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Registros recuperados: 29
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13CO2 recovery fraction in expired air of septic patients under mechanical ventilation BJMBR
Martins,M.A.; Coletto,F.A.; Martins-Filho,O.A.; Marchini,J.S.; Basile-Filho,A..
The continuous intravenous administration of isotopic bicarbonate (NaH13CO2) has been used for the determination of the retention of the 13CO2 fraction or the 13CO2 recovered in expired air. This determination is important for the calculation of substrate oxidation. The aim of the present study was to evaluate, in critically ill patients with sepsis under mechanical ventilation, the 13CO2 recovery fraction in expired air after continuous intravenous infusion of NaH13CO2 (3.8 µmol/kg diluted in 0.9% saline in ddH2O). A prospective study was conducted on 10 patients with septic shock between the second and fifth day of sepsis evolution (APACHE II, 25.9 ± 7.4). Initially, baseline CO2 was collected and indirect calorimetry was also performed. A primer of 5 mL...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Recovery fraction; 13CO2; Stable isotope; Septic shock; Intensive care setting.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700003
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15N-labed glycine synthesis Anais da ABC (AABC)
Tavares,Claudinéia R.O.; Bendassolli,José A.; Coelho,Fernando; Sant'ana Filho,Carlos R.; Prestes,Clelber V..
This work describes a method for 15N-isotope-labeled glycine synthesis, as well as details about a recovery line for nitrogen residues. To that effect, amination of alpha-haloacids was performed, using carboxylic chloroacetic acid and labeled aqueous ammonia (15NH3). Special care was taken to avoid possible 15NH3 losses, since its production cost is high. In that respect, although the purchase cost of the 13N-labeled compound (radioactive) is lower, the stable tracer produced constitutes an important tool for N cycling studies in living organisms, also minimizing labor and environmental hazards, as well as time limitation problems in field studies. The tests were carried out with three replications, and variable 15NH3(aq) volumes in the reaction were used...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stable isotope; Glycine; Amino acid.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000300005
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15N-labeled glyphosate synthesis and its practical effectiveness Scientia Agricola
Tavares,Claudinéia Raquel de Oliveira; Bendassolli,José Albertino; Ribeiro,Daniela Neves; Rossete,Alexssandra Luiza Rodrigues Molina.
Glyphosate is the herbicide with the greatest participation in the world market, and it has been used intensively in agriculture for over 30 years, especially because of its relatively low cost and high effectiveness on different species. This study describes a synthesis method of glyphosate in the form of the isopropylamine salt enriched in the stable nitrogen isotope (15N). The 15N-herbicide synthesis was performed using a phosphomethylation reaction with dialkyl phosphite and 15N-glycine. The tests were conducted at a microscale and equimolar quantities. A 20% yield was obtained at the established conditions. To test the effectiveness of the synthesized glyphosate, an experiment was performed in a growth chamber with the weed Lolium multiflorum...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Lolium multiflorum; Isopropylamine salt; Stable isotope.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000100014
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Bacterivory by benthic organisms in sediment : Quantification using 15N-enriched bacteria ArchiMer
Pascal, P.y.; Dupuy, C; Mallet, C; Richard, P; Niquil, N.
The fate of benthic bacterial biomass in benthic food webs is a topic of major importance but poorly described. This paper describes an alternative method for evaluation of bacterial grazing rate by meiofauna and macrofauna using bacteria pre-enriched with stable isotopes. Natural bacteria from the sediment of an intertidal mudflat were cultured in a liquid medium enriched with 15NH4Cl. Cultured bacteria contained 2.9% of 15N and were enriched sufficiently to be used as tracers during grazing experiments. Cultured bacteria presented a biovolume (0.21 μm3) and a percentage of actively respiring bacteria (10%) similar to those found in natural communities. The number of Operational Taxon Units found in cultures fluctuated between 56 and 75% of that...
Tipo: Text Palavras-chave: Tracer; Stable isotope; Sediment; Grazing; Bacteria.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4296.pdf
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Characterization of food web structure of the upper continental slope of the Celtic Sea highlighting the trophic ecology of five deep-sea fishes ArchiMer
Kopp, Dorothee; Robert, Marianne; Pawlowski, Lionel.
