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Advantages and Applications of Cryopreservation in Fisheries Science BABT
Tsai,S.; Lin,C..
Cryopreservation is a long-term storage technique to preserve the biological material without deterioration for extended period of time at least several thousands of years. The ability to preserve and store both maternal and paternal gametes provides a reliable source of fish genetic material for scientific and aquaculture purposes as well as for conservation of biodiversity. Successful cryopreservation of fish sperm have been achieved for more than 200 fish species and many fish species have been adequated for the purpose of cryobanking. Cryopreservation of fish embryo is not viable, mainly because of the same limitations as in fish oocytes, i.e., high chilling sensitivity and low membrane permeability. However, cryopreservation of isolated embryonic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cryopreservation; Sperm; Embryo; Oocyte; Blastomere.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300014
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An Acute-phase Protein as a Regulator of Sperm Survival in the Bovine Oviduct: Alpha 1-acid-glycoprotein Impairs Neutrophil Phagocytosis of Sperm In Vitro OAK
LIU, Jinghui; A. MAREY, Mohamed; KOWSAR, Rasoul; HAMBRUCH, Nina; SHIMIZU, Takashi; HANEDA, Shingo; MATSUI, Motozumi; SASAKI, Motoki; HAYAKAWA, Hiroyuki; PFARRER, Christiane; MIYAMOTO, Akio; 羽田, 真悟; 松井, 基純; 佐々木, 基樹; 宮本, 明夫.
We have previously shown that polymorphonuclear neutrophils (PMNs) are present in bovine oviduct fluid under physiological conditions, and that the oviduct provides a microenvironment that protects sperm from phagocytosis by PMNs. Alpha 1-acid glycoprotein (AGP) is a major acute-phase protein produced mainly in the liver that has immunomodulatory functions. AGP mRNA is expressed in extrahepatic organs, such as the lung, kidney, spleen, lymph node, uterus, and ovary. Therefore, in this study, we investigated, 1) the local production of AGP in the bovine oviduct, 2) the effect of AGP on the phagocytic activity of PMNs for sperm and superoxide production and 3) the impact of AGP desialylation on the PMN phagocytosis of sperm. The AGP gene was expressed in...
Palavras-chave: Alpha 1-acid glycoprotein (AGP); Bovine; Oviduct; Phagocytosis; Sperm.
Ano: 2014 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3946
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Antioxidant effect on viability of boar semen cooled to 15°C Arq. Bras. Med. Vet. Zootec.
Martins,V.E.D.; Pinto,S.C.C.; Chaves,R.M.; Barros Filho,A.K.D.; Laskoski,L.M.; Souza,F.A..
ABSTRACT This study aimed to evaluate the addition of Vitamin C, reduced Glutathione and trolox on sperm characteristics of pork refrigerated semen. Six pigs were collected through the technique of gloved hand (10 ejaculates/animals). The semen was diluted in MR-A®. After the previous evaluations, the treatments were added: Control group: diluent only; Vitamin C Group: 200μM/mL Vitamin C; Trolox Group: 200μM/mL Trolox; Glutathione group: 2.5mM/ml Reduced glutathione. The semen was stored in thermal boxes and placed inside the refrigerator at 15oC and evaluated at D0, 12, 48, 72 hours. After 30 hours of incubation, each treatment was divided into two equal fractions and the same concentration of antioxidants was added in one of the parts. The results show...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glutathione; Sperm; Trolox; Vitamin C.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000100145
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Assessment of body and scrotal thermoregulation and semen quality of hair sheep rams throughout the year in a tropical environment. Repositório Alice
KAHWAGE, P. R.; ESTEVES, S. N.; JACINTO, M. A. C.; BARIONI JUNIOR, W.; MACHADO, R.; ROMANELLO, N.; PASSERI, L. F.; MENDONÇA, K. L. de; GARCIA, A. R..
The present study was carried out to assess the capacity of Morada Nova (MON) and Santa Inês (SIN) hair sheep breeds to maintain homeothermy and its relation with semen quality in a tropical climate. Sixteen rams (MON=7 and SIN=9) were assessed for 12 months. During summer and spring, the Temperature-Humidity Index values surpassed the level indicative of thermal stress, and sweating, respiratory and heart rates increased (P < .05). Those responses allowed animals to dissipate heat, maintain rectal temperature in the physiological range and efficiently avoid thermal stress. Scrotal temperatures rose during summer and spring, but, unlike what occurs in wool breeds of sheep, the thermal gradient between poles of testis increased, which shows efficient...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Tropical climate; Thermal comfort; Adaptability; Sperm; Clima Tropical; Esperma; Ovis Aries; Sheep.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1095409
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Binding patterns of seminal plasma plasma proteins on bovine epididymal and ejaculated sperm membrane Arq. Bras. Med. Vet. Zootec.
