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A multicomponent system based on a blend of agroindustrial wastes for the simultaneous production of industrially applicable enzymes by solid-state fermentation Ciênc. Tecnol. Aliment.
OHARA,Andre; SANTOS,Jessika Gonçalves dos; ANGELOTTI,Joelise Alencar Figueira; BARBOSA,Paula de Paula Menezes; DIAS,Fernanda Furlan Gonçalves; BAGAGLI,Marcela Pavan; SATO,Hélia Harumi; CASTRO,Ruann Janser Soares de.
Abstract This study reports the use of statistical mixture design as a tool for the simultaneous production of lipase, CMCase, α-amylase, and β-glucosidase by Aspergillus niger under solid-state fermentation. Wheat bran, soybean meal, cottonseed meal, and orange peel were used as substrates, either individually or combined in different formulations, to study their synergistic or antagonistic effects on production of the enzymes. The highest lipase (323 U g-1) and CMCase (10 U g-1) activities were detected after 48 h, while the maximum activities of α-amylase (18 U g-1) and β-glucosidase (15 U g-1) occurred at 72 and 96 h, respectively. Considering the substrate formulation, the ternary mixture of wheat bran (1/3), soybean meal (1/3), and cottonseed meal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Industrial enzymes; Solid-state fermentation; Aspergillus niger; Agroindustrial wastes; Mixture design.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612018000500131
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Alpha amylase from a fungal culture grown on oil cakes and its properties BABT
Ramachandran,Sumitra; Patel,Anil K.; Nampoothiri,Kesavan Madhavan; Chandran,Sandhya; Szakacs,George; Soccol,Carlos Ricardo; Pandey,Ashok.
Solid-state fermentation was carried out for the production of alpha-amylase using Aspergillus oryzae. Different oil cakes such as coconut oil cake (COC) sesame oil cake (SOC), groundnut oil cake (GOC), palm kernel cake (PKC) and olive oil cake (OOC) were screened to be used as substrate for the enzyme production and also compared with wheat bran (WB). GOC was found to be the best producer of the enzyme among these. Combination of WB and GOC (1:1) resulted higher enzyme titres than the individual substrates. Maximum amount of enzyme (9196 U/gds) was obtained when SSF was carried out using WB + GOC, having initial moisture of 64% and supplemented with lactose and ammonium nitrate (1% each) at 30ºC for 72h using 2 mL spore suspension (6x10(7)spores/ml)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alpha amylase; Oil cakes; Solid-state fermentation; Partial purification; Aspergillus oryzae.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200019
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Aplicação da metodologia de superfície de resposta no estudo da produção e extração da poligalacturonase. Repositório Alice
SANTOS, S. F. de M.; MACEDO, G. R. de; SILVA, F. L. H. da; SOUZA, R. L. A. de; PINTO, G. A. S..
2008
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Solid-state fermentation; Cashew apple; Aspergillus niger.
Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/handle/doc/630853
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APPLICATION OF SOLID-STATE FERMENTATION FOR CELLULASE ENZYME PRODUCTION USING TRICHODERMA VIRIDE AgEcon
Mojsov, Kiro.
The Solid-state fermentation (SSF) is alternative to submerged fermentation for production antibiotics, single cell protein, enzymes, organic acids, biofuel, etc. However, the advantages of SSF in various processes are found to be greater than in submerged fermentation. This technique not only decreases the cost of the process but also makes product cheaper for consumers. The paper describes experimental application of SSF on wheat straw for production of mycelia protein and cellulase enzymes by Trichoderma viride. This actual waste from agriculture industry was used as a nourishing base by Trichoderma viride in SSF for cellulase enzyme production. Growth and enzymes production by Trichoderma viride were evaluated on wheat straw and alkali treatment...
Tipo: Journal Article Palavras-chave: Solid-state fermentation; Trichoderma viride; Cellulase enzyme; Wheat straw agro-waste; Single cell protein.; Research and Development/Tech Change/Emerging Technologies; L65.
