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Registros recuperados: 10
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A novel multiplex PCR for the simultaneous detection of Salmonella enterica and Shigella species BJM
Radhika,M.; Saugata,Majumder; Murali,H.S.; Batra,H.V..
Salmonella enterica and Shigella species are commonly associated with food and water borne infections leading to gastrointestinal diseases. The present work was undertaken to develop a sensitive and reliable PCR based detection system for simultaneous detection of Salmonella enterica and Shigella at species level. For this the conserved regions of specific genes namely ipaH1, ipaH, wbgZ, wzy and invA were targeted for detection of Shigella genus, S. flexneri, S. sonnei, S. boydii and Salmonella enterica respectively along with an internal amplification control (IAC). The results showed that twenty Salmonella and eleven Shigella spp., were accurately identified by the assay without showing non-specificity against closely related other Enterobacteriaceae...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella; Shigella; Multiplex PCR; Spiking.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200041
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Aislamiento, filogenia e identificación bioquímica de una especie de Shigella de rumen con capacidad para degradar celulosa. Colegio de Postgraduados
Luna Rodríguez, Lorena.
De una muestra tomada del rumen de un bovino se aisló una cepa de Shigella sp nov en condiciones anaerobias, y creció en medio selectivo cuya única fuente de carbono fue celulosa, a 39 °C y pH 6.8. La bacteria aislada es Gram negativa, en forma de coco bacilo, con actividad celulolítica cuando se utilizó la tinción con rojo congo en colonias de 10 d. Los resultados filogenéticos indican que se encuentra ubicada en el grupo monofilético perteneciente al género Shigella, pero con 98 % de similitud con respecto a las demás especies del taxa, por lo cual este microorganismo es una especie no reconocida. La utilización de azúcares y alcoholes de la cepa aislada se determinó con el Kit API 50 CH y el Software API WEB. De acuerdo con los resultados hubo...
Palavras-chave: Shigella; Rumen; Celulolítica; Monofilético; Cellulolytic; Monophyletic; Ganadería; Doctorado.
Ano: 2013 URL: http://hdl.handle.net/10521/1860
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Antimicrobial resistance and PCR-ribotyping of Shigella responsible for foodborne outbreaks occurred in southern Brazil BJM
Paula,Cheila Minéia Daniel de; Geimba,Mercedes Passos; Amaral,Patrícia Heidrich do; Tondo,Eduardo Cesar.
Little information about Shigella responsible for foodborne shigellosis is available in Brazil. The present study aimed to investigate the antimicrobial resistance and PCR-ribotyping patterns of Shigella isolates responsible for foodborne outbreaks occurred in Rio Grande do Sul State (RS), Southern Brazil in the period between 2003 and 2007. Shigella strains (n=152) were isolated from foods and fecal samples of victims of shigellosis outbreaks investigated by the Surveillance Service. Identification of the strains at specie level indicated that 71.1% of them were S. flexneri, 21.5% S. sonnei, and 0.7% S. dysenteriae. Ten strains (6.7%) were identified only as Shigella spp. An increasing occurrence of S. sonnei was observed after 2004. Most of the strains...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Shigellosis; Shigella; Antimicrobial; PCR-Ribotyping; RS/Brazil.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400015
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Detection of shigella in lettuce by the use of a rapid molecular assay with increased sensitivity BJM
Jiménez,Kenia Barrantes; McCoy,Clyde B.; Achí,Rosario.
A Multiplex Polymerase Chain Reaction (PCR) assay to be used as an alternative to the conventional culture method in detecting Shigella and enteroinvasive Escherichia coli (EIEC) virulence genes ipaH and ial in lettuce was developed. Efficacy and rapidity of the molecular method were determined as compared to the conventional culture. Lettuce samples were inoculated with different Shigella flexneri concentrations (from 10 CFU/ml to 10(7) CFU/ml). DNA was extracted directly from lettuce after inoculation (direct-PCR) and after an enrichment step (enrichment PCR). Multiplex PCR detection limit was 10(4) CFU/ml, diagnostic sensitivity and specificity were 100% accurate. An internal amplification control (IAC) of 100 bp was used in order to avoid false...
Tipo: Info:eu-repo/semantics/article Palavras-chave: PCR; Shigella; Rapid method; Lettuce; Food.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400018
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Development of multiplex loop-mediated isothermal amplification for three foodborne pathogens Ciênc. Tecnol. Aliment.
XU,Cong; LUO,Hongbin; ZHANG,Yun.