In marine ecosystems, the study of trophic relationships has extensively benefited from the development of stable isotope analyses (SIA) as dietary tracers. SIA are particularly useful in elucidating the structure of deep sea food webs given the constraints involved in obtaining gut-content data from deep trawling. We used carbon and nitrogen stable isotope analyses and Stable Isotope Bayesian Ellipses in R (SIBER) and Stable Isotope Analysis in R (SIAR) routines, to determine the trophic ecology of five deep-sea fishes from the upper continental slope of the Celtic Sea. SIA made it possible to deduce some general tendencies in food-web structure and species trophic interactions and confirmed diet determined by gut-content analysis for the same species, in...
Tipo: Text Palavras-chave: Competition; Feeding habits; Mixing models; Stable isotope.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00410/52138/52940.pdf
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Contribution of Eucalyptus Harvest Residues and Nitrogen Fertilization to Carbon Stabilization in Ultisols of Southern Bahia Rev. Bras. Ciênc. Solo
Oliveira,Fernanda Cristina Caparelli; Silva,Ivo Ribeiro; Ferreira,Gabriel William Dias; Soares,Emanuelle Mercês Barros; Silva,Sérgio Ricardo; Silva,Eulene Francisco.
ABSTRACT: Eucalyptus forests in southern Bahia (BA) are planted in soils with a sandy surface layer and humid tropical climate, conditions that lead to soil carbon (C) decomposition. Recent studies have shown that nitrogen (N) may be important for soil C stabilization. The aim of this study was to evaluate the contribution of Eucalyptus harvest residues and nitrogen fertilization to C stabilization in Ultisols of southern BA. The experiment was conducted in Eucalyptus clonal plantations cultivated in two regions of Eunápolis, BA, Brazil, with different clay content: southern region (140 g kg-1 of clay) and western region (310 g kg-1 of clay). Five treatments were evaluated: one control (CTR), without Eucalyptus harvest residues and N fertilization, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Planted forests; Harvest residue management; Soil organic matter; Stable isotope.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832018000100501
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Devenir des bactéries dans les réseaux trophiques des vasières intertidales : le cas de brouage (Baie de Marennes-Oléron) ArchiMer
Pascal, Pierre-yves.
Bacteria are highly abundant and productive in intertidal mudflat sediments. By analogy with pelagic system, it has been suggested that bacteria play an important role in benthic food web. Due to technical difficulties, numbers of studies dealing with benthic bacterivory are not numerous and give conflicting results. A method using community of 15N enriched bacteria as tracer was developed in order to measure grazing of meiofauna and macrofauna. Since enriched bacteria present characteristics of size, activity and diversity close to natural ones, bias due to selective bacterial ingestion by grazers are limited. Due to tidal cycles, intertidal mudflat constitutes highly variable environment at short time scale. Since those variations may influence...
Tipo: Text Palavras-chave: Stable isotope; Hydrobia ulvae; Foraminifera; Copepod; Nematode; Bacteria; Mudflat; Trophic foodweb; Isotope stable; Hydrobia ulvae; Foraminifère; Copépode; Nématode; Bactérie; Vasière; Réseau trophique.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/these-4778.pdf
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Effect of alcohol and formaldehyde on the δ13C and δ15N isotopic composition of Plagioscion squamosissimus and Hypophthalmus edentates (Pisces, Osteichthyes) - doi: 10.4025/actascibiolsci.v33i4.70 Biological Sciences
Manetta, Gislaine Iachstel; UEM; Benedito, Evanilde; UEM, Maringá; Ducatti, Carlos; UNESP.
The present study investigates the δ13C and δ15N isotopic composition in frozen samples (control), samples in alcohol and in formaldehyde of Plagioscion squamosissimus and Hypophthalmus edentates. From each individual we extracted a strip of muscle from the region above the lateral line, in the dorsal fin base, that was divided into three equal parts, each one was submitted to one type of treatment: freeze – control group (-15oC), conservation in alcohol 70% and fixation in formaldehyde 4%. Samples were kept under those treatments for 30 days, washed and submerged in distilled water for 4 hours. Afterwards, they were dried up in air oven at 60oC for 48 hours and macerated until the obtaining of a fine powder. A significant difference...
Tipo: Composição isotópica Palavras-chave: Stable isotope; Preservative; Fishes; Plagioscion squamosissimus; Hypophthalmus edentatus 2.05.00.00-9 Ecologia stable isotope; Preservative; Fishes; Plagioscion squamosissimus; Hypophthalmus edentatus.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/7045
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Facing the necessity of describing estuarine ecosystems: a review of food web ecology study techniques ArchiMer
Pasquaud, Stéphanie; Lobry, Jeremy; Elie, Pierre.
