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Registros recuperados: 37
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An Acute-phase Protein as a Regulator of Sperm Survival in the Bovine Oviduct: Alpha 1-acid-glycoprotein Impairs Neutrophil Phagocytosis of Sperm In Vitro OAK
LIU, Jinghui; A. MAREY, Mohamed; KOWSAR, Rasoul; HAMBRUCH, Nina; SHIMIZU, Takashi; HANEDA, Shingo; MATSUI, Motozumi; SASAKI, Motoki; HAYAKAWA, Hiroyuki; PFARRER, Christiane; MIYAMOTO, Akio; 羽田, 真悟; 松井, 基純; 佐々木, 基樹; 宮本, 明夫.
We have previously shown that polymorphonuclear neutrophils (PMNs) are present in bovine oviduct fluid under physiological conditions, and that the oviduct provides a microenvironment that protects sperm from phagocytosis by PMNs. Alpha 1-acid glycoprotein (AGP) is a major acute-phase protein produced mainly in the liver that has immunomodulatory functions. AGP mRNA is expressed in extrahepatic organs, such as the lung, kidney, spleen, lymph node, uterus, and ovary. Therefore, in this study, we investigated, 1) the local production of AGP in the bovine oviduct, 2) the effect of AGP on the phagocytic activity of PMNs for sperm and superoxide production and 3) the impact of AGP desialylation on the PMN phagocytosis of sperm. The AGP gene was expressed in...
Palavras-chave: Alpha 1-acid glycoprotein (AGP); Bovine; Oviduct; Phagocytosis; Sperm.
Ano: 2014 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3946
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Assessment of body and scrotal thermoregulation and semen quality of hair sheep rams throughout the year in a tropical environment. Repositório Alice
KAHWAGE, P. R.; ESTEVES, S. N.; JACINTO, M. A. C.; BARIONI JUNIOR, W.; MACHADO, R.; ROMANELLO, N.; PASSERI, L. F.; MENDONÇA, K. L. de; GARCIA, A. R..
The present study was carried out to assess the capacity of Morada Nova (MON) and Santa Inês (SIN) hair sheep breeds to maintain homeothermy and its relation with semen quality in a tropical climate. Sixteen rams (MON=7 and SIN=9) were assessed for 12 months. During summer and spring, the Temperature-Humidity Index values surpassed the level indicative of thermal stress, and sweating, respiratory and heart rates increased (P < .05). Those responses allowed animals to dissipate heat, maintain rectal temperature in the physiological range and efficiently avoid thermal stress. Scrotal temperatures rose during summer and spring, but, unlike what occurs in wool breeds of sheep, the thermal gradient between poles of testis increased, which shows efficient...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Tropical climate; Thermal comfort; Adaptability; Sperm; Clima Tropical; Esperma; Ovis Aries; Sheep.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1095409
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Biological characteristics of sperm in European flat oyster ( Ostrea edulis ) ArchiMer
Suquet, Marc; Pouvreau, Stephane; Queau, Isabelle; Boulais, Myrina; Le Grand, Jacqueline; Ratiskol, Dominique; Cosson, Jacky.
n brooding mollusc species that incubate larvae before their release in seawater, the biology of gametes remains poorly explored. The present study describes some biological characteristics of sperm of the European flat oyster, a native species that has been over-exploited in the past and is nowadays classified as an endangered species in Europe. Flat oysters were collected by divers in the Rade de Brest (Finistère, France), during its natural reproduction period. Gonadal pH is acidic (6.31 ± 0.10). Spermatozoa are clustered in spermatozeugmata, an acellular structure in which the sperm heads are embedded. After their transfer in seawater, spermatozeugmata have a mean diameter of 64 ± 3 μm and they release free spermatozoa for a mean duration of...
Tipo: Text Palavras-chave: Oyster; Ostrea edulis; Brooding species; Sperm; Spermatozeugmata; Endangered species.
Ano: 2018 URL: http://archimer.ifremer.fr/doc/00454/56539/58245.pdf
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Caracteristiques ultrastructurales du spermatozoide de la coquille Saint-Jacques, Pecten maximus (Mollusque, Bivalve) ArchiMer
Dorange, G; Lepennec, M.