Ano: 2010 URL: http://purl.umn.edu/92392
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Assessment of the effects of intermittent mixing on solid-state fermentation for biomass-degrading enzymes production by different fungal strains. Repositório Alice
CASSARO, E. G.; FONSECA, R. F.; FARINAS, C. S..
bitstream/item/180868/1/Assessment-of-the-effects-of-intermittent-mixing-on-solid-state.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Solid-state fermentation; Aspergillus Niger; Aspergillus Oryzae; Trichoderma reesei; Agitation; Cellulases; Sugarcane bagasse; Xylanases.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1035699
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Biochemical characterisation of a glucoamylase from Aspergillus niger produced by solid-state fermentation BABT
Slivinski,Christiane Trevisan; Machado,Alex Vinicius Lopes; Iulek,Jorge; Ayub,Ricardo Antônio; Almeida,Mareci Mendes de.
In this work, glucoamylase was produced by Aspergillus niger in solid-state fermentation. The enzyme was partially purified by ammonium sulphate precipitation and ion exchange and gel filtration chromatographies. Its molecular mass was estimated as 118.17 kDa by electrophoresis. The partially purified enzyme had an optimum pH range of 4.5-5.0 and an optimum temperature of 60 °C, with average activity 152.85 U mL-1. Thermal and pH stability assays with the crude extract showed that more than 60 % of the activity remained at pH 4.6 and 60 °C, even after an exposition to these conditions longer than 24 h. Yet, after purification, the enzyme was stable at these for at least 4 h, which indicated that its purification for use in starch saccharification was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucoamylase; Aspergillus niger; Solid-state fermentation; Biochemical characterization; Purification.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300018
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Bioconversion of agro-industrial wastes for the production of fibrinolytic enzyme from Bacillus halodurans IND18: Purification and biochemical characterization Electron. J. Biotechnol.
Vijayaraghavan,Ponnuswamy; Prakash Vincenta,Samuel Gnana; Valan Arasu,Mariadhas; Al-Dhabi,Naif Abdullah.
Background: Agro-wastes were used for the production of fibrinolytic enzyme in solid-state fermentation. The process parameters were optimized to enhance the production of fibrinolytic enzyme from Bacillus halodurans IND18 by statistical approach. The fibrinolytic enzyme was purified, and the properties were studied. Results: A two-level full factorial design was used to screen the significant factors. The factors such as moisture, pH, and peptone were significantly affected enzyme production and these three factors were selected for further optimization using central composite design. The optimum medium for fibrinolytic enzyme production was wheat bran medium containing 1% peptone and 80% moisture with pH 8.32. Under these optimized conditions, the...
Tipo: Journal article Palavras-chave: Agro-residues; Solid-state fermentation; Statistical approach; Thrombolytic agent.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000200001
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Biodegradation of remazol brilliant blue R by ligninolytic enzymatic complex produced by Pleurotus ostreatus BJM
Machado,Kátia Maria Gomes; Matheus,Dácio Roberto.
Pleurotus ostreatus ("shimeji") is produced in Brazil on a commercial scale using various lignocellulosic residues. Efforts have been made to reuse the culture residue to obtain products of greater aggregate value such as enzymes or in processes of bioremediation. We evaluated the Remazol brilliant blue R (RBBR) degradation potential of extracts from solid substrate colonized by P. ostreatus and extracts from residue of the "shimeji" mushroom yield. Colonized substrates and residue were provided by Toyobo do Brasil Ltda. Extraction was performed with sodium acetate buffer (50 mM, pH 4.6). RBBR decolorization was monitored at 592 nm and peroxidase and laccase activities were measured by monitoring the oxidation of ABTS. Horseradish peroxidase was used as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Peroxidases; Laccase; Solid-state fermentation; "shimeji" culture residue.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400013
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Characterization of a multi-tolerant tannin acyl hydrolase II from Aspergillus carbonarius produced under solid-state fermentation Electron. J. Biotechnol.
