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A new sterile technique effective on capturing tramp ants for microbiological investigations Neotropical Entomology
Schuller,Lucia; Matté,Glavur R; Matté,Maria H.
Tramp ants are an outstanding group of organisms that have a definitive and important role in carrying pathogens. The purpose of this study was to develop a microbiological sterile technique of collecting ants from contaminated areas. The traps were composed of Petri dishes containing culture media, and were tested to verify the applicability of the system. A positive correlation between the microorganism growth and the presence of ants inside or around traps was observed. The technique described demonstrated to be useful to collect ants from different environments, helping the surveillance of pathogenic microorganisms that are of public health concern.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Public health; Pathogenic microorganism; Staphylococcus aureus; Klebsiella; Escherichia coli; Enterobacter.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2009000400023
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Actinomicetos produtores de inibidores de β-lactamases com atividade antimicrobiana frente a isolados de mastite bovina Arq. Bras. Med. Vet. Zootec.
Cunha,M.N.C; Silva,N.M.V; Teixeira,M.F.S; Mota,R.A; Lima-Filho,J.L; Porto,T.S; Porto,A.L.F.
Avaliou-se a capacidade de 71 actinomicetos isolados de líquens da região amazônica em produzir inibidores de β-lactamases com atividade antimicrobiana sobre Staphylococcus aureus, resistentes à penicilina, isolados de mastite bovina do estado de Pernambuco. A seleção dos actinomicetos produtores de inibidores de β-lactamases foi realizada pela técnica de bloco de gelose contra Klebsiella pneumoniae ATCC 29665, e os actinomicetos selecionados foram testados frente a 17 linhagens de Staphylococcus aureus resistentes à penicilina. Os melhores produtores de inibidores de β-lactamases foram Streptomyces sp. DPUA 1542 e Nocardia sp. DPUA 1571, os quais foram submetidos ao cultivo submerso para determinação da curva de crescimento, pH e atividade antimicrobiana....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mastite bovina; Inibidor de β-lactamase; Actinomiceto; Atividade antimicrobiana; Staphylococcus aureus.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600003
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Action of nisin and high pH on growth of Staphylococcus aureus and Salmonella sp. in pure culture and in the meat of land crab (Ucides cordatus) BJM
Grisi,Teresa Cristina S. de Lima; Gorlach-Lira,Krystyna.
The aim of this study was to evaluate the potential of nisin and high pH to inhibit the growth of Staphylococcus aureus and Salmonella sp. in broth culture and when inoculated into meat of land crab. In pure cultures, the growth of S. aureus was bly inhibited by nisin and the growth of Salmonella sp. was inhibited by nisin-EDTA (20 mM). The inhibition of S. aureus lasted for eight hours and Salmonella sp. growth was inhibited throughout the experiment (24 h). The high pH (pH 10.0 and 11.0 with NaHCO3-NaOH buffer) was very effective for in vitro inhibition of S. aureus and Salmonella sp. Nisin and high pH, when applied to the contaminated meat, did not yield the same effect. Nisin was not effective in preventing growth of both pathogens in the crab meat,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Crab meat; Nisin; High pH; Staphylococcus aureus; Salmonella sp.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200010
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Action of peracetic acid on Escherichia coli and Staphylococcus aureus in suspension or settled on stainless steel surfaces BJM
Kunigk,Leo; Almeida,Maria C.B..
The efficiency of a commercial peracetic acid sanitizer on destruction of Staphylococcus aureus and Escherichia coli was evaluated using two distinct methods. The first method is the AOAC suspension test and the second is a method proposed by one of the authors in which the microbial cells are settled on a stainless steel surface and then treated with the sanitizer. The results showed that when in suspension S. aureus was more resistant to the sanitizer than E. coli. When S. aureus was settled on the stainless steel surface, the contact time between the sanitizer and the microorganisms to attain a 6.5 log reduction in the number of viable cells was three times greater than when the cells were in suspension.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Peracetic acid; Staphylococcus aureus; Escherichia coli; Stainless steel surface; Sanitizers.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100009
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Actividad antiséptica de vinagre de Guadua angustifolia Kunth Plantas Medicinales
Mejía Gallón,Amanda Inés; Cadavid Torres,Elizabeth; Gallardo Cabrera,Cecilia.