The spermatozoon of Pecten maximus is of the primitive type usually associated with species having external fertilization or emitting the sperm in the water. The size of the nucleus is reduced. The middle piece is similar to that reported for several Bivalves. It contains glycogen. But the spermatozoon of P. maximus exhibits its own ultrastructural characteristics: the short acrosome differs in size, shape, position and ultrastructure or by a combination of these from that of many bivalves. No axial rods was observed in contrast to several species of Mytilidae, Veneridae and Ostreidae. These originalities could go to the genus level.
Tipo: Text Palavras-chave: Pecten maximus; Bivalvia; Ultrastructure; Sperm.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/acte-1725.pdf
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Changes in Atlantic cod (Gadus morhua L.) sperm quality during the spawning season ArchiMer
Rouxel, Catherine; Suquet, Marc; Cosson, J; Severe, Armelle; Quemener, Loic; Fauvel, Christian.
The biology of cod reproduction is well described in the scientific literature. However, sperm biology and spermatozoa management are poorly studied in this species. Because of its recent farming expansion, a better knowledge of cod gametes is becoming especially useful. This work aimed at establishing tools to study sperm biology in cod, and also investigated the existence of changes in cod sperm quality during the spawning period. We showed that sperm concentration could be assessed using spectrophotometry at 260 nm. Sperm motility significantly decreased after a 168-h storage at 4 degrees C. A 1:9 dilution of sperm in a non-activating medium (1/3 seawater and 2/3 freshwater, osmotic pressure: 360 mOsm kg(-1)) improved sperm storage. Sperm concentration,...
Tipo: Text Palavras-chave: Spermatozoa; Quality; Ageing; Sperm; Cod; Gadus morhua.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3829.pdf
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Changes in motility, ATP content, morphology and fertilisation capacity during the movement phase of tetraploid Pacific oyster (Crassostrea gigas) sperm ArchiMer
Suquet, Marc; Labbe, C.; Brizard, Raphael; Donval, Anne; Le Coz, Jean-rene; Quere, Claudie; Haffray, P..
Changes in sperm features during the movement phase are especially interesting to study in external fertilization species whose sperm duration movement is long because this implies a significant adaptation of moving cells to the external medium. This study describes the changes in tetraploid Pacific oyster sperm characteristics in relation to time post activation. Sperm individually collected on three tetraploid males were activated in seawater. Their features were analysed over a 24 h period and compared to a sperm pool collected on three diploid males as a reference. The percentage of motile spermatozoa, the intracellular ATP content, and the fine structure of spermatozoa were studied in relation to time post activation. Furthermore, the fertilisation...
Tipo: Text Palavras-chave: Crassostrea gigas; Sperm; Tetraploid; Motility; ATP; Ultrastructure.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00006/11756/8639.pdf
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Characterization of sperm motility in sea bass: the effect of heavy metals and physicochemical variables on sperm motility ArchiMer
Abascal, F; Cosson, J; Fauvel, Christian.
Computer assisted sperm analysis (CASA) was used to characterize the motility of sea bass Dicentrarchus labrax spermatozoa and to study the effect of several physicochemical variables and heavy metals on sperm swimming performance. Duration of sperm motility in sea bass was very short (< 50 s). During the first 20 s all the motility variables measured remained approximately constant, the velocity and linearity of the movement being maximum during this period, while both variables decreased sharply later. While slight variations in pH did not significantly modify sperm swimming performance, changes in osmolality affected all the measured motility variables. Two of the heavy metals tested, Cu2+ and Pb2+, did not affect sperm motility when the activating...
Tipo: Text Palavras-chave: Sperm; Heavy metals; Dicentrarchus labrax; CASA.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-4765.pdf
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Comparative description and discussion of spermiogenesis and spermatozoal ultrastructure in some species of Heptapteridae and Pseudopimelodidae (Teleostei: Siluriformes) Neotropical Ichthyology
Quagio-Grassiotto,Irani; Spadella,Maria Angélica; Carvalho,Márcio de; Oliveira,Claudio.
The data obtained in the present study on spermiogenesis and spermatozoal ultrastructure of Pseudopimelodidae and Heptapteridae show that they share some characteristics, but greatly differ from each other. The main differences are the occurrence of type I spermiogenesis in Pseudopimelodidae and type III in Heptapteridae, the presence of nuclear fossa in Pseudopimelodidae and its absence in Heptapteridae, the presence of long midpiece in Pseudopimelodidae and short midpiece in Heptapteridae, the presence of cytoplasmic canal in Pseudopimelodidae and its absence in Heptapteridae, the presence of many large vesicles in the midpiece of Pseudopimelodidae and the presence of very long vesicles placed in the peripheral distal region in Heptapteridae, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Catfish; Spermatid; Sperm; Structure; Phylogeny.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252005000300008
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Cycles sexuels de quelques poissons plats des cotes bretonnes. ArchiMer
Lahaye, Jacqueline.