Valera,Larissa Serrani; Jorge,João Atílio; Guimarães,Luis Henrique Souza.
Background Tannases are enzymes with biotechnological potential produced mainly by microorganisms as filamentous fungi. In this context, the production and characterization of a multi-tolerant tannase from Aspergillus carbonarius is described. Results The filamentous fungus A. carbonarius produced high levels of tannase when cultivated under solid-state fermentation using green tea leaves as substrate/carbon source and tap water at a 1:1 ratio as the moisture agent for 72 h at 30°C. Two tannase activity peaks were obtained during the purification step using DEAE-Cellulose. The second peak (peak II) was purified 11-fold with 14% recovery from a Sepharose CL-6B chromatographic column. The tannase from peak II (tannase II) was characterized as a heterodimeric...
Tipo: Journal article Palavras-chave: Aspergillus; Microbial enzymes; Solid-state fermentation; Tannase; Tannic acid.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600013
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Characterization of biotechnologically relevant extracellular lipase produced by Aspergillus terreus NCFT 4269.10 BJM
Sethi,Bijay Kumar; Nanda,Prativa Kumari; Sahoo,Santilata.
Abstract Enzyme production by Aspergillus terreus NCFT 4269.10 was studied under liquid static surface and solid-state fermentation using mustard oil cake as a substrate. The maximum lipase biosynthesis was observed after incubation at 30 °C for 96 h. Among the domestic oils tested, the maximum lipase biosynthesis was achieved using palm oil. The crude lipase was purified 2.56-fold to electrophoretic homogeneity, with a yield of 8.44%, and the protein had a molecular weight of 46.3 kDa as determined by SDS-PAGE. Enzyme characterization confirmed that the purified lipase was most active at pH 6.0, temperature of 50 °C, and substrate concentration of 1.5%. The enzyme was thermostable at 60 °C for 1 h, and the optimum enzyme–substrate reaction time was 30...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus terreus; Lipase; Liquid static surface fermentation; Solid-state fermentation.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100143
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Citric acid production from orange peel wastes by solid-state fermentation BJM
Torrado,Ana María; Cortés,Sandra; Salgado,José Manuel; Max,Belén; Rodríguez,Noelia; Bibbins,Belinda P; Converti,Attilio; Domínguez,José Manuel.
Valencia orange (Citrus sinensis) peel was employed in this work as raw material for the production of citric acid (CA) by solid-state fermentation (SSF) of Aspergillus niger CECT-2090 (ATCC 9142, NRRL 599) in Erlenmeyer flasks. To investigate the effects of the main operating variables, the inoculum concentration was varied in the range 0.5·10³ to 0.7·10(8) spores/g dry orange peel, the bed loading from 1.0 to 4.8 g of dry orange peel (corresponding to 35-80 % of the total volume), and the moisture content between 50 and 100 % of the maximum water retention capacity (MWRC) of the material. Moreover, additional experiments were done adding methanol or water in different proportions and ways. The optimal conditions for CA production revealed to be an...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Orange peel; Citric acid; Aspergillus niger; Solid-state fermentation.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100049
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Comparison of Aspergillus niger spore production on Potato Dextrose Agar (PDA) and crushed corncob medium. Repositório Alice
FARIAS, V. L. de; MONTEIRO, K. X.; RODRIGUES, S.; FERNANDES, F. A. N.; PINTO, G. A. S..
2010
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Aspergillus niger; Corncob; Low-cost medium; Solid-state fermentation.
Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/handle/doc/877901
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Co-Production of Nattokinase and Poly (γ-Glutamic Acid) Under Solid-State Fermentation Using Soybean and Rice Husk BABT
Nie,Guangjun; Zhu,Zhubin; Liu,Fang; Nie,Zhijie; Ye,Yuchao; Yue,Wenjin.