Souza,C.E.A.; Moura,A.A.; Lima-Souza,A.C.; Killian,G.J..
The present study was designed to investigate the topographical distribution of seminal plasma (SP) proteins on epididymal and ejaculated bovine sperm. Using immunocytochemistry and confocal microscopy the binding patterns of bovine SP proteins BSP-A3, albumin, transferrin, prostaglandin D-synthase (PGDS) and nucleobindin in ejaculated and cauda epididymal sperm from adult bulls were evaluated. Experiments were performed using sperm from 5 males. Data showed a positive signal, only detected for anti-PGDS, in the acrosomal cap of epididymal and ejaculated sperm. In ejaculated sperm, a very weak signal for nucleobindin 2 in the midpiece and equatorial regions was detected, using the anti-rat nucleobindin. BSP-A3 was detected on all sperm regions studied,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sperm; Seminal plasma proteins; Immunocytochemistry.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000300001
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Biological characteristics of sperm in European flat oyster ( Ostrea edulis ) ArchiMer
Suquet, Marc; Pouvreau, Stephane; Queau, Isabelle; Boulais, Myrina; Le Grand, Jacqueline; Ratiskol, Dominique; Cosson, Jacky.
n brooding mollusc species that incubate larvae before their release in seawater, the biology of gametes remains poorly explored. The present study describes some biological characteristics of sperm of the European flat oyster, a native species that has been over-exploited in the past and is nowadays classified as an endangered species in Europe. Flat oysters were collected by divers in the Rade de Brest (Finistère, France), during its natural reproduction period. Gonadal pH is acidic (6.31 ± 0.10). Spermatozoa are clustered in spermatozeugmata, an acellular structure in which the sperm heads are embedded. After their transfer in seawater, spermatozeugmata have a mean diameter of 64 ± 3 μm and they release free spermatozoa for a mean duration of...
Tipo: Text Palavras-chave: Oyster; Ostrea edulis; Brooding species; Sperm; Spermatozeugmata; Endangered species.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00454/56539/58245.pdf
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Caracteristiques ultrastructurales du spermatozoide de la coquille Saint-Jacques, Pecten maximus (Mollusque, Bivalve) ArchiMer
Dorange, G; Lepennec, M.
The spermatozoon of Pecten maximus is of the primitive type usually associated with species having external fertilization or emitting the sperm in the water. The size of the nucleus is reduced. The middle piece is similar to that reported for several Bivalves. It contains glycogen. But the spermatozoon of P. maximus exhibits its own ultrastructural characteristics: the short acrosome differs in size, shape, position and ultrastructure or by a combination of these from that of many bivalves. No axial rods was observed in contrast to several species of Mytilidae, Veneridae and Ostreidae. These originalities could go to the genus level.
Tipo: Text Palavras-chave: Pecten maximus; Bivalvia; Ultrastructure; Sperm.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/acte-1725.pdf
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Changes in Atlantic cod (Gadus morhua L.) sperm quality during the spawning season ArchiMer
Rouxel, Catherine; Suquet, Marc; Cosson, J; Severe, Armelle; Quemener, Loic; Fauvel, Christian.
The biology of cod reproduction is well described in the scientific literature. However, sperm biology and spermatozoa management are poorly studied in this species. Because of its recent farming expansion, a better knowledge of cod gametes is becoming especially useful. This work aimed at establishing tools to study sperm biology in cod, and also investigated the existence of changes in cod sperm quality during the spawning period. We showed that sperm concentration could be assessed using spectrophotometry at 260 nm. Sperm motility significantly decreased after a 168-h storage at 4 degrees C. A 1:9 dilution of sperm in a non-activating medium (1/3 seawater and 2/3 freshwater, osmotic pressure: 360 mOsm kg(-1)) improved sperm storage. Sperm concentration,...
