased from 0.19 to 0.34 nmol NAD+ formed µg protein-1 min-1. FSH (100 ng/ml) had no effect on this enzyme. Glycerol phosphate...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Sertoli cells; Lipids; FSH; Insulin.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500004
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Morphometry and histomorphometry of the testis in crossbred pigs fed diets with different protein levels Arq. Bras. Med. Vet. Zootec.
Valença,R.M.B.; Silva Junior,V.A.; Araújo,L.P.C.; Reis,J.C.; Guerra,M.M.P.; Soares,P.C.; Costa,A.N..
Aiming to evaluate the effect of the diet protein content on testicular parameters in pigs, 21 non-gelded male Dalland pigs were used and randomly divided into three groups. Males belonging to groups G2 and G3 received a diet with crude protein levels of 15% below and above, respectively, in relation to G1 (control). At 210 days of age, animals were castrated, and testis and epididymis were collected for morphometric and histomorphometry analyses. No difference was observed in relation to the total length of seminiferous tubules (G1=3239.9±333,3m; G2=2989.4±171,7m and G3=3059.5±254.9m), population of Sertoli cell (G1=4.7±0.5x10(9); G2=4.3±0.3x10(9) and G3=4.7±0.5x10(9)), population (G1=31.6±5.58x10(9); G2=27.3±4.0x10(9) and G3=26.4±3.9x10(9)) and volume of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pigs; Sperm production; Sertoli cells; Protein; Testosterone.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000500010
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Neonatal Administration of Fluoxetine Decreased Final Sertoli Cell Number in Wistar Rats International Journal of Morphology
Silva Junior,Valdemiro Amaro da; Lins Amorim,Marleyne José Afonso Accioly; Amorim Junior,Adelmar Afonso de; Pinto,Catarina Ferreira; Deiró,Tereza B. J.; Oliveira,João Ricardo M. de; Peixoto,Christina Alves; Manhães-de-Castro,Raul.
The aim of the present study was to test the hypothesis that the application of fluoxetine ­ a highly selective serotonin reuptake inhibitor (SSRI) ­ in rats during the suckling period induces changes in testicular development. Groups of newborn male rats were randomly assigned with different doses of fluoxetine 24 hours after birth. Each litter stayed with its respective mother during 21 days. Body weight (BW) was measured daily from the 1st -21st day to calculate daily doses of fluoxetine. 5 mg (T1), 10 mg (T2) 20 mg (T3) or deionized water, were injected intraperitoneally. On the 21st day, animals were heparinized, anesthetized and blood was collected by cardiac puncture to determine by radioimmunoassay the follicle stimulating hormone (FSH) levels....
Tipo: Journal article Palavras-chave: Fluoxetine; Testis development; Sertoli cells; Wistar rats.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000100009
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Retinol-induced changes in the phosphorylation levels of histones and high mobility group proteins from Sertoli cells BJMBR
Moreira,J.C.F.; Dal-Pizzol,F.; Rocha,A.B.; Klamt,F.; Ribeiro,N.C.; Ferreira,C.J.S.; Bernard,E.A..
Chromatin proteins play a role in the organization and functions of DNA. Covalent modifications of nuclear proteins modulate their interactions with DNA sequences and are probably one of the multiple factors involved in the process of switch on/off transcriptionally active regions of DNA. Histones and high mobility group proteins (HMG) are subject to many covalent modifications that may modulate their capacity to bind to DNA. We investigated the changes induced in the phosphorylation pattern of cultured Wistar rat Sertoli cell histones and high mobility group protein subfamilies exposed to 7 µM retinol for up to 48 h. In each experiment, 6 h before the end of the retinol treatment each culture flask received 370 KBq/ml [32P]-phosphate. The histone and HMGs...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sertoli cells; Phosphorylation; Histones; High mobility group proteins; Retinol.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300005
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Vitamin C and oxidative stress in the seminiferous epithelium Biol. Res.
Angulo,Constanza; Maldonado,Rodrigo; Pulgar,Eduardo; Mancilla,Héctor; Córdova,Alex; Villarroel,Franz; Castro,Maite A; Concha,Ilona I.
In this article, we focus on the fundamental role of vitamin C transporters for the normal delivery of vitamin C to germ cells in the adluminal compartment of seminiferous tubules. We argue that the redox status within spermatozoa or in semen is partly responsible for the etiology of infertility. In this context, antioxidant defence plays a critical role in male fertility. Vitamin C, a micronutrient required for a wide variety of metabolic functions, has long been associated with male reproduction. Two systems for vitamin C transport have been described in mammals. Facilitative hexose transporters (GLUTs), with 14 known isoforms to date, GLUT1-GLUT14, transport the oxidized form of vitamin C (dehydroascorbic acid) into the cells. Sodium ascorbic acid...
Tipo: Journal article Palavras-chave: Ascorbic acid; Oxidative stress; GLUT; Sertoli cells; SVCT; Vitamin C transporters.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200009
Registros recuperados: 6
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