ABSTRACTThe aim of this work was to study the co-production of nattokinase and poly (γ-glutamic acid) by Bacillus subtilis natto with soybean and rice husk under solid-state fermentation (SSF). The results showed that the size of soybean particle and rice husk significantly improved the co-production of nattokinase and poly (γ-glutamic acid), yielding 2503.4 IU/gs and 320 mg/gs, respectively in the improved culture medium composed of 16.7% soybean flour and 13.3% rice husk with 70% water content. The yields increased by approximate 7- and 2-fold factor relative to their original ones. Thus, the co-production of nattokinase and poly (γ-glutamic acid) under SSF could be considered as an efficient method to exploit agro-residues for economical production of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Co-production; Nattokinase; Poly (γ-glutamic acid); Rice husk; Solid-state fermentation.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500718
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Enhanced antioxidant activity of okara through solid state fermentation of GRAS Fungi Ciênc. Tecnol. Aliment.
SITANGGANG,Azis Boing; SINAGA,Wenny Silvia Loren; WIE,Felicia; FERNANDO,Franz; KRUSONG,Warawut.
Abstract The enhancement of antioxidant activity of fermented okara was investigated using fungal solid-state fermentation. The initial fungal concentration and water activity were found to influence the enhanced antioxidant capacity of fermented okara. Extracellular enzymes, especially proteases and carbohydrases might facilitate the hydrolysis of okara protein that yielded antioxidative amino acids and peptides, as well as the release of isoflavones-derived components. Fermentation of okara with R. oligosporus with 1.04×10-3 gDCW/gokara as initial concentration, water activity of 0.931 was found to yield the highest antioxidant strength. Whereas for A. Oryzae, 1.6×10-3 gDCW/gokara was optimum as the initial concentration with water activity of 0.913....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant; A. oryzae; Okara; R. oligosporus; Solid-state fermentation.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019005026105
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Improvement of xylanase production by Cochliobolus sativus in solid state fermentation BJM
Bakri,Yasser; Jawhar,Mohammed; Arabi,Mohammed Imad Eddin.
The xylanase production by Cochliobolus sativus strain Cs6 was improved under solid state fermentation (SSF). High xylanase activity (1079 U/g) was obtained when wheat straw was used after 8 days of incubation. Combinations of sodium nitrate with peptone or yeast extract resulted in an increased xylanase production (1543 and 1483 U/g, respectively). The Cs6 strain grown in SSF in a simple medium, proved to be a promising microorganism for xylanase production.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cochliobolus sativus; Xylanase; Solid-state fermentation.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300036
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Lipase production by solid-state fermentation in fixed-bed bioreactors BABT
Cavalcanti,Elisa d'Avila Costa; Gutarra,Melissa Limoeiro Estrada; Freire,Denise Maria Guimarães; Castilho,Leda dos Reis; Sant'Anna Júnior,Geraldo Lippel.
In the present work, packed bed bioreactors were employed with the aim of increasing productivity and scaling up of lipase production using Penicillium simplicissimum in solid-state fermentation. The influence of temperature and air flow rate on enzyme production was evaluated employing statistical experimental design, and an empirical model was adjusted to the experimental data. It was shown that higher lipase activities could be achieved at lower temperatures and higher air flow rates. The maximum lipase activity (26.4 U/g) was obtained at the temperature of 27°C and air flow rate of 0.8 L/min.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solid-state fermentation; Fixed-bed bioreactors; Lipase; Penicillium simplicissimum.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400010
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Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach BJM
Shabbiri,Khadija; Adnan,Ahmad; Jamil,Sania; Ahmad,Waqar; Noor,Bushra; Rafique,H.M..