Estuarine areas are sites of human pressures and degradation. In order to maintain and/or restore the quality of estuarine ecosystems, it is necessary to describe their structure and functioning. For that reason, many recent scientific works focus on food webs, which are depicted as being good indicators of the functioning of aquatic ecosystems. Hence it is necessary to question how estuarine food webs can be described. This paper proposes a pragmatic and practical review of the most widely used techniques (stomach/gut content analysis, stable isotope ratios and biochemical markers) with emphasis on their main advantages, drawbacks and bias according to possible ecological goals (ecological quality objectives). These approaches, although quite different,...
Tipo: Text Palavras-chave: Trophic model; Biochemical marker; Stable isotope; Stomach/gut content analysis; Estuarine food web.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2784.pdf
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Fertilizer 15N balance in a coffee cropping system: a case study in Brazil Rev. Bras. Ciênc. Solo
Fenilli,Tatiele Anete Bergamo; Reichardt,Klaus; Favarin,José Laércio; Bacchi,Osny Oliveira Santos; Silva,Adriana Lúcia; Timm,Luis Carlos.
Knowledge about the fate of fertilizer nitrogen in agricultural systems is essential for the improvement of management practices in order to maximize nitrogen (N) recovery by the crop and reduce N losses from the system to a minimum. This study involves fertilizer management practices using the 15N isotope label applied in a single rate to determine the fertilizer-N balance in a particular soil-coffee-atmosphere system and to deepen the understanding of N plant dynamics. Five replicates consisting of plots of about 120 plants each were randomly defined within a 0.2 ha coffee plantation planted in 2001, in Piracicaba, SP, Brazil. Nine plants of each plot were separated in sub-plots for the 15N balance studies and treated with N rates of 280 and 350 kg ha-1...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffee crop; Nitrogen uptake; N dynamics; 15N tracer studies; Stable isotope.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832008000400010
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Growth, development, and fertilizer-15N recovery by the coffee plant Scientia Agricola
Fenilli,Tatiele Anete Bergamo; Reichardt,Klaus; Dourado-Neto,Durval; Trivelin,Paulo César Ocheuze; Favarin,José Laercio; Costa,Flávio Murilo Pereira da; Bacchi,Osny Oliveira Santos.
The relationship between growth and fertilizer nitrogen recovery by perenial crops such as coffee is poorly understood and improved understanding of such relations is important for the establishment of rational crop management practices. In order to characterize the growth of a typical coffee crop in Brazil and quantify the recovery of 15N labeled ammonium sulfate, and improve information for fertilizer management practices this study presents results for two consecutive cropping years, fertilized with 280 and 350 kg ha-1 of N, respectively, applied in four splittings, using five replicates. Shoot dry matter accumulation was evaluated every 60 days, separating plants into branches, leaves and fruits. Labeled sub-plots were used to evaluate N-total and 15N...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffea arabica L.; Stable isotope; Nitrogen; Dry matter accumulation; 15N.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000500012
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Influences of geothermal sulfur bacteria on a tropical coastal food web ArchiMer
Pascal, Pierre-yves; Dubois, Stanislas; Goffette, Anais; Lepoint, Gilles.
The activity of the geothermal plant at Bouillante in Guadeloupe (French West Indies) releases thioautotrophic bacteria into the coastal environment. Fish counts reveal that fish abundance increases with higher availability of this bacterial resource. In order to evaluate the trophic role of these bacteria, isotopic compositions (C, N, S) of potential consumers were evaluated on transects at increasing distance from the source of bacteria. The 3 mobile fish species examined (Abudefduf saxatilis, Acanthurus bahianus, and Stegastes partitus) ingested and assimilated chemosynthetic bacteria. Similarly, the isotopic composition of the mobile sea urchin Diadema antillarum was different close to the discharge channel, suggesting a diet mainly composed of sulfur...
Tipo: Text Palavras-chave: Shallow vent; Food web; Sulfur bacteria; Stable isotope.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00404/51511/52256.pdf
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Isotope determination of sulfur by mass spectrometry in soil samples Rev. Bras. Ciênc. Solo
Rossete,Alexssandra Luiza Rodrigues Molina; Carneiro,Josiane Meire Toloti; Sant Ana Filho,Carlos Roberto; Bendassolli,José Albertino.