Introducción: los vinagres de bambú se utilizan en los países orientales en aplicaciones farmacéuticas, con la especie Guadua angustifolia Kunth no se han encontrado investigaciones similares. Evaluar la actividad antiséptica del vinagre de guadua permitiría avanzar en estos desarrollos. Objetivo: determinar la sensibilidad de las bacterias Pseudomona aeruginosa y Staphylococcus aureus frente al vinagre de guadua, los cambios con la concentración y el pH. Métodos: la actividad antimicrobiana se midió por difusión en agar en medio Muller Hinton. Se ensayaron vinagres a concentraciones entre 100, 80, 60, 40 y 20 % a pH entre 3 y 6. Se estableció la concentración mínima inhibitoria. Resultados: el vinagre de guadua presenta actividad antiséptica a...
Tipo: Journal article Palavras-chave: Pseudomona aeruginosa; Staphylococcus aureus; Ácido acético; Vinagre de Guadua angustifolia Kunth; Actividad antiséptica.
Ano: 2011 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962011000300004
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An alternative method for Staphylococcus aureus DNA isolation. Repositório Alice
CHAPAVAL, L.; MOON, D. H.; GOMES, J. E.; DUARTE, F. R.; TSAI, S. M..
Metodologia alternativa para extração de DNA genômico de Staphylococcus aureus. Abstract: This study describes a rapid procedure for the isolation of genomic DNA from Staphylococcus aureus that yielded a good amount of high quality DNA for the amplification of staphylococcal enterotoxins genes ( A, B, C, D, and E) and the TSST-1 gene as well as enzymatic restriction ( HaeIII) from environmental isolates. With this method, it was possible to detect these genes in a sample containing as little as 105 cells with positive PCR reactions obtained from approximately 10pg of DNA in a final reaction volume of 25 mu l. Metodologia alternativa para extração de DNA genômico de Staphylococcus aureus. Resumo: Descreve-se um procedimento rápido para extração de DNA...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Staphylococcus aureus; DNA genômico; Extração de DNA; Staphylococcus aureus; Genomic DNA; DNA isolation.
Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/handle/doc/534206
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An alternative method for Staphylococcus aureus DNA isolation Arq. Bras. Med. Vet. Zootec.
Chapaval,L.; Moon,D.H.; Gomes,J.E.; Duarte,F.R.; Tsai,S.M..
This study describes a rapid procedure for the isolation of genomic DNA from Staphylococcus aureus that yielded a good amount of high quality DNA for the amplification of staphylococcal enterotoxins genes (A, B, C, D, and E) and the TSST-1 gene as well as enzymatic restriction (HaeIII) from environmental isolates. With this method, it was possible to detect these genes in a sample containing as little as 10(5) cells with positive PCR reactions obtained from approximately 10pg of DNA in a final reaction volume of 25µl.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Genomic DNA; DNA isolation.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200004
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An association between milk and slime increases biofilm production by bovine Staphylococcus aureus. Repositório Alice
FABRES-KLEIN, M. H.; SANTOS, M. J. C.; KLEIN, R. C.; SOUZA, G. N. de; RIBON, A. de O. B..
2015
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Staphylococcus aureus; Biofilm; Slime; Skim-milk medium; Agr type.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1037104
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Análise microbiológica do queijo minas artesanal do Serro-MG, Brasil, produzido com e sem fermento natural MV&Z
Cunha, A. L. F. S.; Paiva, V. N.; Cabrini, C. C.; Vieira, P.; Pinto, M. S..
Os queijos Minas artesanais possuem grande importância econômica, social, ambiental e política para o estado de Minas Gerais, sendo responsáveis pelo sustento direto e indireto de mais de 35 mil famílias. Até o presente momento não existem estudos envolvendo a utilização do fermento natural na fabricação de queijos Minas artesanais. Sabe-se apenas que o mesmo é utilizado de forma indiscriminada em algumas regiões produtoras tradicionais onde alguns produtores utilizam poucos mililitros para cada 100 litros de leite enquanto outros utilizam até um litro para os mesmos 100 litros. Além disso, segundo os produtores, as perdas de produção são atribuídas à qualidade desse fermento natural. O presente trabalho avaliou o efeito da adição de fermento natural sobre...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Segurança Alimentar; Coliformes; Escherichia coli; Staphylococcus aureus.