Representative species of the families Pleuronectidae, Bothidae and Soleidae were studied throughout the year. Changes are described which were seen in the ovaries, and photographs of cross sections of the tissue are shown. The progress of egg release was noted, and in some cases correlated studies were made of gonadal changes in male. The period of egg release was prolonged over 1 one half -2 months for plaice and turbot, and for 6 months for sole. Individual fish showed these extended periods of laying, and the actual months of the year differed for each species. The massive mobilisation of reserves which necessarily accompanied the release of large numbers of eggs was reflected by turbot, in sperm production. In addition, the increase in gonadosomatic...
Tipo: Text Palavras-chave: Gonadosomatic activity; Sperm; Species; Brittany coasts; Flat fishes; Sexual cycles.
Ano: 1972 URL: http://archimer.ifremer.fr/doc/1972/publication-2064.pdf
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Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization Biol. Res.
Cohen,Débora J; Maldera,Julieta A; Weigel Muñoz,Mariana; Ernesto,Juan I; Vasen,Gustavo; Cuasnicu,Patricia S.
Epididymal protein CRISPI is a member of the CRISP (Cysteine-RIch Secretory proteins) family and is involved in sperm-egg fusion through its interaction with complementary sites on the egg surface. Results from our laboratory have shown that this binding ability resides in a 12-amino-acid region corresponding to a highly conserved motif of the CRISP family, named Signature 2 (S2). In addition to this, our results revealed that CRISP1 could also be involved in the previous step of sperm binding to the zona pellucida, identifying a novel role for this protein in fertilization. As another approach to elucidate the participation of CRISP1 in fertilization, a mouse line containing a targeted disruption of CRISP1 was generated. Although CRISP1-deficient mice...
Tipo: Journal article Palavras-chave: Sperm; Gamete fusion; CRISP; Egg; Fertilization.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200004
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Effect of Diet Contains Sesame Seed on Epididymal Histology of Adult Rat International Journal of Morphology
Mahabadi J,Amini; Khodayari,M; Bafrani H,Hassani; Nikzad,H; Taherian,A.
Sesamin is a major lignan constituent of sesame seed and considered as a key factor in a number of beneficial effects on human health. Sesame leaves intake improve and increase epididymal spermatocytes reserve in adult male sprague dawley rat. The present study was aimed to investigate the effects of contain sesame seed on epididymis histologhical structure. Thirty adult male rats were divided into two groups of 15 rats each. The regimen group received diet containing 30% sesame seed, while the control group received diet along 12 weeks. The right epididymis were removed and minced into several pieces on a specimen bottle containing normal saline for some few minutes to allow the sperms to become motile and swim out and sperm parameters were analyzed. Left...
Tipo: Journal article Palavras-chave: Diet; Epididymis; Sesame seed; Sperm; Hormone.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100042
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Effect of Sesame-Supplemented Diet on Prostate and Seminal Vesicle Histology of Adult Rat International Journal of Morphology
Mahabadi,Javad Amini; Bafrani,Hassan Hassani; Nikzad,Hossein.
Infertility is a great concern among the people of reproductive age. The use of natural products obtained from traditional herbs is appealing. Studies show that antioxidants are important in improving male infertility. Thirty adult male rats were randomly divided into two regimen and control groups. The regimen group received diet containing 30 % sesame seed, while the control group received standard diet for 12 weeks. Histology of prostate and seminal vesicle were evaluated and serum levels of FSH, LH and testosterone concentrations were assessed as well. The results showed that, the diameter of peripheral epithelium and the volume density of the prostate epithelium increased but the volume density of the prostate stroma was decreased significantly in the...