Various cultivation parameters were optimized for the production of extra cellular protease by Brevibacterium linens DSM 20158 grown in solid state fermentation conditions using statistical approach. The cultivation variables were screened by the Plackett-Burman design and four significant variables (soybean meal, wheat bran, (NH4)2SO4 and inoculum size were further optimized via central composite design (CCD) using a response surface methodological approach. Using the optimal factors (soybean meal 12.0g, wheat bran 8.50g, (NH4)2SO4) 0.45g and inoculum size 3.50%), the rate of protease production was found to be twofold higher in the optimized medium as compared to the unoptimized reference medium.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brevibacterium linens; Protease; Solid-state fermentation; Plackett-Burman Design; Central Composite Design (CCD).
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300031
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Microbial rennet produced by Mucor miehei in solid-state and submerged fermentation BABT
Silveira,Guilherme Garcia da; Oliveira,Gustavo Monteiro de; Ribeiro,Eloizio Julio; Monti,Rubens; Contiero,Jonas.
The aim of this work was to study the effect of carbon and nitrogen sources in submerged fermentation, and the casein effect on solid-state fermentation on rennin production by Mucor miehei. Biomass peaks reached 6.7; 8.1 and 8 g/L and enzymatic production peaks of 1,066; 857 and 480 Soxhlet Units (S.U.) for glucose concentration of 18; 25 and 35 g/L respectively. Flasks with baffles showed biomass peaks of 6.7; 8.3 and 10 g/L and enzyme activity peaks of 648; 279 and 300 S.U. for the same glucose concentration. The values of 923 and 667 S.U. were obtained when corn steep liquor and Proflo (Cottonseed Nutrients from Traders ®) were used. In SSF system the enzymatic activities were 414, 264 and 167 S.U., when using HCl 0.2 N, 0.3 N and 0.4 N respectively....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mucor miehei; Rennin; Clotting activity; Casein; Solid-state fermentation; Submerged fermentation.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800009
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Novel process for the simultaneous extraction and degumming of banana fibers under solid-state cultivation BJM
Jacob,Nicemol; Prema,Parukuttyamma.
Various process parameters for the production of polygalacturonase by Streptomyces lydicus under solid-state fermentation were optimized. The optimum particle size of wheat bran for polygalacturonase production was in the range of 500-1000 µm. Initial moisture content of 70% was found to be the optimum for enzyme production. The most suitable inoculum size was 1.25 x 10(5) CFU/mL and the optimum incubation temperature was 30ºC. Addition of carbon sources resulted in 37% increase in enzyme yield (425 U/g), whereas no significant enhancement was obtained on nitrogen supplementation. Maximum enzyme yield was recorded at 72 h. When compared to the initial production medium (108.5 U/g), the enzyme yield was 3.9 fold after optimization. Solid-state fermentation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Banana fiber degumming; Solid-state fermentation; Polygalacturonase; Streptomyces lydicus; Wheat bran.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100025
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Partial characterization of an inulinase produced by Aspergillus japonicus URM5633 BABT
Silva,Anna Carolina da; Queiroz,Alana Emilia Soares de França; Porto,Tatiana Souza; Spier,Michele Rigon; Soccol,Carlos Ricardo; Porto,Ana Lúcia Figueiredo; Souza-Motta,Cristina Maria; Moreira,Keila Aparecida.
Enzymes obtained by fermentation processes offer a number of advantages and have been widely researched and used throughout the world. This study aimed to partially characterise an inulinase produced from palm and cassava peel. The enzyme was produced via the solid-state fermentation of Aspergillus japonicus URM5633. The optimal temperatures were 50ºC and 55ºC, and the optimal pH values were 5.2 and 3.4 for inulinase fermentatively produced from palm and cassava peel, respectively. The thermostability measurements for inulinase produced in palm showed that the relative activity remained below 100% until 30 minutes of stability for all temperatures, but reached 106.8% at a temperature of 50ºC after 60 minutes. Inulinase from the crude extract of cassava...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Inulinase; Characterisation; Solid-state fermentation; Aspergillus japonicus.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500005
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