Tipo: Text Palavras-chave: Spermatozoa; Quality; Ageing; Sperm; Cod; Gadus morhua.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3829.pdf
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Changes in motility, ATP content, morphology and fertilisation capacity during the movement phase of tetraploid Pacific oyster (Crassostrea gigas) sperm ArchiMer
Suquet, Marc; Labbe, C.; Brizard, Raphael; Donval, Anne; Le Coz, Jean-rene; Quere, Claudie; Haffray, P..
Changes in sperm features during the movement phase are especially interesting to study in external fertilization species whose sperm duration movement is long because this implies a significant adaptation of moving cells to the external medium. This study describes the changes in tetraploid Pacific oyster sperm characteristics in relation to time post activation. Sperm individually collected on three tetraploid males were activated in seawater. Their features were analysed over a 24 h period and compared to a sperm pool collected on three diploid males as a reference. The percentage of motile spermatozoa, the intracellular ATP content, and the fine structure of spermatozoa were studied in relation to time post activation. Furthermore, the fertilisation...
Tipo: Text Palavras-chave: Crassostrea gigas; Sperm; Tetraploid; Motility; ATP; Ultrastructure.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00006/11756/8639.pdf
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Characteristics of the Male Reproductive System and Spermatozoa of Leptophlebiidae (Ephemeroptera) Neotropical Entomology
Brito,P; Salles,FF; Dolder,H.
This study describes morphological changes in the male reproductive system of Miroculis amazonicus (Savage &amp; Peters) from mature nymphs to subimago stages. The sperm ultrastructure of Massartela brieni (Lestage), Farrodes carioca (Domínguez et al) and Miroculis mourei (Savage &amp; Peters), as well as aspects of cell fragments observed in these species' subimagos deferent ducts were described. Sperm from the three species studied are aflagellated and immotile, while those from F. carioca and Ma. brieni are approximately spherical with a homogenous nucleus and acrosome. Sperm of F. carioca present two or three mitochondria located between the nucleus and the acrosome. In Ma. brieni, only one lateral mitochondria was found. Sperm from Mi. mourei...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Morphology; Sperm; Aflagellate; Sexual maturation.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2011000100015
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Characterization of sperm motility in sea bass: the effect of heavy metals and physicochemical variables on sperm motility ArchiMer
Abascal, F; Cosson, J; Fauvel, Christian.
Computer assisted sperm analysis (CASA) was used to characterize the motility of sea bass Dicentrarchus labrax spermatozoa and to study the effect of several physicochemical variables and heavy metals on sperm swimming performance. Duration of sperm motility in sea bass was very short (< 50 s). During the first 20 s all the motility variables measured remained approximately constant, the velocity and linearity of the movement being maximum during this period, while both variables decreased sharply later. While slight variations in pH did not significantly modify sperm swimming performance, changes in osmolality affected all the measured motility variables. Two of the heavy metals tested, Cu2+ and Pb2+, did not affect sperm motility when the activating...
Tipo: Text Palavras-chave: Sperm; Heavy metals; Dicentrarchus labrax; CASA.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-4765.pdf
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Comparative description and discussion of spermiogenesis and spermatozoal ultrastructure in some species of Heptapteridae and Pseudopimelodidae (Teleostei: Siluriformes) Neotropical Ichthyology
Quagio-Grassiotto,Irani; Spadella,Maria Angélica; Carvalho,Márcio de; Oliveira,Claudio.
The data obtained in the present study on spermiogenesis and spermatozoal ultrastructure of Pseudopimelodidae and Heptapteridae show that they share some characteristics, but greatly differ from each other. The main differences are the occurrence of type I spermiogenesis in Pseudopimelodidae and type III in Heptapteridae, the presence of nuclear fossa in Pseudopimelodidae and its absence in Heptapteridae, the presence of long midpiece in Pseudopimelodidae and short midpiece in Heptapteridae, the presence of cytoplasmic canal in Pseudopimelodidae and its absence in Heptapteridae, the presence of many large vesicles in the midpiece of Pseudopimelodidae and the presence of very long vesicles placed in the peripheral distal region in Heptapteridae, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Catfish; Spermatid; Sperm; Structure; Phylogeny.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252005000300008
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Cryopreservation of mutton snapper (Lutjanus analis) sperm Anais da ABC (AABC)
SANCHES,EDUARDO G.; OLIVEIRA,IDILI R.; SERRALHEIRO,PEDRO C. DA SILVA; CERQUEIRA,VINICIUS R..