Tipo: Journal article Palavras-chave: Prostate; Seminal vesicle; Sesame seed; Sperm; Hormone.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000200030
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Endothelin-1 downregulates sperm phagocytosis by neutrophils in vitro: a physiological implication in bovine oviduct immunity OAK
MAREY, Mohamed Ali; YOUSEF, Mohamed Samy; LIU, Jinghui; MORITA, Kazuhiro; SASAKI, Motoki; HAYAKAWA, Hiroyuki; SHIMIZU, Takashi; ELSHAHAWY, Ibrahim I.; MIYAMOTO, Akio; 佐々木, 基樹; 清水, 隆; 宮本, 明夫.
The oviduct is an active contractile tube that provides the proper environment for sperm transport, capacitation and survival. Oviductal contractions are regulated by autocrine/paracrine secretion of several factors, such as prostaglandins (PGs) and endothelin-1 (EDN-1). We have previously shown that during the preovulatory stage, sperm are exposed to polymorphonuclear neutrophils (PMNs) in the bovine oviduct, and the bovine oviduct epithelial cells (BOECs) secrete molecules including PGE2 that suppress sperm phagocytosis by PMNs in vitro. In this study, we investigated the possible effects of EDN-1 on the phagocytic activity of PMNs toward sperm. The local concentrations of EDN-1 in oviduct fluid and BOEC culture medium ranged from 10–10 to 10–11 M as...
Palavras-chave: Endothelin-1; Oviduct; Phagocytosis; Polymorphonuclear neutrophils (PMN); Sperm.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4310
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Experiences de reproduction artificielle de Loup Dicentrarchus labrax (Linne 1758). ArchiMer
Barnabe, G.; Tournamille, J.-c..
Mature eggs were expressed from captured female and fertilised artificially with slightly bloody sperm, in dry conditions or in a humid atmosphere, both at 13 degree C. The best incubation conditions proved to be 13 degree C, with streptomycin and penicillin. Another sample was kept at 18 degree C. Hatching occurred after 4 or 5 days, larvae showing positive phototropism. The system of renewal of water and artificial food is described. The embryonic reserve drop of lipid disappeared between day 14 and 16. Observations on larvae were like previous ones, but live larvae had larger mouths than had fixed specimens, and it was found that temperatures of 13 degree C or 18 degree C were suitable beyond the egg stage. A sudden change in temperature caused by a...
Tipo: Text Palavras-chave: Temperature; Sperm; Eggs; Dicentrarchus labrax; Artificial reproduction.
Ano: 1972 URL: http://archimer.ifremer.fr/doc/1972/publication-2063.pdf
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First study in cryopreserved Crassostrea angulata sperm ArchiMer
Riesco, Marta F.; Felix, Francisca; Matias, Domitilia; Joaquim, Sandra; Suquet, Marc; Cabrita, Elsa.
Sperm cryopreservation is a widely employed technique that promotes alternative techniques to contribute to broodstock management or restoration programs for species of commercial interest, endangered species or species with an interesting genotype. The preservation of genetic material from improved stocks or from the original population is extremely important for the oyster aquaculture industry to prevent the potential impacts of epidemic diseases and natural disasters. The Portuguese oyster, Crassostrea angulata, was the most important species commercialized by the shellfish industry. However, inadequate management of this industry and pathology occurrences resulted in a significant decrease in natural populations. For this reason, in this work a...
Tipo: Text Palavras-chave: Crassostrea angulata; Sperm; Cryopreservation; Motility; Viability; Fertilization.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00333/44432/44098.pdf
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"Freezebass" Setting up of a sperm cryobank for sea bass. Q5CT-2002-71209. Final report (01 January 2003 – 30 June 2005) ArchiMer
Chatain, Beatrice; Poline, Olivier.
Objectives : Presently, most of marine fish farmers around the Mediterranean basin grow genetically wild fish strains, which have not been genetically improved for culture performance (growth, appearance, disease resistance etc.). It is inevitable that the present gap for genetically improved strains of marine fish, and their reliable supply, will be filled. It will ultimately dominate the competitive component of marine culture industries once they evolve into commodity-style agribusiness (with fish prices being set at the lowest possible cost of production and the best quality). Those countries, which can competitively develop and supply the improved strains, will enjoy the benefit (perhaps survival) for their local industries. They will avoid...
Tipo: Text Palavras-chave: Pathogen free; Genetic origin; Sperm; Cryopreservation; Sea bass.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/rapport-6494.pdf
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Induced reproduction of dourado (Salminus brasiliensis): fertilization with sperm cryopreserved in ACP®-104 Animal Sciences
Zanandrea, Ana Carolina Volpato; Weingartner, Marcos; Zaniboni-Filho, Evoy.