Ano: 2016 URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/34810
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Antibacterial activity and chemical composition of essential oil of Lippia microphylla Cham - doi: 10.4025/actascibiolsci.v33i2.5792 Biological Sciences
Rodrigues, Fabiola Fernandes Galvão; Universidade Regional do Cariri; Coutinho, Henrique Douglas Melo; Universidade Regional do Cariri; Campos, Adriana Rolim; Universidade Regional do Cariri; Lima, Sidney Gonçalo; Universidade Federal do Piauí; Costa, José Galberto Martins; Universidade Regional do Cariri.
The essential oil from the fresh leaves of Lippia microphylla Cham. was obtained by hydrodistillation and characterized by gas chromatography-mass spectrometry (GC-MS). Major constituents of the oil were 1.8-cineole (18.12%), β-ocimene (15.20%), bicyclogermacrene (11.63%) and caryophyllene oxide (8.32%). Antimicrobial activity of the oil against Gram-positive and Gram-negative bacteria was determined by gel diffusion method. The oil showed good antibacterial activity against Shigella flexneri, Escherichia coli and Streptococcus pyogenes and very good antibacterial activity against Staphylococcus aureus.
Palavras-chave: 2.03.06.00-8 antibacterial activity; 1 8-cineole; Verbenaceae; Staphylococcus aureus Botanica Aplicada antibacterial activity; 1 8-cineole; Verbenaceae; Staphylococcus aureus.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5792
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Antibacterial activity of Aroeira honeys produced in Minas-Gerais against bacteria of clinical importance Biological Sciences
Viana, Fabiana Ribeiro; Carmo, Luiz Simeão do; Bastos, Esther Margarida Alves Ferreira.
The biological activity of honey has been the subject of great scientific investigation. Bee products are widely used in folk medicine to treat human diseases due to their antibacterial and inhibitory potential action on different human pathogens. Ten honey samples produced by Apis mellifera in the northern of Minas Gerais state were tested against pathogenic microorganisms Staphylococcus aureus TSST (clinical isolated) and enterohaemorrhagic Escherichia coli ATCC 43895 for determination of their minimum inhibitory concentrations. The microdilution technique in broth Mueller-Hinton broth (MHB) was used in four concentrations (25, 12.5, 6.25 and 3.125%). There was a reduction of bacterial growth for the two target species at all concentrations tested. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bee products; Antibacterial activity; Staphylococcus aureus; Escherichia coli.
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/36766
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Antibacterial effect of borage (Echium amoenum) on Staphylococcus aureus BJID
Abolhassani,Mohsen.
Borage (Echium amoenum) is a large annual plant of the Boraginaceae family, which grows in most of Europe and in northern Iran. The borage flower is used as a medicinal herb in France and other countries. Iranian borage is used in traditional medicine for infectious diseases, flu and as an anti-febrile. We tested the aqueous extract of borage dried flowers in vitro for its antibacterial activity. The extract showed concentration-dependent antibacterial activity against Staphylococcus aureus 8327. This activity was heat resistant, but the activity of freeze-dried extract gradually diminished during a 90-day period. The traditional use of Iranian borage flowers for infectious diseases and for controlling fever appears to be justified.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Borago; Echium amoenum; Antibacterial activity; Staphylococcus aureus.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000500008
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Antibacterial resistance of community-acquired respiratory tract pathogens recovered from patients in Latin America: results from the PROTEKT surveillance study (1999-2000) BJID
Mendes,C.; Marin,M. E.; Quiñones,F.; Sifuentes-Osornio,J.; Cuilty Siller,C.; Castanheira,M.; Zoccoli,C. M.; López,H.; Súcari,A.; Rossi,F.; Barriga Angulo,G.; Segura,A.J.A.; Starling,C.; Mimica,I.; Felmingham,D..