Sulphur plays an essential role in plants and is one of the main nutrients in several metabolic processes. It has four stable isotopes (32S, 33S, 34S, and 36S) with a natural abundance of 95.00, 0.76, 4.22, and 0.014 in atom %, respectively. A method for isotopic determination of S by isotope-ratio mass spectrometry (IRMS) in soil samples is proposed. The procedure involves the oxidation of organic S to sulphate (S-SO4(2-)), which was determined by dry combustion with alkaline oxidizing agents. The total S-SO4(2-) concentration was determined by turbidimetry and the results showed that the conversion process was adequate. To produce gaseous SO2 gas, BaSO4 was thermally decomposed in a vacuum system at 900 ºC in the presence of NaPO3. The isotope...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sample preparation; Soil samples; Isotopic dilution; Labeled material; Stable isotope; 34S.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832012000600012
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Isotopic signatures (δ 13C and δ15N) of muscle, carapace and claw in Phrynops geoffroanus (Testudines: Chelidae) Rev. Bras. Zool.
Marques,Thiago S.; Tassoni-Filho,Maurício; Ferronato,Bruno O.; Guardia,Isabela; Verdade,Luciano M.; Camargo,Plínio B. de.
The isotopic composition ( δ13C and δ 15N) of muscle, carapace and claw was determined from six wild individuals of Phrynops geoffroanus (Schweigger, 1812) in order to verify the variation between those tissues. The mean values of δ13C e δ 15N were, respectively, -19.48 ± 0.81‰ (-20.8 - -18.64‰) and 7.23 ± 0.67‰ (6.49 - 8.3‰) for muscle, -16.52 ± 0.98‰ (-17.88 - -15.43‰) and 7.29 ± 0.54‰ (6.74 - 7.97‰) for carapace and -18.57 ± 0.97‰ (-19.97 - -17.26‰) and 4.36 ± 0.33‰ (3.93 - 4.79‰) for claw. We found a significant difference for the tissues evaluated for both δ13C and δ15N. Muscle and claw were statistically similar and more depleted in 13C than the carapace. Nonetheless, claw was more depleted in 15N than muscle and carapace tissues. These results are...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diet; Freshwater turtles; Geoffroy's side-necked-turtle; Metabolic processes; Stable isotope.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702011000300016
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Lipid and lipid carbon stable isotope composition of the hydrothermal vent shrimp Mirocaris fortunata: evidence for nutritional dependence on photosynthetically fixed carbon ArchiMer
Pond, David W; Segonzac, Michel; Bell, Michael V; Dixon, David R; Fallick, Anthony E; Sargent, John R.
Mirocaris fortunata were sampled from the Lucky Strike hydrothermal vent area (Eiffel Tower site) on the mid-Atlantic ridge during the French DIVA 2 cruise (June 1994). Small adults (17 to 22 mm total length), although morphologically identical, could be divided into 2 categories on the basis of pigmentation, lipid composition and C-13/C-12 stable isotope ratios of fatty acids. Highly pigmented small adults (8.6 to 9.2 mu g carotenoid shrimp(-1)) contained higher levels of total lipid than similar-sized individuals containing lower levels of pigment (0.9 to 2.9 mu g carotenoid shrimp(-1)). Lipid class analysis indicated that wax esters comprised 62.5% of total lipid in the former group. These pigmented shrimp also contained high proportions of...
Tipo: Text Palavras-chave: Mirocaris fortunata; Lipid; Stable isotope; Hedge betting.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00337/44783/44491.pdf
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Obese women on a low energy rice and bean diet: effects of leucine, arginine or glycine supplementation on protein turnover BJMBR
Marchini,J.S.; Lambertini,C.R.; Ferriolli,E.; Dutra de Oliveira,J.E..
This study examined if leucine, arginine or glycine supplementation in adult obese patients (body mass index of 33 ± 4 kg/m²) consuming a Brazilian low energy and protein diet (4.2 MJ/day and 0.6 g protein/kg) affects protein and amino acid metabolism. After four weeks adaptation to this diet, each subject received supplements of these amino acids (equivalent to 0.2 g protein kg-1 day-1) in random order. On the seventh day of each amino acid supplementation, a single-dose 15N-glycine study was carried out. There were no significant differences in protein flux, synthesis or breakdown. The protein flux (grams of nitrogen, gN/9 h) was 55 ± 24 during the nonsupplemented diet intake and 39 ± 10, 44 ± 22 and 58 ± 35 during the leucine-, glycine- and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Obese women; Protein; Glycine; Arginine; Leucine; Stable isotope.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000007
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Origine et devenir de la matière organique au sein du micro-estuaire temporaire de Fiume Santu (L’Agriate, Haute-Corse) ArchiMer
Massey, Jean-laurent.