Experiments were conducted to improve the fertilization rate of cryopreserved semen of dourado, Salminus brasiliensis. The experiment tested two cryoprotectant solutions at different semen: cryoprotectant ratios (1:5, 1:15, 1:25 and 1:50). The standard solution for the species (mixture of dimethyl sulfoxide, glucose, egg yolk and distilled water) was compared to a 350 mOsm ACP®-104 solution, which is composed of powdered coconut water diluted in distilled water and methylglycol. Differences between the dilutions tested were significant only for ACP®. The fertilization potential by using the standard solution at the lowest dilution (1:5) is equated when the sperm is diluted in ACP® at 1:25 or 1:50. These results show that the standard solution is the most...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.06.03.04-3 cryopreservation; Sperm; Fertilization; Cryoprotectant; Coconut water.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/28838
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Long-term effects of the cryopreservation of turbot (Psetta maxima) spermatozoa ArchiMer
Suquet, Marc; Dreanno, Catherine; Petton, Bruno; Normant, Yvon; Omnes, Marie-helene; Billard, Roland.
The survival of turbot eggs and the rearing capacities of larvae stemmed from artificial fertilization practices using frozen-thawed spermatozoa were evaluated. Furthermore, the viability of sperm samples stored during a 9 month period in liquid nitrogen was assessed. No significant difference in the fertilization rate, hatching rate, survival and wet weight of 10-day old larvae were observed using fresh or frozen-thawed spermatozoa. The motility recorded at 10 s and 60 s post-activation and the fertilization capacity of frozen-thawed spermatozoa were not significantly decreased during a 9 month storage period in liquid nitrogen. These results confirm the high quality of the turbot spermatozoa stemmed from the cryopreservation process, allowing their use...
Tipo: Text Palavras-chave: Turbot; Psetta maxima; Sperm; Cryopreservation; Hatching.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00176/28721/27176.pdf
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Melatonin Protective Role in Mouse Cauda Epipidymal Spermatozoa Damage Induced by Sodium Arsenite International Journal of Morphology
Bustos-Obregón,Eduardo; Poblete,Daniel; Catriao,Roberto; del Sol,Mariano; Fernandes,Fábio Henrique.
We evaluated the sperm parameters such as cauda epididymis weight, sperm count, sperm morphology and sperm DNA stability of adult CF-1 male mice treated daily (oral exposure) with the toxic sodium arsenite (As, 7.0 mg/kg/body weight); Melatonin (Me, 10.0 mg/kg/bw), Me (10.0 mg/kg/bw) plus As (7.0 mg/kg/bw) and Negative Control (NaCl 0.9%) to assess acute (8.3 days), chronic (33.2 days) and recovery of testicular damage (66.4 days). Arsenic decreases the number of sperm from chronic treatment (33.2 days) and this effect continued until 66.4 days of treatment. The toxic effect of As also altered the morphology of spermatozoa in all treatment periods when compared to the negative control group. However, Metalonin induced protective effects in periods of 33.2...
Tipo: Journal article Palavras-chave: Mouse; Melatonin; Arsenic; Sperm; DNA stability.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000400017
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Métabolisme énergétique du spermatozoïde d'huître creuse (Crassostrea gigas) ArchiMer
Boulais, Myrina.
The present study aims at describing the energy metabolism of Pacific oyster, Crassostrea gigas, spermatozoon. The main objectives are: (1) describing the kinetic of cellular and biochemical characteristics of Pacific oyster spermatozoon during its movement phase, (2) identifying the metabolic pathways to produce energy and their substrates, (3) determining the involvement of energy metabolism in fertilization success. The results showed the long duration (ranging 24 to 72h) of the movement of Pacific oyster spermatozoon and the maintenance of a high intracellular ATP content until the end of the movement phase. The role of oxidative phosphorylation (OXPHOS) in ATP production was highlighted. Alternative ATP producing pathways might contribute to sustain...
Tipo: Text Palavras-chave: Fécondation; Sperme; Analyse du mouvement; Métabolisme énergétique; RMN; Cytométrie en flux; Crassostrea gigas; Reproduction; Fertilization; Sperm; Movement analysis; Energetic metabolism; NMR; Flow cytometry.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00498/60940/64334.pdf
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