PROTEKT (Prospective Resistant Organism Tracking and Epidemiology for the Ketolide Telithromycin) is a global surveillance study established in 1999 to monitor antibacterial resistance of respiratory tract organisms. Thirteen centers from Argentina, Brazil and Mexico participat ed during 1999-2000; they collected 1,806 isolates (Streptococcus pneumoniae 518, Haemophilus influenzae 520, Moraxella catarrhalis 140, Staphylococcus aureus 351, S. pyogenes 277). Overall, 218 (42.1%) of the S. pneumoniae isolates had reduced susceptibility to penicillin, 79 (15.3%) were penicillin-resistant and 79 (15.3%) were erythromycin-resistant. Mexico had the highest prevalence of penicillin (76.5%) and erythromycin (31.2%) resistance. Of 77 erythromycin-resistant S....
Tipo: Info:eu-repo/semantics/article Palavras-chave: PROTEKT; Latin America; Resistance; Respiratory tract infections; Streptococcus pneumoniae; Haemophilus influenzae; Moraxella catarrhalis; Streptococcus pyogenes; Staphylococcus aureus.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000100006
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Antimicrobial activity of a biosurfactant produced by Bacillus licheniformis strain M104 grown on whey BABT
Gomaa,Eman Zakaria.
The aim of the present study was to investigate the antimicrobial effect of the lipopeptide biosurfactants produced by Bacillus licheniformis strain M104 grown on whey. The biosurfactant was investigated for potential antimicrobial activity by using the disc-diffusion method against different Gram-positive bacteria {B subtilis, B. thuringiensis (two strains), B. cereus, Staphylococcus aureus (two strains) and Listeria monocytogenes)}, Gram-negative bacteria {(Pseudomonas aeruginosa, Escherichia coli (two strains), Salmonella typhimurium, Proteous vulgaris and Klebsiella pneumoniae) and a yeast (Candida albicans)}. The biosurfactant showed profoundly distinct antibacterial activity toward tested bacteria and displayed an antifungal activity against the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biosurfactant; Bacillus licheniformis; Cheese whey; Antimicrobial activity; Staphylococcus aureus.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200011
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Antimicrobial activity of Aspilia latissima (Asteraceae) BJM
Souza,Jeana M.E.; Chang,Marilene R.; Brito,Daniela Z.; Farias,Katyuce S.; Damasceno-Junior,Geraldo A.; Turatti,Izabel C.C.; Lopes,Norberto P.; Santos,Edson A.; Carollo,Carlos A..
Abstract We evaluated the antimicrobial activity of Aspilia latissima - an abundant plant from the Brazilian Pantanal region - against Candida albicans, Candida parapsilosis, Candida krusei, Candida tropicalis, Pseudomonas aeruginosa, Enterococcus faecalis, Escherichia coli and Staphylococcus aureus. The crude extracts and fractions showed activity in all tested microorganisms. The chloroform fraction of the leaves and roots showed the most antimicrobial activity against S. aureus, with an MIC of 500 μg/mL. This fraction was submitted to bioautographic assays to characterize the activity of the compounds. Two bands from the leaves (L-A and L-B) and three bands from the roots (R-C, R-D and R-E) were bioactive. Within the root-derived bands, the terpene...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspilia latissima; Staphylococcus aureus; Bioautographic; MIC; Terpenes.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401103
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Antimicrobial activity of propolis and essential oils and synergism between these natural products J. Venom. Anim. Toxins incl. Trop. Dis.
Probst,IS; Sforcin,JM; VLM,Rall; Fernandes,AAH; Fernandes Júnior,A.
In the present study, Apis mellifera propolis and essential oils (EOs) obtained from aromatic plants were evaluated as alternative antimicrobials. We aimed to establish the antimicrobial activity of ethanolic extracts of propolis (EEP) from Apis mellifera and of EOs from Caryophyllus aromaticus, Zingiber officinale, Cinnamomum zeylanicum and Mentha piperita against 32 Staphylococcus aureus and Escherichia coli strains from human clinical specimens. The antimicrobials were diluted in agar and the minimal inhibitory concentrations (MIC) were found whereas MIC90% values were calculated. Time-kill curve assays using mixtures containing one quarter of MIC90% for EEP with all EOs as well cinnamon EO were performed. The cinnamon EO was found to be the most...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apis mellifera; Aromatic plants; Ethanolic extract of propolis; Staphylococcus aureus; Escherichia coli.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200006
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Antimicrobial effect of turmeric (Curcuma longa) on chicken breast meat contamination Rev. Bras. Ciênc. Avic.