In relation with meteorological, hydrological and topographic characteristics, Corsica has a very large number of temporarily open/closed micro-estuaries. Agriates contains several of them, including the Fiume Santu’s temporarily micro-estuary. These ecosystems are specific because of their sporadic connection with the sea. The opening and closure depends on both the river flow and the presence of the Posidonia oceanica (L.) Delile “banquettes”. Characterization of the global functioning was based on a multiparameter approach (particulate matter and dissolved inorganic matter descriptors, carbon and nitrogen stable isotope, phytoplankton communities), in order to qualify and quantify the organic matter (OM), along an estuary – coastal zone gradient. The OM...
Tipo: Text Palavras-chave: Corsica; Fiume Santu; Micro-estuaire temporaire; Matière organique; Isotopes stables; Nutriments; Phytoplancton; Corsica; Fiume Santu; Temporarily open/closed micro-estuary; Organic matter; Stable isotope; Nutrients; Phytoplankton.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00276/38691/37188.pdf
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Phloematic mobility of 10Boron in kiwifruit (Actinidia deliciosa) mixedshoots Ciencia e Investigación Agraria
Sotomayor,Carlos; Ruiz,Rafael; Muñoz,Luis.
Boron (B) is an essential micronutrient showing restricted mobility in plants, except in species that synthesize polyols. The ability of kiwifruit to mobilize B through phloem transport has not yet been confirmed, despite its ability to synthesize the polyol myo-inositol. This study examined kiwifruit plants in which boric acid enriched with the stable 10B isotope was applied to the distal leaves of mixed shoots with flowers. At 24, 72 and 144 h, both 10B-treated and un-treated leaves and flowers from treated shoots were sampled and subsequently analyzed via mass spectrometry to determine the resulting 11B/10B ratios. Control leaves and flowers showed a natural ratio, varying between 3.82 and 4.05. In contrast, the ratios in treated leaves were 1.57 at 24...
Tipo: Journal article Palavras-chave: Boron; Foliar absorption; Kiwifruit; Phloem mobility; Stable isotope.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000300015
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Prey preferences of adult sea bass Dicentrarchus labrax in the northeastern Atlantic: implications for bycatch of common dolphin Delphinus delphis ArchiMer
Spitz, Jerome; Chouvelon, Tiphaine; Cardinaud, M.; Kostecki,; Lorance, Pascal.
In the northeastern Atlantic, adult sea bass (Dicentrarchus labrax) is one of largest fish living on the shelf, and this species has important commercial value. However, pelagic trawl fisheries that target sea bass have negative operational interactions with common dolphins (Delphinus delphis). Our goal was to determine the diet of adult sea bass in the Bay of Biscay from stomachcontent and stable-isotope analyses, and explore the dietary overlap between sea bass and common dolphins. We found that sea bass primarily target small pelagic fish, most notably mackerel (Scomber scombrus), scads (Trachurus spp.), anchovy (Engraulis encrasicolus), and sardine (Sardina pilchardus). These four species also dominated the diets of common dolphins. This overlap in...
Tipo: Text Palavras-chave: Chesson's index; Marine top predator; Prey selection; SIAR; Stable isotope; Stomach content.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00125/23625/21485.pdf
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Production of 34S-labeled gypsum (Ca34SO4.2H2O) Scientia Agricola
Rossete,Alexssandra Luiza Rodrigues Molina; Bendassolli,José Albertino; Máximo,Everaldo; Sant Ana Filho,Carlos Roberto; Ignoto,Raquel de Fátima de.
Agricultural gypsum (CaSO4.2H2O) stands out as an effective source of calcium and sulfur, and to control aluminum saturation in the soil. Labeled as 34S it can elucidate important aspects of the sulfur cycle. Ca34SO4.2H2O was obtained by chemical reaction between Ca(OH)2 and H2(34)SO4, performed under slow agitation. The acid was produced by ion exchange chromatography using the Dowex 50WX8 cation exchange resin and a Na2(34)SO4 eluting solution. After precipitation, the precipitate was separated and dried in a ventilated oven at 60ºC. From 2.2 L H2SO4 0.2 mol L-1 and 33.6 g Ca(OH)2, 73.7 ± 0.6 g Ca34SO4.2H2O were produced on average in the tests, representing a mean yield of 94.6 ± 0.8%, with 98% purity. The 34SO2 gas was obtained from Ca34SO4.2H2O in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sulfur 34; Stable isotope; Isotopic determination.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162006000400012
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