This study aimed to develop a protocol of semen cryopreservation of the mutton snapper Lutjanus analis. The interaction between three extenders ( pH 6.1; 7.8 and 8.2) , two concentrations of dimethyl sulfoxide ( DMSO, 5 and 10%) and three cooling rates ( -90; -60 and -30°C.min−1) on the sperm motility rate and motility time were analyzed by a factorial experiment. A sample of 30 fishes ( 1,261 ± 449 g) collected in the nature was kept in floating net cages. The semen was frozen by using cryogenic straws, in nitrogen vapour and transferred, later, to liquid nitrogen. Fertilization test was accomplished to evaluate the viability of the cryopreserved sperm. The highest sperm motility rate and motility time ( P < 0.05) was achieved by combining extender C (...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cryoprotector; Extender; Lutjanus analis; Reproduction; Sperm.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000301083
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Cycles sexuels de quelques poissons plats des cotes bretonnes. ArchiMer
Lahaye, Jacqueline.
Representative species of the families Pleuronectidae, Bothidae and Soleidae were studied throughout the year. Changes are described which were seen in the ovaries, and photographs of cross sections of the tissue are shown. The progress of egg release was noted, and in some cases correlated studies were made of gonadal changes in male. The period of egg release was prolonged over 1 one half -2 months for plaice and turbot, and for 6 months for sole. Individual fish showed these extended periods of laying, and the actual months of the year differed for each species. The massive mobilisation of reserves which necessarily accompanied the release of large numbers of eggs was reflected by turbot, in sperm production. In addition, the increase in gonadosomatic...
Tipo: Text Palavras-chave: Gonadosomatic activity; Sperm; Species; Brittany coasts; Flat fishes; Sexual cycles.
Ano: 1972 URL: http://archimer.ifremer.fr/doc/1972/publication-2064.pdf
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Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization Biol. Res.
Cohen,Débora J; Maldera,Julieta A; Weigel Muñoz,Mariana; Ernesto,Juan I; Vasen,Gustavo; Cuasnicu,Patricia S.
Epididymal protein CRISPI is a member of the CRISP (Cysteine-RIch Secretory proteins) family and is involved in sperm-egg fusion through its interaction with complementary sites on the egg surface. Results from our laboratory have shown that this binding ability resides in a 12-amino-acid region corresponding to a highly conserved motif of the CRISP family, named Signature 2 (S2). In addition to this, our results revealed that CRISP1 could also be involved in the previous step of sperm binding to the zona pellucida, identifying a novel role for this protein in fertilization. As another approach to elucidate the participation of CRISP1 in fertilization, a mouse line containing a targeted disruption of CRISP1 was generated. Although CRISP1-deficient mice...
Tipo: Journal article Palavras-chave: Sperm; Gamete fusion; CRISP; Egg; Fertilization.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200004
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Development of single tubing-type canister for cryo-storage of bull semen and their effect on sperm motility and viability BABT
Ismail,Mohd Iswadi; Osman,Khairul; Ibrahim,Siti Fatimah; Jaafar,Farah Hanan Fatihah; Ghani,Nur Azianie Abd; Zainalabidin,Fazly Ann; Othman,Abas Mazni.
The objective of this study was to evaluate the potential of using single tubing-type canister on sperm quality. Semen was collected from the Bali cattle bull by electroejaculation technique and was cryopreserved in liquid nitrogen using slow freezing cryopreservation method. Two type of canister volume was used in this study; commercial canister (342.25π x 278 mm²) and single tubing-type canister (4π x 90 mm²). Makler counting chamber and computer assisted sperm analyzer (CASA) were used to evaluate the sperm motility and viability of post-thaw sperm. Results showed that the bull sperm motility and viability at the bottom of tubing-type canister was statistically higher and significant as compared to the commercial canister (p<0.05). Significant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cryopreservation; Sperm; Canister; Motility; Viability.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200006
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Effect of Diet Contains Sesame Seed on Epididymal Histology of Adult Rat International Journal of Morphology
Mahabadi J,Amini; Khodayari,M; Bafrani H,Hassani; Nikzad,H; Taherian,A.