Lourenço,TC; Mendonça,EP; Nalevaiko,PC; Melo,RT; Silva,PL; Rossi,DA.
The aim of this study was to evaluate the efficacy of turmeric (Curcuma longa), also known in Brazil as saffron, on the reduction of Staphylococcus aureus and Escherichia coli counts in chicken meat. Forty breast meat samples were divided in two groups (A and B). In group A, 10³-10(4)E. coli (ATCC 25922) cells were inoculated and group B samples were inoculated with 10(4)-10(5)S. aureus (ATCC 9801) cells, after which each group was divided in three samples. The first sample was analyzed immediately after inoculation. The second sample (control group) was stored at 4 ºC for 48 hours and turmeric at 1% (w/w) was added to the third sample, which was homogenized and then stored under the same conditions as the second sample. E. coli and S. aureus were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Turmeric; Bacterial growth; Escherichia coli; Staphylococcus aureus.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2013000200002
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Antimicrobial effects of plant defence peptides expressed by bovine endothelial cells on intracellular pathogens Electron. J. Biotechnol.
Loeza-Ángeles,Heber; López-Meza,Joel E; Ochoa-Zarzosa,Alejandra.
Background: The actions of plant antimicrobial peptides (PAP) against intracellular pathogens are poorly known. It has been reported that wheat puroindolines show antibacterial activity against Staphylococcus epidermidis endocyted by macrophages. In this work, we evaluated the intracellular antimicrobial activity of PAP gamma-thionin and thionin Thi2.1 produced by bovine endothelial cells against intracellular Staphylococcus aureus and Candida albicans. We used three host-pathogen models: 1) bovine mammary epithelial cells (BMEC)-S. aureus, 2) bovine endothelial cells (BEC)-S. aureus and 3) BEC-C. albicans, and evaluated the effect of conditioned media from BEC producers of PAP (gamma-thionin and thionin Thi2.1). Results: In the first model, conditioned...
Tipo: Journal article Palavras-chave: Antimicrobial peptides; Arabidopsis thaliana thionin; Capsicum chinense gamma-thionin; Candida albicans; Intracellular pathogens; Staphylococcus aureus.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500001
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Antimicrobial susceptibility, virulence determinant carriage and molecular characteristics of Staphylococcus aureus isolates associated with skin and soft tissue infections BJID
Yu,Fangyou; Liu,Yunling; Lv,Jinnan; Qi,Xiuqin; Lu,Chaohui; Ding,Yu; Li,Dan; Liu,Huanle; Wang,Liangxing.
ABSTRACT A better understanding of the antimicrobial susceptibility, carriage of virulence determinants and molecular characteristics of Staphylococcus aureus isolates associated with skin and soft tissue infections (SSTIs) may provide further insights related to clinical outcomes with these infections. From January 2012 to September 2013, a total of 128 non-duplicateS. aureus isolates were recovered from patients with SSTIs. All 128 S. aureus SSTI isolates carried at least five virulence genes tested. Virulence genes detected among at least 70% of all tested isolates included hld (100%), hla (95.3%),icaA (96.9%), clf (99.2%),sdrC (79.7%), sdrD (70.3%), andsdrE (72.7%). The prevalence of MRSA isolates with 10 virulence genes tested (54.4%, 31/56) was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Skin and soft tissue infections; Molecular characteristic; Virulence genes.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000600614
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Aspectos epidemiológicos da mastite bovina causada por Staphylococcus aureus. Repositório Alice
ZAFALON, L. F.; LONGONI, H.; BENVENUTTO, F.; CASTELANI, L.; BROCCOLO, C. R..
A finalidade deste trabalho foi monitorar os casos de mastite bovina e verificar a presença de Staphylococcus aureus com agente etiológicoda doença. A sua presença em diferentes sítios de localização e a relação entre o aparecimento da doença e as características ambientais foram acompanhadas.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Staphylococcus aureus; Matite bovina; Epidemiologia.
Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/handle/doc/48414
Registros recuperados: 216
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