Abstract Staphylococcus aureus, Salmonella, and Shigella are three major foodborne pathogenic microorganisms that cause global public health problems. We developed a multiplex loop-mediated isothermal amplification (mLAMP) assay for simultaneous detection of S. aureus nuc, Salmonella fimY, and Shigella ipaH in fresh fruit juice using three sets of primers. In addition, three different restriction enzyme cleavage sites were designed in each forward inner primer (FIP), namely, XhoI in nuc FIP, KpnI in fimY FIP, and BamHI in ipaH FIP. DNA was amplified using the LAMP assay at 64 °C for 50 min followed by endonuclease restriction digestion to separate the LAMP products of three pathogens. The minimum amount of genomic DNA of S. aureus, Salmonella, and Shigella...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Multiplex LAMP; Staphylococcus aureus; Salmonella; Shigella.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019005028103
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Enterobacteriaceae in mouth and cloaca of podocnemis expansa and P. Unifilis (testudines: chelonia) populations of national park of araguaia plains, Brazil BJM
Morais,Paula Benevides de; Souza,Denise Rodrigues de; Sousa,Francisca Maria Pinheiro de; Oliveira,Kleverson Wessel de; Pimenta,Raphael Sanzio.
Shigella flexnerii and Escherichia coli were the most frequent Gram-negative bacteria found in the mouth cavity and cloacae of the turtles Podocnemis expansa and P. unifilis on beaches in the National Park of Araguaia, Brazil. Reptiles are known as Salmonella carriers, despite rarely isolated in these turtles.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella; Shigella; Enterobacteriaceae; Turtles; Bananal Island.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200017
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Frequency and antimicrobial susceptibility of Shigella species isolated in Children Medical Center Hospital, Tehran, Iran, 2001-2006 BJID
Pourakbari,Babak; Mamishi,Setareh; Mashoori,Negar; Mahboobi,Nastaran; Ashtiani,Mohammad H; Afsharpaiman,Shahla; Abedini,Masomeh.
Appropriate antimicrobial treatment of shigellosis depends on identifying its changing resistance pattern over time. We evaluated 15,255 stool culture submitted from July 2001 to June 2006 to the Laboratory of Children Medical Center Hospital. Specimen culture, bacterial identification, and disk diffusion susceptibility testing were performed according to National Committee for Clinical Laboratory Standards guidelines. From 15,255 stool samples, 682 (4.5%) were positive for Shigella species. The most common species of Shigella were S. flexneri (48%) and S. sonnei (45%); other results were S. dysenteriae (5%) and S. boydii (2%). The rate of Sensitivity to ceftriaxone (95%), ceftizoxime (94%), and nalidixic acid (84%) were among our isolates. Resistance to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Shigella; Antimicrobial susceptibility; Dysentery.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000200007
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Relationship among Shigella spp. and enteroinvasive Escherichia coli (EIEC) and their differentiation BJM
Ud-Din,Abu; Wahid,Syeda.
Shigellosis produces inflammatory reactions and ulceration on the intestinal epithelium followed by bloody or mucoid diarrhea. It is caused by enteroinvasive E. coli (EIEC) as well as any species of the genus Shigella, namely, S. dysenteriae, S. flexneri, S. boydii, and S. sonnei. This current species designation of Shigella does not specify genetic similarity. Shigella spp. could be easily differentiated from E. coli, but difficulties observed for the EIEC-Shigella differentiation as both show similar biochemical traits and can cause dysentery using the same mode of invasion. Sequencing of multiple housekeeping genes indicates that Shigella has derived on several different occasions via acquisition of the transferable forms of ancestral virulence plasmids...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diarrhea; E. coli; Shigella; Real-time PCR.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400002
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The importance of integrons for development and propagation of resistance in Shigella: the case of Latin America BJM
Barrantes,Kenia; Achí,Rosario.
Abstract In Latin America, the disease burden of shigellosis is found to coexist with the rapid and rampant spread of resistance to commonly used antibiotics. The molecular basis of antibiotic resistance lies within genetic elements such as plasmids, transposons, integrons, genomic islands, etc., which are found in the bacterial genome. Integrons are known to acquire, exchange, and express genes within gene cassettes and it is hypothesized that they play a significant role in the transmission of multidrug resistance genes in several Gram-negative bacteria including Shigella. A few studies have described antibiotic resistance genes and integrons among multidrug resistant Shigella isolates found in Latin America. For example, in Brazil, Bolivia, Chile, Costa...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Shigella; Antibiotic resistant; Integron; Gene cassettes; Latin America.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400800
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The normal chain length distribution of the O antigen is required for the interaction of Shigella flexneri 2a with polarized Caco-2 cells Biol. Res.
Hoare,Anilei; Bravo,Denisse; Martinic,Mara; Valvano,Miguel A; Contreras,Inés; Álvarez,Sergio A.
Shigella flexneri causes bacillary dysentery in humans. Essential to the establishment of the disease is the invasion of the colonic epithelial cells. Here we investigated the role of the lipopolysaccharide (LPS) O antigen in the ability of S. flexneri to adhere to and invade polarized Caco-2 cells. The S. flexneri 2a O antigen has two preferred chain lengths: a short O antigen (S-OAg) regulated by the WzzB protein and a very long O antigen (VL-OAg) regulated by Wzz pHS2. Mutants with defined deletions of the genes required for O-antigen assembly and polymerization were constructed and assayed for their abilities to adhere to and enter cultured epithelial cells. The results show that both VL- and S-OAg are required for invasion through the basolateral cell...
Tipo: Journal article Palavras-chave: Shigella; Epithelial cells; O antigen.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100003
Registros recuperados: 10
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