Sesamin is a major lignan constituent of sesame seed and considered as a key factor in a number of beneficial effects on human health. Sesame leaves intake improve and increase epididymal spermatocytes reserve in adult male sprague dawley rat. The present study was aimed to investigate the effects of contain sesame seed on epididymis histologhical structure. Thirty adult male rats were divided into two groups of 15 rats each. The regimen group received diet containing 30% sesame seed, while the control group received diet along 12 weeks. The right epididymis were removed and minced into several pieces on a specimen bottle containing normal saline for some few minutes to allow the sperms to become motile and swim out and sperm parameters were analyzed. Left...
Tipo: Journal article Palavras-chave: Diet; Epididymis; Sesame seed; Sperm; Hormone.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100042
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Effect of Sesame-Supplemented Diet on Prostate and Seminal Vesicle Histology of Adult Rat International Journal of Morphology
Mahabadi,Javad Amini; Bafrani,Hassan Hassani; Nikzad,Hossein.
Infertility is a great concern among the people of reproductive age. The use of natural products obtained from traditional herbs is appealing. Studies show that antioxidants are important in improving male infertility. Thirty adult male rats were randomly divided into two regimen and control groups. The regimen group received diet containing 30 % sesame seed, while the control group received standard diet for 12 weeks. Histology of prostate and seminal vesicle were evaluated and serum levels of FSH, LH and testosterone concentrations were assessed as well. The results showed that, the diameter of peripheral epithelium and the volume density of the prostate epithelium increased but the volume density of the prostate stroma was decreased significantly in the...
Tipo: Journal article Palavras-chave: Prostate; Seminal vesicle; Sesame seed; Sperm; Hormone.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000200030
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Endothelin-1 downregulates sperm phagocytosis by neutrophils in vitro: a physiological implication in bovine oviduct immunity OAK
MAREY, Mohamed Ali; YOUSEF, Mohamed Samy; LIU, Jinghui; MORITA, Kazuhiro; SASAKI, Motoki; HAYAKAWA, Hiroyuki; SHIMIZU, Takashi; ELSHAHAWY, Ibrahim I.; MIYAMOTO, Akio; 佐々木, 基樹; 清水, 隆; 宮本, 明夫.
The oviduct is an active contractile tube that provides the proper environment for sperm transport, capacitation and survival. Oviductal contractions are regulated by autocrine/paracrine secretion of several factors, such as prostaglandins (PGs) and endothelin-1 (EDN-1). We have previously shown that during the preovulatory stage, sperm are exposed to polymorphonuclear neutrophils (PMNs) in the bovine oviduct, and the bovine oviduct epithelial cells (BOECs) secrete molecules including PGE2 that suppress sperm phagocytosis by PMNs in vitro. In this study, we investigated the possible effects of EDN-1 on the phagocytic activity of PMNs toward sperm. The local concentrations of EDN-1 in oviduct fluid and BOEC culture medium ranged from 10–10 to 10–11 M as...
Palavras-chave: Endothelin-1; Oviduct; Phagocytosis; Polymorphonuclear neutrophils (PMN); Sperm.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4310
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Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks (Capra hircus). Repositório Alice
PINTO, T. M. F.; MOREIRA, R. F.; MATOS, M. N. C.; SOARES, V. V. M.; AGUIAR, M. V. de A.; ARAGÃO, P. de T. T. D. de; ALVES FILHO, J. G.; MORENO, F. B. M. B.; MONTEIRO-MOREIRA, A. C. de O.; COSTA, C. R. R.; LIMA FILHO, J. L. de; ELOY, A. M. X.; CUNHA, R. M. S. da.
Abstract: The Saanen goat breed has been widely explored in breeding programmes; however, there are few reports about the breed?s genetic and molecular composition. Thus, this study aimed to characterize the proteomic profile of spermatozoa from Saanen breeding goats. Five breeding animals with proven fertility were selected, the spermatozoa were collected, and the protein was extracted. Subsequently, the proteins were separated and analysed by two-dimensional electrophoresis and mass spectrometry; the proteins were then identified with the SwissProt database. A total of 31 proteins involved in reproduction were identified, including binding proteins on spermatozoa for fusion with the egg, acrosomal membrane proteins, metabolic enzymes, heat shock...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Sperm; Profiles; Proteomic profile; Brasil; Brazilian Northeast; Caprino; Goats; Saanen; Proteomics; Spermatogenesis; Semiarid zones.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1120171
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